KR101162226B1 - P ef-tu 발현 유니트 - Google Patents
P ef-tu 발현 유니트 Download PDFInfo
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- KR101162226B1 KR101162226B1 KR1020067014165A KR20067014165A KR101162226B1 KR 101162226 B1 KR101162226 B1 KR 101162226B1 KR 1020067014165 A KR1020067014165 A KR 1020067014165A KR 20067014165 A KR20067014165 A KR 20067014165A KR 101162226 B1 KR101162226 B1 KR 101162226B1
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
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- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
Description
단백질 | 단백질 코딩 핵산 | 참조 문헌 | 참조 문헌에서 서열 |
아스파르테이트 키나제 | ask 또는 lysC | EP1108790 | DNA: 281 단백질: 3781 |
아스파르테이트-세미알데히드 데히드로게나제 | asd | EP1108790 | DNA: 331 단백질: 3831 |
디히드로디피콜리네이트 합성효소 | dapA | WO 0100843 | DNA: 55 단백질: 56 |
디히드로디피콜리네이트 리덕타제 | dapB | WO 0100843 | DNA: 35 단백질: 36 |
메조-디아미노피멜레이트 D-데히드로게나제 | ddh | EP1108790 | DNA: 3494 단백질: 6944 |
디아미노피콜리네이트 데카르복실라제 | lysA | EP1108790 | DNA: 3451 단백질: 6951 |
라이신 엑스포터 | lysE | EP1108790 | DNA: 3455 단백질: 6955 |
아르기닐-tRNA 합성효소 | argS | EP1108790 | DNA: 3450 단백질: 6950 |
글루코스-6-포스페이트 데히드로게나제 | zwf | WO 0100844 | DNA: 243 단백질: 244 |
글리세르알데히드-3-포스페이트 데히드로게나제 | gap | WO 0100844 | DNA: 187 단백질: 188 |
3-포스포글리세레이트 키나제 | pgk | WO 0100844 | DNA: 69 단백질: 70 |
피루베이트 카르복실라제 | pycA | EP1108790 | DNA: 765 단백질: 4265 |
트리오스포스페이트 이소머라제 | tpi | WO 0100844 | DNA: 61 단백질: 62 |
비오틴 리가제 | birA | EP1108790 | DNA: 786 단백질: 4286 |
PEP 카르복실라제 | pck | EP1108790 | DNA: 3470 단백질: 6970 |
호모세린 키나제 | thrB | WO 0100843 | DNA: 173 단백질: 174 |
트레오닌 신타제 | thrC | WO 0100843 | DNA: 175 단백질: 176 |
트레오닌 엑스포트 캐리어 | thrE | WO 0251231 | DNA: 41 단백질: 42 |
트레오닌 유출 단백질(efflux protein) | RXA2390 | WO 0100843 | DNA: 7 단백질: 8 |
트레오닌 데히드라타제 | ilvA | EP 1108790 | DNA: 2328 단백질: 5828 |
호모세린 O-아세틸트랜스퍼라제 | metA | EP 1108790 | DNA: 727 단백질: 4227 |
시스타티오닌 감마-신타제 | metB | EP 1108790 | DNA: 3491 단백질: 6991 |
시스타티오닌 베타-리아제 | metC | EP 1108790 | DNA: 2535 단백질: 6035 |
조효소 B12-의존성 메티오닌 신타제 | metH | EP 1108790 | DNA: 1663 단백질: 5163 |
O-아세틸호모세린 술프히드릴라제 | metY | EP 1108790 | DNA: 726 단백질: 4226 |
메틸렌테트라히드로폴레이트 리덕타제 | metF | EP 1108790 | DNA: 2379 단백질: 5879 |
D-3-포스포글리세레이트 데히드로게나제 | serA | EP 1108790 | DNA: 1415 단백질: 4915 |
포스포세린 포스파타제 1 | serB | WO 0100843 | DNA: 153 단백질: 154 |
포스포세린 포스파타제 2 | serB | EP 1108790 | DNA: 467 단백질: 3967 |
포스포세린 포스파타제 3 | serB | EP 1108790 | DNA: 334 단백질: 3834 |
포스포세린 아미노트랜스퍼라제 | serC | WO 0100843 | DNA: 151 단백질: 152 |
세린 아세틸트랜스퍼라제 | cysE | WO 0100843 | DNA: 243 단백질: 244 |
시스테인 신타제 I | cysK | EP 1108790 | DNA: 2817 단백질: 6317 |
시스테인 신타제 II | CysM | EP 1108790 | DNA: 2338 단백질: 5838 |
호모세린 데히드로게나제 | hom | EP 1108790 | DNA: 3452 단백질: 6952 |
조효소 B12-비의존성 메티오닌 신타제 | metE | WO 0100843 | DNA: 755 단백질: 756 |
세린 히드록시메틸트랜스퍼라제 | glyA | WO 0100843 | DNA: 143 단백질: 144 |
술페이트 환원 단백질 | RXA247 | EP 1108790 | DNA: 3089 단백질: 6589 |
술페이트 환원 단백질 | RXA248 | EP 1108790 | DNA: 3090 단백질: 6590 |
술페이트 아데닐일트랜스퍼라제 서브유닛 1 | CysN | EP 1108790 | DNA: 3092 단백질: 6592 |
술페이트 아데닐일트랜스퍼라제 서브유닛 2 | CysD | EP 1108790 | DNA: 3093 단백질: 6593 |
포스포아데노신 포스포술페이트 리덕타제 |
CysH | WO 02729029 | DNA: 7 단백질:8 |
페레독신-술파이트 리덕타제 | RXA073 | WO 0100842 | DNA: 329 단백질: 330 |
페레독신 NADP 리덕타제 | RXA076 | WO 0100843 | DNA: 79 단백질: 80 |
전사 레귤레이터 LuxR | luxR | WO 0100842 | DNA: 297 단백질: 298 |
전사 레귤레이터 LysR1 | lysR1 | EP 1108790 | DNA: 676 단백질: 4176 |
전사 레귤레이터 LysR2 | lysR2 | EP 1108790 | DNA: 3228 단백질: 6728 |
전사 레귤레이터 LysR3 | lysR3 | EP 1108790 | DNA: 2200 단백질: 5700 |
말레이트-퀴논 옥시도리덕타제 | mqo | WO 0100844 | DNA: 569 단백질: 570 |
트랜스케톨라제 | RXA2739 | EP 1108790 | DNA: 1740 단백질: 5240 |
트랜스알돌라제 | RXA2738 | WO 0100844 | DNA: 245 단백질: 246 |
OpcA | OpcA | WO 0100804 | DNA: 79 단백질: 80 |
1-포스포프럭토키나제 1 | pfk1 | WO 0100844 | DNA: 55 단백질: 56 |
1-포스포프럭토키나제 2 | pfk2 | WO 0100844 | DNA: 57 단백질: 58 |
6-포스포프럭토키나제 1 | 6-pfk1 | EP 1108790 | DNA: 1383 단백질: 4883 |
6-포스포프럭토키나제 2 | 6-pfk2 | DE 10112992 | DNA: 1 단백질: 2 |
프럭토스-1,6-비스포스파타제 1 | fbr1 | EP 1108790 | DNA: 1136 단백질: 4636 |
피루베이트 옥시다제 | poxB | WO 0100844 | DNA: 85 단백질: 86 |
RXA00655 레귤레이터 | RXA655 | US2003162267.2 | DNA: 1 단백질: 2 |
RXN02910 레귤레이터 | RXN2910 | US2003162267.2 | DNA: 5 단백질: 6 |
6-포스포글루코노락토나제 | RXA2735 | WO 0100844 | DNA: 1 단백질: 2 |
컬럼 1 | 컬럼 2 | 컬럼 3 | 컬럼 4 | 컬럼 5 |
ID | AA 위치 | AA 야생형 | AA 돌연변이체 | 기능 |
ask | 317 | S | A | 아스파르테이트 키나제 |
311 | T | I | ||
279 | A | T | ||
asd | 66 | D | G | 아스파르테이트-세미알데히드 데히드로게나제 |
234 | R | H | ||
272 | D | E | ||
285 | K | E | ||
20 | L | F | ||
dapA | 2 | S | A | 디히드로디피콜리네이트 합성효소 |
84 | K | N | ||
85 | L | V | ||
dapB | 91 | D | A | 디히드로디피콜리네이트 리덕타제 |
83 | D | N | ||
ddh | 174 | D | E | 메조-디아미노피멜레이트 D-데히드로게나제 |
235 | F | L | ||
237 | S | A | ||
lysA | 265 | A | D | 디아미노피콜리네이트 데카르복실라제 |
320 | D | N | ||
332 | I | V | ||
argS | 355 | G | D | 아르기닐-tRNA 합성효소 |
156 | A | S | ||
513 | V | A | ||
540 | H | R | ||
zwf | 8 | S | T | 글루코스-6-포스페이트 데히드로게나제 |
150 | T | A | ||
321 | G | S |
gap | 264 | G | S | 글리세르알데히드-3-포스페이트 데히드로게나제 |
pycA | 7 | S | L | 피루베이트 카르복실라제 |
153 | E | D | ||
182 | A | S | ||
206 | A | S | ||
227 | H | R | ||
455 | A | G | ||
458 | P | S | ||
639 | S | T | ||
1008 | R | H | ||
1059 | S | P | ||
1120 | D | E | ||
pck | 162 | H | Y | PEP 카르복실라제 |
241 | G | D | ||
829 | T | R | ||
thrB | 103 | S | A | 호모세린 키나제 |
190 | T | A | ||
133 | A | V | ||
138 | P | S | ||
thrC | 69 | G | R | 트레오닌 신타제 |
478 | T | I | ||
RXA330 | 85 | I | M | 트레오닌 유출 단백질 |
161 | F | I | ||
195 | G | D | ||
hom | 104 | V | I | 호모세린 데히드로게나제 |
116 | T | I | ||
148 | G | A | ||
59 | V | A | ||
270 | T | S | ||
345 | R | P | ||
268 | K | N | ||
61 | D | H | ||
72 | E | Q | ||
lysR1 | 80 | R | H | 전사 레귤레이터 LysR1 |
lysR3 | 142 | R | W | 전사 레귤레이터 LysR3 |
179 | A | T | ||
RXA2739 | 75 | N | D | 트랜스케톨라제 |
329 | A | T | ||
332 | A | T | ||
556 | V | I | ||
RXA2738 | 242 | K | M | 트랜스알돌라제 |
OpcA | 107 | Y | H | OpcA |
219 | K | N | ||
233 | P | S | ||
261 | Y | H | ||
312 | S | F | ||
65 | G | R | 아스파르테이트-1-데카르복실라제 | |
33 | G | S | 6-포스포글루코노락토나제 |
세균 | 기탁 번호 | ||||||||
속 | 종 | ATCC | FERM | NRRL | CECT | NCIMB | CBS | NCTC | DSMZ |
브레비박테리움 | 암모니아제네스 (ammoniagenes) | 21054 | |||||||
19350 | |||||||||
19351 | |||||||||
19352 | |||||||||
19353 | |||||||||
19354 | |||||||||
19355 | |||||||||
19356 | |||||||||
21055 | |||||||||
21077 | |||||||||
21553 | |||||||||
21580 | |||||||||
39101 | |||||||||
부타니쿰 (butanicum) | 21196 | ||||||||
디바리카툼 | 21792 | P928 | |||||||
플라붐 | 21474 | ||||||||
21129 | |||||||||
21518 | |||||||||
B11474 | |||||||||
B11472 | |||||||||
21127 | |||||||||
21128 | |||||||||
21427 | |||||||||
21475 | |||||||||
21517 | |||||||||
21528 | |||||||||
21529 | |||||||||
B11477 | |||||||||
B11478 | |||||||||
21127 | |||||||||
B11474 | |||||||||
헤알리이 (healii) | 15527 | ||||||||
케토글루타미쿰 (ketoglutamicum) | 21004 | ||||||||
21089 | |||||||||
케토소레덕툼 (ketosoreductum) | 21914 |
브레비박테리움 | 락토페르멘툼 | 70 | |||||||
74 | |||||||||
77 | |||||||||
21798 | |||||||||
21799 | |||||||||
21800 | |||||||||
21801 | |||||||||
B11470 | |||||||||
B11471 | |||||||||
21086 | |||||||||
21420 | |||||||||
21086 | |||||||||
31269 | |||||||||
리넨스 (linens) | 9174 | ||||||||
19391 | |||||||||
8377 | |||||||||
파라피놀리티쿰 (paraffinolyticum) | 11160 | ||||||||
스펙. (spec.) | 717.73 | ||||||||
717.73 | |||||||||
14604 | |||||||||
21860 | |||||||||
21864 | |||||||||
21865 | |||||||||
21866 | |||||||||
19240 | |||||||||
코리네박테리움 | 아세토아시도필룸 | 21476 | |||||||
13870 | |||||||||
아세토글루타미쿰 | B11473 | ||||||||
B11475 | |||||||||
15806 | |||||||||
21491 | |||||||||
31270 | |||||||||
아세토필룸 (acetophilum) | B3671 | ||||||||
암모니아제네스 | 6872 | 2399 | |||||||
15511 | |||||||||
푸지오켄세 (fujiokense) | 21496 | ||||||||
글루타미쿰 | 14067 | ||||||||
39137 | |||||||||
21254 | |||||||||
21255 | |||||||||
31830 | |||||||||
13032 |
코리네박테리움 | 글루타미쿰 | 14305 | |||||||
15455 | |||||||||
13058 | |||||||||
13059 | |||||||||
13060 | |||||||||
21492 | |||||||||
21513 | |||||||||
21526 | |||||||||
21543 | |||||||||
13287 | |||||||||
21851 | |||||||||
21253 | |||||||||
21514 | |||||||||
21516 | |||||||||
21299 | |||||||||
21300 | |||||||||
39684 | |||||||||
21488 | |||||||||
21649 | |||||||||
21650 | |||||||||
19223 | |||||||||
13869 | |||||||||
21157 | |||||||||
21158 | |||||||||
21159 | |||||||||
21355 | |||||||||
31808 | |||||||||
21674 | |||||||||
21562 | |||||||||
21563 | |||||||||
21564 | |||||||||
21565 | |||||||||
21566 | |||||||||
21567 | |||||||||
21568 | |||||||||
21569 | |||||||||
21570 | |||||||||
21571 | |||||||||
21572 | |||||||||
21573 | |||||||||
21579 | |||||||||
19049 |
코리네박테리움 | 글루타미쿰 | 19050 | |||||||
19051 | |||||||||
19052 | |||||||||
19053 | |||||||||
19054 | |||||||||
19055 | |||||||||
19056 | |||||||||
19057 | |||||||||
19058 | |||||||||
19059 | |||||||||
19060 | |||||||||
19185 | |||||||||
13286 | |||||||||
21515 | |||||||||
21527 | |||||||||
21544 | |||||||||
21492 | |||||||||
B8183 | |||||||||
B8182 | |||||||||
B12416 | |||||||||
B12417 | |||||||||
B12418 | |||||||||
B11476 | |||||||||
21608 | |||||||||
릴륨 (lilium) | P973 | ||||||||
니트릴로필루스 (nitrilophilus) | 21419 | 11594 | |||||||
스펙. | P4445 | ||||||||
P4446 | |||||||||
31088 | |||||||||
31089 | |||||||||
31090 | |||||||||
31090 | |||||||||
31090 | |||||||||
15954 | 20145 | ||||||||
21857 | |||||||||
21862 | |||||||||
21863 |
균주 | 특이적인 활성 [nmol/mg/min] |
ATCC 13032 pClik5MCS | 12.6 |
ATCC 13032 pClik5MCS PmetA metA | 50.7 |
ATCC 13032 pClik5MCS P EF-TU metA | 98.4 |
균주 | 특이적인 활성 [nmol/mg/min] |
ATCC 13032 lysCfbr | 19.3 |
ATCC 13032 Peftu lysCfbr | 49.37 |
균주 | L-라이신 (g/l) |
ATCC13032 | 0 |
ATCC13032 lysCfbr | 10.15 |
ATCC13032 Peftu lysCfbr | 13.2 |
Claims (61)
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- 서열 1로 구성되고, 프로모터 활성을 갖는 핵산 서열, 또는 서열 2로 구성된 핵산 서열을 포함하는 발현 유니트를 포함하며, 상기 프로모터 활성을 갖는 핵산이 유전자의 전사속도를 증가시키거나, 상기 발현 유니트가 유전자의 발현속도를 증가시킴으로써,야생형에 비해 아스파르테이트 키나제의 활성이 증가된 것을 특징으로 하는 유전학적으로 변형된 코리네박테리움 글루타미쿰.
- 제55항에 있어서, 상기 코리네박테리움 글루타미쿰은 호모세린 O-아세틸트랜스퍼라제의 활성이 추가적으로 증가된 것을 특징으로 하는 유전학적으로 변형된 코리네박테리움 글루타미쿰.
- 제55항에 있어서, 상기 코리네박테리움 글루타미쿰은 호모세린 O-아세틸트랜스퍼라제의 활성이 추가적으로 감소된 것을 특징으로 하는 유전학적으로 변형된 코리네박테리움 글루타미쿰.
- 서열 1로 구성되고, 프로모터 활성을 갖는 핵산 서열, 또는 서열 2로 구성된 핵산 서열을 포함하는 발현 유니트를 포함하며, 상기 프로모터 활성을 갖는 핵산이 유전자의 전사속도를 증가시키거나, 상기 발현 유니트가 유전자의 발현속도를 증가시킴으로써, 야생형에 비해 아스파르테이트 키나제의 활성이 증가된, 유전학적으로 변형된 코리네박테리움 글루타미쿰을 배양하는 단계를 포함하는 라이신의 제조방법.
- 제58항에 있어서, 상기 코리네박테리움 글루타미쿰은 호모세린 O-아세틸트랜스퍼라제의 활성이 추가적으로 감소된 것을 특징으로 하는 라이신의 제조방법.
- 서열 1로 구성되고, 프로모터 활성을 갖는 핵산 서열, 또는 서열 2로 구성된 핵산 서열을 포함하는 발현 유니트를 포함하며, 상기 프로모터 활성을 갖는 핵산이 유전자의 전사속도를 증가시키거나, 상기 발현 유니트가 유전자의 발현속도를 증가시킴으로써, 야생형에 비해 아스파르테이트 키나제의 활성이 증가된, 유전학적으로 변형된 코리네박테리움 글루타미쿰을 배양하는 단계를 포함하는 메티오닌의 제조방법.
- 제60항에 있어서, 상기 코리네박테리움 글루타미쿰은 호모세린 O-아세틸트랜스퍼라제의 활성이 추가적으로 증가된 것을 특징으로 하는 메티오닌의 제조방법.
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Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10359594A1 (de) | 2003-12-18 | 2005-07-28 | Basf Ag | PEF-TU-Expressionseinheiten |
DE102004035065A1 (de) * | 2004-07-20 | 2006-02-16 | Basf Ag | P-ET-TS-Expressionseinheiten |
KR100620092B1 (ko) * | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
DE102004061846A1 (de) | 2004-12-22 | 2006-07-13 | Basf Ag | Mehrfachpromotoren |
PL2082045T3 (pl) * | 2006-10-24 | 2015-07-31 | Basf Se | Sposób zmniejszania ekspresji genów poprzez modyfikację częstości występowania kodonów |
WO2008101857A2 (en) | 2007-02-19 | 2008-08-28 | Evonik Degussa Gmbh | Coryneform bacteria with formate-thf-synthetase and/or glycine cleavage activity |
JP2010130899A (ja) | 2007-03-14 | 2010-06-17 | Ajinomoto Co Inc | L−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法 |
JP5493854B2 (ja) | 2007-04-10 | 2014-05-14 | 味の素株式会社 | 有機酸の製造方法 |
EP2192170B1 (en) | 2007-09-04 | 2017-02-15 | Ajinomoto Co., Inc. | Amino acid-producing microorganism and method of producing amino acid |
CN102159720A (zh) * | 2008-04-30 | 2011-08-17 | 赢创德固赛有限公司 | 使用具有降低的异柠檬酸脱氢酶活性的微生物生产甲硫氨酸的方法 |
US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
US9005957B2 (en) | 2008-10-20 | 2015-04-14 | Cj Cheiljedang Corporation | Corynebacterium genus microorganism having ability to produce N-acetyl glucosamine and method for producing N-acetyl glucosamine or glucosamine using the same |
US20120295317A1 (en) | 2009-12-17 | 2012-11-22 | Basf Se | Processes and Recombinant Microorganisms for the Production of Cadaverine |
US9745608B2 (en) | 2011-02-22 | 2017-08-29 | Basf Se | Processes and recombinant microorganisms for the production of cadaverine |
DE102011118019A1 (de) | 2011-06-28 | 2013-01-03 | Evonik Degussa Gmbh | Varianten des Promotors des für die Glyzerinaldehyd-3-phosphat-Dehydrogenase kodierenden gap-Gens |
AR086790A1 (es) | 2011-06-29 | 2014-01-22 | Metabolic Explorer Sa | Un microorganismo para la produccion de metionina con importacion de glucosa mejorada |
JP6100370B2 (ja) | 2012-06-18 | 2017-03-22 | メタボリック エクスプローラー | メチオニンの発酵生産のための組換え微生物 |
KR20140066553A (ko) | 2012-11-23 | 2014-06-02 | 삼성전자주식회사 | 코리네박테리움 속 균주의 신규 프로모터 |
CN103361345B (zh) * | 2013-06-15 | 2016-05-04 | 福州大学 | 重组调控生物元器件强化次级代谢产物生物合成的方法 |
PL3039153T3 (pl) | 2013-08-30 | 2019-02-28 | Evonik Degussa Gmbh | Mikroorganizm do wytwarzania metioniny o ulepszonej aktywności syntazy metioniny i wypływie metioniny |
CN107109359B (zh) | 2014-09-01 | 2021-06-29 | 赢创运营有限公司 | 用于通过具有改进的甲硫氨酸排出的发酵生产甲硫氨酸的方法和微生物 |
KR101677368B1 (ko) | 2015-04-02 | 2016-11-18 | 씨제이제일제당 (주) | 신규 프로모터 및 이의 용도 |
JP7203496B2 (ja) | 2015-05-19 | 2023-01-13 | ミツビシ ケミカル ユーケー リミテッド | メタクリル酸およびその誘導体の生物学的製造方法 |
CN108026516A (zh) | 2015-08-07 | 2018-05-11 | 赢创德固赛有限公司 | 通过发酵的蛋白硫代羧化物依赖性l-甲硫氨酸生产 |
US10801050B2 (en) | 2015-10-23 | 2020-10-13 | Metabolic Explorer | Microorganism modified for the assimilation of levulinic acid |
BR112018010747A8 (pt) | 2015-11-27 | 2019-02-26 | Evonik Degussa Gmbh | método para a produção de l-metionina |
EP3296404A1 (en) | 2016-09-15 | 2018-03-21 | Evonik Degussa GmbH | Modified microorganism for production of methionine |
GB201619827D0 (en) | 2016-11-23 | 2017-01-04 | Lucite Int Uk Ltd | Process for the production of methyl methacrylate |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
EP3415622A1 (en) | 2017-06-14 | 2018-12-19 | Evonik Degussa GmbH | Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases |
JP7498598B2 (ja) | 2020-05-29 | 2024-06-12 | 花王株式会社 | 新規プロモーター |
GB2621337A (en) | 2022-08-08 | 2024-02-14 | Mitsubishi Chemical Uk Ltd | Process for the biological production of methacrylic acid and derivatives thereof |
Family Cites Families (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6696561B1 (en) | 1909-07-09 | 2004-02-24 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport |
JPS5835197A (ja) | 1981-08-26 | 1983-03-01 | Kyowa Hakko Kogyo Co Ltd | プラスミドpcg2 |
US4601893A (en) | 1984-02-08 | 1986-07-22 | Pfizer Inc. | Laminate device for controlled and prolonged release of substances to an ambient environment and method of use |
GB2165546B (en) | 1984-08-21 | 1989-05-17 | Asahi Chemical Ind | A plasmid containing a gene for tetracycline resistance and dna fragments derived therefrom |
DE4027453A1 (de) | 1990-08-30 | 1992-03-05 | Degussa | Neue plasmide aus corynebacterium glutamicum und davon abgeleitete plasmidvektoren |
DE69529026T2 (de) | 1994-07-19 | 2003-07-17 | Hayashibara Biochem Lab | Trehalose, ihre Herstellung und ihre Verwendung |
DE4440118C1 (de) | 1994-11-11 | 1995-11-09 | Forschungszentrum Juelich Gmbh | Die Genexpression in coryneformen Bakterien regulierende DNA |
JP3711658B2 (ja) * | 1996-10-07 | 2005-11-02 | 味の素株式会社 | コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質 |
US6238896B1 (en) | 1997-02-20 | 2001-05-29 | Mitsubishi Rayon Co., Ltd. | Process for producing malonic acid derivatives |
JPH10229891A (ja) | 1997-02-20 | 1998-09-02 | Mitsubishi Rayon Co Ltd | マロン酸誘導体の製造法 |
DE19931317A1 (de) * | 1999-07-07 | 2001-01-11 | Degussa | L-Lysin produzierende coryneforme Bakterien und Verfahren zur Herstellung von L-Lysin |
SK7702000A3 (en) * | 1998-09-25 | 2000-10-09 | Ajinomoto Kk | Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity |
US7892798B2 (en) | 1999-06-25 | 2011-02-22 | Evonik Degussa Gmbh | Nucleic acid molecules encoding metabolic regulatory proteins from Corynebacterium glutamicum, useful for increasing the production of methionone by a microorganism |
WO2001000804A2 (en) | 1999-06-25 | 2001-01-04 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins |
KR20070087090A (ko) | 1999-06-25 | 2007-08-27 | 바스프 악티엔게젤샤프트 | 탄소 대사 및 에너지 생산과 관련된 단백질을 코딩하는코리네박테리움 글루타미쿰 유전자 |
US6822084B1 (en) | 1999-06-25 | 2004-11-23 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding stress, resistance and tolerance proteins |
US7270984B1 (en) | 1999-06-25 | 2007-09-18 | Basf Aktiengesellschaft | Polynucleotides encoding a 6-phosphogluconolactonase polypeptide from corynebacterium glutamicum |
US20030049804A1 (en) | 1999-06-25 | 2003-03-13 | Markus Pompejus | Corynebacterium glutamicum genes encoding metabolic pathway proteins |
KR100878334B1 (ko) | 1999-06-25 | 2009-01-14 | 백광산업 주식회사 | 대사 경로 단백질을 코딩하는 코리네박테리움 글루타미쿰유전자 |
KR100878333B1 (ko) | 1999-06-25 | 2009-01-14 | 백광산업 주식회사 | 항상성 및 적응과 관련된 단백질을 코딩하는코리네박테리움 글루타미쿰 유전자 |
JP2001069979A (ja) * | 1999-08-31 | 2001-03-21 | Japan Tobacco Inc | 発酵法によるl−グルタミン酸の製造方法 |
CA2937907C (en) * | 1999-09-28 | 2017-08-22 | Geneohm Sciences Canada Inc. | Nucleic acids, methods and kits for the detection of campylobacter |
JP4623825B2 (ja) | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | 新規ポリヌクレオチド |
DE10136986A1 (de) | 2000-09-03 | 2002-03-21 | Degussa | Für Gene cysD, cysN, cysK, cysE und cysH kodierende Nukleotidsequenzen |
DE10046870A1 (de) | 2000-09-20 | 2002-03-28 | Basf Ag | Verfahren zur Veränderung des Genoms von Corynebakterien |
DE10056615A1 (de) | 2000-11-15 | 2002-05-29 | Zahnradfabrik Friedrichshafen | Gleitgelagerte, geschweißte Baueinheit |
AU2002230431A1 (en) | 2000-11-15 | 2002-05-27 | Archer-Daniels-Midland Company | Corynebacterium glutamicum promoters |
EP1345714A1 (en) | 2000-12-22 | 2003-09-24 | Basf Aktiengesellschaft | Genes of corynebacterium |
DE10112992A1 (de) | 2001-03-17 | 2002-09-26 | Degussa | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
ES2335750T3 (es) * | 2001-03-30 | 2010-04-05 | Ajinomoto Co., Inc. | Metodo de secrecion y de produccion de proteinas. |
EP1414970A2 (en) | 2001-08-06 | 2004-05-06 | Degussa AG | Production of l-lysine by genetically modified corynebacterium glutamicum strains |
DE10154180A1 (de) | 2001-11-05 | 2003-05-15 | Basf Ag | gene die für genetische Stabilitäts-, genexpressions-und Faltungsproteine codieren |
US7005031B2 (en) | 2002-01-16 | 2006-02-28 | 3M Innovative Properties Company | Pressure sensitive adhesives having quaternary ammonium functionality, articles, and methods |
US7313177B2 (en) | 2003-05-10 | 2007-12-25 | Hewlett-Packard Development Company, L.P. | Reset scheme for I/O pads in a source synchronous system |
CN1890372A (zh) * | 2003-12-18 | 2007-01-03 | 巴斯福股份公司 | 通过发酵制备精细化学品的方法 |
DE10359660A1 (de) | 2003-12-18 | 2005-07-28 | Basf Ag | Psod-Expressionseinheiten |
DE10359595A1 (de) | 2003-12-18 | 2005-07-28 | Basf Ag | Pgro-Expressionseinheiten |
DE10359594A1 (de) | 2003-12-18 | 2005-07-28 | Basf Ag | PEF-TU-Expressionseinheiten |
-
2003
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-
2004
- 2004-12-15 JP JP2006544325A patent/JP2007514425A/ja not_active Withdrawn
- 2004-12-15 ES ES07123785.3T patent/ES2439946T3/es active Active
- 2004-12-15 TW TW93138985A patent/TW200530398A/zh unknown
- 2004-12-15 US US10/582,918 patent/US7785779B2/en active Active
- 2004-12-15 RU RU2006125501/13A patent/RU2006125501A/ru unknown
- 2004-12-15 AU AU2004298546A patent/AU2004298546A1/en not_active Abandoned
- 2004-12-15 WO PCT/EP2004/014266 patent/WO2005059093A2/de active Application Filing
- 2004-12-15 IN IN2598CH2006 patent/IN2006CH02598A/en unknown
- 2004-12-15 CN CN2008100089537A patent/CN101255419B/zh active Active
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- 2004-12-15 CN CNA2004800379281A patent/CN1894412A/zh active Pending
- 2004-12-15 EP EP04803887A patent/EP1697525A2/de not_active Withdrawn
- 2004-12-15 BR BRPI0417710-0A patent/BRPI0417710A/pt not_active IP Right Cessation
- 2004-12-15 CA CA002548349A patent/CA2548349A1/en not_active Abandoned
- 2004-12-15 KR KR1020067014165A patent/KR101162226B1/ko active IP Right Grant
- 2004-12-15 EP EP07123785.3A patent/EP1908842B1/de active Active
- 2004-12-15 MX MXPA06006131A patent/MXPA06006131A/es not_active Application Discontinuation
- 2004-12-17 AR ARP040104735A patent/AR047150A1/es not_active Application Discontinuation
-
2006
- 2006-06-09 NO NO20062683A patent/NO20062683L/no not_active Application Discontinuation
- 2006-07-17 ZA ZA200605863A patent/ZA200605863B/xx unknown
-
2008
- 2008-01-24 IN IN415CH2008 patent/IN2008CH00415A/en unknown
- 2008-02-27 US US12/038,603 patent/US8044191B2/en active Active
- 2008-04-02 JP JP2008095756A patent/JP5710095B2/ja active Active
Non-Patent Citations (2)
Title |
---|
Journal of Biotechnology, Vol.104, No.1-3, pp.311-323 (2003.09.04) |
NCBI, GenBank accession number AP005275 (2002.08.08)* |
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JP5710095B2 (ja) | 2015-04-30 |
EP1697525A2 (de) | 2006-09-06 |
BRPI0417710A (pt) | 2007-03-20 |
US7785779B2 (en) | 2010-08-31 |
MXPA06006131A (es) | 2006-08-11 |
RU2006125501A (ru) | 2008-01-27 |
CN1894412A (zh) | 2007-01-10 |
AR047150A1 (es) | 2006-01-11 |
PL1908842T3 (pl) | 2014-03-31 |
AU2004298546A1 (en) | 2005-06-30 |
EP1908842B1 (de) | 2013-09-18 |
EP1908842A2 (de) | 2008-04-09 |
IN2008CH00415A (ko) | 2008-09-19 |
CA2548349A1 (en) | 2005-06-30 |
JP2007514425A (ja) | 2007-06-07 |
ES2439946T3 (es) | 2014-01-27 |
CN101255419B (zh) | 2012-06-20 |
WO2005059093A3 (de) | 2006-03-02 |
KR20070000431A (ko) | 2007-01-02 |
TW200530398A (en) | 2005-09-16 |
CN101255419A (zh) | 2008-09-03 |
IN2006CH02598A (ko) | 2007-06-08 |
ZA200605863B (en) | 2008-01-30 |
DE10359594A1 (de) | 2005-07-28 |
US20080166775A1 (en) | 2008-07-10 |
US20080118948A1 (en) | 2008-05-22 |
WO2005059093A2 (de) | 2005-06-30 |
NO20062683L (no) | 2006-09-11 |
US8044191B2 (en) | 2011-10-25 |
EP1908842A3 (de) | 2008-08-27 |
JP2008212155A (ja) | 2008-09-18 |
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