CN114558038B - Probiotic composition for losing weight and reducing fat as well as preparation method and application thereof - Google Patents

Probiotic composition for losing weight and reducing fat as well as preparation method and application thereof Download PDF

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CN114558038B
CN114558038B CN202210189384.0A CN202210189384A CN114558038B CN 114558038 B CN114558038 B CN 114558038B CN 202210189384 A CN202210189384 A CN 202210189384A CN 114558038 B CN114558038 B CN 114558038B
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weight
parts
probiotic
probiotic composition
fat
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CN114558038A (en
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王冠
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Bafeiba Food Technology Co ltd
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Guangzhou Bafeiba Health Industry Co ltd
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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Abstract

The invention belongs to the technical field of probiotics, and particularly relates to a probiotic composition for losing weight and reducing fat, and a preparation method and application thereof. The invention provides a probiotic composition for losing weight and reducing fat, which comprises the following components in parts by weight: 10-30 parts of composite probiotic powder, 15-25 parts of plant active ingredients, 8-16 parts of auxiliary protective agents, 20-30 parts of filling agents and 1-6 parts of sweeteners. The probiotic composition provided by the invention has the advantages that the composite probiotic powder and the plant active ingredients are scientifically and reasonably matched for use, so that the generation of fat in a human body can be effectively reduced, and meanwhile, the types and the quantity of probiotic bacteria can be increased, and the growth of harmful bacteria can be inhibited, so that the effect of selectively regulating intestinal flora is achieved, the stability and the weight-losing effect of the probiotic composition are improved, the weight-losing effect is obvious, and the probiotic composition is suitable for a wide range of people.

Description

Probiotic composition for losing weight and reducing fat as well as preparation method and application thereof
Technical Field
The invention belongs to the technical field of probiotics, and particularly relates to a probiotic composition for losing weight and reducing fat, and a preparation method and application thereof.
Background
Nowadays, the problem of obesity becomes a global social problem, and obesity is mainly caused by metabolic diseases caused by excessive accumulation of fat in the body due to disorder of energy metabolism balance of the body, which results in greater energy intake than energy consumption. The harm to human beings caused by obesity is not small, not only the psychological burden is increased to the obese people, but also psychological disorder can be caused by serious people, and the obesity is closely related to diseases such as hyperlipidemia, hypertension, diabetes, insulin resistance, hyperuricemia, nonalcoholic fatty liver, coronary atherosclerotic heart disease and the like. It can be seen that obesity and its complications have become a major public health problem worldwide, affecting not only the external signs of individuals, but also causing serious harm to human health.
For intervention and treatment of obesity, the life style intervention combined with the current exercise therapy and diet therapy is more effective and is most favored, but the efficiency is low, the period is long, and the patient is difficult to adhere to; surgical intervention is also one of the effective weight loss means, but often has high risk and high price, and is difficult for patients to bear; in addition, the efficacy of the drug treatment is high, the effect is good, the risk is low, and the patient is easy to accept, but some drugs may have severe adverse reactions to the patient, so that development of novel natural lipid-lowering drugs for preventing and treating obesity is urgently needed.
At present, a great deal of research shows that obesity is also closely related to the intestinal microbiota. The probiotics can reduce serum total cholesterol and triglyceride levels of obesity model mice induced by high-fat diet, improve lipid metabolism, reduce fat accumulation in vivo, and reduce body weight, thereby reducing the risk of obesity-related diseases. For example, chinese patent application CN 112544967A discloses a fat-reducing prebiotic composite probiotic dietary supplement, which comprises 40-50 parts of inulin, 25-35 parts of polydextrose, 15-25 parts of probiotic fermented fruit and vegetable powder, 10-15 parts of arabinose, 5-15 parts of citrus fruit powder, 4-6 parts of L-carnitine, 1-5 parts of lotus leaf powder and 0.5-3 parts of lactobacillus plantarum TWK. In theory, each component of the invention can synergistically inhibit fat absorption and promote fat decomposition, but can not resist the severe environment of the gastrointestinal tract, so that the number of viable probiotics reaching the hindgut is greatly reduced, and the effects of intestinal tract regulation, weight reduction and lipid reduction are greatly reduced.
Therefore, a probiotic preparation needs to be developed, which can effectively improve the activity of probiotics after digestion in the gastrointestinal tract and can improve the effects of losing weight and reducing fat.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a probiotic composition for losing weight and reducing fat. The probiotic composition provided by the invention has the advantages that the composite probiotic powder and the plant active ingredients are scientifically and reasonably matched for use, so that the generation of fat in a human body can be effectively reduced, and meanwhile, the types and the quantity of probiotic bacteria can be increased, and the growth of harmful bacteria can be inhibited, so that the effect of selectively regulating intestinal flora is achieved, the stability and the weight-losing effect of the probiotic composition are improved, the weight-losing effect is obvious, and the probiotic composition is suitable for a wide range of people.
The technical scheme of the invention is as follows:
the probiotic composition for losing weight and reducing fat comprises the following components in parts by weight:
10-30 parts of composite probiotic powder, 15-25 parts of plant active ingredients, 8-16 parts of auxiliary protective agents, 20-30 parts of filling agents and 1-6 parts of sweeteners;
the compound probiotic powder is a combination of two or more than two of bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans, lactobacillus casei, lactobacillus acidophilus and lactobacillus bulgaricus; further, the viable count of each probiotic is more than or equal to 1X 109CFU/g;
the plant active ingredient is the combination of two or more of chlorogenic acid, bamboo leaf flavone and pachyman.
Further, the plant active ingredients comprise chlorogenic acid, bamboo leaf flavone and pachyman in a weight ratio of 3-7:1-5:11-16.
The invention takes the composite probiotics as the main raw material, through the ingestion of probiotics by human body, the invention can increase the planting capacity and planting time of endogenous and exogenous probiotics in human intestinal tracts while increasing the types of intestinal probiotics, inhibit the growth and propagation of intestinal harmful bacteria, especially gram negative bacteria, fully regulate the composition of the intestinal probiotics, regulate the structure of the intestinal flora, relieve obesity symptoms, and have some functional activities, health effect, transport activity and certain gastrointestinal adhesiveness, and also have the functions of inhibiting intestinal pathogenic bacteria and regulating immunity.
The invention also combines natural plant active ingredients, wherein the added chlorogenic acid has specific inhibition effect on glucose-6-phosphatase, can reduce the output of hepatic glucose, inhibit the absorption of glucose by small intestine, reduce the glucose transport capacity by 80% by 1mM chlorogenic acid, reduce the absorption of glucose by organism, thereby reducing the formation of fat. Experiments prove that when chlorogenic acid is compounded with bamboo leaf flavone and pachyman, the weight of obese rats can be obviously reduced, and presumably the compounded plant active ingredient has a strong physiological activity function and a certain positive effect on promoting the growth of probiotics, so that the proliferation of the probiotics can be promoted, the probiotics function can be better played, meanwhile, the dysbacteriosis of intestinal canal can be corrected, and the bacteria causing obesity can be reduced or eliminated.
Further, the probiotic composition for losing weight and reducing fat comprises the following components: 25 parts of composite probiotic powder, 20 parts of plant active ingredients, 12 parts of auxiliary protective agent, 23 parts of filler and 4 parts of sweetener.
Further, the auxiliary protective agent consists of resveratrol and medium chain triglyceride according to the weight ratio of 7-11:1-4.
Still further, the auxiliary protectant consists of resveratrol and medium chain triglycerides in a weight ratio of 10:3.
Further, the filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to the weight ratio of 1-3:11-17:4-7.
Further, the filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to a weight ratio of 2:14:5.
Further, the sweetener is erythritol.
In addition, the invention also provides a preparation method of the probiotic composition for losing weight and reducing fat, which comprises the following steps:
s1, mixing composite probiotic powder and an auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 3-5 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding plant active ingredients and a sweetener after uniformly stirring, and continuously stirring for 10-15 min to obtain a mixture A;
s2, adding distilled water with the mass 2-4 times of that of the filler into the filler, and stirring for 1-3 min at the rotating speed of 800-1200 rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 1-2 h at the stirring speed of 4000-8000 rpm, adding into an extrusion microcapsule granulator for extrusion granulation, and finally freeze-drying the obtained particles.
Further, in the step S1, the stirring speed is 200-300 rpm, and the temperature during stirring is 25-30 ℃.
The probiotic composition prepared by the invention can be applied to medicines or health products with the weight-losing efficacy.
The added composite probiotic powder and plant active ingredients belong to active substances, are easily influenced by environmental factors such as temperature, humidity, time and the like in the preparation process, so that the activity of the prepared probiotic compound is damaged, the stability of the product is greatly reduced, and a plurality of probiotics are easily deactivated when orally taken through the strong acid environment of the stomach, so that the active effect exerted by the probiotics in intestinal tracts is weaker, and the regulating effect on intestinal tracts and weight and lipid reduction is also greatly reduced. In order to ensure the activity of probiotics in intestinal tracts to the maximum extent, the preparation process of the probiotic composition is improved, and the auxiliary protective agent consisting of resveratrol and medium-chain triglyceride according to a certain weight proportion is added simultaneously in the mixing process of materials, so that the influence of environmental factors in the preparation process on the probiotics and plant active ingredients of the composition can be effectively reduced; meanwhile, the probiotics and plant active ingredients are uniformly embedded by the added filler which is formed by compounding the galactooligosaccharide, the hydroxyethyl cellulose and the algin according to a certain proportion, so that the acid resistance of the probiotic composition can be effectively enhanced, the loss of the probiotics in the gastric acid digestion process can be effectively reduced, the probiotics are effectively protected from being corroded by acid, bile salt and digestive enzymes, and the stability of the probiotics is effectively maintained.
Compared with the prior art, the probiotic composition for losing weight and reducing fat has the following advantages:
(1) The probiotic composition provided by the invention is scientifically and reasonably matched with the plant active ingredients, can effectively reduce the generation of fat in the body, increase the types and the quantity of probiotic bacteria, increase the nutrition substances of the probiotic bacteria and inhibit the growth of harmful bacteria, thereby achieving the effect of selectively regulating intestinal flora, improving the probiotic property and the weight-losing effect of the probiotic composition, and has remarkable weight-losing effect and wide application range.
(2) The probiotic composition provided by the invention effectively reduces the loss of the activity of the probiotics and plant extract components caused by the environment in the preparation process through an improved preparation technology, and can also maintain the activity of the probiotics after entering the human digestive tract environment, reduce the influence of the human digestive tract environment on the activity of the probiotics, and effectively maintain the stability of the probiotics.
Detailed Description
The invention is further illustrated by the following description of specific embodiments, which are not intended to be limiting, and various modifications or improvements can be made by those skilled in the art in light of the basic idea of the invention, but are within the scope of the invention as long as they do not depart from the basic idea of the invention.
Bamboo leaf flavone, CAS number: 91771-33-4, available from Kafen Biotechnology Co., ltd; ginkgo flavone, pachyman, polyporus polysaccharide, are available from Fengshuote Biotechnology Co.
In addition, the reagents used in the invention are all common reagents and can be purchased by common reagent production and marketing companies.
Example 1A probiotic composition for weight and fat loss
The probiotic composition for losing weight and reducing fat comprises the following components in parts by weight:
10 parts of composite probiotic powder, 15 parts of plant active ingredients, 8 parts of auxiliary protective agent, 20 parts of filler and 1 part of erythritol:
the composite probiotic powder is prepared from bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans and lactobacillus acidophilus according to a weight ratio of 2:1:1:1, the composition is as follows; the viable count of each probiotic is more than or equal to 1X 109CFU/g.
The plant active ingredients consist of chlorogenic acid, bamboo leaf flavone and pachyman according to the weight ratio of 3:1:11.
The auxiliary protective agent consists of resveratrol and medium chain triglyceride according to a weight ratio of 7:4.
The filler is prepared from galacto-oligosaccharide, hydroxyethyl cellulose and algin according to a weight ratio of 1:17:7.
The preparation method of the probiotic composition for losing weight and reducing fat comprises the following steps:
s1, mixing composite probiotic powder and an auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 3 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding plant active ingredients and erythritol after uniformly stirring, and continuously stirring for 10min to obtain a mixture A;
s2, adding distilled water with the mass 2 times of that of the filler into the filler, and stirring for 1min at the rotating speed of 800rpm to obtain a mixture B;
s3, adding the mixture A into the mixture B, stirring for 1h at the stirring speed of 4000rpm, adding into an extrusion type microcapsule granulator for extrusion granulation treatment, and finally freeze-drying the obtained particles to obtain the composite material.
In the step S1, the stirring speed was 200rpm, and the stirring temperature was 25 ℃.
Example 2A probiotic composition for weight and fat loss
The probiotic composition for losing weight and reducing fat comprises the following components in parts by weight: 25 parts of composite probiotic powder, 20 parts of plant active ingredients, 12 parts of auxiliary protective agents, 23 parts of filling agents and 4 parts of erythritol.
The composite probiotic powder is prepared from bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans and lactobacillus acidophilus according to a weight ratio of 2:1:1:1, the composition is as follows; the viable count of each probiotic is more than or equal to 1X 109CFU/g.
The plant active ingredients consist of chlorogenic acid, bamboo leaf flavone and pachyman according to the weight ratio of 5:3:14.
The auxiliary protective agent consists of resveratrol and medium chain triglyceride according to the weight ratio of 10:3.
The filler is prepared from galactooligosaccharide, hydroxyethyl cellulose and algin according to a weight ratio of 2:14:5.
The preparation method of the probiotic composition for losing weight and reducing fat comprises the following steps:
s1, mixing composite probiotic powder and an auxiliary protective agent, then adding distilled water, wherein the adding amount of distilled water is 4 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding plant active ingredients and erythritol after uniformly stirring, and continuously stirring for 12min to obtain a mixture A;
s2, adding distilled water with the mass 3 times of that of the filler into the filler, and stirring for 2min at the rotating speed of 1000rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 1.5h at the stirring speed of 6000rpm, adding into an extrusion type microcapsule granulator for extrusion granulation, and finally freeze-drying the obtained particles.
In the step S1, the stirring speed was 250rpm, and the stirring temperature was 27 ℃.
Example 3A probiotic composition for weight and fat loss
The probiotic composition for losing weight and reducing fat comprises the following components in parts by weight:
30 parts of composite probiotic powder, 25 parts of plant active ingredients, 16 parts of auxiliary protective agent, 30 parts of filler and 6 parts of erythritol:
the composite probiotic powder is prepared from bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans and lactobacillus acidophilus according to a weight ratio of 2:1:1:1, the composition is as follows; the viable count of each probiotic is more than or equal to 1X 109CFU/g.
The plant active ingredients consist of chlorogenic acid, bamboo leaf flavone and pachyman according to the weight ratio of 7:1:11.
The auxiliary protective agent consists of resveratrol and medium chain triglyceride according to the weight ratio of 11:1.
The filler is prepared from galactooligosaccharide, hydroxyethyl cellulose and algin according to a weight ratio of 3:11:4.
The preparation method of the probiotic composition for losing weight and reducing fat comprises the following steps:
s1, mixing composite probiotic powder and an auxiliary protective agent, then adding distilled water, wherein the adding amount of distilled water is 5 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding plant active ingredients and erythritol after uniformly stirring, and continuously stirring for 15min to obtain a mixture A;
s2, adding distilled water with the mass 4 times of that of the filler into the filler, and stirring for 3min at the rotating speed of 1200rpm to obtain a mixture B;
s3, adding the mixture A into the mixture B, stirring for 2 hours at the stirring speed of 8000rpm, adding into an extrusion type microcapsule granulator for extrusion granulation treatment, and finally freeze-drying the obtained particles to obtain the composite material.
In the step S1, the stirring speed was 300rpm, and the stirring temperature was 30 ℃.
Comparative example 1A probiotic composition for weight and fat loss
In comparison with example 2, comparative example 1 is different in that pachymaran in the plant active ingredient is replaced with agaric polysaccharide, and other components and preparation steps are the same as example 2.
Comparative example 2A probiotic composition for weight and fat loss
Comparative example 2 is different from example 2 in that the bamboo leaf flavonoid in the plant active ingredient is replaced with ginkgo flavone, and other components and preparation steps are the same as example 2.
Comparative example 3A probiotic composition for weight and fat loss
In comparison with example 2, comparative example 3 is different in that the auxiliary protecting agent is replaced with D-isoascorbic acid, and other components and preparation steps are the same as those of example 2.
Comparative example 4A probiotic composition for weight and fat loss
Comparative example 4 differs from example 2 in that the medium chain triglyceride in the auxiliary protecting agent was replaced with linoleic acid, and other components and preparation steps were the same as example 2.
Comparative example 5A probiotic composition for weight and fat loss
Comparative example 5 differs from example 2 in that the galactooligosaccharide in the filler was replaced with the maltooligosaccharide, and other components and preparation steps were the same as in example 2.
Comparative example 6A probiotic composition for weight and fat loss
Comparative example 6 was different from example 2 in that the alginate in the filler was replaced with guar gum, and other components and preparation steps were the same as in example 2.
Test example I, weight-loss function evaluation experiment
1. Test materials: examples 1 to 3 and comparative examples 1 to 6.
2. The test method comprises the following steps:
(1) Establishing a rat obesity model: clean SD rat male adult rats with body weight range of 230-290 g and 110 free diet drinking water. Animals were kept in single cages and fed with normal feed for 5 days, and after the adaptation period was completed, the animals were randomly divided into 2 groups according to the body weight of the rats, 10 rats were fed with normal feed as a blank group, and 100 rats were fed with high calorie feed as a model group. Recording feed intake, food intake and residual food amount every week, weighing body weight for 1 time, continuously feeding for 2 weeks, and completing establishment of rat obesity model; the high-calorie model feed is prepared according to the requirements of national food and medicine supervision [2012]107 annex 8 'weight-losing function evaluation method'.
(2) Administering a test agent: rats in a model group are divided into an example 1-3 group, a comparative example 1-6 group and a model control group, 10 rats in each group are respectively added into a high-calorie model feed in the test group by the probiotic composition prepared in the example 1-3 group and the comparative example 1-6, the addition amount is 3%, and the model control group is only fed with the high-calorie model feed. The test subjects were given 6 weeks. The feed and the residual feed were recorded weekly, and the body weight was weighed 1 time.
(3) Index detection and recording: after the last sample administration of the rats, 16 hours after fasting, the rats were weighed, anesthetized with 1% pentobarbital sodium [0.5 mL/(100 g.bw) ] and the perirenal fat, peritesticular fat, was dissected and weighed to calculate the fat/body ratio. In addition, rat weight gain, in vivo fat weight (testicle and perirenal fat pad) and fat/body weight were counted.
3. Test results
The test results are shown in Table 1.
TABLE 1 Effect of the invention on rat body weight and fat weight
Group of Average initial weight (g) Average final weight (g) Average weight gain (g) Fat weight in vivo (g) Fat/body ratio (%)
Blank control group 354.1 469.4 115.3 11.3 2.41%
Model control group 383.1 558.7 175.6 24.7 4.42%
Example 1 group 381.7 509.0 127.3 13.8 2.71%
Example 2 group 381.0 505.2 124.2 13.1 2.59%
Example 3 group 381.1 507.8 126.7 14.2 2.80%
Comparative example 1 group 381.4 528.9 147.5 16.8 3.18%
Comparative example 2 group 382.6 537.7 155.1 17.7 3.29%
Comparative example 3 group 380.2 517.4 137.2 15.9 3.07%
Comparative example 4 group 382.8 515.3 132.5 15.1 2.93%
Comparative example 5 group 381.5 514.9 133.4 14.8 2.87%
Comparative example 6 group 384.5 534.2 149.7 15.7 2.94%
As can be seen from Table 1, after the probiotic compositions prepared in the groups 1 to 3 of the present invention were fed for 6 weeks, the average final weight, weight gain and body fat weight were significantly reduced as compared with the model control group, wherein the group 2 had the best effect and the fat/body ratio was only 2.59%, which is the best example of the present invention. While the average weight gain, in vivo fat weight and fat/body comparison example group of the obese mice of comparative examples 1 to 6 were significantly increased.
Test example two, artificial gastric juice tolerance test
1. Test materials: the probiotic compositions for weight and fat loss prepared in examples 1 to 3 and comparative examples 1 to 6.
2. The test method comprises the following steps: respectively taking 0.2g of the probiotic composition prepared in examples 1-3 and comparative examples 1-6, placing the probiotic composition in 10mL of artificial simulated gastric fluid, vibrating and uniformly mixing, incubating in a 37 ℃ water bath, sampling every 30min, placing the probiotic composition in a 0.06mol/L sodium citrate solution, vibrating and treating for 1h (35 ℃ and 100 rpm) to fully dissolve the composition, performing viable count by adopting a plate counting method, and calculating the survival rate of the probiotic composition.
The formula of the artificial simulated gastric juice is as follows: 2g of sodium chloride and 3.2g of pepsin are taken and dissolved in 7ml of 1mol/L HCl solution, water is added to 1000ml, and after preparation, the pH value is measured to be about 2.0.
3. Test results
The survival rates of probiotics in simulated gastric fluid in the compositions prepared in examples 1-3 and comparative examples 1-6 of the present invention are shown in Table 2.
TABLE 2
As can be seen from table 2, the probiotic compositions prepared in examples 1 to 3 of the present invention show good gastric environment tolerance in the case of gastric acid simulation, and the survival rate of the probiotic can still reach more than 89% after 2 hours in gastric juice simulation, wherein the best effect of example 2 is the best example of the present invention. In comparative examples 3 to 6, the survival rate of the probiotics in the obtained composition in simulated gastric fluid is reduced to different degrees when the components of the auxiliary protective agent and the filler are changed. The probiotic composition prepared by the components and the preparation method can strengthen the acid resistance of the probiotic composition and protect the probiotic composition from being damaged in the gastric digestion process.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.

Claims (7)

1. The probiotic composition for losing weight and reducing fat is characterized by comprising the following components in parts by weight:
10-30 parts of composite probiotic powder, 15-25 parts of plant active ingredients, 8-16 parts of auxiliary protective agents, 20-30 parts of filling agents and 1-6 parts of sweeteners;
the composite probiotic powder is prepared from bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans and lactobacillus acidophilus according to a weight ratio of 2:1:1:1, the composition is as follows;
the plant active ingredients consist of chlorogenic acid, bamboo leaf flavone and pachyman in a weight ratio of 3-7:1-5:11-16;
the auxiliary protective agent consists of resveratrol and medium chain triglyceride according to the weight ratio of 7-11:1-4;
the filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to the weight ratio of 1-3:11-17:4-7.
2. The probiotic composition for weight and fat loss according to claim 1, which is composed of the following components in parts by weight: 25 parts of composite probiotic powder, 20 parts of plant active ingredients, 12 parts of auxiliary protective agent, 23 parts of filler and 4 parts of sweetener.
3. The probiotic composition for weight and fat loss according to claim 1, wherein the auxiliary protectant consists of resveratrol and medium chain triglycerides in a weight ratio of 10:3.
4. The probiotic composition for weight and fat loss according to claim 1, wherein the filler is composed of galactooligosaccharide, hydroxyethyl cellulose and algin in a weight ratio of 2:14:5.
5. The probiotic composition for weight and fat loss according to claim 1 or 2, wherein the sweetener is erythritol.
6. A method for preparing a probiotic composition for weight loss and fat loss according to any one of claims 1 to 5, comprising the steps of:
s1, mixing composite probiotic powder and an auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 3-5 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding plant active ingredients and a sweetener after uniformly stirring, and continuously stirring for 10-15 min to obtain a mixture A;
s2, adding distilled water with the mass 2-4 times of that of the filler into the filler, and stirring for 1-3 min at the rotating speed of 800-1200 rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 1-2 h at the stirring speed of 4000-8000 rpm, adding into an extrusion microcapsule granulator for extrusion granulation, and finally freeze-drying the obtained particles.
7. The method for preparing a probiotic composition for weight and fat loss according to claim 6, wherein the stirring speed in the step S1 is 200 to 300rpm, and the stirring temperature is 25 to 30 ℃.
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