KR101439110B1 - 이염기성 ｂ쇄 말단을 갖는 인슐린 유사체를 제조하는 방법 - Google Patents

이염기성 ｂ쇄 말단을 갖는 인슐린 유사체를 제조하는 방법 Download PDF

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Publication number
KR101439110B1
KR101439110B1 KR1020097000429A KR20097000429A KR101439110B1 KR 101439110 B1 KR101439110 B1 KR 101439110B1 KR 1020097000429 A KR1020097000429 A KR 1020097000429A KR 20097000429 A KR20097000429 A KR 20097000429A KR 101439110 B1 KR101439110 B1 KR 101439110B1
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KR
South Korea
Prior art keywords
insulin
amino acid
arg
trypsin
chain
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Expired - Fee Related
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KR1020097000429A
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English (en)
Korean (ko)
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KR20090038868A (ko
Inventor
파울 하버만
프랑크 조허
Original Assignee
사노피-아벤티스 도이칠란트 게엠베하
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Diabetes (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Emergency Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020097000429A 2006-07-11 2007-07-05 이염기성 ｂ쇄 말단을 갖는 인슐린 유사체를 제조하는 방법 Expired - Fee Related KR101439110B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102006031955.9 2006-07-11
DE102006031955A DE102006031955A1 (de) 2006-07-11 2006-07-11 Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende
PCT/EP2007/005933 WO2008006497A1 (de) 2006-07-11 2007-07-05 Verfahren zur herstellung von insulinanaloga mit dibasischem b-kettenende

Publications (2)

Publication Number Publication Date
KR20090038868A KR20090038868A (ko) 2009-04-21
KR101439110B1 true KR101439110B1 (ko) 2014-09-12

Family

ID=38468893

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020097000429A Expired - Fee Related KR101439110B1 (ko) 2006-07-11 2007-07-05 이염기성 ｂ쇄 말단을 갖는 인슐린 유사체를 제조하는 방법

Country Status (19)

Country Link
US (1) US8410048B2 (enExample)
EP (1) EP2041170B1 (enExample)
JP (1) JP5331685B2 (enExample)
KR (1) KR101439110B1 (enExample)
CN (1) CN101490082B (enExample)
AR (1) AR061865A1 (enExample)
AU (1) AU2007272057B2 (enExample)
BR (1) BRPI0714297A2 (enExample)
CA (1) CA2657041C (enExample)
DE (1) DE102006031955A1 (enExample)
DK (1) DK2041170T3 (enExample)
ES (1) ES2423684T3 (enExample)
IL (1) IL196360A (enExample)
MX (1) MX2009000275A (enExample)
MY (1) MY148984A (enExample)
NO (1) NO20090663L (enExample)
PT (1) PT2041170E (enExample)
TW (1) TWI440717B (enExample)
WO (1) WO2008006497A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
CA2705821A1 (en) 2007-11-16 2009-05-22 Novo Nordisk A/S Pharmaceutical compositions containing insulin and an insulinotropic peptide
MY161892A (en) * 2008-02-19 2017-05-15 Biocon Ltd A method of obtaining a purified, biologically active heterologous protein
WO2012015692A2 (en) * 2010-07-28 2012-02-02 Smartcells, Inc. Recombinantly expressed insulin polypeptides and uses thereof
WO2013015697A1 (en) 2011-07-28 2013-01-31 Mabion S.A. A recombinant protein, a polynucleotide encoding it as well as a method of obtaining insulin or its an analogue
CN102504022A (zh) * 2011-11-30 2012-06-20 苏州元基生物技术有限公司 含有保护赖氨酸的胰岛素原及使用其制备胰岛素的方法
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5015728A (en) * 1983-09-17 1991-05-14 Hoechst Aktiengesellschaft Process for the preparation of insulin derivatives, the B chain of which is lengthened c-terminally

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55138393A (en) * 1979-04-13 1980-10-29 Shionogi & Co Ltd Semisynthesis of insulin
DE3326473A1 (de) 1983-07-22 1985-01-31 Hoechst Ag, 6230 Frankfurt Pharmazeutisches mittel zur behandlung des diabetes mellitus
DK113585D0 (da) * 1985-03-12 1985-03-12 Novo Industri As Nye peptider
DK347086D0 (da) * 1986-07-21 1986-07-21 Novo Industri As Novel peptides
PH25772A (en) * 1985-08-30 1991-10-18 Novo Industri As Insulin analogues, process for their preparation
DE3636903A1 (de) 1985-12-21 1987-07-02 Hoechst Ag Fusionsproteine mit eukaryotischem ballastanteil
GB8618152D0 (en) * 1986-07-25 1986-09-03 Renishaw Plc Co-ordinate measuring
EP0294851A3 (de) * 1987-06-12 1990-05-09 Berlin-Chemie Ag Verfahren zur Herstellung von Humaninsulin und seinen Derivaten
US6875589B1 (en) * 1988-06-23 2005-04-05 Hoechst Aktiengesellschaft Mini-proinsulin, its preparation and use
DE3837825A1 (de) * 1988-11-08 1990-05-10 Hoechst Ag Neue insulinderivate, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
DE3844211A1 (de) 1988-12-29 1990-07-05 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
US5227293A (en) 1989-08-29 1993-07-13 The General Hospital Corporation Fusion proteins, their preparation and use
DE4405179A1 (de) 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
EP1076066A1 (en) 1999-07-12 2001-02-14 Zealand Pharmaceuticals A/S Peptides for lowering blood glucose levels
AR025646A1 (es) * 2000-09-13 2002-12-04 Beta Lab Sa Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
DE10108211A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
DE10108100A1 (de) 2001-02-20 2002-08-29 Aventis Pharma Gmbh Verwendung supersekretierbarer Peptide in Verfahren zu deren Herstellung und paralleler Verbesserung der Exportate eines oder mehrerer anderer Polypeptide von Interesse
DE10108212A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
IL161358A0 (en) * 2001-11-19 2004-09-27 Novo Nordisk As Process for preparing insulin compounds
DE10227232A1 (de) 2002-06-18 2004-01-15 Aventis Pharma Deutschland Gmbh Saure Insulinzubereitungen mit verbesserter Stabilität
ES2327328T3 (es) 2002-07-04 2009-10-28 Zealand Pharma A/S Glp-1 y procedimientos para el tratamiento de la diabetes.
EP1778839B1 (en) 2004-08-13 2008-07-09 Roche Diagniostics GMBH C-terminal modification of polypeptides
DE102004058306A1 (de) 2004-12-01 2006-07-27 Sanofi-Aventis Deutschland Gmbh Verfahren zur Herstellung von Carboxy-terminal amidierten Peptiden
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5015728A (en) * 1983-09-17 1991-05-14 Hoechst Aktiengesellschaft Process for the preparation of insulin derivatives, the B chain of which is lengthened c-terminally

Also Published As

Publication number Publication date
CN101490082A (zh) 2009-07-22
ES2423684T3 (es) 2013-09-23
AU2007272057B2 (en) 2012-06-14
AR061865A1 (es) 2008-09-24
WO2008006497A1 (de) 2008-01-17
PT2041170E (pt) 2013-07-18
DE102006031955A1 (de) 2008-01-17
NO20090663L (no) 2009-02-11
BRPI0714297A2 (pt) 2013-02-26
TWI440717B (zh) 2014-06-11
IL196360A (en) 2015-08-31
EP2041170B1 (de) 2013-05-01
US20090192073A1 (en) 2009-07-30
JP2009542235A (ja) 2009-12-03
EP2041170A1 (de) 2009-04-01
JP5331685B2 (ja) 2013-10-30
MY148984A (en) 2013-06-28
MX2009000275A (es) 2009-01-26
HK1130815A1 (en) 2010-01-08
DK2041170T3 (da) 2013-08-05
CA2657041A1 (en) 2008-01-17
AU2007272057A1 (en) 2008-01-17
IL196360A0 (en) 2011-08-01
TW200817511A (en) 2008-04-16
CN101490082B (zh) 2013-08-14
KR20090038868A (ko) 2009-04-21
CA2657041C (en) 2016-04-26
US8410048B2 (en) 2013-04-02

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