CN101490082B - 产生具有二碱基b链末端的胰岛素类似物的方法 - Google Patents

产生具有二碱基b链末端的胰岛素类似物的方法 Download PDF

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Publication number
CN101490082B
CN101490082B CN2007800261387A CN200780026138A CN101490082B CN 101490082 B CN101490082 B CN 101490082B CN 2007800261387 A CN2007800261387 A CN 2007800261387A CN 200780026138 A CN200780026138 A CN 200780026138A CN 101490082 B CN101490082 B CN 101490082B
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China
Prior art keywords
arg
gly
insulin
chain
insulin human
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Expired - Fee Related
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CN2007800261387A
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English (en)
Chinese (zh)
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CN101490082A (zh
Inventor
P·哈伯曼
F·措赫尔
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Sanofi Aventis Deutschland GmbH
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Sanofi Aventis Deutschland GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Diabetes (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Emergency Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN2007800261387A 2006-07-11 2007-07-05 产生具有二碱基b链末端的胰岛素类似物的方法 Expired - Fee Related CN101490082B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102006031955.9 2006-07-11
DE102006031955A DE102006031955A1 (de) 2006-07-11 2006-07-11 Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende
PCT/EP2007/005933 WO2008006497A1 (de) 2006-07-11 2007-07-05 Verfahren zur herstellung von insulinanaloga mit dibasischem b-kettenende

Publications (2)

Publication Number Publication Date
CN101490082A CN101490082A (zh) 2009-07-22
CN101490082B true CN101490082B (zh) 2013-08-14

Family

ID=38468893

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CN2007800261387A Expired - Fee Related CN101490082B (zh) 2006-07-11 2007-07-05 产生具有二碱基b链末端的胰岛素类似物的方法

Country Status (19)

Country Link
US (1) US8410048B2 (enExample)
EP (1) EP2041170B1 (enExample)
JP (1) JP5331685B2 (enExample)
KR (1) KR101439110B1 (enExample)
CN (1) CN101490082B (enExample)
AR (1) AR061865A1 (enExample)
AU (1) AU2007272057B2 (enExample)
BR (1) BRPI0714297A2 (enExample)
CA (1) CA2657041C (enExample)
DE (1) DE102006031955A1 (enExample)
DK (1) DK2041170T3 (enExample)
ES (1) ES2423684T3 (enExample)
IL (1) IL196360A (enExample)
MX (1) MX2009000275A (enExample)
MY (1) MY148984A (enExample)
NO (1) NO20090663L (enExample)
PT (1) PT2041170E (enExample)
TW (1) TWI440717B (enExample)
WO (1) WO2008006497A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen
CA2705821A1 (en) 2007-11-16 2009-05-22 Novo Nordisk A/S Pharmaceutical compositions containing insulin and an insulinotropic peptide
MY161892A (en) * 2008-02-19 2017-05-15 Biocon Ltd A method of obtaining a purified, biologically active heterologous protein
WO2012015692A2 (en) * 2010-07-28 2012-02-02 Smartcells, Inc. Recombinantly expressed insulin polypeptides and uses thereof
WO2013015697A1 (en) 2011-07-28 2013-01-31 Mabion S.A. A recombinant protein, a polynucleotide encoding it as well as a method of obtaining insulin or its an analogue
CN102504022A (zh) * 2011-11-30 2012-06-20 苏州元基生物技术有限公司 含有保护赖氨酸的胰岛素原及使用其制备胰岛素的方法
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0140084A1 (de) * 1983-09-17 1985-05-08 Hoechst Aktiengesellschaft Verfahren zur Herstellung von Insulin-Derivaten, deren B-Kette C-terminal verlängert ist, neue basisch modifizierte Insulin-Derivate, diese enthaltende Mittel und ihre Verwendung
EP0254515A2 (en) * 1986-07-25 1988-01-27 Renishaw plc Co-ordinate measuring

Family Cites Families (23)

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Publication number Priority date Publication date Assignee Title
JPS55138393A (en) * 1979-04-13 1980-10-29 Shionogi & Co Ltd Semisynthesis of insulin
DE3326473A1 (de) 1983-07-22 1985-01-31 Hoechst Ag, 6230 Frankfurt Pharmazeutisches mittel zur behandlung des diabetes mellitus
DK113585D0 (da) * 1985-03-12 1985-03-12 Novo Industri As Nye peptider
DK347086D0 (da) * 1986-07-21 1986-07-21 Novo Industri As Novel peptides
PH25772A (en) * 1985-08-30 1991-10-18 Novo Industri As Insulin analogues, process for their preparation
DE3636903A1 (de) 1985-12-21 1987-07-02 Hoechst Ag Fusionsproteine mit eukaryotischem ballastanteil
EP0294851A3 (de) * 1987-06-12 1990-05-09 Berlin-Chemie Ag Verfahren zur Herstellung von Humaninsulin und seinen Derivaten
US6875589B1 (en) * 1988-06-23 2005-04-05 Hoechst Aktiengesellschaft Mini-proinsulin, its preparation and use
DE3837825A1 (de) * 1988-11-08 1990-05-10 Hoechst Ag Neue insulinderivate, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
DE3844211A1 (de) 1988-12-29 1990-07-05 Hoechst Ag Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
US5227293A (en) 1989-08-29 1993-07-13 The General Hospital Corporation Fusion proteins, their preparation and use
DE4405179A1 (de) 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
EP1076066A1 (en) 1999-07-12 2001-02-14 Zealand Pharmaceuticals A/S Peptides for lowering blood glucose levels
AR025646A1 (es) * 2000-09-13 2002-12-04 Beta Lab Sa Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
DE10108211A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
DE10108100A1 (de) 2001-02-20 2002-08-29 Aventis Pharma Gmbh Verwendung supersekretierbarer Peptide in Verfahren zu deren Herstellung und paralleler Verbesserung der Exportate eines oder mehrerer anderer Polypeptide von Interesse
DE10108212A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
IL161358A0 (en) * 2001-11-19 2004-09-27 Novo Nordisk As Process for preparing insulin compounds
DE10227232A1 (de) 2002-06-18 2004-01-15 Aventis Pharma Deutschland Gmbh Saure Insulinzubereitungen mit verbesserter Stabilität
ES2327328T3 (es) 2002-07-04 2009-10-28 Zealand Pharma A/S Glp-1 y procedimientos para el tratamiento de la diabetes.
EP1778839B1 (en) 2004-08-13 2008-07-09 Roche Diagniostics GMBH C-terminal modification of polypeptides
DE102004058306A1 (de) 2004-12-01 2006-07-27 Sanofi-Aventis Deutschland Gmbh Verfahren zur Herstellung von Carboxy-terminal amidierten Peptiden
DE102005046113A1 (de) * 2005-09-27 2007-03-29 Sanofi-Aventis Deutschland Gmbh Verfahren zur Amidierung von Polypetiden mit C-terminalen basischen Aminosäuren unter Verwendung spezifischer Endoproteasen

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0140084A1 (de) * 1983-09-17 1985-05-08 Hoechst Aktiengesellschaft Verfahren zur Herstellung von Insulin-Derivaten, deren B-Kette C-terminal verlängert ist, neue basisch modifizierte Insulin-Derivate, diese enthaltende Mittel und ihre Verwendung
EP0254515A2 (en) * 1986-07-25 1988-01-27 Renishaw plc Co-ordinate measuring

Also Published As

Publication number Publication date
CN101490082A (zh) 2009-07-22
ES2423684T3 (es) 2013-09-23
AU2007272057B2 (en) 2012-06-14
AR061865A1 (es) 2008-09-24
WO2008006497A1 (de) 2008-01-17
PT2041170E (pt) 2013-07-18
DE102006031955A1 (de) 2008-01-17
NO20090663L (no) 2009-02-11
BRPI0714297A2 (pt) 2013-02-26
TWI440717B (zh) 2014-06-11
IL196360A (en) 2015-08-31
EP2041170B1 (de) 2013-05-01
US20090192073A1 (en) 2009-07-30
JP2009542235A (ja) 2009-12-03
EP2041170A1 (de) 2009-04-01
JP5331685B2 (ja) 2013-10-30
KR101439110B1 (ko) 2014-09-12
MY148984A (en) 2013-06-28
MX2009000275A (es) 2009-01-26
HK1130815A1 (en) 2010-01-08
DK2041170T3 (da) 2013-08-05
CA2657041A1 (en) 2008-01-17
AU2007272057A1 (en) 2008-01-17
IL196360A0 (en) 2011-08-01
TW200817511A (en) 2008-04-16
KR20090038868A (ko) 2009-04-21
CA2657041C (en) 2016-04-26
US8410048B2 (en) 2013-04-02

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