KR100971629B1 - cosmetic composition containing herb extracts - Google Patents

Abstract

The present invention is Polygonum multiflorum Thunberg, Polygonum multiflorum Thunberg or Cynanchum wilfordii Hemsley, Lycium chinense MILL, Poria cocos Wolf, Rehmannia glutinosa , Rehmannia glutinosa ( Aparagus lucidus Lindley), Ophiopogon japonicus , Panax schinseng Nees, mixed with steam and fermented by long-term fermentation in traditional grain liquor containing Saccharomyces cerevisiae and Yeast ( Aspergillus oryzae ) The present invention relates to a cosmetic composition containing an extract, the cosmetic composition of the present invention exhibits excellent effects in moisturizing effect, anti-inflammatory effect, free radical scavenging effect and the like.

Cosmetics, Sewao, Sukjiwang, Fermented, Astronomical, Mcmundong

Description

Cosmetic composition containing plant extracts {cosmetic composition containing herb extracts}

The invention enemy sewage (Polygonum multiflorum Thunberg), back sewage (Polygonum multiflorum Thunberg or Cynanchum wilfordii Hemsley), jigolpi (Lycium chinense MILL), baekbokryeong (Poria cocos Wolf), geonjihwang (Rehmannia glutinosa to indicate healing improvement of skin benefit ), Rehmannia glutinosa , Asparagus lucidus Lindley, Ophiopogon japonicus and Panax schinseng Nees, which are steamed with steam and contain yeast ( Saccharomyces cerevisiae ) and yeast ( Aspergillus oryzae ). Long-term fermentation in grain wine provides cosmetics with excellent effects in moisturizing, anti-inflammatory and free radical scavenging effects.

The skin plays a major role in the general metabolism of water in the body. Therefore, the moisture content of the stratum corneum is important for maintaining the surface of the skin soft and healthy. The moisture content of the stratum corneum is closely related to the flexibility and protection of the stratum corneum, depending on the proportion of water reaching the stratum corneum, the rate of water released by evaporation, and the capacity of the stratum corneum to retain water. In addition, the amount of moisture in the stratum corneum produced by regular keratinization is always maintained at 10-20%. This is due to the sebum film, which is a mixture of sweat and sebum, which protects the skin from the outside and prevents water from evaporating from the stratum corneum. However, as the skin ages, the amount of collagen, mucopolysaccharide, and hyaluronic acid in the matrix, which plays an important role in skin elasticity, decreases and the amount of insoluble collagen increases. This makes it difficult for the dermis to keep the skin hydrated and the skin rough. Furthermore, with aging, the function of sebaceous glands and sweat glands decreases, so the amount of sebum and sweat gradually decreases. This causes the sebaceous membranes to not form properly and loses protection against skin hydration, eventually resulting in dry skin (Esko Alanen, Jouni Nuutinen, Kirsi Nickle ㅄ n, Tapani Lahtinen and Jukka Mo ㄸ nkko ㄸ nen, Measurement of hydration). in the stratum corneum with the MoistureMeter and comparison with the Corneometer, Skin Research and Technology , 10: pp 32-37, (2004))

The most common irritant of skin is ultraviolet light. The skin is exposed to ultraviolet light and photochemical reactions continue to occur. The main cause of the recent damage of human skin cells by ultraviolet rays (UV-B and UV-A) is the singlet oxygen ( ¹ O ₂ ), which is the most reactive among the various reactive oxygen species, which is produced by the skin when exposed to sunlight It has been reported that it can cause dermatitis and skin cancer through denaturation and peroxidation of lipids. Failure to absorb or defend against UV rays can result in significant damage to skin cells and tissues. As a result, cellular components such as proteins, lipids, and DNA are damaged by free radicals, and cell functions are altered to promote skin aging and skin cancer. Yaar M., and Gilchrest, BA (1998) Aging versus photoaging: postulated mechanisms and effectors. J. Investig. Dermatol. Symp. Proc. 3: 47-51.

Inflammation is a series of defenses aimed at minimizing the response and restoring damaged areas when cells or tissues are damaged by any cause.Inflammation is the cause of inflammation, including physical factors and acids (trauma, frostbite, radiation). Chemical factors such as acid) and immunological factors caused by antibody reactions are also caused by blood vessels and hormonal imbalances. Anti-inflammatory drugs are called anti-inflammatory agents that work to eliminate inflammation, reduce biologic reactions and symptoms to eliminate inflammation. Materials used for anti-inflammatory purposes so far include nonsteroidal flufenamic acid, ibuprofen, benzydamine, and indomethacin, and prednisolone as a steroid system. , Dexamethasone (dexamethasone) (Kim Chang-jong, Pathophysiology, Hallym Co., p61-69, 1988), allantoin, azulene, ε-aminokiproic acid, hydrocortisone, licoriceic acid and its derivatives (β-glychile) Hydrophilic acid, glycylinic acid derivatives, etc. are known to be effective in anti-inflammatory (Ministry of Gwang-jeong, Ministry of New Cosmetics, Namsan Dang, p162, 1993)

The skin is damaged by these various causes, and various studies have recently been conducted.

Sewage is a perennial plant that grows on vines. The roots form a thick mass. The leaves are heart shaped and stick together. White small flowers bloom in autumn. The plant was originally called Gyo-eung, and it was said that a man named Shaoo ate this herb and ate it. Its main components include oxymethylanthraquinone derivatives (crisopanol and chrysopanolanthrone, emodine, emodine monomethyl ester, piscione and its glycosides, lanes), starch, oil and lecithin in the roots. In motion therapy, roots are used for postpartum and senile constipation, scabies and eczema. When the law is bloody, dizzy, full of heart, and can't sleep, Sukjihwang, donkey, pine root, locust seed, painful waist, oil well, daegu, iron knee root, gojijasaengsam hazelnuts and so on. As such, it has been used for a long time as tonic medicine, gangjeong medicine, and long life medicine.

The phalanx is a bush of 4m long stem. Leaves are willow-shaped or egg-shaped, sticking alternately. A variety of flowers bloom on the leaves and oval fruits ripen reddish-yellow. The fruit of the Gojija tree is called Gojija and its root bark is called Jigolpi. Main ingredients include Betaine, β-sitosterol, Zeaxanthin, Physalien, Meliscic acid, Rutin, Kukoamine A, Steroid Saponin, Nicotianamine, Sugiol, Diosgenin, Total Steroid Phenolic Compound, Vitamin A, B ₁ , B _2, C, linoleic acid, Contains inorganic ingredients. Gojija is described in the medicinal herb, "If you take a long time to strengthen the muscles (筋骨), lighten the body and do not grow old".上 藥 is a valuable medicine that can prolong the life of a person and do not have any side effects and 없으므로 because they can eat a lot or continue to take 害 and 不 老 長壽. Wolfberry is one of these precious medicines. The other names of goji are 明 眼 草 子, 靑 精子, 地 仙子, 地 節 子 and there are many others. As its name suggests, its efficacy is known as a medicinal herb for brightening eyes, maintaining youthfulness and longevity.

Baekbongryeong grows and cultivates where pine trees are cut all over the country. It grows large like a hump from the roots of pine trees that have been cut off for many years, and is found by stabbing a pine tree stump around it. It is a mass about 30 ~ 50㎝ in diameter, rough on the outside like pine bark, white or pink inside. The white inside is called Baekbok-ryeong, and the pink one is called Red Bok-Ryeong. And what surrounds the pine root is called blessed. Its main component contains β-Pachyman and accounts for about 93% of its dry weight. Triterpenoid compounds contain Pachymic acid, Tumulosic acid, 3-β-Hydroxylanosta-7, 9 (11), and 24-trien-21-oic acid. In addition, it contains Chitin, Protein, Fat, Sterol, Lecithin, Glucose, Adenin, Histidine, Choline, β-Pachyman Degrading Enzyme, Lipase and Protease. It is excellent for cancer suppression, stomach, tonic, pancreas and kidney. It has been used as a tonic since ancient times. Pharmacological results show that diuretic, lowering blood sugar, and sedative action.

It is a perennial herb that is 15-25 cm high, and its leaves are long oval or long oval, with pink flowers with multicolored upper part of stem in early summer. Its medicinal plants use the root as a medicinal herb, raw raw sulfur, dried dried guernsey, washed raw roots in water and steamed several times with dried saengjihwang. At the roots are the iridoid glycosides catalpol and mannitol, lechmanine. There are also alkaloids, flavonoids and carotenoids, and the non-saponified parts of the oil include β-sitosterol, stigmasterol and yanthosterol. Clear the blood with hemostasis, diuretics, and releasing the blood in the tissue is a good drug when the joints sprained or bruised bruises when squeezed and attached to loosen the blood and have an analgesic effect. Dried <Geonjihwang> is used as a blood-blooding agent that helps the kidneys and liver function and clears blood. <Sookjihwang> is a medicine that is effective for people who are fragile with blood and tonic. It is a medicine that forms a double wall with ginseng, the covenant of nourishment.

Astronomical dong grows by the sea. Root stock is short and has many cylindrical roots with pointed ends. The base of the stem has egg-shaped pieces of scale. Stems are green, 1 ~ 2m long, vine-shaped, leaf-like branches gathered one to three, running and bowing. The name Cheonmun-dong (天 門冬) means winter herb that opens the door to heaven. The main components of the tuber are asparagine, β-sitosterol, 5-methoxy-asparagine, methylfurfural, mucus and steroid saponin. Dongbogam said that if you take astronomical dong for a long time, your body becomes lighter and you don't starve. It also protects your lungs, so you won't breathe or have a dry cough and have a greater effect on chronic bronchitis in the winter. In addition, diuretic, as well as facilitating the communication of urine, and also tonic effect, and makes the skin smooth.

Macmundong is a perennial green perennial herb, leaves are 2 ~ 4mm wide, gathered. Light branched small flowers bloom. The roots are everywhere. The roots of the tuber are called "Mangmun-dong" and the core is removed and dried. The roots of this plant are similar to barley (mac) roots, and the leaves are not withered even in winter (dong). About 71% of the roots are monosaccharides (about 10%) and low molecular weight polysaccharides (mucus 43%). It is mainly composed of glucose, fructose and sucrose. In addition, there are glycoside opiopogo A, B, C, and D having β-sitosterol and ruscogenin as aglucon. McMoon-Dong and Astronomical-Dong are the same in medicine and have similar effects. Macmundong is a fever-lowering medicine, inflammatory medicine, and coughing sputum sputum, which is usually used when the body is weak, such as sweating and dry mouth due to fever, coughing due to lung fever, dry cough due to lung swelling, continuous mild fever, and stiffness. It is tonic and has a strong and urinating effect.

Ginseng is a perennial herb that is about 60cm high, and the leaf is a double leaf with 5 oval or oval-shaped leaves. From 4 years old, there are 5 ~ 6 leafs. In summer, small flowers gather in an umbrella shape. Mountain ginseng (Yamsam), planted in the field, cultivated ginseng (wonsam), and planted in the mountains are called janggesam. Cultivated ginseng is cut and dried by white ginseng and steamed red ginseng. Roots include glycosides, essential oils, oils, amino acids, alkaloids, carbohydrates, vitamins, resins, and trace elements. Glycosides in ginseng have been studied for a long time. Glycosides previously known to be isolated from ginseng, panakuilon, panaksik, panaxol, and ginsenin are mixtures of glycosides or panaxadiol-2-glucoside, and panacicon, α-panaxin, etc. It is recognized as 3-glucoside. In oriental medicine, it is used for bullying as a bully, tonic, tonic or resurrection neuro stabilizer, and it is used for the sick who are inferior in metabolic function of kidney due to active metabolic function of heart and diuretic effect. Rejuvenation, mental stability, treatment of chronic gastritis and peptic ulcer, liver function control, forgetfulness, chronic bronchitis, bronchial asthma. And lowering blood sugar, anti-cancer, anti-reflective, anti-allergic reactions and inhibit aging.

The present invention will be described in more detail as follows. Drip Sewage, Baekhasuo, Jigolpi, Baekbokyeong, Guernsey, Sukjiwang, Astronomical, Macmundong, Ginseng are mixed and steamed at 80 ~ 120 ℃ with steam and hot-air dried at 80 ~ 120 ℃. After repeating this process 3 to 10 times, fermentation of fermentation broth such as rice, millet, glutinous rice, brown rice, barley using Saccharomyces cerevisiae and yeast ( Aspergillus oryzae ) is added to fermentation for a certain period (10-30 days). . After fermentation is completed, the injected medicinal herbs are filtered to remove the filtrate and dried by hot air at 80 ~ 120 ℃. To the dried medicament is added 1-20 times by volume of one or more solvents selected from the group consisting of water, ethanol, glycerin, butylene glycol and propylene glycol as extraction solvents. As the extraction method, a cooling condenser is installed and extracted by heating at 50-95 ° C. for 4-20 hours while preventing the solvent from evaporating, or by extracting the active ingredient at 5 to 37 ° C. for 1 to 15 days. Thus extracted extract is mixed in the range of 0.01 ~ 30% by weight (0.0001 ~ 0.30% by weight as dry weight) and added to the cosmetic to provide a cosmetic composition having excellent effects in moisturizing effect, anti-inflammatory effect, free radical scavenging effect.

Accordingly, an object of the present invention is to provide a substance having excellent efficacy such as excellent moisturizing effect, anti-inflammatory effect, free radical scavenging effect, etc. and having no problem in stability and safety from natural products.

Another object of the present invention is to provide a cosmetic composition comprising an extract having such efficacy.

The present inventors studied in various ways to find the fundamental healing material for the cause of skin damage, and after steaming with water vapor by mixing red sewage, white sewage, phalanges, baekbokyeong, gunjihwang, sukjihwang, astronomical dong, macmundong, ginseng The fermentation extract prepared by drying and aging in traditional grain liquor showed very good moisturizing effect, anti-inflammatory effect and free radical scavenging effect.

The preparation method of the mixed extract of Red Sewage, White Sewage, Jigolpi, Paekbokyeong, Gunjihwang, Sukjihwang, Astronomical Dong, Macmundong, Ginseng of the present invention is as follows.

Take a certain amount of Drip Sewage, Baek Hasuo, Jigolpi, Paekbokyeong, Guernsey, Sukjiwang, Astronomical, Macmundong, Ginseng, steam with 80 ~ 120 ℃ and steam dry at 80 ~ 120 ℃. After repeating this process 3 to 10 times, the above-mentioned dry medicine is added to fermentation broths such as rice, millet, glutinous rice, brown rice, and barley using Saccharomyces cerevisiae and Yeast bacteria ( Aspergillus oryzae ). 30 days) fermentation. After fermentation is completed, the added medicines are filtered to remove the filtrate and dried by hot air again at 80 ~ 120 ℃. The dried drug is added 1-20 times by volume of one or more solvents selected from the group consisting of water, ethanol, glycerin, butylene glycol and propylene glycol as extraction solvents. Extraction method is a method of extracting by heating at 50-95 ℃ for 4-20 hours in the state of preventing the evaporation of the solvent is equipped with a cooling condenser, or by extracting the active ingredient by deposition for 1-15 days at 5 ~ 37 ℃. Can be used. Thus extracted extract is mixed in a range of 0.01 to 30% by weight (0.0001 to 0.30% by weight as dry weight) is added to the cosmetic.

Fermented extracts of Red Sewage, White Sewage, Jigolpi, Paekbokyeong, Guernsey, Sukjiwang, Astronomical, Macmundong, and Ginseng can be added to the cosmetics in an amount of 0.01% to 30% by weight of the liquid, and a distillation unit equipped with a cooling condenser The mixture was concentrated under reduced pressure while recovering the solvent evaporated using the same, and then added to the cosmetic in an amount of 0.0001 to 0.30% as the dry weight, preferably 0.001 to 0.1% as the dry weight.

The following Examples and Experimental Examples illustrate the content of the present invention, but the content of the present invention is not limited thereto.

The present invention is Polygonum multiflorum Thunberg, Polygonum multiflorum Thunberg or Cynanchum wilfordii Hemsley, Lycium chinense MILL, Poria cocos Wolf, Rehmannia glutinosa , Rehmannia glutinosa ( Aparagus lucidus Lindley), Ophiopogon japonicus , Panax schinseng Nees, mixed with steam and fermented by long-term fermentation in traditional grain liquor containing Saccharomyces cerevisiae and Yeast ( Aspergillus oryzae ) The present invention relates to a cosmetic composition containing an extract, the cosmetic composition of the present invention exhibits excellent effects in moisturizing effect, anti-inflammatory effect, free radical scavenging effect and the like.

Example  1. Capital increase

Baek Ha Shou 160g, Jeok Hao 160g, Jigolpi 200g, Baekbokyeong 200g, Geonjihwang 120g, Sukjihwang 120g, Macmundong 120g, Cheonmundong 120g, Ginseng 120g, mixed in a container containing 2kg of purified water and steamed for 3 hours at 100 ℃ 1630 g was obtained.

Example  2. Dry

The medicine prepared in Example 1 was dried at 80 ° C. for 3 hours to obtain 1230 g.

Example  3. Treatment of pharmaceutical

The same amount as in Example 1 Baek Ha Shou 160g, Red Sewage 160g, Jigolpi 200g, Baekbokyeong 200g, Dried Guhwang 120g, Sukjihwang 120g, Mcmundong 120g, Cheonmundong 120g, ginseng 120g (total 1320g) as shown in Table 1 1 and Example 2 were repeated to prepare Examples 3-1 to 3-5.

Capital increase dry Weight after finish Example 3-1 0 times 0 times 1320 g Example 3-2 1 time 1 time 1230 g Example 3-3 3rd time 3rd time 1195 g Example 3-4 5 times 5 times 1100 g Example 3-5 9th 9th 1085 g

Example  4. Preparation of Fermentation Broth

Example 4-1 Preparation of Nonfermented Rice Fermented Broth

Wash 1kg of non-glutinous rice, clean it, soak it in 2L of water, dry it, grind it into flour, add 8g of water, boil nonglutinous rice, knead it, cool it, mix it with 1kg of yeast powder, and ferment it in a jar for 3 days to make base liquor. Fermented liquor was prepared by putting fermented rice in 2kg of steamed non-glutinous rice and fermenting for 21 days.

Example 4-2 Preparation of Glutinous Rice Fermentation Broth

Wash 1kg of glutinous rice flour, add 0.5L of water, knead it, steam it with a hole cake, cool it, mix it with 1kg of yeast powder, and ferment it in a jar for 3 days to make base liquor. Fermented liquor was prepared by fermenting 14 ml of steamed glutinous rice for 4 days.

Example  5. Raw material fermentation in fermentation broth

The medicines (1085g ~ 1320g) prepared by the method of Examples 3-1 to 3-5 were put into 4kg of the fermentation broths of Examples 4-1 and 4-2 as shown in Table 2 below and fermented for 7 days, respectively. To 5-10 was prepared.

Example Medicine
(Recovery amount according to each embodiment) Fermentation broth 4 kg Yield (dry weight) Example 5-1 Example 3-1 (1320g) Example 4-1 1190 Example 5-2 Example 3-2 (1230 g) Example 4-1 1059 Example 5-3 Example 3-3 (1195 g) Example 4-1 1012 Example 5-4 Example 3-4 (1100 g) Example 4-1 986 Example 5-5 Example 3-5 (1085 g) Example 4-1 913 Example 5-6 Example 3-1 (1320g) Example 4-2 1140 Example 5-7 Example 3-2 (1230 g) Example 4-2 1007 Example 5-8 Example 3-3 (1195 g) Example 4-2 1004 Example 5-9 Example 3-4 (1100 g) Example 4-2 960 Example 5-10 Example 3-5 (1085 g) Example 4-2 932

Example  6. Preparation of Extract

Example 6-1 Preparation of Control

Baek Ha Sewong 160g, Jeok Ha Sew 160g, Jigolpi 200g, Baekbokryeong 200g, Geonjihwang 120g, Sukjihwang 120g, Macmundong 120g, Cheonmundong 120g, Ginseng 120g, mixed in 30% (w / w) ethanol 4kg and aged at 25 ℃ for 7 days do. After aging, the resultant was filtered with 0.45 um filter paper to obtain 2.84 kg (solid content 1.15%) of the extract.

Examples 6-2 to 6-11 Preparation of Experiment Group

The obtained products of Examples 5-1 to 5-10 of Table 2 were dried to mix each mixed raw material in 4 kg of 30% (w / w) hydrous ethanol and aged at 25 ° C. for 7 days. After aging, the resultant was filtered with a filter paper of 0.45 um to obtain each extract as shown in Table 3 below.

Raw material (dry weight) Extract (solid) Example 6-2 Example 5-1 (1190 g) 2.65 kg (1.05%) Example 6-3 Example 5-2 (1059 g) 2.70 kg (1.25%) Example 6-4 Example 5-3 (1012 g) 2.45 kg (1.20%) Example 6-5 Example 5-4 (986 g) 2.35 kg (1.20%) Example 6-6 Example 5-5 (913 g) 2.45 kg (1.05%) Example 6-7 Example 5-6 (1140 g) 2.70 kg (1.35%) Example 6-8 Example 5-7 (1007 g) 2.70 kg (1.30%) Example 6-9 Example 5-8 (1004 g) 2.55 kg (1.05%) Example 6-10 Example 5-9 (960 g) 2.40 kg (1.35%) Example 6-11 Example 5-10 (932 g) 2.55 kg (1.35%)

Example  7. Preparation by Extraction Solvent

1 kg of the raw material prepared by the process of Example 6-6 was prepared using the solvent of Table 4 to prepare the extract of Examples 7-1 to 7-3.

Extraction solvent Recovery amount (solid content) Example 7-1 30% water butylene glycol 2.75kg (1.45%) Example 7-2 Purified water 2.36kg (1.00%) Example 7-3 95% water ethanol 2.45kg (1.40%)

Experimental Example  One. Free radical  Efficacy Experiment

The free radical scavenging effect of the mixed fermentation extracts of the Dried Sewage, Baekshou, Jigolpi, Paekbokyeong, Guernsey, Sukjihwang, Cheonmun-dong, Macmun-dong, and Ginseng were measured, and the IC ₅₀ values of each Example were determined based on the solid content in Table 5. As shown.

Experiment method

The complex extracts were reacted with 2 mL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution at room temperature for 10 minutes at different concentrations, and then absorbance was measured at 520 nm (SC ₅₀ : free radical scavenging). The concentration of the extract that increases the activity by 50%). At this time, the control test is performed simultaneously.

 Effect of Free Radical Scavenging Effect on Steamed Fermented Extracts Red sesame seed, Baek se shou, Jigolpi, Baekbokryeong, Geonjihwang, Sukjihwang, Cheonmundong, Mcmundong, Ginseng complex fermentation extract SC ₅₀ (ug / ml) Example 6-1 More than 500 Example 6-2 232 Example 6-3 240 Example 6-4 289 Example 6-5 214 Example 6-6 285 Example 6-7 238 Example 6-8 215 Example 6-9 350 Example 6-10 225 Example 6-11 367 Example 7-1 176 Example 7-2 198 Example 7-3 203

Experimental Example  2. Anti-inflammatory test

The anti-inflammatory effect of the fermented extract of red sesame seed, Baek se shou, Jigolpi, baekbokyeong, geonjihwang, sukjihwang, cheonmundong, mackmundong, ginseng was measured.

Experiment method

Using the left ear of the mouse as the control site and the right ear as the test site, the ear was washed with ethanol before applying the sample, and 20 μl of the sample was continuously applied once a day for 4 days and ethanol was applied to the left ear 1 hour after the last application. , Arachidonic acid (Arachidonic acid) was applied to the right ear, and after 1 hour, the degree of ear edema (ear edema) was repeatedly measured on both ears three times with a micrometer.

The anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group and the results are shown in Table 6.

※ Inhibition Rate (%) = (A-B) / A × 100

A: Average thickness of control ear (thickness of arachidonic acid treated ear (right ear)-thickness of untreated ear (left ear))

B: thickness of ear of fermented extract applicator group (thickness of arachidonic acid treated ear (right ear)-thickness of untreated group ear (left ear)

 Anti-inflammatory Effect of Steam Fermented Extract sample Contained in the reaction solution
Final solids concentration (mg / ear) Ear thickness (㎛) % Inhibition Before sample processing After sample processing Arachidonic acid 2.0 243 498 - Indomethacin 1.0 244 442 22.4 Example 6-1 1.0 254 503 2.5 Example 6-2 1.0 265 480 15.8 Example 6-3 1.0 234 446 16.7 Example 6-4 1.0 265 471 19.4 Example 6-5 1.0 255 456 21.3 Example 6-6 1.0 255 453 22.5 Example 6-7 1.0 256 455 21.9 Example 6-8 1.0 254 452 22.5 Example 6-9 1.0 253 450 22.6 Example 6-10 1.0 259 459 21.5 Example 6-11 1.0 240 435 23.6 Example 7-1 1.0 242 409 34.5 Example 7-2 1.0 242 414 32.5 Example 7-3 1.0 235 424 25.9

Experimental Example  3. Moisturizing Experiment

The moisturizing effect was measured using Corneometer CM 825 by Courage + Khazaka for the complex increase fermentation extracts of Dr. Sewage, Baekshou, Jigolpi, Paekbokyeong, Geonjihwang, Sukjihwang, Astronomical Dong, Macmundong, and Ginseng, Example 6-1. For ˜6-11 and 7-1 to 7-3, the solid content was fixed to 1% by weight and diluted with an extraction solvent to conduct a moisturizing experiment. The values after 40 minutes of sample application were measured and shown in Table 7.

Experiment method

Twenty women in their 20s were washed several times with water in the upper arm 2 × 2 ㎠ and 8 sections, and water was removed without irritation. After 5 minutes, apply 10 µl of the sample to each of the 2 sections. After 10 minutes, measure 5 times per compartment using the Corneometer. Measure 6 times at 5 minute intervals for one compartment. The room temperature was 20 ° C and relative humidity 20%.

Water contents (%) = Corneometer Value after sample application-Corneometer Value before sample application, and data value was expressed as the average value for 20 people.

 Moisturizing Effect of Steamed Fermented Extract sample Water contents after 40 minutes (%) Example 6-1 (Control) 3.4 Example 6-2 8.5 Example 6-3 9.5 Example 6-4 11.5 Example 6-5 10.3 Example 6-6 12.3 Example 6-7 13.4 Example 6-8 12.5 Example 6-9 12.0 Example 6-10 13.6 Example 6-11 9.8 Example 7-1 16.5 Example 7-2 12.7 Example 7-3 15.4

Prescription Example  Pharmacy

The following formulation showed an overall high effect of the extract extracted with 30% butylene glycol of Example 7-1 based on the results measured in Experimental Examples 1 to 3, and accordingly the extract of Example 7-1 Prepared using.

Prescription Example  1. (skin lotion)

Prescription examples of the lotion (skin lotion) of the cosmetics containing the complex increase fermentation extracts of the drop, Sewage, Baekshou, Jigolpi, Baekbokyeong, Guernsey, Sukjiwang, Astronomical, Macmundong, Ginseng are as follows. The sample implemented here refers to the thing of Example 7-1.

                                                               Unit: weight% Number Raw material Formulation Example 1 Comparative Formulation Example 1 One  The Red Sewage Sewage, White Sewage, Jigolpi, Baekbokyeong, Guernsey Hwang, Sukji Hwang, Cheonmun-dong, Mcmun-dong, Ginseng Complex Increased Fermentation Extract 5.0 - 2 glycerin 3.0 3.0 3 Butylene Glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene (60) Cured Castor Oil 1.0 1.0 6 ethanol 10.0 10.0 7 Triethanolamine 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Pigment a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Balance Balance

<Production method>

Formulation Example 1 2, 3, 4, and 8 in 11 are added in order to dissolve, and then dissolved. 5 is heated to 60 ° C. for dissolution and 10 times is added to 11 for input stirring.

Finally, 1, 6, 7, and 9 were added to the mixture, sufficiently stirred, and aged.

Comparative Formulation Example 1: No. 2, 3, 4, and 8 were added to No. 11 in order, followed by stirring to dissolve. Then, No. 5 was heated to about 60 ° C. to dissolve.

Finally, 6, 7, and 9 are added and sufficiently stirred before aging.

Prescription Example  2. (Nutrition Lotion)

Prescription examples of nutritional lotion in the cosmetics containing the complex increase fermentation extract of the drop, Sewage, Baekhasuo, Jigolpi, Baekbokyeong, Guernsey, Sukjiwang, Astronomical, Macmundong, Ginseng are as follows.

The sample implemented here refers to the thing of Example 7-1.

                                                                Unit: weight% Number Raw material Formulation Example 2 Comparative Formulation Example 2 One   The Red Sewage Sewage, White Sewage, Jigolpi, Baekbokyeong, Guernsey Hwang, Sukji Hwang, Cheonmun-dong, Mcmun-dong, Ginseng Complex Increased Fermentation Extract 10.0 - 2 Beeswax 1.0 1.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.5 0.5 5 Liquid paraffin 10.0 10.0 6 Sorbitan stearate 1.0 1.0 7 Lipophilic Monostearic Acid Glycerin 0.5 0.5 8 Stearic acid 1.5 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 1.0 10 Propylene glycol 3.0 3.0 11 Carboxy Vinyl Polymer 0.1 0.1 12 Triethanolamine 0.2 0.2 13 antiseptic a very small amount a very small amount 14 Pigment a very small amount a very small amount 15 Spices a very small amount a very small amount 16 Purified water Balance Balance

<Production method>

Formulation Example 2: After mixing 10, 11, 13, 16 and heating to 80 ~ 85 ℃ to put into the manufacturing unit and then actuated emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 12 It is heated to 80 ~ 85 ℃ and then emulsified. After emulsification, cool down to 50 ℃ with stirring using a stirrer, add 15 times, cool down to 45 ℃, add 14 times, add 1 to 35 ℃, and cool to 25 ℃ before ripening.

Comparative Formulation Example 2: After mixing and stirring 10, 11, 13, and 16 to 80-85 ° C. and putting them in the manufacturing part, the emulsifier was activated and 2, 3, 4, 5, 6, 7, 8, 9, 12 After heating to 80 ~ 85 ℃ to emulsify. After emulsification, cool down to 50 ℃ with stirring using a stirrer, add 15 times, cool down to 45 ℃, add 14 times, cool to 25 ℃, and mature.

Prescription Example  3 (nutrition cream)

Prescription examples of nutritional cream in cosmetics containing a complex increase fermentation extract of the red sessau, Baek Ha Shou, Jigolpi, Baekbokyeong, Guernsey Hwang, Sukjiwang, Astronomical Dong, Mcmundong, Ginseng are as follows.

The sample implemented here refers to the thing of Example 7-1.

                                                              Unit: weight% Number Raw material Formulation Example 3 Comparative Formulation Example 3 One   The Red Sewage Sewage, White Sewage, Jigolpi, Baekbokyeong, Guernsey Hwang, Sukji Hwang, Cheonmun-dong, Mcmun-dong, Ginseng Complex Increased Fermentation Extract 10.0 - 2 Beeswax 8.0 8.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.5 0.5 5 Liquid paraffin 20.0 20.0 6 Sorbitan stearate 1.0 1.0 7 Lipophilic Monostearate Glycerin 0.5 0.5 8 Stearic acid 1.5 1.5 9 Glyceryl Stearate / Pig-400 Stearate 1.0 1.0 10 Propylene glycol 6.0 6.0 11 Triethanolamine 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Pigment a very small amount a very small amount 14 Spices a very small amount a very small amount 15 Purified water Balance Balance

<Production method>

Formulation Example 3: 10, 12, 15 while stirring and mixing, heated to 80 ~ 85 ℃ while mixing into the manufacturing unit and then actuated emulsifier and 2, 3, 4, 5, 6, 7, 8, 9, 11 80 It is emulsified after heating to ˜85 ℃. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add 14 times, cool to 45 ℃, add 13 times, and add 1 once when cooled to 35 ℃. It is cooled to 25 ° C. and aged.

Comparative Formulation Example 3: 10, 12, and 15 were stirred and mixed, heated to between 80 and 85 ° C., introduced into the production unit, and then emulsified and 2, 3, 4, 5, 6, 7, 8, 9, 11 were prepared. It is heated to 80 ~ 85 ℃ and then emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and then, No. 14 was added, cooled to 45 ° C., and then No. 13 was added, cooled to 25 ° C., and aged.

Prescription Example  4.( Massage  cream)

The prescription example of the massage cream in the cosmetic containing the drop shou, Baek shou, Jigolpi, Baekbokyeong, Guernsey, Sukjiwang, Tianmen-dong, Mcmun-dong, ginseng complex increase fermentation extract is as follows.

The sample implemented here refers to the thing of Example 7-1.

                                                                Unit: weight% Number Raw material Formulation Example 4 Comparative Formulation Example 4 One  The Red Sewage Sewage, White Sewage, Jigolpi, Baekbokyeong, Guernsey Hwang, Sukji Hwang, Cheonmun-dong, Mcmun-dong, Ginseng Complex Increased Fermentation Extract 10.0 - 2 Beeswax 10.0 10.0 3 Polysorbate 60 1.5 1.5 4 Sorbitan sesquioleate 0.8 0.8 5 Liquid paraffin 40.0 40.0 6 Squalane 5.0 5.0 7 Lipophilic Monostearic Acid Glycerin 1.0 1.0 8 Carboxy Vinyl Polymer 0.2 0.2 9 Butylene glycol 6.0 6.0 10 glycerin 6.0 6.0 11 Triethanolamine 0.2 0.2 12 antiseptic a very small amount a very small amount 13 Pigment a very small amount a very small amount 14 Spices a very small amount a very small amount 15 Purified water Balance Balance

<Production method>

Formulation Example 4 8, 9, 10, 12, 16 while mixing and stirring, heated to 80 ~ 85 ℃ while stirring into the manufacturing unit, and then actuated emulsifier and 2, 3, 4, 5, 6, 7, 11 80 It is emulsified after heating to ˜85 ℃. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add 14 times, cool to 45 ℃, add 13 times, and add 1 once when cooled to 35 ℃. It is cooled to 25 ° C. and aged.

Comparative Formulation Example 4 8, 9, 10, 12, 16 was stirred and mixed, heated to 80 ~ 85 ℃ to put into the manufacturing unit, then the emulsifier was activated and 2, 3, 4, 5, 6, 7, 11 It is heated to 80 ~ 85 ℃ and then emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring using a stirrer, and then, No. 14 was added, cooled to 45 ° C., and then No. 13 was added, cooled to 25 ° C., and aged.

Test Example  1. Experiment to confirm skin improvement effect

Formulation Example 3 and Comparative Formulation Example 3 When the skin was applied to the nourishing cream, the following clinical confirmation experiment was carried out to confirm the skin damage improvement effect. To the same person, the nutritional cream of Formulation Example 3 was applied to the left hand and the nutritional cream of Comparative Formulation Example 3 to the right hand every evening, and then applied to the skin once a day for 60 days. Was measured.

 Skin Enhancement Effect of Steamed Fermented Extract Sample Survey Item Improve Atopy Condition Remarks Number of people %
Formulation Example 3 very good 10 50 good 7 35 is average One 5 so so 2 10
Comparative Formulation Example 3 very good One 5 good 7 35 is average 6 30 so so 6 30

As a result of the investigation, the nutrient cream (Formulation Example 3) prepared by adding the fermented extract of Drainage Sewage, Baekha Sewage, Jigolpi, Baekbokyeong, Geonjihwang, Sukjihwang, Cheonmun-dong, Macmun-dong, and ginseng was not added. It can be seen that the skin improving effect is superior to Comparative Formulation Example 3.

Claims (6)

Fermented in traditional grain wine containing Saccharomyces cerevisiae and Yeast bacteria Aspergillus oryzae Cosmetic composition containing 0.01 to 30% by weight of the fermentation extract extracted by aging. According to claim 1, wherein the weight percent of the drug when mixed is Baek Sewage 5-15%, Drip Sewage 5-15%, Jigolpi 10-20%, Baekbokryeong 10-20%, Dried Guhwang 5-15%, Sukji sulfur 5-15% , Mcmundong 5-15%, cheonmundong 5-15%, ginseng cosmetic composition, characterized in that 5-15%. The cosmetic composition according to claim 1 or 2, wherein the pharmaceutical mixture is prepared by steaming the raw material with steam at 80 to 120 ° C and hot air drying at 80 to 120 ° C. According to claim 1 or 2, traditional grain liquor Saccharomyces rice, millet, glutinous rice, brown rice or barley cerevisiae ) or yeast ( Aspergillus) oryzae ) using a cosmetic composition, characterized in that fermentation for 10 to 30 days. The cosmetic composition according to claim 1 or 2, wherein the extraction solvent is at least one selected from water, ethanol, butylene glycol or propylene glycol. According to claim 5, The extraction method is equipped with a cooling condenser to prevent the extraction solvent is evaporated while extracting by heating for 4-20 hours at 50-95 ℃, or deposited for 5 days at 1 ~ 15 days at 5 ~ 37 ℃ Cosmetic composition, characterized in that the method of extraction.