KR20050104934A - Cosmetic composition of lifting and whitening ingredient containing saponin extracted from rhynchosia molubilis - Google Patents

Abstract

The present invention relates to a functional cosmetic composition containing medicinal soybean saponin as an active ingredient. Specifically, saponins extracted from medicinal soybeans have anti-wrinkle effect and tyrosinase inhibition through antioxidant action such as UV protection, automatic oxidation prevention, and free radical scavenging. It has excellent whitening effect through melanin activity inhibitory action such as melanin production inhibition and can be used as cosmetic raw material with excellent functionality and excellent safety.

Description

Cosmetic composition for wrinkle improvement and whitening containing saponin derived from medicinal beans {Cosmetic Composition of Lifting and Whitening Ingredient Containing Saponin extracted from Rhynchosia Molubilis}

The present invention relates to a functional cosmetic composition containing weak bean saponin as an active ingredient.

More specifically, the saponin extracted from the medicinal soybean according to the present invention has a whitening effect through anti-wrinkle effect and anti-melanin activity, such as anti-wrinkle effect, anti-oxidation, free radical scavenging, anti-wrinkle effect, tyrosinase inhibition, melanin production inhibition It can be used as a cosmetic raw material having excellent functionality and excellent safety.

Medicinal beans ( Rhynchosia Molubilis ) is a Korean native black bean (black bean) that grows in Jeongseon province, Gangwon-do. It is named after its ancestors, and its shape and color are small, its color is black, and it shines like a rat's eye. It is also called a rat eye bean in a sense, and it is also called Seomoktae by translating it into Chinese. Medicinal beans are distinguished from common common white tigers, which are black in color but large in size, and are clearly distinguished from Glycine max Merrill, which is native to Manchuria, China.

Medicinal beans are annual herbaceous leaves, with three leaves, with alternate leaves. Flowers bloom in the axilla in mid-August, and weigh 100 seeds (12.47g). Growth characteristics are similar to that of normal black beans, but the leaves are small and the pods contain about 2 seeds, sowing in mid and late May and harvesting in early October.

Medicinal beans contain 38.5% carbohydrates, 34.7% crude protein, and 15.9% crude fat.Inorganic ingredients are rich in potassium, phosphorus, calcium and magnesium, and glucose and galactose in sugars. Linoleic acid, oleic acid, and linolenic acid are the predominant species, with 82.63% of them being especially high in linoleic acid. The content of isoflavin, one of the phytoestrogens known as phytoestrogens, is twice as high as that of soybean, and the content of phenolic compound, an antioxidant active substance, is high in the soybean peel. Although soybeans and soybeans are similar in general nutrition, there are clear differences in functional ingredients, mainly isoflavin and phenolic compounds.

Functional cosmetics have been rapidly developed by the introduction of functional cosmetics in the Cosmetics Act, which was enacted in 1999 from the Pharmacist Act in 1999 and its enforcement was confirmed from July 1, 2000. The 21st century is nearing the era of functional cosmetics. Functional cosmetics prescribed by Ordinance of the Ministry of Health and Welfare are products that help to whiten the skin, products that help to improve the wrinkles of the skin, and products that burn the skin finely or protect the skin from ultraviolet rays. Among the benefits and effects required in cosmetics, whitening is one of the most important benefits and effects along with wrinkle prevention, hair growth and wool. Pigmentation of the skin is caused by melanin produced by the pigment cells in the basal layer of the epidermis, and agents that inhibit melanin production have been actively studied as an effective raw material for whitening cosmetics. On the other hand, natural products have recently attracted attention as a raw material supply source for cosmetics or quasi-drug products in the cosmetic field. In addition, research on the whitening effect of plant-derived ingredients is being actively conducted.

The research on medicinal soybeans as a functional natural product is concentrated in the food, beverage, and pharmaceutical fields, such as the anticancer and antioxidant effects of isoflavin. Therefore, the research to use as a raw material of cosmetics is still insufficient.

In addition, saponins, which are one of the most popular functional substances, are mainly used saponins separated from ginseng, but since ginseng saponins are expensive raw materials, when applied to functional products, the cost of products increases the product's economic profitability. It acts as a factor. Therefore, in order to overcome these difficulties, development of new saponins of low cost and high quality as substitutes for ginseng saponins has been required.

The present inventors have focused on saponins, which are functional raw materials, to develop new separation sources of saponins which are cheaper than ginseng and are not inferior in quality, and are intended to be applied to functional cosmetics having anti-aging and whitening functions.

It is an object of the present invention to provide a new separation source of saponin, a functional substance, and to provide the saponin obtained through this as a raw material for cosmetics for wrinkle improvement and whitening.

The present invention provides a functional cosmetic containing saponin derived from medicinal beans. Functionality at this time includes wrinkle improvement or whitening activity.

Hereinafter, the present invention will be described in detail.

The present invention provides a functional cosmetic composition containing saponin extracted from medicinal beans as an active ingredient.

To this end, in the present invention, saponin components were extracted from the medicinal beans. Specifically, medicinal soybeans were sequentially extracted using aqueous and organic solvents to obtain purified saponins. Methods for purifying saponin from medicinal soybeans can be any of conventional extraction methods known to those skilled in the art.

In order to verify whether the medicated soybean saponin can be used as a raw material of functional cosmetics, the present invention searched for the biological activity of the soybean saponin.

First of all, free radical scavenging activity, automatic antioxidant activity, and ultraviolet ray absorption effect were tested to confirm the effect of wrinkle improvement.

The process of skin aging by free radicals includes lipid peroxidation, protein oxidation, DNA oxidation, melanin production, connective tissue breakdown and abnormal crosslinking. Most of these reactions are radical reactions and proceed through automatic oxidation. Automated oxidation is a chain reaction, which causes many biological components to undergo oxidative damage during this process. Therefore, the automatic oxidation reaction should be blocked as soon as possible. Antioxidants are responsible for blocking the automatic oxidation in vivo.

For example, the most representative lipid peroxidation reaction of the automatic oxidation reaction, phospholipids, which are components of the cell membrane, contain many polyunsaturated fatty acids. Free radicals produced in the skin, such as by exposure to ultraviolet rays, generate lipid radicals by deodorizing allylzyl hydrogen from reactive methylene groups in unsaturated fatty acids, and then react with oxygen after electron rearrangement to generate peroxide radicals. The resulting peroxide radicals create new lipid radicals, which trigger a chain reaction that produces peroxide radicals. The chain reaction of this process is called automatic oxidation reaction. Termination of this automatic oxidation reaction is accomplished by an antioxidant such as tocopherol giving hydrogen atoms to the peroxide radical.

The ability to give a hydrogen atom, which is the reducing power, the greater the reducing power is a powerful antioxidant. A reagent capable of measuring such reducing power is 1,1-diphenyl-2-picryl-hydrazyl radical (DPPH). This DPPH method is very simple and can be used in a short time. Therefore, the DPPH method can be used when searching for antioxidants in natural products or when evaluating anti-aging cosmetic ingredients.

Specifically, in the present invention, the free radical scavenging action of the saponin derived from the soybean according to the present invention was confirmed by the DPPH method, and the autooxidative inhibitory activity was tested using linoleic acid, which is a good autooxidant.

As a result, the weak soybean-derived saponin according to the present invention was shown to have excellent free radical scavenging activity, and also had an excellent antioxidant effect (see FIGS. 1 and 2).

Solar ultraviolet rays also have a significant effect on skin pigmentation. When the skin is exposed to solar ultraviolet light, which is roughly classified as UVB (290-320 nm) and UVA (320-400 nm), UVB mainly reaches the epidermal layer of the skin and UVA reaches the dermal layer.

UVB causes inflammation or is involved in cell damage, and also causes or participates in pigmentation. Specifically, UVB causes keratinocytes, which are epidermal cells, to release melanin synthesis command factors, thereby inducing melanin synthesis by activating melanocytes.

UVA, on the other hand, reaches the dermis and damages the dermis via the production of free radicals. In particular, it is reported to promote wrinkle formation by destroying proteins such as collagen and glycosaminoglycan, which are connective tissues of the dermis. Therefore, it is necessary to block UVB and UVA in order to suppress pigmentation and wrinkle formation from ultraviolet rays.

In the present invention, in order to confirm the degree of ultraviolet absorption of the medicinal soybean-derived saponins according to the present invention, arbutin and ultraviolet absorbance known to have excellent UV blocking ability were measured and compared.

As a result, the weak soybean-derived saponins according to the present invention showed ultraviolet absorbance similar to that of arbutin (see FIG. 3).

Therefore, the weak soybean-derived saponin according to the present invention has been found to be useful for anti-wrinkle because of its excellent antioxidant effect, free radical scavenging activity and UV blocking effect.

In addition, the anti-wrinkle effect of the weak soybean-derived saponin according to the present invention was confirmed through an in vivo test. That is, wrinkles are easily generated by the increase of UV rays or age, and thus anti-wrinkle and improvement effects were evaluated by using white rabbits that cause wrinkles with UV rays.

As a result, it was confirmed that the weak soybean-derived saponin according to the present invention has a remarkable effect on wrinkle improvement with the naked eye and feel (see FIG. 4).

Biological Activity Confirmation Test of Medicinal Soybean-Derived Saponins According to the Present Invention In order to confirm the whitening effect, the tyrosinase activity inhibition was tested and the degree of whitening was visually observed.

Melanin produced by human pigment cells is roughly divided into eumelanin and pheomelanin. The production process of eumelanin is as follows. That is, tyrosine is oxidized to dopaquinone (DOPA-quinone) via dopa (DOPA, 3,4-dihydroxyphenylalanine) by tyrosinase, and dopaquinone spontaneously forms an endocycle to become leucodopachrome. . Rucodopachrome is oxidized in a reductive compatibility with dopaquinone to form dopa and dopaquinone. Dopa is again oxidized by tyrosinase to form dopaquinone, and the dopa / dopaquinone redox circuit acts as an oxidative driver of melanin production. Dopachrome is then converted to 5,6-dihydroxy-indol (DHI) and 5,6-dihydroxyindol carboxylic acid (DHICA) by CO ₂ desorption and dopachome tautomerase. The intermediate metabolite is polymerized by autooxidation reaction, mineralization reaction or tyrosinase, dopachrome tautomerase, DHICA-oxidase and the like to become eumelanin. Automatic oxidation is facilitated by metal ions, especially divalent ions such as iron and copper. Pheomelanin, on the other hand, is produced via adducts such as cystenyl dopa to dopaquinone.

In the 1990s, the study of melanin production using molecular biological techniques was greatly developed. Along with this, a new development is being made from the one-enzyme theory of the melanin production mechanism to the three-enzyme theory. The application of the three-enzyme theory related to melanin production is becoming a new approach for the development of whitening agents. However, as described above, the melanin production process is largely divided, and it can be seen that the enzymatic action and automatic oxidation reaction of tyrosinase, such as a key enzyme. A critically important key enzyme in melanogenesis is tyrosinase. The enzyme is involved in catalyzing the conversion of tyrosine to dopa, dopa to dopaquinone, and DHI to eumelanin during the melanin biosynthesis process starting with the amino acid tyrosine.

      Advances in melanin production and control mechanism research in recent years have been very significant (Mishima, Y., et al., 1998; Maeda, K., 1997; Mishima, Y., et al., 1996). As a result of this research, problems of whitening mechanisms such as inhibition of tyrosinase in pigment cells, inhibition of melanin production, inhibition of tyrosinase activity, and promotion of melanin release have been studied in various ways. In particular, the presence of arachidonic acid metabolites such as prostaglandin E2, pigment cell hormone, and the like has been shown to be involved in melanin production by ultraviolet light (Maeda, K., 1997; Mishima, Y., et al., 1996). In addition, a method of inhibiting melanogenesis that traps melanin monomers with appropriate compounds in melanocytes has been proposed (Mishima, Y., et al., 1998). As a conventional whitening agent, vitamin C derivatives (Egawa, M., et al. , 1997). Arbutin and kojic acid (Maeda, K., et al., 1991) are licensed as active ingredients. Recently researched and developed as a whitening agent, DL-α-tocopheryl ferulate (Funasaka, Y., et al, 1997). Stable vitamin C derivatives (eg L-ascobic acid-2-glucoside; Egawa, M, et al., 1997), ellagic acid (Tachibana, S., et al., 1997), α-hydroxy acids (AHAs) (Sinomiya, T., 1997), 3,5-dicaffeoy 1-quinate (Shinmotom, H., 1997), and other reports on synergistic whitening by the combination of kojic acid and glabridin, an oil-soluble licorice extract. (Koide, G., et al., 1997).

In the present invention, whitening using a method of inhibiting the function of tyrosinase and measuring the extent to which tyrosine is inhibited from oxidizing to form a black polymer called melanin (Pomerantz SH, J. Biochem., 24, 161-168 (1966)) The effect was judged.

As a result, the weak soybean-derived saponin according to the present invention was found to have higher activity inhibitory effect compared to vitamin C, which is known to have excellent whitening effect, and even when the whitening effect was observed visually by applying the soybean-derived saponin to experimental animals and volunteers. The whitening effect was found to be excellent (see Table 2, Figure 5).

Therefore, the weak soybean-derived saponin according to the present invention can be used as an excellent whitening cosmetic.

The present invention provides a cosmetic composition excellent in safety. To this end, the cumulative skin irritation of the medicinal soybean-derived saponins according to the present invention was tested and the toxicity by light was tested.

Specifically, the cumulative skin irritation was tested by examining the irritation that appears locally after continuous contact with the skin using a test animal for the possibility of accidental or intentional contact with human skin among chemicals.

In addition, UV light was irradiated daily through the skin irritation response indicated by light to test the presence of phototoxicity, and then the presence of phototoxicity was determined by evaluation criteria.

As a result, it was confirmed that the weak soybean-derived saponin of the present invention showed little skin cumulative irritation or phototoxicity.

The medicated soybean saponin extracted according to the present invention can be used as a raw material for cosmetics, and for this purpose, a cosmetic composition is prepared by adding a conventional excipient. Specifically, the cosmetic composition of the present invention may be in the form of an aqueous solution, an oil liquid, or other solutions such as emulsions, creams, gel suspensions, microcapsules, powders, granules, capsules, solids, and the like. They are formulated in their original form by known methods, or prepared as lotions, emulsions, creams, ointments, warnings, paps, aerosols, injections, oral preparations, suppositories, etc. You can inject, drink or insert. Among these formulation forms, external skin preparations such as lotions, emulsions, creams, ointments, warnings, patches, aerosols and the like are preferred.

The composition of the present invention includes oils, surfactants, preservatives, metal ion sequestrants, water-soluble polymers, thickeners, powder ingredients, sunscreens, moisturizers, active ingredients, antioxidants, pH adjusters, cleaning agents, as well as general-purpose excipients and fragrances. , Desiccant, emulsifier and the like may be appropriately included. When adding these various components to the composition of this invention, it should add in the range which does not impair the intended objective of this invention.

The present invention will be described in more detail with reference to the following Examples, but the scope of the present invention is not limited to these Examples, and various modifications and variations are possible within the protection scope of the present invention described in the claims. It will be apparent to those of ordinary skill in the art.

Example 1 Extraction of Weak Bean Saponin

100 g of weak beans and 500 ml of 70% alcohol were refluxed for 3 hours. The filtrate was concentrated, and 120 ml of ether and 200 ml of distilled water were added to a separatory funnel in 40 ml of the concentrate, followed by phase separation for 4 hours. Then, 120 ml of butanol was added to the lower aqueous layer, followed by phase separation for 4 hours. The butanol layer was washed five times with 100 ml of distilled water, and the washed butanol layer was concentrated and distilled water was added to completely remove butanol.

The concentrated solution was freeze-dried to extract 0.5 g of saponin (yield 0.5%).

Example 2 Formulation of Medicinal Soybean Saponin Cream

Weak bean saponin cream was prepared according to the prescription of Table 2 below.

Composition of medicinal soy saponin cream ingredient Control Cream (%) Akkong Saponin Cream (%) Hydroxy ethyl cellulose 0.1 0.1 Propylene glycol 10 10 antiseptic 0.4 0.4 Carboxy Vinyl Polymer 0.2 0.2 Glyceryl Stearate 3.5 3.5 Monostearic acid polyoxyethylene sorbitan 0.5 0.5 Cetostearyl alcohol 2.5 2.5 Tocopherol Acetate 0.5 0.5 Olibuyu 4.0 4.0 Floating paraffin 7.0 7.0 Triethanolamine 0.3 0.3 Medicinal Bean Saponins No addition 0.3 addition Purified water Add appropriate amount to make it 100 weight%.

<Test Example 1> Free radical scavenging action

In 0.5 ml of DPPH solution, 1 ml of sample (weak soy saponin, weak soy saponin cream and control cream according to Example 2) at a concentration of 0.23%, 0.3%, 0.6%, 0.9%, 1.2%, 1.5%, 1.8%, 2.1% After adding 1ml of ethanol solution, the mixture was mixed and left for 10 minutes at 37 ° C. The absorbance was measured at 517 nm, and the results are shown in FIG. 1.

As shown in FIG. 1, compared to the negative control cream, the soybean saponin cream showed a significant free radical scavenging effect, and the purified soybean saponin showed similar activity as that of the positive control vitamin C.

<Test Example 2> linoleic acid automatic oxidation prevention activity

3 ml of linoleic acid solution and 0.075 ml of each sample adjusted to 4 mg / ml were added and then left at 4 ° C. for 24 hours. After the mixture was added to ethanol and stirred, 0.1 ml of ammonium thiocyanate solution and 0.1 ml of ferrous chloride were added thereto, and the mixture was left for 3 minutes, and then the absorption wavelength was measured at 500 nm.

As shown in Figure 2, compared to the control cream, medicinal soybean saponin, medicinal soybean saponin cream was shown to have an antioxidant effect similar to vitamin C and arbutin excellent in the antioxidant effect.

Test Example 3 UV Absorption Spectrum Measurement

Ultraviolet absorbance (190 ~ 350nm) was measured by comparing the solvent dissolved in each sample using an ultraviolet spectrometer.

As shown in FIG. 3, the medicinal soybean saponin and the medicinal soybean saponin cream according to the present invention showed an effect similar to that of arbutin, which is known to have excellent UV blocking ability.

<Test Example 4> Wrinkle improvement measurement of rabbit skin

Wrinkles easily occur on the skin due to the increase of UV rays and age, so 500ml of each sample is applied twice a day using white rabbits (1.5 ~ 3.0 kg in weight) that caused wrinkles by UV to prevent wrinkles and improve efficacy. Evaluated.

As shown in FIG. 4, the rabbit skin before the application was measured by the naked eye and the texture of the skin was wrinkled and the texture was beaten.

<Test Example 5> Tyrosinase activity inhibition test

For inhibitory activity against tyrosinase, 15 µl of each sample was placed in a microplate (96well), 150 µl of phosphate buffer solution (PH 6.86) and 25 µl of L-tyrosine solution were added, followed by mushroom tyrosinase (sigma, USA). After adding 7 μl and reacting at 30 ° C. for 10 minutes, the resultant was measured at 490 nm using a microplate.

Tyrosinase activity inhibitory effect IC ₅₀ Weak bean saponin 0.05% Pharmacy saponin cream 0.1% Control cream One% Arbutin 0.0005% Vitamin C 0.0005%

IC ₅₀ is the concentration of inhibitor reaching 50% inhibition rate of enzyme activity.

When comparing the sample material with arbutin and vitamin C, which showed excellent whitening effect, medicinal soybean saponin cream had higher inhibitory effect on tyrosinase enzyme activity than that of control cream. Was excellent.

<Test Example 6> rabbit skin whitening experiment

Melanin was applied to the skin of white rabbits (weight 1.5-3.0 kg) to produce black spots, and then the whitening effect was evaluated by removing melanin 20 days after applying 500 mg of each sample daily.

As shown in FIG. 5, the control cream showed a similar change to the control group that was left unattended, but the dark soybean saponin and the weak soy saponin cream disappeared significantly.

Test Example 7 Observation of Skin Whitening Effect of Human Body

A control cream and a weak soy saponin cream were applied to the face divided into two groups of ten experimenters (20-40 years old female). Each group applied control cream and medicinal soybean saponin cream 500mg twice a day for 1 consecutive month.

A control cream was applied to the left face of one group and the right non-coated, and the other group applied the saponin saponin cream to the left face.

The skin color of the applied areas on both sides of the face was analyzed by image analysis, and the dark color was set to 5, the middle color to 3, and the brightest color to 1.

Human skin whitening effect Experimental number One 2 3 4 5 6 7 8 9 10 Right skin color (no cream part) 3 3 2.5 3 3 3 2.5 3 3 2.5 Left skin color (control cream application) 2.5 2.5 2.5 3 2.5 3 2 2.5 2.5 2 Right skin color (no cream part) 2.5 3 2.5 3 3 3 2.5 3 3 2.5 Left skin color (weak bean saponin cream applied part) 1.5 1.5 One 1.5 1.5 1.5 One One 1.5 One

As can be seen in Table 4, it was confirmed that the whitening effect of the weak soy saponin cream compared to the control cream.

Test Example 8 Cumulative Skin Irritation Test

(1) Breeding environment and management

(A) Breeding environment: Room temperature 23 ± 3 ℃, Humidity 50 ± 10%

(B) Feed and negative water: solid feed for rabbits, negative water for drinking water, free intake

(2) Composition of test group

Three healthy female animals were used, and the material was applied to the test area with the back area of the animal about 2.0 × 2.0 cm in size, with the area around the spine as the control and the remaining area as the test compartment.

(3) Experiment method

(A) Hair removal was performed 24 hours before application of the test substance (3 rabbits), and a test site (each 2.0 × 2.0 cm) was defined.

(B) The wounded area of the spy was wound with a 16G injection needle and left for 24 hours.

(C) The test substance [weak soy saponin, weak soy saponin cream, control cream] was applied to the test compartment 0.5g twice a day and evening for 16 days. The control compartment was left in the air.

(4) observation and inspection items

(A) Observation of general symptoms: daily appearance, feed intake, negative state and general phenomenon after administration of test substance.

(B) Body weight measurement: measured at the time of administration and 16 days of application.

(C) Observation of the applied part: After 30 days, 24 hours, 48 hours, 72 hours after application of the test substance 16 days, erythema, crust formation, edema formation, etc. were respectively confirmed, and the stimulus evaluation scores each time were summed and averaged.

(5) evaluation of skin response and determination of irritation

Evaluation of skin reactions is made using the "toxicity test criteria for pharmaceuticals". In addition, the determination of the degree of stimulus for the result follows the commonly used method of calculating the Primary Irritation Index (P.I.I) of Draize.

P.I.I Evaluation Criteria Table Skin response grade Erythema formation Edema Hongban Fan Jeongpyeong Edema judgment score Have no erythema No edema 0 0 Weak erythema Weak erythema One One Clear erythema Clear edema 2 2 Medium strong erythema Moderate edema (about 1 mm) 3 3 Deep red erythema Strong edema (more than 1 mm) 4 4 Stability Classification Primary irritant 00.1 ~ 0.50.6 ~ 1.51.6 ~ 3.03.1 ~ 5.05.1 ~ 6.56.6 ~ 8.0 No irritation In practical use No irritation Mild irritation Mild irritation Normal irritation Partial irritation

(6) Test result

Pharmacopoeia saponin skin accumulation test result Observation time Healthy skin Damaged skin 30 minutes later Erythema edema Etc Erythema edema Etc 24 hours later 0 0 - 0 0 - 48 hours later 0 0 - 0 0 - After 72 hours 0 0 - 0 0 - sub Total 0 0 - 0 0 - Average 0 0 - 0 0 - Sum of average 0 0 Cumulative Skin Irritation Index 0 (no stimulation) 0 (no stimulation)

Pharmacopoeia saponin cream skin accumulation test result Observation time Healthy skin Damaged skin Erythema edema Etc Erythema edema Etc 30 minutes later 0 0 - 0 One - 24 hours later 0 0 - 0 0 - 48 hours later 0 0 - 0 0 - After 72 hours 0 0 - 0 0 - sub Total 0 0 - 0 One - Average 0 0 - 0 0.25 - Sum of average 0 0.25 Cumulative Skin Irritation Index 0 (no stimulation) 0.25 (no stimulus in practical use)

 Control cream cumulative test results Observation time Healthy skin Damaged skin Erythema edema Etc Erythema edema Etc 30 minutes later 0 0 - One 2 - 24 hours later 0 0 - 0 One - 48 hours later 0 0 - 0 0 - After 72 hours 0 0 - 0 0 - sub Total 0 0 - One 3 - Average 0 0 - 0.25 0.75 - Sum of average 0 One Cumulative Skin Irritation Index 0 (no stimulation) 1 (mineral stimulus)

As can be seen in Tables 6 to 8, the control cream showed some irritation to skin accumulation, but the weak soy saponin and weak soy saponin cream according to the present invention showed little irritation even in cumulative use.

Example 9 Phototoxicity Test

In Experimental Example 8 (3) in the test method in the morning and evening a day, the test substance (weak soy saponin, weak soy saponin cream, control cream) was applied twice in 0.5g and irradiated with UV lamp 16 It was carried out for a day. The control compartment was left in the air (not irradiated with UV lamp).

Pharmacopoeia saponin phototoxicity test result Observation time Healthy skin Damaged skin Erythema edema Etc Erythema edema Etc 30 minutes later 0 0 - One 0 - 24 hours later 0 0 - 0 0 - 48 hours later 0 0 - 0 0 - After 72 hours 0 0 - 0 0 - sub Total 0 0 - One 0 - Average 0 0 - 0.25 0 - Sum of average 0 0.25 Skin irritation index 0 (no stimulation) 0.25 (no stimulus in practical use)

Pharmacopoeia saponin cream phototoxicity test result Observation time Healthy skin Damaged skin Erythema edema Etc Erythema edema Etc 30 minutes later One 0 - One 0 - 24 hours later 0 0 - One 0 - 48 hours later 0 0 - 0 0 - After 72 hours 0 0 - 0 0 - sub Total One 0 - 2 0 - Average 0.25 0 - 0.5 0 - Sum of average 0.25 0.5 Skin irritation index 0.25 (no stimulus in practical use) 0.5 (no stimulus in practical use)

 Control Cream Phototoxicity Test Results Observation time Healthy skin Damaged skin Erythema edema Etc Erythema edema Etc 30 minutes later 0 0 - One 2 - 24 hours later 0 0 - One One - 48 hours later 0 0 - 0 0 - After 72 hours 0 0 - 0 0 - sub Total 0 0 - 2 3 - Average 0 0 - 0.5 0.75 - Sum of average 0 1.25 Skin Primary Irritation Index 0 (no stimulation) 1.25 (minor stimulus)

As can be seen in Tables 9 to 11, the control cream showed some irritation to light exposure, but the weak soy saponin and weak soy saponin cream according to the present invention showed little irritation.

Saponin extracted from medicinal soybean is excellent in efficacy and safety in wrinkle improvement and whitening ingredients. Therefore, the soybean-derived saponin can be safely used as a basic raw material of cosmetics as well as functional cosmetic raw materials for wrinkle improvement and whitening. In addition, the soybean-derived saponins can replace expensive ginseng saponins and are economically useful.

1 is a graph showing the effect of free radical scavenging of the saponin derived from soybean according to the present invention.

Figure 2 is a graph showing the antioxidant effect of the soybean-derived saponin of the present invention.

Figure 3 is a graph showing the sunscreen effect of the medicinal soybean-derived saponin according to the present invention.

Figure 4 is a photograph showing the anti-wrinkle effect of the soybean-derived saponin according to the present invention.

Figure 5 is a photograph showing the whitening effect of medicinal soybean-derived saponin according to the present invention.

Claims (3)

Functional cosmetic composition containing saponin extracted from medicinal beans as an active ingredient. The cosmetic composition according to claim 1, wherein the functionality has a whitening effect through anti-melanin activity. The cosmetic composition according to claim 1, wherein the functionality has the effect of anti-wrinkle and / or improvement through antioxidant activity, anti-oxidation activity or sun protection activity.