JP3199277B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic comprising a ganoderma extract prepared by a specific extraction method .

[0002]

2. Description of the Related Art Reishi is considered to be the most valuable herbal medicine together with ginseng since ancient times. It is said that Kampo has tonicity, tranquility, diuresis, liver assist, etc., and its effectiveness against various vascular diseases has been recognized in modern medicine.

[0003] In addition, Japanese Patent Application Laid-Open No. 58-113118 discloses that a cosmetic containing a ganoderma extract has a skin conditioning effect, and is excellent in safety and stability over time.

[0004]

However, the effect of Ganoderma lucidum on the skin dermis has not been sufficiently obtained. In addition, although Reishi is used as a cosmetic, the coloration of Reishi itself, which is a problem at that time, has not been improved yet.

[0005] One of the skin aging phenomena is collagen and glucosaminoglycan (GA), which are constituents of the dermis.
G) A decrease in the amount of formation is known, and this is known to greatly affect the occurrence of wrinkles and a decrease in skin elasticity.

[0006] Then, when the ganoderma extract of the present invention was examined using human dermal fibroblast cell culture, it was found that collagen extract was superior to the ganoderma extract in promoting collagen production. Glycan formation was shown to be as effective as a mere Ganoderma extract. In addition, single
By performing fractionation of ganoderma extract made, improvement is seen in the coloring property, found that it is better as a cosmetic formulation agents, and have reached the present invention.

[0007]

SUMMARY OF THE INVENTION The present invention relates to a specific extracting method.
A cosmetic comprising a reishi extract prepared by a method . The term “reishi” in the present invention refers to a mushroom belonging to the family Sarcophaga and includes, for example, Ganoderma lucidum and maggots, and commercially available products can be used.

[0008] The Ganoderma lucidum extract of the present invention can be obtained, for example, as follows. A water-soluble solvent is added to the dried shredded Reishi to extract and alcohol (ethanol, methanol, etc.) is added to the water-soluble extract until the polysaccharide precipitates.
To precipitate the polysaccharide. Examples of the water-soluble solvent include water, ethanol, 1,3-butylene glycol, propylene glycol and the like. Subsequently, in order to remove the polysaccharide as a precipitate, the extract was used as it was.
Alternatively, after refrigerated storage, the extract is subjected to centrifugation or filtration to obtain the Reishi extract of the present invention. Repeat the above operation several times as necessary. The Ganoderma lucidum extract of the present invention can be concentrated or concentrated to dryness, if necessary, to facilitate subsequent operations.

[0009] The Ganoderma lucidum extract of the present invention is 0.001 to 10% by weight, preferably 0.
01 to 5.0% by weight can be blended. At a concentration of 0.001% or less, a sufficient effect cannot be obtained, and at a concentration of 10% by weight or more, no enhancement of the effect is observed, which is uneconomical. In the cosmetic of the present invention, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, and the like used in cosmetics and the like, as long as the effects of the reishi extract of the present invention are not impaired. Raw materials such as surfactants can be blended. The cosmetic of the present invention can employ basic cosmetics such as creams, lotions, emulsions, and packs, makeup cosmetics such as foundations and lipsticks, and bath forms, soaps, and other forms.

[0010]

EXAMPLES Next, examples will be given to explain the present invention in detail.
However, the present invention is not limited to this. still,
The parts of the amounts shown in the examples are parts by weight. Example-1Of the present inventionReishi ExtractProduction method  3L of purified water in 100g of Reishi (dried magojakushi fruit body)
The mixture was extracted at 100 ° C. for 3 hours, cooled, and filtered. To the rest
Was added with 3 L of water, and the mixture was extracted by heating in the same manner. Combine the filtrate
After concentration under reduced pressure, add 6 volumes of ethanol (ethanol
(85% final concentration) polysaccharide was precipitated and centrifuged. That
SupernatantOf the present inventionReishi extract, precipitate reduced as polysaccharide fraction
Pressure concentration and freeze-drying were performed to obtain a powder sample. In addition, d
Vacuum freeze-dried vacuum concentrate before adding ethanol.
ThemereReishi extract was used.

Embodiment 2Of the present inventionReishi ExtractProduction method  3L of purified water in 100g of Reishi (dried body of Mannaketake fruit body)
The mixture was extracted at 100 ° C. for 3 hours, cooled, and filtered. To the rest
Was added with 3 L of water, and the mixture was extracted by heating in the same manner. Combine the filtrate
After concentration under reduced pressure, add 6 volumes of ethanol (ethanol
(85% final concentration) polysaccharide was precipitated and centrifuged. That
SupernatantOf the present inventionReishi extract, precipitate reduced as polysaccharide fraction
Pressure concentration and freeze-drying were performed to obtain a powder sample. In addition, d
Vacuum freeze-dried vacuum concentrate before adding ethanol.
ThemereReishi extract was used.

Example 3 Lotion Formulation Formulation Amount 5.0 parts of ethanol 2. 2. methyl p-oxybenzoate 0.1 Polyoxyethylene (40) hydrogenated castor oil 0.14. Appropriate amount of fragrance 5. Purified water 10.0 6. Ganoderma extract of the present invention (Example -1) 5.0 7.1,3- butylene glycol 3.0 8. Glycerin 2.0 9. Xanthan gum 0.02 10. Purified water 74.5 Production method: Components 1 to 5 and components 6 to 10 are each uniformly dissolved, mixed and filtered to obtain a product.

Comparative Example 1 Conventional Lotion The same method as in Example 3 except that the Ganoderma lucidum extract of the present invention was replaced with a mere Ganoderma lucidum extract was used as a conventional lotion.

Example-4 Cream Formulation Amount 1. 4.0 parts of stearic acid 2. Cetyl alcohol 3.0 Stearyl alcohol 1.0 4. Liquid paraffin 6.5 5. Vaseline 10.0 6. Sorbitan monostearate 2.5 7. 8. Polyoxyethylene (25) monostearate 3.0 8.1 1,3-butylene glycol 5.0 Potassium hydroxide 0.110. Reishi Extract of the Present Invention (Example-2) 0.2 11. 11. methyl p-oxybenzoate 0.2 Purified water 64.5 13. Appropriate amount of fragrance Production method: Oil phase components 1 to 7 and aqueous phase components 8 to 12 are heated and dissolved at 70 to 75 ° C respectively, and then aqueous phase components to oil phase components 1 to 7
Add 8 to 12 to emulsify, add and mix component 13 while cooling, and cool to 30 ° C to obtain a product.

Example-5 Emulsion Formulation Formulation Amount 2.0 parts of stearic acid 2. 2. Stearyl alcohol 2.0 Liquid paraffin 8.0 4. Glycerin monostearate 2.3 5. Sorbitan monooleate 2.5 6. Polyoxyethylene (10) sorbitan monooleate 0.8 7. Glycerin 6.0 8. Reishi extract of the present invention (Example-2) 0.59. 9. Methyl p-oxybenzoate 0.2 Purified water 77.0 11. Appropriate amount of fragrance Production method: After heating and dissolving oil phase components 1 to 7 and aqueous phase components 8 to 10 at 70 to 75 ° C, add aqueous phase components to oil phase components 1 to 7.
Add 8 to 10 to emulsify, add component 11 while cooling, mix and cool to 30 ° C to obtain a product.

Comparative Example 2 Conventional Emulsion A conventional emulsion was prepared by replacing the Ganoderma lucidum extract of the present invention with a mere Ganoderma lucidum extract in Example-5.

Example-6 Pack Formulation Compounding Amount Polyvinyl alcohol 12.0 parts 2. Ethyl alcohol 5.0 3.1,3-butylene glycol 8.04. methyl p-oxybenzoate 0.25. Polyoxyethylene (40) hydrogenated castor oil 0.56. Reishi Extract of the Present Invention (Example-1) 1.0 7. Citric acid 0.18. Sodium citrate 0.39. Appropriate amount of fragrance 10. Purified water 73.8 Production method: Dissolve each component uniformly to obtain a product.

Example-7 Foundation Formulation Compounding Amount 1. Reishi extract of the present invention (Example-2) 0.5 Stearic acid 2.4 3. 3. Polyoxyethylene (20) sorbitan monostearate 1.0 4. Polyoxyethylene (20) cetyl ether 2.0 Cetyl alcohol 1.0 Liquid lanolin 2.0 7. Liquid paraffin 3.0 8. 8. Isopropyl myristate 6.5 10. butyl p-oxybenzoate 0.110. Purified water 58.2 11. Sodium carboxymethyl cellulose 0.112. Bentonite 0.5 13. Propylene glycol 4.0 14. Triethanolamine 1.1 15. 15. methyl p-oxybenzoate 0.216. Titanium oxide 8.0 17. Talc 4.0 18. Colored pigment 5.0 19. Appropriate amount of fragrance Production method: Components 1 and 15 are heated to 70 ° C to swell component 11 well. The component 10 in which the component 12 is dispersed is added thereto and dissolved, and then the components 13 and 14 are dissolved to form an aqueous phase.
Components 2 to 9 are dissolved by heating and kept at 80 ° C to form an oil phase. To the aqueous phase, add the components 16 to 18 that have been mixed well and passed through a pulverizer, and stirred with a homomixer to keep the temperature at 75 ° C. The oily phase is added to the aqueous phase with stirring, cooled, and the component 19 is added at 45 ° C.

Example-8 Bath Agent Formulation Compounding Amount Sodium sulfate 43.0 2. Sodium bicarbonate 50.0 3. 3. Reishi Extract of the Present Invention (Example-1) 5.0 4. Yellow 202 No. (1) Suitable amount Appropriate amount of fragrance Production method: Mix each component well to make a product.

[0020]

The Ganoderma lucidum extract of the present invention has an excellent collagen and glucosaminoglycan production promoting effect on cultured human dermal fibroblasts, and contains the Ganoderma lucidum extract of the present invention. The cosmetic characterized in that it exhibited an excellent skin conditioning effect. Further, the coloring property was also improved. next,
An experimental example will be given to explain the effect of the present invention in detail.

EXPERIMENTAL EXAMPLE 1 Collagen Production-Promoting Activity In the test, human skin fibroblasts (from a 6-year-old male, passage number 6) were used as test cells, and Eagle's MEM was used as a medium. Collagen production was measured using 3H-proline incorporation into collagen protein as an index. 96
25, human skin fibroblasts in a well plate (Falcon 3072)
The cells were seeded at 000 cells / well and cultured in Eagle's MEM containing 10% FCS at 37 ° C. under 5% CO 2 for 4 days. During this time, the cells became confluent. Next, a serum-free Eagle's obtained by simply adjusting a simple Ganoderma extract, a polysaccharide fraction of the Ganoderma extract, and a Ganoderma extract of the present invention (Example 1) to a final concentration of 1 to 10 μg / ml, respectively. MEM (50μg / ml L-ascorbic acid, 50μg / ml β-aminopropionitrile, 18.5KBq
(Containing 3H-proline) and cultured for 24 hours. Then, immediately after adding a pepsin solution to each well and incubating, 3H-proline-labeled collagen was extracted. After purification by salting out, the radioactivity in the collagen protein was determined using a liquid scintillation counter (Beckman
LS 5801). Each sample was measured for 5 wells, and the amount of production was shown as an average value ± standard deviation. The sample without addition was used as a control.

[0022]

[Table 1] Table 1 Collagen synthesis promoting action ··········· Concentration Collagen production Production promotion rate Significant difference [μg / ml] [DPM / 25,000 cells] [%] ----- control 3855.4 ± 289.0 100 spirits just ganoderma extract 1 3890.1 ± 504.3 101 10 4981.2 ± 568.8 129 ** ganoderma extract 1 4435.7 ± 400.8 115 (polysaccharide fraction) 10 3814.7 ± 446.5 99 invention Turf extract 1 4949.9 ± 204.8 128 *** 10 4681.2 ± 182.4 121 *** ···· Significant difference (vs control) **: P <0.005, ***: P <0.001 As shown in Table 1, the Ganoderma lucidum extract of the present invention reduces collagen production of human dermal fibroblasts. Significantly promotes, and simple lingzhi extract is not effective Excellent effects were observed even at a concentration of only 1 μg / ml.

EXPERIMENTAL EXAMPLE 2 Glucosaminoglycan Production-Promoting Activity In the test, human skin fibroblasts (derived from a 6-year-old male, passage number 6) were used as test cells, and Eagle's MEM was used as a medium. The amount of glucosaminoglycan produced was measured using 3H-glucosamine incorporation in glucosaminoglycan as an index. Human skin fibroblasts were seeded at 25,000 cells / well in a 96-well plate (Falcon 3072), and cultured in Eagle's MEM containing 10% FCS at 37 ° C. and 5% CO 2 for 4 days. During this time, the cells became confluent.
Then, just ganoderma extract, polysaccharide fraction ganoderma extract, the onset
Ming reishi extract (Example-1) at a final concentration of 1-10 μg each
/ ml of serum-free Eagle's MEM (50 μg / ml)
ml L-ascorbic acid, containing 18.5KBq 3H-glucosamine)
And cultured for 24 hours. Thereafter, a protease solution was added to each well to degrade proteins in the cells and in the culture supernatant. Next, cetylpyridinium chloride was added to precipitate glucosaminoglycan, which was collected on a glass fiber filter paper. After washing the glucosaminoglycan, the incorporated radioactivity was measured using a liquid scintillation counter (Be
ckman LS 5801). Each sample was measured for 5 wells, and the amount of production was shown as an average value ± standard deviation. The sample without addition was used as a control.

[0024]

[Table 2] Table 2 Glucosaminoglycan (GAG) production promoting action・ ・ Concentration Collagen production Amount of production promotion Significant difference [μg / ml] [DPM / 25,000 cells] [%] ........... control 2400.8 ± 143.6 100 merely ganoderma extract 1 2104.4 ± 493.0 88 10 3086.4 ± 447.3 129 * ganoderma extract 1 2738.4 ± 508.1 114 (polysaccharide fraction) 10 2342.5 ± 189.7 98 Reishi extract of the present invention 1 2644.2 ± 241.9 110 10 3065.7 ± 323.5 128 ** …… Significant difference (vs control) *: P <0.05, **: P <0.005 As shown in Table 2, the Ganoderma lucidum extract of the present invention was significantly different in glucosaminoglycan production. Facilitate.

EXPERIMENTAL EXAMPLE 3 Skin Conditioning Using the lotion of Example-3, the emulsion of Example-5, the conventional lotion of Comparative Example-1 and the conventional emulsion of Comparative Example-2,
15 women suffering from fine lines and sagging skin (20-35 years old)
Was subjected to a one-month use test. After use, the skin conditioning effect (the effect of improving skin abrasion and fine wrinkles) was determined. As a result, as shown in Table 3, the cosmetic containing the reishi extract of the present invention exhibited an excellent skin conditioning effect.

[0026]

[Table 3] Table 3 Skin conditioning ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ Judgment of the number of people ・ ・ ・ ・ ・ ・ ・... Example 3 Comparative Example-1 Example-5 Comparative Example-2・ ・ ・ ・ ・ ・ ・ ・ ・ Very improved 5 3 7 4 Improved 5 4 4 3 Not much improved 3 4 3 5 Not improved 2 4 1 3 ...

The cream of Example-4, the pack of Example-6, the foundation of Example-7, and the bath preparation of Example-8 were similarly subjected to use tests, and showed excellent skin conditioning. . As described above, the Ganoderma lucidum extract of the present invention has an excellent collagen production promoting action and a glucosaminoglycan production promoting action, and further, a cosmetic characterized by containing the extract is excellent. Showed a skin conditioning effect. In addition, a comparison was made of the coloring properties when a simple ganoderma extract and the ganoderma extract of the present invention were respectively added to cosmetics (Example-5 and Comparative Example-2). Improvement was also observed.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-58-113118 (JP, A) JP-A-63-208507 (JP, A) JP-A-1-42412 (JP, A) JP-A-4- 9321 (JP, A) (58) Field surveyed (Int. Cl. ⁷ , DB name) A61K ^7/ 00-7/50 CA (STN)

Claims (1)

(57) [Claims] [1] The fruiting body of Ganoderma lucidum was extracted with a water-soluble solvent.
Later, the polysaccharide is precipitated by adding more alcohol.
A cosmetic comprising a ganoderma extract extracted and removed .