KR100938415B1 - 밀의 검사 방법 - Google Patents
밀의 검사 방법 Download PDFInfo
- Publication number
- KR100938415B1 KR100938415B1 KR1020047011191A KR20047011191A KR100938415B1 KR 100938415 B1 KR100938415 B1 KR 100938415B1 KR 1020047011191 A KR1020047011191 A KR 1020047011191A KR 20047011191 A KR20047011191 A KR 20047011191A KR 100938415 B1 KR100938415 B1 KR 100938415B1
- Authority
- KR
- South Korea
- Prior art keywords
- wheat
- food
- seq
- primer
- dna
- Prior art date
Links
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cereal-Derived Products (AREA)
Abstract
Description
Claims (11)
- 식품으로부터 DNA를 추출하고, Wtr01(서열번호 1) 및 Wtr10(서열번호 2)의 프라이머를 사용하여 PCR을 실시하고, 프라이머의 증폭산물을 측정하고,강력밀, 중력(준강력)밀, 박력(薄力)밀, 듀럼(마카로니)밀, 기타 식용의 단립계(單粒系)밀로 이루어진 밀군에서 선택되는 1종 이상의 밀이 측정되고, 호밀은 측정되지 않는 식품 중의 밀의 유무를 측정하는 방법.
- 제1항에 있어서, 상기 프라이머의 증폭산물을 전기영동에 의해 측정하고, 밴드를 검출하는 식품 중의 밀의 유무를 측정하는 방법.
- 제1항에 있어서, 식품 중의 미량성분을 식품에 표시하기 위해서, 제1항에 기재된 방법을 사용하여 밀의 유무를 측정하고, 식품에 표시하는 식품 중의 밀의 유무를 측정하는 방법.
- 식품 섭취자에게 유해한 밀의 알레르겐을 함유하는 식품을 식별하기 위해서, 제1항에 기재된 방법을 사용하여, 식품 중의 밀의 유무를 측정하는 방법.
- 제1항에 있어서, 상기 식품이 가공처리된 식품 또는 식품원료인 방법.
- 식품 중의 밀의 유무 또는 농도를 측정하기 위한 제1항에 기재된 PCR용 프라이머.
- 제1항에 기재된 프라이머를 포함하는 식품 중의 밀의 유무 또는 농도를 측정하기 위한 키트.
- 삭제
- 삭제
- 삭제
- 삭제
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002039040 | 2002-02-15 | ||
JPJP-P-2002-00039040 | 2002-02-15 | ||
JP2002132119 | 2002-03-29 | ||
JPJP-P-2002-00132119 | 2002-03-29 | ||
PCT/JP2002/009983 WO2003068989A1 (fr) | 2002-02-15 | 2002-09-26 | Procédé de détection de blé |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20040083428A KR20040083428A (ko) | 2004-10-01 |
KR100938415B1 true KR100938415B1 (ko) | 2010-01-22 |
Family
ID=27736531
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020047011191A KR100938415B1 (ko) | 2002-02-15 | 2002-09-26 | 밀의 검사 방법 |
Country Status (10)
Country | Link |
---|---|
US (1) | US7629118B2 (ko) |
EP (1) | EP1482058B1 (ko) |
JP (1) | JP3903044B2 (ko) |
KR (1) | KR100938415B1 (ko) |
CN (1) | CN1322144C (ko) |
AT (1) | ATE432363T1 (ko) |
AU (1) | AU2002332326B2 (ko) |
CA (1) | CA2476581C (ko) |
DE (1) | DE60232466D1 (ko) |
WO (1) | WO2003068989A1 (ko) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2180051B1 (en) * | 2004-04-09 | 2011-11-16 | Nisshin Seifun Group Inc. | Method for detecting and quantifying endogenous wheat DNA sequence |
KR100720867B1 (ko) * | 2005-05-31 | 2007-05-23 | 주식회사 코젠바이오텍 | 알레르기 유발 식품 검출용 프라이머 및 이를 이용한알레르기 유발 식품 검출방법 |
WO2007132760A1 (ja) * | 2006-05-15 | 2007-11-22 | Nisshin Seifun Group Inc. | コムギ内在性dnaの検出・定量法、および被検試料における遺伝子組換えコムギの混入率の決定方法 |
JP5917788B2 (ja) | 2009-12-21 | 2016-05-18 | 国立研究開発法人農業・食品産業技術総合研究機構 | コムギ内在性遺伝子の検出及び定量方法 |
JP5625211B2 (ja) * | 2009-12-21 | 2014-11-19 | 独立行政法人農業・食品産業技術総合研究機構 | 普通系コムギを定性的及び定量的に検出する方法 |
CN101724707B (zh) * | 2010-01-21 | 2012-08-22 | 曹际娟 | 食品中四种致敏原麸质成分实时荧光pcr检测引物和探针、试剂盒及检测方法 |
JP7420330B2 (ja) * | 2019-06-13 | 2024-01-23 | 学校法人藤田学園 | アレルギーの抗原およびそのエピトープ |
CN114369677A (zh) * | 2022-01-04 | 2022-04-19 | 江汉大学 | 一种检测小麦转基因成分和转基因品系的引物组合、试剂盒、检测方法及应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998004737A1 (en) * | 1996-07-25 | 1998-02-05 | The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Detection of d genome in wheat species |
-
2002
- 2002-09-26 WO PCT/JP2002/009983 patent/WO2003068989A1/ja active Application Filing
- 2002-09-26 JP JP2003568100A patent/JP3903044B2/ja not_active Expired - Lifetime
- 2002-09-26 US US10/504,599 patent/US7629118B2/en not_active Expired - Lifetime
- 2002-09-26 DE DE60232466T patent/DE60232466D1/de not_active Expired - Lifetime
- 2002-09-26 AT AT02768096T patent/ATE432363T1/de not_active IP Right Cessation
- 2002-09-26 EP EP02768096A patent/EP1482058B1/en not_active Expired - Lifetime
- 2002-09-26 CA CA2476581A patent/CA2476581C/en not_active Expired - Lifetime
- 2002-09-26 CN CNB028280741A patent/CN1322144C/zh not_active Expired - Fee Related
- 2002-09-26 KR KR1020047011191A patent/KR100938415B1/ko active IP Right Grant
- 2002-09-26 AU AU2002332326A patent/AU2002332326B2/en not_active Ceased
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998004737A1 (en) * | 1996-07-25 | 1998-02-05 | The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Detection of d genome in wheat species |
Also Published As
Publication number | Publication date |
---|---|
CN1620514A (zh) | 2005-05-25 |
US20050272033A1 (en) | 2005-12-08 |
AU2002332326A1 (en) | 2003-09-04 |
KR20040083428A (ko) | 2004-10-01 |
CN1322144C (zh) | 2007-06-20 |
CA2476581A1 (en) | 2003-08-21 |
WO2003068989A1 (fr) | 2003-08-21 |
JPWO2003068989A1 (ja) | 2005-06-02 |
US7629118B2 (en) | 2009-12-08 |
EP1482058B1 (en) | 2009-05-27 |
JP3903044B2 (ja) | 2007-04-11 |
ATE432363T1 (de) | 2009-06-15 |
EP1482058A4 (en) | 2005-10-26 |
AU2002332326B2 (en) | 2008-01-31 |
CA2476581C (en) | 2012-09-04 |
DE60232466D1 (de) | 2009-07-09 |
EP1482058A1 (en) | 2004-12-01 |
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