JP2003342156A - External preparation for skin for prevention of aging - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an external preparation for the skin for prevention of aging comprising an extract of Selenicereus. <P>SOLUTION: The external preparation for the skin for prevention of aging comprises an extract of Selenicereus. The extract of Selenicereus exhibits excellent cleavage-promoting effect of keratinocyte and ATP production-promoting effect. The external preparation for the skin comprising the extract of Selenicereus exhibits aging-preventing effect of the skin. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin external preparation, and more particularly to a skin external preparation that exhibits its effectiveness in preventing skin aging.

[0002]

2. Description of the Related Art The epidermis is constantly undergoing metabolism throughout the keratinocyte basal layer division and subsequent differentiation processes. Keratinocyte division is the source of epidermal metabolism, or turnover, but it has been reported that there is a delay in turnover rate with aging (Roberts, D., J. Invest.
Dermatol. , 1980, 74, 13-16).
Delayed epidermal turnover rate associated with decreased keratinocyte mitogenicity is thought to cause thinning of the epidermis and thickening of the stratum corneum, resulting in dryness, fine wrinkles, dullness, and drooling in aged skin. . As a means for suppressing such aging of the skin, a skin external preparation for applying a substance lost to aging such as collagen, elastin, hyaluronic acid and the like to the skin has been the mainstream. However, these did not have sufficient effects.

[0003]

DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention As described above, the delay of the epidermal turnover rate due to the decrease in keratinocyte division ability is considered to be one of the causes of skin aging. It is thought that promotion promotes suppression of skin aging. EGF, estrogen, and the like are known as substances that promote keratinocyte division, but these substances have problems in stability and their effect is not sufficient, and a stable and highly effective keratinocyte mitogenic effect is known. There is a need for an external preparation for skin having Furthermore, in order to promote cell division, it is important to supply the cells with the energy required for division. ATP (adenosine 5'-triphosphate) is raised as the most important substance of energy metabolism of the living body, and plays an important role in energy acquisition and utilization. It is considered that increasing the production amount of this ATP promotes intracellular energy metabolism and leads to cell growth. Conventionally, as a substance having an ATP production promoting effect, vegetable oil (Japanese Patent Laid-Open No. 6-2199926) has been reported, but a substance having a higher effect is required.

[0004]

Under these circumstances, the inventors of the present invention have conducted extensive studies and as a result, have found that the extract of Tsukiboshi Bijin is stable and has an excellent keratinocyte mitogenic effect and AT.
The present invention has been completed by discovering that it has a P production promoting effect and further that an external preparation for skin containing an extract of Bijinshi Bijin has an excellent antiaging effect.

That is, the present invention is an external anti-aging agent for skin, which contains an extract of a beautiful woman under the skin.

[0006] The term "Beauty of the Moon" used in the present invention means the genus of peafactose cactus (Epiphyllum o).
xypetalum Haw. ) And its relatives, and it is possible to use dried plants of whole plants and flowers of the horticultural variety.

The extraction site is not particularly limited, and any site can be used, and flowers are preferable. The extraction method is not particularly limited, and may be, for example, heat extraction or room temperature extraction.

The solvent to be extracted is, for example, water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohol (1,3-butylene glycol). , Propylene glycol, glycerin, etc.), ketones (acetone, methyl ethyl ketone etc.), acetonitrile, esters (ethyl acetate, butyl acetate etc.), hydrocarbons (hexane, heptane etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether) etc)
And so on. A polar solvent such as water or lower alcohol is preferable. These solvents may be used alone or in combination of two or more.

The above extract may be used as it is as an extracted solution, and if necessary, it may be subjected to treatments such as concentration, dilution, filtration, decolorization with activated carbon, deodorization, ethanol precipitation and the like. Furthermore, the extracted solution is concentrated to dryness, spray-dried,
It may be used as a dried product after being subjected to a treatment such as freeze-drying.

In the external preparation for skin of the present invention, the extract of the above-mentioned menstrual beauty may be used as it is, and as long as the effect is not impaired, it is a component used in ordinary cosmetics, quasi drugs and pharmaceuticals. Certain fats, waxes, hydrocarbons, fatty acids,
Alcohols, esters, surfactants, metal soaps, p
H conditioner, antiseptic, fragrance, moisturizer, powder, ultraviolet absorber, thickener, pigment, antioxidant, whitening agent, chelating agent,
Ingredients such as an excipient, a stabilizer, a preservative, a binder, a disintegrant, etc. can also be added.

The external preparation for skin of the present invention can be used in any of cosmetics, quasi-drugs, and pharmaceuticals. Examples of its dosage form include lotion, cream, emulsion, gel, aerosol, and poultice. Agent, essence, pack, cleaning agent,
Examples include bath agents, foundations, dusting powders, lipsticks, ointments and the like.

[0012] When the extract of the beauty of the moonlight used in the present invention is blended with the external preparation for skin, the blending amount is not particularly limited, but it is preferably in the range of 0.0001 to 30% by weight as a dried product, more preferably 0. It is 0.001 to 10% by weight. If it is 0.0001% by weight or less, the keratinocyte's mitogenic effect and ATP production promoting effect are low, and if it exceeds 30% by weight, the effect is not significantly enhanced and it is not efficient. Regarding the method of addition, it may be added in advance or may be added during the production, and may be appropriately selected in consideration of workability.

In order to describe the present invention in detail, production examples, formulation examples and experimental examples of the extract used in the present invention will be given as examples, but the present invention is not limited thereto. The compounding amounts shown in the examples are% by weight.

Production Example 1 Tsukishita Bijin Extract 1 To 100 g of dried Tsukishita Bijin's flower, 3 L of purified water was added.
Extraction was performed at 5 to 100 ° C for 2 hours. After filtration, the filtrate was concentrated under reduced pressure, and further freeze-dried to obtain 48 g of the extract of Tsukijoshi Bijin.

Production Example 2 Tsukishita Bijin Extract 2 3 L of purified water was added to 100 g of dried dried stalks of Tsukishita Bijin,
Extracted at 95-100 ° C for 2 hours. After filtration, the filtrate was concentrated under reduced pressure, and further freeze-dried to obtain 11 g of the Tsukihabijin extract.

Production Example 3 Tsukishita Bijin Extract 3 To 100 g of dried Tsukishita Bijin leaf, 3 L of purified water was added to give 9
Extraction was performed at 5 to 100 ° C for 2 hours. After filtration, the filtrate was concentrated under reduced pressure and further freeze-dried to obtain 16 g of the extract of Tsukijoshi Bijin.

Production Example 4 Tsukishita-Bijin Extract 4 To 100 g of dried above-ground whole grass of a Tsukishita-Bijin, 1 L of a 30% ethanol aqueous solution was added, and the mixture was extracted at room temperature for 1 week. After filtration, the filtrate was concentrated under reduced pressure, and further freeze-dried to obtain 9 g of the extract of Tsukijoshi Bijin.

Example 1 Cream Formulation Amount (wt%) 1. Geisha Bijin Extract 1 (Production Example 1) 0.05 2. Squalane 5.5 3. Olive oil 3.0 4. Stearic acid 2.0 5. Beeswax 2.0 6. Octyldodecyl myristate 3.5 7. Polyoxyethylene cetyl ether (20 EO) 3.0 8. Behenyl alcohol 1.5 9. Glycerin monostearate 2.5 10. Fragrance 0.1 11.1, 3-butylene glycol 8.5 12. Ethyl paraoxybenzoate 0.05 13. Methyl paraoxybenzoate 0.2 14. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 9 are heated and mixed, and maintained at 70 ° C to form an oil phase. Ingredients 1 and 11 to 14 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, the mixture is cooled with stirring, component 10 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Comparative Example 1 A conventional cream was prepared by replacing Gesugebijin Extract 1 in the conventional cream Example 1 with purified water.

Example 2 Cosmetic lotion formulation Amount (% by weight) 1. Geisha Bijin Extract 2 (Production Example 2) 0.01 2.1,3-butylene glycol 8.0 3. Glycerin 2.0 4. Xanthan gum 0.02 5. Citric acid 0.01 6. Sodium citrate 0.1 7. Ethanol 5.0 8. Methyl paraoxybenzoate 0.1 9. Polyoxyethylene hydrogenated castor oil (40 EO) 0.1 10. Perfume proper amount 11. [Production method] Components 1 to 6 and 11 and components 7 to 10 each having a total amount of 100 with purified water are uniformly dissolved, and both are mixed and filtered to obtain a product.

Example 3 Emulsion formulation Compounding amount (wt%) 1. Geisha Bijin Extract 3 (Production Example 3) 0.05 2. Squalane 5.0 3. Olive oil 5.0 4. Jojoba oil 5.0 5. Cetanol 1.5 6. Glycerin monostearate 2.0 7. Polyoxyethylene cetyl ether (20 EO) 3.0 8. Polyoxyethylene sorbitan monooleate 2.0 9. Perfume 0.1 10. Propylene glycol 1.0 11. Glycerin 2.0 12. Methyl paraoxybenzoate 0.2 13. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 8 are dissolved by heating and mixed, and the temperature is 70 ° C.
Keep it in the oil phase. Ingredients 1 and 10 to 13 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The water phase is added to the oil phase to emulsify, and the mixture is cooled with stirring, component 9 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Example 4 Ointment Formulation Compounding amount (wt%) 1. Geisha Bijin Extract 4 (Production Example 4) 1.0 2. Polyoxyethylene cetyl ether (30EO) 2.0 3. Glycerin monostearate 10.0 4. Liquid paraffin 5.0 5. Cetanol 6.0 6. Methyl paraoxybenzoate 0.1 7. Propylene glycol 10.0 8. [Manufacturing method] The total amount is 100 with purified water. Components 2 to 5 are dissolved by heating and mixed, and the temperature is 70 ° C
Keep it in the oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and the mixture is kept at 75 ° C to form an aqueous phase. The water phase is added to the oil phase to emulsify, and the product is cooled to 30 ° C. with stirring.

Example 5 Foundation Formulation Amount (wt%) 1. Geisha Bijin Extract 1 (Production Example 1) 0.25 2. Talc 27.7 3. Sericite 30.0 4. Titanium oxide 10.0 5. Fine particle titanium oxide 5.0 6. Spherical silica 10.0 7. Red iron oxide 0.4 8. Yellow iron oxide 1.4 9. Ethyl paraoxybenzoate 0.3 10. Methyl polysiloxane 14.6 11. Vitamin E 0.3 12. Fragrance 0.05 [Production method] Components 1 to 10 are uniformly mixed, and then components 11 and 12 are added and mixed.

[0024]   Example 6 Bath agent Prescription compounded amount (% by weight)   1. Geisha Bijin Extract 2 (Production Example 2) 5.0   2. Sodium hydrogen carbonate 50.0   3. Yellow No. 202 (1) Suitable amount   4. Fragrance suitable amount   5. Adjust the total amount to 100 with anhydrous sodium sulfate [Manufacturing method] Components 1 to 5 are uniformly mixed to obtain a product.

Next, in order to explain the effects of the present invention in detail, experimental examples will be given.

Experimental Example 1 Keratinocyte mitogenesis test The mitogenic effect of cultured keratinocytes was measured under the following conditions.

[0027] Pam212 cells derived from mouse keratinocytes were seeded in 30,000 cells in a 35 mm dish, and the sample was added so that the final concentration was 10 µg / mL.
The cells were cultured in Eagle's MEM culture medium containing 5% fetal bovine serum at 37 ° C. under 5% CO ₂ for 6 days. As a control, culture was performed without adding a sample. Then 0.2
The cells were detached with a 5% trypsin solution, and the number of cells was measured using a hemocytometer. The keratinocyte mitogenic rate was calculated from the ratio of the number of cells when the sample was added to the number of control cells.

The results of these tests are shown in Table 1. As a result, an excellent keratinocyte mitogenic effect was observed in the extract of Geisha Bijin. In particular, the strong mitogenic effect was observed in the extract of the flower of the beauty of the moonlight of the beauty of the moonlight 1 (Production Example 1).

[0029]

[Table 1]

Experimental Example 2 ATP production promoting test The promoting effect of ATP production in cultured keratinocytes was measured under the following conditions.

Eagle's M containing 0.5% fetal bovine serum supplemented with 10 μg / mL of Pam212 cells derived from mouse keratinocytes in a confluent state.
The cells were further cultured in EM medium for 24 hours. The control is
The culture was performed without adding the sample. Then, the cells were detached with a 0.25% trypsin solution, and the number of cells was measured using a hemocytometer. In addition, the intracellular ATP content is
TP measurement kit (Lucifer 250, Kikkoman)
Was measured using. That is, ATP of luciferase
Fluorescence emission was measured by a luminometer. The ATP content per cell was calculated from the measured cell number and ATP amount, and the ATP production promoting rate was determined from the ratio of the ATP content at the time of sample addition to the control ATP content.

The results of these tests are shown in Table 2. As a result, an excellent ATP production-promoting effect was observed in the extract of Geisha Bijin. In particular, a significantly higher ATP production-promoting effect was observed in the extract from the flower of the beauty of the moon under the beauty of the moonlight 1 (Production Example 1).

[0033]

[Table 2]

Experimental Example 3 Usage Test Using the cream of Example 1 and the conventional cream of Comparative Example 1, 30 women (30 to 45 years old) were subjected to a usage test for 2 months. After use, a questionnaire survey was conducted on the improvement of texture, wrinkles and dullness, and the antiaging effect was judged. The evaluation criteria of the questionnaire were evaluated as "excellent" for effective, "good" for slightly effective, "acceptable" for slightly effective, and "impossible" for ineffective.

The results are shown in Table 3. The external preparation for skin, which was characterized by containing the extract of Bijinshibi 1 of Example 1, showed an excellent anti-aging effect. There was no skin trouble during the test period, and there was no problem in safety.

[0036]

[Table 3]

Toner lotion of Example 2, emulsion of Example 3, ointment of Example 4, foundation of Example 5 and Example 6
When the use test of these bath agents was conducted, all showed safe and excellent antiaging effect.

[0038]

EFFECTS OF THE INVENTION From the above, the extract of the present invention is excellent in keratinocyte mitogenic effect and ATP.
The production-promoting effect was shown. The external preparation for skin, which is characterized by containing the extract of Bijinshi Bijin, showed a safe and excellent anti-aging effect on the skin.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Hirose Osamu             2-7 Torimicho, Nishi-ku, Nagoya-shi Japan Menard             Cosmetics Research Institute (72) Inventor Tomonori Katata             2-7 Torimicho, Nishi-ku, Nagoya-shi Japan Menard             Cosmetics Research Institute F-term (reference) 4C083 AA082 AA111 AA112 AA122                       AB172 AB232 AB242 AB312                       AB352 AB432 AB442 AC022                       AC072 AC122 AC182 AC242                       AC302 AC352 AC422 AC432                       AC482 AC842 AD152 AD352                       AD662 CC04 CC05 DD31                       EE06 EE12                 4C088 AB12 AC03 BA08 MA63 NA14                       ZA89 ZB21

Claims (4)

[Claims] 1. An external anti-aging agent for skin, which contains an extract of Bijinshi Bijin. 2. A keratinocyte mitogen, which contains an extract of Bijinshi Bijin. 3. A keratinocyte ATP production promoter, which comprises an extract of Tsukiboshi Bijin. 4. The anti-aging skin external preparation, the keratinocyte mitogen or the ATP production promoter according to claims 1 to 3, wherein the extract of Bijinshiki Bijin is an extract obtained from flowers.