KR101451955B1 - 액적 작동기 상에서의 비드 조작법 - Google Patents

액적 작동기 상에서의 비드 조작법 Download PDF

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Publication number
KR101451955B1
KR101451955B1 KR1020107005688A KR20107005688A KR101451955B1 KR 101451955 B1 KR101451955 B1 KR 101451955B1 KR 1020107005688 A KR1020107005688 A KR 1020107005688A KR 20107005688 A KR20107005688 A KR 20107005688A KR 101451955 B1 KR101451955 B1 KR 101451955B1
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South Korea
Prior art keywords
droplet
delete delete
barrier
beads
droplet actuator
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Expired - Fee Related
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KR1020107005688A
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English (en)
Korean (ko)
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KR20100133943A (ko
Inventor
프라사나 쓰워
드웨인 앨런
필립 백
Original Assignee
어드밴스드 리퀴드 로직, 아이엔씨.
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424—Dielectrophoretic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Fluid Mechanics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
KR1020107005688A 2007-08-24 2008-08-25 액적 작동기 상에서의 비드 조작법 Expired - Fee Related KR101451955B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US95771707P 2007-08-24 2007-08-24
US60/957,717 2007-08-24
US98076707P 2007-10-17 2007-10-17
US60/980,767 2007-10-17
PCT/US2008/074151 WO2009029561A2 (en) 2007-08-24 2008-08-25 Bead manipulations on a droplet actuator

Publications (2)

Publication Number Publication Date
KR20100133943A KR20100133943A (ko) 2010-12-22
KR101451955B1 true KR101451955B1 (ko) 2014-10-21

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KR1020107005688A Expired - Fee Related KR101451955B1 (ko) 2007-08-24 2008-08-25 액적 작동기 상에서의 비드 조작법

Country Status (10)

Country Link
US (2) US8591830B2 (https=)
EP (1) EP2188059B1 (https=)
JP (2) JP5302966B2 (https=)
KR (1) KR101451955B1 (https=)
CN (1) CN101808751B (https=)
AU (1) AU2008293652B2 (https=)
BR (1) BRPI0815698A2 (https=)
CA (1) CA2696604A1 (https=)
MX (1) MX2010002079A (https=)
WO (1) WO2009029561A2 (https=)

Families Citing this family (102)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1859330B1 (en) 2005-01-28 2012-07-04 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
WO2009140671A2 (en) 2008-05-16 2009-11-19 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
EP2573562A3 (en) 2007-02-09 2013-10-30 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
EP2109774B1 (en) 2007-02-15 2018-07-04 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
WO2009032863A2 (en) 2007-09-04 2009-03-12 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US9631244B2 (en) 2007-10-17 2017-04-25 Advanced Liquid Logic, Inc. Reagent storage on a droplet actuator
CA2709928A1 (en) 2007-12-23 2009-07-09 Advanced Liquid Logic, Inc. Droplet actuator configurations and methods of conducting droplet operations
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US8877512B2 (en) * 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
GB0913258D0 (en) 2009-07-29 2009-09-02 Dynex Technologies Inc Reagent dispenser
US9523701B2 (en) 2009-07-29 2016-12-20 Dynex Technologies, Inc. Sample plate systems and methods
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
WO2011057197A2 (en) 2009-11-06 2011-05-12 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel electrophoresis and molecular analysis
EP2516669B1 (en) 2009-12-21 2016-10-12 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
EP2553473A4 (en) 2010-03-30 2016-08-10 Advanced Liquid Logic Inc DROPLET OPERATION PLATFORM
US10232374B2 (en) 2010-05-05 2019-03-19 Miroculus Inc. Method of processing dried samples using digital microfluidic device
EP2588322B1 (en) 2010-06-30 2015-06-17 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
WO2012154745A2 (en) 2011-05-09 2012-11-15 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
US9140635B2 (en) 2011-05-10 2015-09-22 Advanced Liquid Logic, Inc. Assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
KR20130009504A (ko) 2011-07-15 2013-01-23 삼성전자주식회사 개구 조절 방법 및 개구 조절 소자
WO2013016413A2 (en) 2011-07-25 2013-01-31 Advanced Liquid Logic Inc Droplet actuator apparatus and system
WO2013070627A2 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
WO2013078216A1 (en) 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
DE102012002272A1 (de) 2012-02-04 2013-08-08 Clariant International Ltd. Pestizid-Zusammensetzungen
ES2599504T3 (es) 2012-05-30 2017-02-02 Clariant International Ltd Utilización de N-metil-N-acil-glucaminas como agentes solubilizantes
IN2014DN09937A (https=) 2012-05-30 2015-08-14 Clariant Int Ltd
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
EP2867645B1 (en) 2012-06-27 2019-06-05 Advanced Liquid Logic, Inc. Techniques and droplet actuator designs for reducing bubble formation
US10316872B2 (en) 2012-08-27 2019-06-11 The Board Of Trustees Of The Leland Stanford Junior University Two-dimensional magnetic trap arrays for droplet control
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
JP1628115S (https=) 2012-10-24 2019-04-01
WO2014078100A1 (en) * 2012-11-02 2014-05-22 Advanced Liquid Logic, Inc. Mechanisms for and methods of loading a droplet actuator with filler fluid
DE102012021647A1 (de) 2012-11-03 2014-05-08 Clariant International Ltd. Wässrige Adjuvant-Zusammensetzungen
US9366647B2 (en) * 2013-03-14 2016-06-14 Taiwan Semiconductor Manufacturing Company, Ltd. Optical detection for bio-entities
US11029310B2 (en) 2013-03-14 2021-06-08 Wisconsin Alumni Research Foundation Device and method for extracting a targeted fraction from a sample
CN107866286A (zh) 2013-03-15 2018-04-03 金马克诊断股份有限公司 用于操纵可变形流体容器的系统、方法和设备
KR101997258B1 (ko) * 2013-06-28 2019-10-01 엘지이노텍 주식회사 발광 소자 패키지
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
KR101480651B1 (ko) * 2013-12-09 2015-01-09 현대자동차주식회사 오브젝트 처리 방법 및 이를 지원하는 차량
DE202014008415U1 (de) 2014-02-19 2014-11-25 Clariant International Ltd. Wässrige Adjuvant-Zusammensetzung zur Wirkungssteigerung von Elektrolyt-Wirkstoffen
DE102014005771A1 (de) 2014-04-23 2015-10-29 Clariant International Ltd. Verwendung von wässrigen driftreduzierenden Zusammensetzungen
US20150306598A1 (en) * 2014-04-25 2015-10-29 Berkeley Lights, Inc. DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
CA2945177C (en) * 2014-04-25 2021-12-21 Berkeley Lights, Inc. Providing dep manipulation devices and controllable electrowetting devices in the same microfluidic apparatus
US11192107B2 (en) * 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
DE102014012022A1 (de) 2014-08-13 2016-02-18 Clariant International Ltd. Organische Ammoniumsalze von anionischen Pestiziden
EP3218108B1 (en) 2014-11-11 2020-09-02 Genmark Diagnostics Inc. Fluid sample processing cartridge and use thereof
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
DE102014018274A1 (de) 2014-12-12 2015-07-30 Clariant International Ltd. Zuckertenside und deren Verwendung in agrochemischen Zusammensetzungen
ES2890657T3 (es) 2014-12-19 2022-01-21 Clariant Int Ltd Composiciones adyuvantes acuosas que contienen electrolitos, composiciones que contienen principios activos y su uso
JP6622811B2 (ja) 2014-12-31 2019-12-18 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ デジタルマイクロ流体希釈装置、システムおよび関連方法
US10723988B2 (en) 2015-04-22 2020-07-28 Berkeley Lights, Inc. Microfluidic cell culture
CN108026494A (zh) 2015-06-05 2018-05-11 米罗库鲁斯公司 限制蒸发和表面结垢的空气基质数字微流控装置和方法
US10695762B2 (en) 2015-06-05 2020-06-30 Miroculus Inc. Evaporation management in digital microfluidic devices
NL2015287B1 (en) * 2015-08-10 2017-02-28 Micronit Microfluidics Bv Channel for trapping particles to be fed to said channel with a fluid.
DE102015219608B4 (de) 2015-10-09 2018-05-03 Clariant International Ltd Universelle Pigmentdispersionen auf Basis von N-Alkylglukaminen
DE102015219651A1 (de) 2015-10-09 2017-04-13 Clariant International Ltd. Zusammensetzungen enthaltend Zuckeramin und Fettsäure
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
DE102016207877A1 (de) 2016-05-09 2017-11-09 Clariant International Ltd Stabilisatoren für Silikatfarben
IL263274B2 (en) 2016-05-26 2023-10-01 Berkeley Lights Inc Covalently adapted surfaces, kits and methods for their production and uses
DE102016210164A1 (de) 2016-06-08 2017-12-14 Clariant International Ltd Verwendung von N-substituierten Pyrrolidonen zur Förderung der Penetration von agrochemischen Wirkstoffen
CN109715781A (zh) 2016-08-22 2019-05-03 米罗库鲁斯公司 用于数字微流控设备中的并行液滴控制的反馈系统
EP3516401B1 (en) 2016-09-19 2025-07-30 Roche Diagnostics GmbH Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
CN110383061A (zh) 2016-12-28 2019-10-25 米罗库鲁斯公司 数字微流控设备和方法
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN110945139B (zh) 2017-05-18 2023-09-05 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10730048B2 (en) * 2017-06-21 2020-08-04 Sharp Life Science (Eu) Limited EWOD device with holdback feature for fluid loading
EP3658908B1 (en) 2017-07-24 2025-11-12 Integra Biosciences AG Digital microfluidics systems and methods with integrated plasma collection device
US20190064173A1 (en) 2017-08-22 2019-02-28 10X Genomics, Inc. Methods of producing droplets including a particle and an analyte
CA3073058A1 (en) 2017-09-01 2019-03-07 Miroculus Inc. Digital microfluidics devices and methods of using them
US20190110472A1 (en) 2017-10-12 2019-04-18 Clariant International, Ltd. Active ingredient compositions comprising n-alkenoyl-n-alkylglucamides and the use thereof
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019226919A1 (en) 2018-05-23 2019-11-28 Miroculus Inc. Control of evaporation in digital microfluidics
CA3215214A1 (en) * 2018-09-06 2020-03-06 Nicoya Lifesciences Inc. Plasmon resonance (pr) system and instrument, digital microfluidic (dmf) cartridge, and methods of using localized surface plasmon resonance (lspr) for analysis of analytes
GB2577607B (en) * 2018-09-28 2023-05-17 Guangdong Acxel Micro & Nano Tech Co Ltd Droplet actuation
US10913067B2 (en) * 2018-10-01 2021-02-09 Sharp Life Science (Eu) Limited Barrier droplet configurations against migration between droplets on AM-EWOD devices
EP3917670A4 (en) 2019-01-31 2022-11-02 Miroculus Inc. ANTIFOULING COMPOSITIONS AND METHODS FOR HANDLING AND TREATING ENCAPSULATED DROPLET
WO2020176882A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
US12582991B2 (en) 2019-02-28 2026-03-24 Integra Biosciences Ag Digital microfluidics devices and methods of using them
CN109999929B (zh) * 2019-03-28 2021-04-30 上海天马微电子有限公司 微流控装置及其驱动方法
CN114206499B (zh) 2019-04-08 2024-10-08 米罗库鲁斯公司 多盒式数字微流控装置和使用方法
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
US12059679B2 (en) 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
WO2021252923A1 (en) 2020-06-12 2021-12-16 Genmark Diagnostics, Inc. Systems and methods for assessing bays on diagnostic devices
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
CN118201982A (zh) 2022-08-18 2024-06-14 10X基因组学有限公司 具有含氟二醇添加剂的液滴形成装置和方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060054503A1 (en) 2002-09-24 2006-03-16 Duke University Methods for manipulating droplets by electrowetting-based techniques
JP2007093605A (ja) 2005-09-28 2007-04-12 Samsung Electronics Co Ltd エレクトロウェッティングにおける液滴の接触角変位及び変化速度の増加方法及びその方法により形成された液滴を適用した液滴制御装置
US20070242111A1 (en) 2006-04-18 2007-10-18 Pamula Vamsee K Droplet-based diagnostics

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4849340A (en) * 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay
JP3791999B2 (ja) * 1997-03-24 2006-06-28 株式会社アドバンス 液体微粒子ハンドリング装置
US6565727B1 (en) * 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6485690B1 (en) * 1999-05-27 2002-11-26 Orchid Biosciences, Inc. Multiple fluid sample processor and system
EP1094318B1 (en) * 1999-10-22 2007-03-28 Ngk Insulators, Ltd. DNA chip and method for producing the same
CN1204267C (zh) * 2000-05-05 2005-06-01 朱学军 一种检测细胞内核酸的方法和装置
US6773566B2 (en) * 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
CN1325909C (zh) * 2000-09-27 2007-07-11 清华大学 用于微粒操纵与微粒导向的装置及其使用方法
FR2817343B1 (fr) * 2000-11-29 2003-05-09 Commissariat Energie Atomique Procede et dispositifs de transport et de concentration d'un analyte present dans un echantillon
JP3778041B2 (ja) * 2000-12-08 2006-05-24 コニカミノルタホールディングス株式会社 粒子分離機構及び粒子分離装置
JP3805746B2 (ja) * 2001-02-23 2006-08-09 独立行政法人科学技術振興機構 液体微粒子のハンドリング方法およびその装置
JP3673854B2 (ja) * 2001-05-11 2005-07-20 独立行政法人産業技術総合研究所 口径可変式吸引ノズルを備えたダスト試料採取装置
US20020168297A1 (en) * 2001-05-11 2002-11-14 Igor Shvets Method and device for dispensing of droplets
WO2002093245A1 (en) * 2001-05-15 2002-11-21 E Ink Corporation Electrophoretic displays containing magnetic particles
JP2003084123A (ja) * 2001-06-29 2003-03-19 Seiko Epson Corp カラーフィルタ基板、カラーフィルタ基板の製造方法、液晶表示装置、電気光学装置、電気光学装置の製造方法及び電子機器
US6705716B2 (en) * 2001-10-11 2004-03-16 Hewlett-Packard Development Company, L.P. Thermal ink jet printer for printing an image on a receiver and method of assembling the printer
AU2002359508A1 (en) * 2001-11-26 2003-06-10 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
FR2841063B1 (fr) * 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
JP4031322B2 (ja) * 2002-08-26 2008-01-09 独立行政法人科学技術振興機構 液滴操作装置
WO2004027379A2 (en) * 2002-09-20 2004-04-01 Novus Molecular, Inc. Methods and devices for active bioassay
US7547380B2 (en) * 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
DE10311622B4 (de) * 2003-03-17 2005-06-09 Disetronic Licensing Ag Sensorisches Membran-Osmometer und osmotisches Messverfahren zur quantitativen Bestimmung niedermolekularer Affinitätsliganden
EP2305173B1 (en) * 2003-03-28 2016-05-11 Inguran, LLC Apparatus and methods for providing sorted particles
TWI230760B (en) * 2003-07-29 2005-04-11 Univ Tsinghua Electrowetting electrode design with electromagnetic field for actuation of the magnetic-beads biochemical detection system
CA2479452C (en) * 2003-08-30 2008-11-04 F.Hoffmann-La Roche Ag Method and device for determining analytes in a liquid
US7470397B2 (en) * 2003-10-24 2008-12-30 Adhesives Research, Inc. Disintegratable films for diagnostic devices
DE10352535A1 (de) 2003-11-07 2005-06-16 Steag Microparts Gmbh Mikrostrukturierte Trennvorrichtung und Verfahren zum Abtrennen von flüssigen Bestandteilen aus einer Partikel enthaltenden Flüssigkeit
CN100478075C (zh) * 2003-11-17 2009-04-15 皇家飞利浦电子股份有限公司 用于操纵流体实体的系统
CA2553025A1 (en) * 2004-01-14 2005-07-28 Luminex Corporation Methods and systems for dynamic range expansion
US20080226500A1 (en) * 2004-01-15 2008-09-18 Mitsuhiro Shikida Chemical Analytic Apparatus and Chemical Analytic Method
JP4068581B2 (ja) * 2004-03-08 2008-03-26 独立行政法人科学技術振興機構 ナノ空隙制御による微粒子のサイズ分離分析法およびその装置
JP4486383B2 (ja) * 2004-03-08 2010-06-23 株式会社アドバンテスト パターン発生器、及び試験装置
FR2872715B1 (fr) * 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) * 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
CN2735342Y (zh) * 2004-10-20 2005-10-19 清华大学 一种介质层上的电润湿微液滴驱动器
DE102004059280B4 (de) * 2004-12-09 2007-08-16 Dräger Safety AG & Co. KGaA Elektrochemischer Gassensor
US7458661B2 (en) * 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
EP1859330B1 (en) * 2005-01-28 2012-07-04 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
FR2884437B1 (fr) * 2005-04-19 2007-07-20 Commissariat Energie Atomique Dispositif et procede microfluidique de transfert de matiere entre deux phases immiscibles.
CA2606750C (en) 2005-05-11 2015-11-24 Nanolytics, Inc. Method and device for conducting biochemical or chemical reactions at multiple temperatures
JP2006329904A (ja) * 2005-05-30 2006-12-07 Hitachi High-Technologies Corp 液体搬送デバイス及び分析システム
JP4500733B2 (ja) * 2005-05-30 2010-07-14 株式会社日立ハイテクノロジーズ 化学分析装置
US20070023292A1 (en) * 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
CN102622746B (zh) * 2005-09-21 2016-05-25 卢米尼克斯股份有限公司 图像数据处理的方法和系统
US7344679B2 (en) * 2005-10-14 2008-03-18 International Business Machines Corporation Method and apparatus for point of care osmolarity testing
CN101351270A (zh) * 2005-10-22 2009-01-21 精华微技有限公司 从用于芯片微流控的液柱中抽取液滴
US7365022B2 (en) * 2006-01-20 2008-04-29 Palo Alto Research Center Incorporated Additive printed mask process and structures produced thereby
US20070207513A1 (en) * 2006-03-03 2007-09-06 Luminex Corporation Methods, Products, and Kits for Identifying an Analyte in a Sample
US7439014B2 (en) * 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
WO2010027894A2 (en) 2008-08-27 2010-03-11 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US7901947B2 (en) * 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
EP2016189B1 (en) 2006-04-18 2012-01-25 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
CN101490562B (zh) * 2006-07-10 2012-12-19 株式会社日立高新技术 液体输送设备
US9266076B2 (en) * 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
US8202491B2 (en) * 2006-11-21 2012-06-19 Bioscale, Inc. Apparatus for analyte processing
EP2857526B1 (en) * 2006-12-13 2016-08-17 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
EP2573562A3 (en) 2007-02-09 2013-10-30 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
EP2109774B1 (en) 2007-02-15 2018-07-04 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
US8093062B2 (en) * 2007-03-22 2012-01-10 Theodore Winger Enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil
JP2010524002A (ja) * 2007-04-10 2010-07-15 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴分配装置および方法
WO2008134153A1 (en) 2007-04-23 2008-11-06 Advanced Liquid Logic, Inc. Bead-based multiplexed analytical methods and instrumentation
US20080283414A1 (en) * 2007-05-17 2008-11-20 Monroe Charles W Electrowetting devices
WO2009003184A1 (en) 2007-06-27 2008-12-31 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
WO2009021173A1 (en) 2007-08-08 2009-02-12 Advanced Liquid Logic, Inc. Use of additives for enhancing droplet operations
ES2438989T3 (es) * 2008-05-13 2014-01-21 Advanced Liquid Logic, Inc. Dispositivos, sistemas y métodos accionadores de gotitas
US8093064B2 (en) * 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060054503A1 (en) 2002-09-24 2006-03-16 Duke University Methods for manipulating droplets by electrowetting-based techniques
JP2007093605A (ja) 2005-09-28 2007-04-12 Samsung Electronics Co Ltd エレクトロウェッティングにおける液滴の接触角変位及び変化速度の増加方法及びその方法により形成された液滴を適用した液滴制御装置
US20070242111A1 (en) 2006-04-18 2007-10-18 Pamula Vamsee K Droplet-based diagnostics

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EP2188059A4 (en) 2015-05-20
AU2008293652A1 (en) 2009-03-05
US20110086377A1 (en) 2011-04-14
CN101808751A (zh) 2010-08-18
AU2008293652B2 (en) 2013-02-21
KR20100133943A (ko) 2010-12-22
WO2009029561A3 (en) 2009-05-22
US8591830B2 (en) 2013-11-26
JP5302966B2 (ja) 2013-10-02
BRPI0815698A2 (pt) 2017-06-13
WO2009029561A2 (en) 2009-03-05
JP2013242321A (ja) 2013-12-05
US20140174933A1 (en) 2014-06-26
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