KR100533520B1 - A skin-care agent containing Veronica Peregrina L. extract - Google Patents

Abstract

The present invention relates to an external composition for skin containing Veronica peregrina L. extract, and more specifically, 0.001 to 30.0 wt% of Veronica peregrina L. extract, which is an anti-aging, irritant and skin whitening agent. The present invention relates to an external skin composition having excellent effects.

Description

A skin-care agent containing Veronica Peregrina L. extract

The present invention relates to an external composition for skin containing jujucho extract, more specifically, 0.001-30.0% by weight of jujucho extract, antioxidant effect, effective in inhibiting the activity and expression control of matrix metalloproteinase, anti-inflammatory effect The present invention relates to an external composition for skin having excellent anti-aging, stimulating effect and whitening effect, which are effective in inhibiting tinosinase activity, inhibiting melanin synthesis, and the like.

Skin aging can be divided into intrinsic chronological and phtoaging [Gilchrest BA: J. Am. Acad. Dermatol., 21, 610-613 (1989). Endogenous aging is a naturally occurring aging phenomenon caused by deterioration of physiological functions of living bodies with increasing age [Braverman IM et al .: J. Invest. Dermatol., 78, 434-443 (1982). Photoaging means that the skin is repeatedly exposed to light to change the appearance or function of the skin [Ridder GM et al .: J. Am. Acad. Dermatol., 25, 751-760 (1991). In addition, skin aging may be caused by the activation of free radicals with ultraviolet rays, stress, disease conditions, environmental factors, wounds, and age, and when this condition is exacerbated, it destroys the antioxidant defense network existing in vivo, cells and Damage to tissues promotes adult disease and aging. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging.

Matrix metalloprotease (MMP) is a family of enzymes that plays a major role in the physiological and pathological destruction of connective tissues, especially collagen. Various types of collagen and collagenase (MMP type) are known in the art and details can be found in US patent application Ser. No. 08 / 588,771. Inhibitors of MMPs (ie, direct inhibitors of proteinases) and inhibitors of molecular pathways (ie, inhibitors of AP-1) that affect MMP expression are known in other fields and are also described in US Pat. No. 588,771.

Retinoids have been used as a treatment to improve the appearance of skin damaged by sunlight. U.S. Patent 4,877,805 discloses a method of treating skin that is aged by light. The patent suggests a weakness in applying retinoids to treat photodamage until the effects of aging begin to emerge. Various studies have been conducted to improve the appearance of existing photodamaged skin using all trans retinoic acid [Weinstein G.D. et al., Arch. Dermatol., 127,659-665 (1991); Weiss J.S. Et al., J. Amer. Med. Assn., 259 (4), 527-532 (1988).

Retinoic acid inhibits lipid per-oxidation by UV-A and ornithine decarboxylase reponse by UV-B and improves skin photodamage [Francz PI et al., Biol. Chem., 379 (10), 1263-1269 (1998). However, skin irritation is so strong that it is currently used only clinically. FDAs in the U.S. and Korea prohibit their use as cosmetic ingredients.

Retinol (Vitamin A) improves the wrinkles of the skin compared to retinoic acid, but the safety of the material is the most widely used anti-wrinkle agent. However, retinol also causes problems such as skin irritation when used in high concentrations, especially when exposed to ultraviolet light is known to cause strong skin problems. Furthermore, retinol has a disadvantage in that its stability is very low and is easily oxidized by oxygen, light and heat in the air (Korean Patent Publication No. 10-0271315).

Next is skin change by pigmentation. The skin normally produces melanin to protect the skin from the sun's ultraviolet rays. Melanin is produced from melanocytes, a kind of skin cells, and has a natural screening function that is distributed on the surface of the skin and blocks ultraviolet rays harmful to the human body. There is an enzyme called tyrosinase that is synthesized in melanocytes. Tyrosinase generates dopaquinone via dopa (DOPA) based on an amino acid called tyrosine, and melanin, which is a black pigment, is produced by several stages of oxidative condensation reaction from dopaquinone. Ascorbic acid (JP-A 4-9320), hydroquinone (JP-A-6-192062), kojic acid (JP-A-56-7710), arbutin (JP-A 4-9315) and some Although extracts have tyrosinase inhibitory activity and are used as whitening cosmetics, their use is limited due to poor stability in the prescription system, discoloration due to poor coloration, off-flavor, efficacy at the biological level, unclear effect and safety issues. It's happening. And the inhibitory effect of tyrosinase has been demonstrated, but the effect is low in experiments similar to the actual biologic level.

Recently, in order to reduce skin irritation caused by various chemicals, a lot of attention has been focused on functional cosmetics having functions such as antioxidants, wrinkle reduction, and whitening obtained from natural extracts. Natural ingredients have less side effects on the skin, and as the consumer's response to cosmetics using natural ingredients has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing. For example, US Pat. No. 5,972,341 describes the antiwrinkle effect of a plant of the genus Commiphora, in particular Commiphora mukul extract. Japanese Patent Application Laid-open No. Hei 9-672662 describes seaweed extracts having a hyaluroronidase inhibitory effect. Japanese Patent Application Laid-open No. Hei 10-85905 describes the skin texture improvement effect of Fucoidan extracted from Wakame. Japanese Patent Application Laid-Open No. 2-245087 describes the antioxidant effect of Sargassum genus extract; Japanese Patent Application Laid-Open No. 3-294384 describes an antioxidant composition extracted from the genus Hijikia. Japanese Patent Laid-Open No. 7-10769 describes a extract of Phaeophyceae river having an astringent effect.

According to China's Chinese herbaceous plant, judocho is called silk grass, naegumcho and spotted grass, and it is now, ground, super blood gallon and blood ) It has various names such as Ogongcho (蜈蚣 草), and is a yearly small arboreous tree with stems and branches that are delicate and pale crimson hairs.

An object of the present invention is to provide a skin external preparation having excellent functionality by selecting a fresh seed among various natural products and using it as a raw material of the skin external preparation.

In other words, it contains the extract of judochocho as an active ingredient, and it has excellent anti-aging effects such as antioxidant effect, collagenase expression inhibition effect, skin wrinkle relief effect, excellent whitening effect such as tyrosinase inhibitory effect and melanogenesis inhibitory effect. It is to provide an external skin composition for showing an excellent effect in the skin irritation-reducing effect and the like.

In order to achieve the above object, the present inventors have studied the possibility of applying as a skin external preparation among various natural products, the extract of juichocho extract antioxidant effect, collagenase expression inhibitory effect, skin wrinkle relief effect, tyrosinase activity inhibitory effect, As a result of measuring melanin synthesis inhibitory effect and stimulating effect, it was confirmed that the anti-aging, whitening and stimulating effect is excellent, and prepared the external preparation composition containing the same.

The present invention relates to an external composition for skin containing Veronica peregrina L. extract.

In addition, the present invention is characterized in that the extract of Verona peregrina L. is contained in 0.001 ~ 30.0% by weight based on the total weight of the total composition. If the content of the extract is less than 0.01% by weight, the efficacy is weak, and if the content exceeds 30.0% by weight it is not economical because there is no apparent increase in efficacy as the content increases.

In addition, the present invention using the at least one selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane as the solvent, the cold extract, heating at room temperature A liquid obtained by filtration, and further characterized in that it is obtained by concentration under reduced pressure or lyophilization.

In addition, the present invention is characterized in that the topical skin composition is a cosmetic composition or a pharmaceutical composition.

In addition, the present invention is characterized in that the cosmetic composition is formulated in a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type or water-in-oil (W / O) type.

Derivative extract can be formulated into pharmaceutical compositions, such as capsules, liquids, ointments, patch or sustained-release agent using a pharmaceutically acceptable carrier, pharmacologically acceptable bases, carriers, excipients, binders ( Examples: starch, tragacanth gum, gelatin, molasses, polyvinyl alcohol, polyvinylether, polyvinyl pyrrolidone, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose and carboxymethyl cellulose), grinding agents (e.g., agar, Starch, gelatin powder, sodium carboxymethyl cellulose, calcium carboxymethyl cellulose, crystalline cellulose, calcium carbonate, sodium bicarbonate and sodium alginate), lubricants (e.g. magnesium stearate, talc, hydrogenated vegetable oils), colorants and the like. The carriers and excipients include lactose, glucose, sucrose, mannitol, potato starch, corn starch, calcium carbonate, calcium phosphate and cellulose.

In addition to the above, additives such as adjuvants such as stabilizers, dissolution aids, transdermal absorption accelerators, and preservatives may be further added.

The pharmaceutical composition prepared as described above may be applied to the skin once or several times a day depending on the symptoms and may adjust the amount applied according to the improvement of symptoms.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are merely illustrative of the present invention and the scope of the present invention is not limited to these examples.

Example 1: Preparation of juniper extract

The dried and dried fresh lead herb was refluxed three times for 5 hours with an aqueous 95% (V / V) ethanol solution, and the mixture was cooled and then filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50 ° C. or lower, and the dried extract was prepared using one or more solvents selected from purified water, ethanol, butylene glycol, and propylene glycol to contain 0.001 to 30.0 wt% of the reduced pressure concentrate and lyophilisate. An extract was prepared.

Experimental Example 1 Experiment of Measuring Antioxidant Effect Using NBT Method

In order to confirm the antioxidant effect of the extract of judochocho obtained in Example 1, BHT, which is well known as an antioxidant under laboratory conditions, was compared and the antioxidant activity was measured using NBT (Nitro Blue Tetrazolium) method.

In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase is measured by the NBT method, and the effect of the test substance removing the active oxygen, that is, the active oxygen scavenging effect, is evaluated. Free radicals are produced by xanthine and xanthine oxidase. This active oxygen reacts with Nitro Blue Tetrazolium (NBT) to measure the active oxygen scavenging rate by measuring the blue color produced by this at a wavelength of 560 nm.

As a measuring method

0.05M Na ₂ CO ₃ ------------------------------------- 2.4ml

 2.3mM xanthine solution ---------------------------------- 0.1ml

 3.3mM EDTA Solution ------------------------------------ 0.1ml

 4.BSA solution ---------------------------------------- 0.1ml

 5.0.72 mM NBT solution ---------------------------------- 0.1ml

 6.Xanthine Oxidase Solution ----------------------------- 0.1ml

7.6 mM CuCl ₂ solution ----------------------------------- 0.1ml

① Add 1,2,3,4,5 to Bayer's bottle and add 0.1ml of sample solution to it and leave at 25 ℃ for 10 minutes.

② Add 6 and stir quickly, and start incubation for 20 minutes at 25 ℃.

③ After that, add 7 to stop the reaction and measure the absorbance St at 560nm.

④ For the blank test, measure the absorbance Bt by using distilled water instead of the sample solution as above.

⑤ Also, the blank of the sample solution is operated in the same manner using distilled water instead of 6 to measure the absorbance Bo.

The effect was calculated by Equation 1, and the results are shown in Table 1.

% Inhibition = [1- (St-So) / (Bt-Bo)] X 100

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction without enzyme addition of sample solution

Bo: absorbance at 560 nm before reaction without enzyme in blank test solution

As shown in Table 1, jujucho extract showed an excellent antioxidant effect, judocho extract was confirmed to have a superior antioxidant power than BHT.

Sample Name Antioxidative effect(%) Udoncho extract 0.1% by weight 97% 0.01% by weight of judocho extract 77% 0.001% by weight of fresh seaweed extract 37% BHT 0.1 wt% 88%

Experimental Example 2: Free radical scavenging activity measurement experiment

In order to determine the antioxidant effect of the extract of judochocho obtained in Example 1, an antioxidant such as BHT was compared with a sample and the antioxidant activity was measured using the DPPH method.

DPPH method measures the antioxidant activity by reducing power using a free group called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical). The free radical scavenging rate is measured at a wavelength of 560 nm by comparing the degree to which the DPPH is reduced by the test substance to reduce the absorbance.

For the measurement of DPPH free radical scavenging activity, the extracts of the vinegar were prepared in 0.1%, 0.01%, 0.001% concentration. Extracts of the above concentrations were placed in 96-well plates, respectively, and DPPH prepared in 100 uM ethanol solution was added to obtain a total volume of 200 μl. After leaving it at 37 ° C. for 30 minutes, absorbance was measured at 560 nm. Free radical scavenging activity (%) was calculated by the following equation.

Free radical scavenging activity (%) = 100- (B / A) X100

A: absorbance of control wells not treated with the extract of the present invention

B: Absorbance of Experimental Wells Treated with Extracts of the Invention

As can be seen in Table 2, the extract of judochocho was found to have an excellent antioxidant effect than BHT which is widely used as an antioxidant.

Sample Name Antioxidative effect(%) Udoncho extract 0.1% by weight 93% 0.01% by weight of judocho extract 63% 0.001% by weight of fresh seaweed extract 41% BHT 0.1 wt% 85%

Experimental Example 3: In vitro MMP-1 Inhibition Assay

Substrate metalloproteinase (MMP-1) inhibitory activity was measured in vitro in a screening type biochemical model based on the use of collagen and gelatin conjugated to purified collagenase and its substrate, fluorescein. (EnzChek ™ Gelatinase / Collagenase Kit, Molecule Prove). Collagenase purified from Clostridium historiescom was supplied in the EnzChek ™ gelatinase / collagenase kit. The reaction buffer consisting of DQ-collagen purified from porcine skin and conjugated to fluorescein with 0.05M Tris-HCl, 0.15M NaCl, 5mM CaCl ₂ and 0.2mM sodium azide (pH 7.6) was identified as EnzChek® gelatinase. Collagenase kit (Molcula Probes) was used. The fresh seaweed extract was dissolved in the reaction buffer. It was tested at 0.01%, 0.5%, 0.1% by weight. Dilutions of the test extracts were incubated with 25 μg / ml of DQ-collagen and 0.1 U / ml of collagenase for 15 minutes, 45 minutes, and 120 minutes at room temperature.

In each experimental condition, the control corresponding to the collagenase and DQ-collagen mixture was also incubated in the same manner. Also in each experimental condition a sample called Blank, hereinafter 'enzyme free blank', was incubated in the presence of DQ-collagen and in the absence of collagenase. Each experiment was performed three times.

After 15, 45 and 120 minutes, the signal corresponding to the degradation of DQ-collagen was measured with a fluorometer (excitation: 485 nm, emission 505 nm). The fluorescence value of each sample was measured based on the 'enzyme free blank' fluorescence value. In conclusion, the herbaceous extract tested at 0.01 to 0.1% by weight under selected experimental conditions retained dose dependent anticollagenase / gelatinase activity.

The results are shown in Table 3. When 0.1% by weight of judocho extract, the collagen degrading activity of Clostridium collagenase was inhibited by 78%, which was similar to the inhibitory effect of green tea extract.

Sample Name % Inhibition Udoncho extract 0.1% by weight 78 Udoncho extract 0.05% by weight 59 0.01% by weight of judocho extract 32 0.2% by weight of green tea extract 79

Experimental Example 4: Evaluation of the inhibition of MMP-1 expression by the extract of judo vinegar after ultraviolet irradiation

In this experimental example, ELISA was performed to measure the concentration of MMP-1 after UV irradiation and sample addition of the fresh seaweed extract obtained in Example 1.

Human dermal fibroblasts were irradiated with UVA at an energy of 6.3 J / cm 2 using a UV chamber. UV irradiation amount and incubation time were established through preliminary experiments to maximize the expression of MMP in fibroblasts. The negative control was wrapped in tinfoil to keep the same time in the environment of UVA. UVA emission was measured using a UV radiometer. The cells during the UVA irradiation were intact in the previously dispensed medium and irradiated with UVA, exchanged with the medium containing the sample, and then cultured for 24 hours, and the medium was recovered and coated in 96 wells. The primary antibody (MMP-1 (Ab-5) monoclonal antibody) was treated and reacted at 37 ° C. for 60 minutes. After reacting the secondary antibody anti-mouse IgG (whole mouse, alkaline phosphatase conjugated) again for about 60 minutes, the alkaline phosphatase substrate solution (1 mg / ml ρ-nitrophenyl phosphate in diethanolamine buffer solution) was reacted at room temperature for 30 minutes and then Absorbance was measured at 405 nm with a plate reader. As a control, one without addition of a sample was used.

Compared with the control group that did not process the MMP-1 expression induced by UV irradiation, the extract of judochocho showed more than 68% inhibition rate, which was significantly superior to the inhibition rate of the retinol used as a control group (Table 4). ).

Test group MMP-1 expression inhibition rate (%) Control - Udoncho extract 0.05% by weight 68 0.01% by weight of judocho extract 36 Retinol 47

Experimental Example 5: Inhibition of Tyrosinase Activity

Tyrosinase was isolated and purified from mushrooms and purchased from Sigma. Substrate L-tyrosine (Sigma) was dissolved in 0.05M sodium phosphate buffer (pH 6.8) to make a 0.1mg / ㎖ solution was used. Judocho extract was used after dissolving in an appropriate concentration in 0.05M sodium phosphate buffer (pH 6.8). 0.5 ml of L-tyrosine solution was placed in a test tube, and 0.5 ml of fresh vinegar extract sample solution was added thereto and left for 10 minutes in a 37 ° C thermostat. Thereafter, 0.5 ml of 200 unit / ml tyrosinase was added to each sample solution, and the mixture was reacted at 37 ° C for 10 minutes. The test tube containing the reaction solution was placed on ice, quenched to stop the reaction, and the spectrophotometer measured absorbance at a wavelength of 475 nm. As a control, 0.5 ml of buffer solution was used instead of the sample.

The tyrosinase activity inhibition rate of each sample was calculated according to Equation 3 and the results are shown in Table 5.

Enzyme Inhibition Rate (%) = 100- (Comparative Absorption / Control Absorption) X100

Sample Name Tyrosinase inhibition rate (%) Control - Udoncho extract 0.05% by weight 61% 0.01% by weight of judocho extract 29% Arbutin 0.2 wt% 63%

Experimental Example 6: Melanin synthesis inhibition experiment using B16F1 melanocytes

The melanin synthesis inhibitory effect on B16F1 melanocytes was measured in order to confirm the whitening effect of the extract of judochocho obtained in Example 1.

The melanin synthesis inhibitory effect of B16F1 melanocytes was measured as follows. B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 x 10 ⁶ cells per well, and the cells were attached and incubated for 72 hours by treating the sample at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, the number of cells was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Rotan: Cancer Res. , 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. Melanin was extracted by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and then measured the absorbance of melanin at 405 nm with a microplate reader. Percent inhibition of production was measured. Melanin production inhibition rate (%) of B16F1 melanocytes was calculated by the equation (4).

% Inhibition = [(A-B) / A] X 100

A: Melanin amount in wells without sample

B: Melanin amount in the wells to which the sample was added

As a result of testing the melanin synthesis inhibitory effect of B16F1 melanin cells, the extract of jujucho showed a melanin production inhibitory effect of 0.05% to 25%, showing a similar production inhibitory effect to arbutin (Table 6).

Sample Name Melanin Synthesis Inhibitory Effect (%) Control - Udoncho extract 0.05% by weight 25% 0.01% by weight of judocho extract 16% Arbutin 0.2 wt% 27%

Experimental Example 7: Inhibitory Effect of Prostaglandin Biosynthesis by UV Irradiation

In this experimental example, in order to evaluate the inhibitory effect of prostaglandin biosynthesis of judochori extract obtained in Example 1, 5 × 10 ⁴ keratinocytes isolated from epidermal tissue were put on a 24-well test plate and attached for 24 hours. I was. After replacing with FBS-free medium and incubating for 18 hours, prostaglandin biosynthetic enzymes (prostaglandin E ₂ synthetase) present in keratinocytes were treated with aspirin to achieve a final concentration of 50 uM on the keratinocytes. , Or cyclooxygenase (hereinafter referred to as COX) was removed. Two hours after aspirin treatment, each well containing keratinocytes was washed twice with PBS and 100 μl of PBS was added to each well. The keratinocytes were irradiated with UV 30 mJ / cm 2 using a UVB lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then PBS was removed and each cell was prepared with keratinocyte growth media (Clonetics, Biowhittacker, MD, USA) 250 μl was added. After treatment with the extract of the vinegar to be evaluated here was incubated for 16 hours. An appropriate amount of the culture supernatant was taken to quantify PGE ₂ (prostaglandin E ₂ ) biosynthesized for 16 hours to determine the prostaglandin inhibitory effect of the extract of Judo. The amount of PGE ₂ was quantified using an enzyme immunoassay, and the results showed that the extract of edodes effectively inhibited the production of prostaglandins by UV light. Particularly, the produced PGE ₂ is known to be mainly produced by the COX-2 enzyme, and thus, it can be seen from the above experiment that the extract of judocho inhibits the induction or activity of the COX-2 enzyme (Table 7).

Sample Name PGE ₂ Inhibition Rate (%) (-) UV B - (+) UV B - (+) UV B + fresh vinegar extract 0.05% by weight 67% (+) UV B + fresh seaweed extract 0.01% by weight 25%

Examples 2 to 4 and Comparative Example 1

A cosmetic containing the extract of judochocho obtained in Example 1 was prepared and evaluated for the effect of improving skin wrinkles and skin beauty by performing a comparative experiment with Comparative Example 1.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 8. First, the phase b) recorded in Table 8 is heated and stored at 70 ° C. To this, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer, and then cooled slowly to prepare a cream (Examples 2 to 4, Comparative Example 1). 20 experimenters (20-35 year old female) applied the cream prepared in Examples 2 to 4 on the right side of the face and the cream prepared in Comparative Example 1 twice on the left side of the face for 3 consecutive months. It was.

After completion of the experiment, the skin wrinkle relief effect was collected before and after using the product for 2 months by using a silicone resin copy (replica) around the face of the eyelids. Compare the condition of fine lines around the eyes.

Raw material Example 2 Example 3 Example 4 Comparative Example 1 end Stearyl alcohol 8 8 8 8 Stearic acid 2 2 2 2 Stearic cholesterol 2 2 2 2 Squalane 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 3 Glyceryl monostearic acid ester 2 2 2 2 I Fresh seaweed extract 10 One 0.1 - Propylene glycol 5 5 5 5 Purified water Quantity Quantity Quantity Quantity

Note) Unit: Weight%

Table 9 compares the average eye wrinkle depth and wrinkle reduction rate of the experimenter using the cream prepared in Example 4 with the experimenter using the cream of Comparative Example 1. As shown in Table 9, it can be seen that the skin wrinkle improvement effect is excellent in the skin around the facial eye of the experimenter applying the cream containing the extract of judocho.

Example 4 Comparative Example 1 Before use 2 months later Before use 2 months later Average fine wrinkle depth (AU) 0.194 0.167 0.191 0.187 Wrinkle Reduction (%) 13.92% 2.09%

n = 20, p <0.01

Examples 5 to 7 and Comparative Example 2

This Example prepared the cosmetic containing the extract of judochocho obtained in Example 1 and evaluated the skin whitening effect in a comparative experiment with Comparative Example 2 in humans.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 10. First, the phase b) recorded in Table 10 is heated and stored at 70 ° C. To this, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer, and then cooled slowly to prepare a cream (Examples 5 to 7, Comparative Example 2). 20 experimenters (women aged 20-35 years) were treated with creams prepared in Examples 5 to 7 on the right side of the face, and creams prepared in Comparative Example 2 on the left side of the face for 2 consecutive days. Applied. After completion of the test, the skins of the application areas on both sides of the face were compared by using an image analyzer and a color difference meter. Table 11 compares the facial skin color of the experimenter using the cream prepared in Example 7 with the facial skin color of the experimenter using the cream made in Comparative Example 2.

As shown in Table 11, it can be seen that the whitening effect is excellent in the facial skin of the experimenter applying the cream containing the extract of judocho.

Raw material Example 5 Example 6 Example 7 Comparative Example 2 end Stearyl alcohol 8 8 8 8 Stearic acid 2 2 2 2 Stearic cholesterol 2 2 2 2 Squalane 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 3 Glyceryl Monostearic Acid Ester 2 2 2 2 I Fresh seaweed extract 10 One 0.1 - Propylene glycol 5 5 5 5 Purified water Quantity Quantity Quantity Quantity

Note) Unit: Weight%

Experiment No. Example 7 Comparative Example 2 Right skin color Left skin color One 2 3 2 2.5 3 3 1.5 2 4 2 2.5 5 2.5 3 6 2 2.5 7 3 3.5 8 2 3 9 2.5 3 10 2.5 3.5 11 One 2 12 2.5 3 13 One 2 14 2 3.5 15 2 2 16 2.5 3 17 3 3 18 2 3.5 19 2.5 3 20 2 3

Other examples are shown below. That is, the lotion, milky lotion and essence containing the extract of judochocho obtained in Example 1 were prepared in Examples 8 to 10. The lotion, milky lotion and essence containing the extract of jujucho showed excellent effects on skin improvement such as antioxidant effect and skin wrinkle improvement.

Example 8: Preparation of a Lotion

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are dissolved. . 0.05 g of judo herb extract obtained in Example 1 and 5 g of glycerine were dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a lotion having a skin improvement effect.

Example 9: Preparation of Latex

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. , 0.5 g of the fresh lead extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were heated to 75 ° C. to dissolve it. Both mixtures were emulsified and cooled to obtain an emulsion having an oil-in-water (O / W) type skin improvement effect.

Example 10 Preparation of Cosmetic Liquid

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, 1 g of judocho extract obtained in Example 1 and a suitable amount of pigment was mixed to obtain a cosmetic liquid having an effect of improving skin.

As described above, the present invention provides an excellent anti-aging effect such as antioxidant effect, MMP inhibitory effect, MMP expression control effect by UV irradiation, fine wrinkle improvement effect and skin irritation caused by UV irradiation, blemishes, freckles and It is possible to provide a fresh skin extract extract external skin composition for whitening effects such as melanin production inhibitory, tyrosinase activity inhibitory, which is a substance causing skin pigmentation.

In addition, the present invention can be provided as a cosmetic composition such as a lotion, cream, emulsion, pack, powder, etc. as an active ingredient extract of judocho extract based on the above effects.

Claims (5)

Skin external composition containing the extract of Veronica Peregrina L .. According to claim 1, wherein the judocho extract is an external skin composition containing judocho extract, characterized in that contained in 0.001 ~ 30.0% by weight based on the total weight of the total composition. According to claim 1 or claim 2, wherein the fresh extract is a solvent using one or more selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane as a solvent A skin external preparation composition containing fresh grass extract, which is obtained by cooling at room temperature, heating and filtration, and further obtaining a solvent under reduced pressure or lyophilization. The external skin composition according to claim 1 or 2, wherein the external skin composition is a cosmetic composition or a pharmaceutical composition. According to claim 4, wherein the cosmetic composition is a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type or water-in-oil (W / O) type judocho extract characterized in that Skin external preparation composition containing.