KR100895091B1 - MULBERY COMPOSITION AND MULBERY COMPOSITION ERMENTED BY STARTER OF Lactobacillus sp. - Google Patents

Abstract

The present invention relates to a mixed mulberry leaf powder composition and fermentation composition, and more specifically, to improve the digestive function and constipation improvement effect by fermenting the mixed mulberry leaf powder composition and mulberry leaf powder mixed with green tea powder and chlorella powder in mulberry leaf powder with starter It relates to an improved mulberry leaf powder fermentation composition.

The mixed mulberry leaf powder composition according to the present invention is characterized in that 5% by weight of green tea powder and 5% by weight of chlorella powder to mulberry leaf powder 90% by weight, the mulberry leaf powder fermentation composition is fermented by inoculating lactic acid bacteria starter to the mulberry leaf powder It is characterized by.

Mulberry leaves, fermented

Description

MULBERY COMPOSITION AND MULBERY COMPOSITION ERMENTED BY STARTER OF Lactobacillus sp.

The present invention relates to a mixed mulberry leaf powder composition and fermentation composition, and more specifically, to improve the digestive function and constipation improvement effect by fermenting the mixed mulberry leaf powder composition and mulberry leaf powder mixed with green tea powder and chlorella powder in mulberry leaf powder with starter It relates to an improved mulberry leaf powder fermentation composition.

Constipation has long been known as the source of all kinds of illnesses, and the appetite is not always present and the abdomen is bloated, and the excreted toxins from unexcreted stools are absorbed into the intestine, which is absorbed into the blood to promote skin aging, headaches, acne and skin diseases. If constipation is severe, it can cause hemorrhoids such as breakage of dentition and escape of hemorrhoids during defecation, and severe colon cancer.

Meanwhile, the functional components of mulberry leaves include GABA (γ-amino butyrate), 1-dioxin, nozarymycin (DNJ, 1-deoxynojirimycin), rutin, etc. , Diabetes prevention, thrombolysis, obesity control, digestive function improvement, blood purification, stroke prevention.

As such, it is known that there are many active ingredients in mulberry leaves, but its composition and structure are still unknown. In particular, 1-dioxine and nozamycin of mulberry leaves are known to inhibit α-glucosidase and to increase blood sugar levels as well as to improve intestinal action and bowel movement.

The present invention was conceived by recognizing the above point, the object of the present invention is to increase the effect of improving constipation of mulberry leaves mulberry leaf powder mixed with mulberry leaf powder composition prepared by mixing green tea powder and chlorella powder, mulberry leaf powder lactic acid bacteria By fermentation as a starter to provide a mulberry leaf powder fermentation composition improved digestion and improved constipation efficacy.

In order to achieve the above object, the mixed mulberry leaf powder composition according to the present invention is characterized in that 5% by weight of green tea powder and 5% by weight of chlorella powder are mixed with 90% by weight of mulberry leaf powder.

In addition, the mulberry leaf powder fermentation composition according to the present invention is characterized in that the mulberry leaf powder was inoculated and fermented by lactic acid bacteria starter.

In addition, the mulberry leaf powder fermentation composition according to the present invention, the mulberry leaf powder is 90% by weight, lactobacillus starter is the casei lactic acid bacteria ( Lactobacillus casei ) Lactobacillus 5% by weight reuteri ) 5% by weight, the fermentation is characterized in that the fermented for about 5 days at 35 ℃.

Hereinafter, the mixed mulberry leaf powder composition and the fermentation composition according to the present invention will be described in detail with reference to Examples.

Example 1 Preparation of Mixed Mulberry Leaf Powder Composition and Mulberry Leaf Powder Fermentation Composition

1. Preparation of mixed mulberry leaf powder composition

10Kg of fresh mulberry leaves were chopped into 3cm x 3cm size and then ground into particles of 80 mesh using a pin crusher. The pulverized mulberry leaf powder was sterilized in an autoclave at 121 ° C. for 30 minutes and then cooled to room temperature. First, mixed with mulberry leaf powder 90% by weight of 5% by weight of green tea and 5% by weight of chlorella and then stirred for 30 minutes using a ribbon mixer to prepare a mixed mulberry leaf powder composition.

2. Lactobacillus ( Lactobacillus) as a lactobacillus starter pre-cultured in MRS medium to 90% by weight of the sterilized mulberry leaf powder as described above casei ) Lactobacillus 5% by weight reuteri ) 5% by weight inoculation and fermentation at 35 ℃ for 5 days to prepare a powder mulberry leaf powder fermentation composition.

Example 2 Experimental Animal

Forty-eight male Sprague-Dawley species (average 305-310 g) of 8-week-old male rats were used. The animals were placed in complete batches, separated by one, and reared for four weeks. The environmental temperature of the experimental animal breeding room was maintained at 22 ± 1 ℃ and the relative humidity was 65 ± 5%, and the contrast was adjusted by a 12 hour period (09: 00-21: 00). During the entire experiment, water and feed were ad libitum was used, and dietary intake and body weight were measured at regular times every week.

Loperamide, a constipation-causing substance, was used by Sigma (St. Louise, Mo., USA), and other reagents were used as general-purpose reagents.

Example 3 Experimental Dietary Composition

The diet used in the experiment was AIN-93 purified dietary composition, the dietary composition is shown in Table 1 below. Constipation-causing loperamide was not added to the normal control group (NC), and the control group (C), mixed mulberry leaf powder composition (MPA) and mulberry leaf powder fermentation composition (MPB) was set as the test group. Experimental diet was normal control (NC), control (C), mixed mulberry leaf powder composition 5% addition group (MPA5) and 10% addition group (MPA10), mulberry leaf powder fermentation composition 5% addition group (MPB5) and 10% addition group (MPB10) was divided into 6 groups and paid for 31 days. For 3 days from the 29th to 30th day of experiment, loperamide (2 mg / kg, sc) was dissolved in 0.5% Tween 20 and administered subcutaneously 3 times a day for 3 days from the 29th to 30th day of the experiment. Constipation was induced and sacrificed on day 32.

Diet composition (g / Kg) Groups ^{1 )} NC C MPA5 MPA10 MPB5 MPB10 Corn starch 400.0 400.0 350.0 300.0 350.0 300.0 Sucrose 200.0 200.0 200.0 200.0 200.0 200.0 Casein 200.0 200.0 200.0 200.0 200.0 200.0 L-cystine   3.0   3.0   3.0   3.0   3.0   3.0 Beef tallow 100.0 100.0 100.0 100.0 100.0 100.0 Cellulose  50.0  50.0  50.0  50.0  50.0  50.0 Vitamin mixture ^{2 )}  10.0  10.0  10.0  10.0  10.0  10.0 Mineral mixture ^{3 )}  35.0  35.0  35.0  35.0  35.0  35.0 Choline chloride   2.0   2.0   2.0   2.0   2.0   2.0 Mulburry mixture   0.0   0.0  50.0 100.0   0.0   0.0 Fermented Mulburry tea   0.0   0.0   0.0   0.0  50.0 100.0

* ¹⁾ NC: normal control group, C: loperamide treatment group, MPA5: mixed mulberry leaf powder composition 5% + loperamide, MPA10: mixed mulberry leaf powder composition 10% + loperamide, MBP5: mulberry leaf powder fermentation composition 5% + loperamide, MBP10: mulberry leaf Powder fermentation composition 10% + loperamide.

* ^{2), 3)} AIN-93-VX vitamin mixture, AIN-93-MX mineral mixture.

Example 4 Dietary Intake, Weight Gain and Feed Efficiency

Body weight and feed intake of test animals were measured every two days before loperamide administration (before constipation, Day 1-28 days), and at a specific time during loperamide administration period (constipation induction period, Day 29-31). It was. Feed efficiency was calculated by dividing the weight gain during the breeding period by the dietary intake consumed during the same period.

Feed intake, weight gain and feed efficiency of each group during the experimental period are shown in Table 2. Body weight gain and dietary intake were increased before and after the administration of loperamide. Only the group showed significant difference. There was no significant difference in dietary efficiency between treatment groups. The weight gain during the pre-experiment period tended to decrease significantly as the level of all treatments increased.

Day Groups ^{1 )} NC C MPA5 MPA10 MPB5 MPB10 Weight gain (g / day) 1-28 3.49 ± 0.49 ³⁾ 3.51 ± 0.23 3.11 ± 0.22 2.87 ± 0.09 3.24 ± 0.17 2.99 ± 0.34 29-31 3.50 ± 0.21 3.57 ± 0.27 3.22 ± 0.15 3.06 ± 0.31 ^{* #} 3.20 ± 0.16 2.98 ± 0.21 ^{* #} Feed Intake (g / day) 1-28 23.32 ± 0.29 23.25 ± 0.91 20.79 ± 0.25 19.23 ± 0.84 22.24 ± 0.01 20.14 ± 0.97 29-31 25.11 ± 0.01 23.34 ± 0.18 21.13 ± 0.87 19.17 ± 0.90 ^{* #} 23.54 ± 0.87 19.01 ± 0.75 ^{* #} Dietary Efficiency ^{2 )} 1-28 0.15 ± 0.03 0.15 ± 0.07 0.15 ± 0.03 0.15 ± 0.02 0.15 ± 0.04 0.15 ± 0.08 29-31 0.14 ± 0.09 0.15 ± 0.04 0.15 ± 0.04 0.15 ± 0.06 0.14 ± 0.03 0.15 ± 0.04

* ¹⁾ See Table 1.

** ²⁾ Food efficiency ratio (FER): weight gain / feed intake.

*** ³⁾ Each value is mean ± SE (Standard Error) for 8 animals in each group.

**** ^* (<0.05 vs NC) significant difference, ^# (<0.05 vs C) significant difference.

Example 5 Chapter Passing Time and Speed

The digestive tract transit time of the diet was fasted for 12 hours on the 3rd day after loperamide was administered to the test animal, and then fed with 1 g of 10% coomassie brilliant blue dye to the diet. The intestinal pain and velocity were calculated by dividing the length of the intestine by the passage time.

Influence on intestinal transit time, transit rate and intestinal length according to the intake of mulberry leaf powder fermentation composition is shown in Table 3.

The effect on intestinal transit time was significantly decreased in MPA and MPB compared to loperamide alone control (C). Longer transit time was reduced in MPB compared to MPA.

Groups ^{1 )} Intestine length (cm) Transit time (min) Transit speed (mm / min) NC 126.8 ± 0.29 ²⁾ 629.0 ± 9.46 ^# 2.09 ± 0.98 C 127.2 ± 5.20 695.7 ± 5.81 ^* 2.46 ± 0.92 ^* MPA5 136.2 ± 2.94 645.1 ± 1.08 ^# 2.15 ± 0.84 ^# MPA10 143.6 ± 8.91 ^* 621.2 ± 4.90 ^# 2.09 ± 0.79 ^# MPB5 137.0 ± 1.08 635.9 ± 9.41 ^# 2.11 ± 0.29 ^# MPB10 149.9 ± 0.08 ^* 617.2 ± 9.47 ^# 2.02 ± 0.51 ^#

* ¹⁾ See Table 1.

** ²⁾ Each value is mean ± SE (Standard Error) for 8 animals in each group.

*** ^* (<0.05 vs NC) significant difference, ^# (<0.05 vs C) significant difference.

Example 5 Measurement of the Number, Length, and Length of Intestinal Edges

The number of intestinal stools was sacrificed on the third day after loperamide administration, and the entire area from the cecum to the rectum was taken and fixed with 10% formaldehyde, and the number of bowels in the colon was observed. After measuring the length of each), the incision was measured and each weight except the contents was measured.

After feeding MPA and MPB to experimental animals while inducing constipation by administering loperamide, the number of stools in the colon was observed. Decreased. These results suggested that constipation could be caused by decreased bowel motility in loperamide-treated rats. MPA and MPB contributed to shortening of dietary intestinal pain and time, and reduced the number of feces in the colon. .

The intestinal length was significantly longer in the MPA and MPB treatment groups than in the normal control or loperamide treatment groups, and increased with increasing levels.

MPA and MPB actively promote the peristalsis of the intestine, so the length of the small intestine and large intestine increases, the volume of the intestinal contents increases and the intestinal muscles develop to shorten the intestinal transit time.

Groups ^{1 )} No. of fecal pellets Ratio (%) NC 4.34 ± 0.71 ^{2) #} 70 C 6.24 ± 0.62 ^* 100 MPA5 4.11 ± 0.40 ^# 66 MPA10 3.51 ± 0.81 ^{* #} 56 MPB5 3.91 ± 0.99 ^# 63 MPB10 3.29 ± 0.94 ^{* #} 53

* ¹⁾ See Table 1.

** ²⁾ Each value is mean ± SE (Standard Error) for 8 animals in each group.

*** ^* (<0.05 vs NC) significant difference, ^# (<0.05 vs C) significant difference.

Example 6 Number of Stools, Weight of Stools and Water Content of Stools

The number of feces, weight of feces and moisture content of feces were calculated by dividing into two parts before constipation and constipation period. The stools of each experimental animal were collected four times at the start of the experiment and at intervals of seven days thereafter. During the constipation-inducing period, the stools were collected daily and the number of stools per individual was measured. The moisture content of the stool was calculated by drying the stools in a 70 ℃ oven for 24 hours to measure the dry weight and dividing the difference between the stool weight and dry weight divided by the stool weight. Table 5 summarizes the number of sides, weight of the sides and the moisture content of the sides.

There was no significant difference in the number of feces before the administration of loperamide (up to 20 days of the experiment), but the amount of defecation and moisture content in the treated groups with MPA and MPB were higher than those in the control group (NC and C). Higher trends were seen. The number and defecation of stool in the experimental animals after loperamide administration for constipation were increased as the level of MPA and MPB was increased. Loperamide is used to induce constipation in experimental animals because it prolongs defecation time by inhibiting drugs or bowel movements used for diarrhea treatment. In this study, the administration of loperamide resulted in the decrease of the number of feces, the weight of the feces, and the decrease of the water content of the feces.

Day Groups ^{1 )} NC C MPA5 MPA10 MPB5 MPB10 Number of sides (count / day)  1-28 62.23 ± 0.96 65.13 ± 0.09 66.14 ± 0.10 64.09 ± 0.15 63.29 ± 0.67 67.59 ± 0.01 29-31 63.41 ± 0.26 ^# 42.13 ± 0.87 ^* 56.26 ± 0.29 ^# 61.29 ± 0.01 ^# 59.11 ± 0.01 ^# 63.29 ± 0.27 ^# Stool weight (g / day)  1-28 9.97 ± 0.97 9.15 ± 0.02 12.46 ± 0.21 14.39 ± 0.21 ^# 13.21 ± 0.28 16.39 ± 0.01 ^# 29-31 10.01 ± 0.23 ^# 6.31 ± 0.98 ^* 13.33 ± 0.76 ^# 13.99 ± 0.53 ^# 14.00 ± 0.94 ^# 15.21 ± 0.30 ^# Water content of feces (%)  1-28 35.23 ± 0.21 35.91 ± 0.10 39.62 ± 0.24 45.14 ± 0.26 ^# 38.13 ± 0.15 46.29 ± 0.01 ^# 29-31 36.13 ± 0.15 31.02 ± 0.29 35.14 ± 0.87 39.84 ± 0.13 ^# 37.21 ± 0.76 40.14 ± 0.18 ^#

* ¹⁾ See Table 1.

** ²⁾ Each value is mean ± SE (Standard Error) for 8 animals in each group.

*** ^* (<0.05 vs NC) significant difference, ^# (<0.05 vs C) significant difference.

The mixed mulberry leaf powder composition and mulberry leaf powder fermentation composition according to the present invention by the configuration as described above has the effect as a functional composition for improving constipation by reducing the intestinal transit time of the diet to improve constipation, powdered tea or capsule, It can be utilized as a dietary supplement food material.

The mixed mulberry leaf powder composition and the mulberry leaf powder fermentation composition described above are just one embodiment for carrying out the present invention, and should not be construed as limiting the technical spirit of the present invention. The scope of protection of the present invention is defined only by the matters set forth in the claims below, and the embodiments which have been improved and changed without departing from the gist of the present invention will be apparent to those skilled in the art. It will be said to belong to the protection scope of the present invention.

Claims (3)

delete delete Lactobacillus starter is inoculated into mulberry leaf powder to ferment, Mulberry leaf powder is 90% by weight, Lactobacillus starter is 5% by weight of Lactobacillus casei and 5% by weight of Lactobacillus reuteri , Fermentation mulberry leaf powder fermentation composition, characterized in that the fermentation for about 5 days at 35 ℃.