CN114558038A - Probiotic composition for losing weight and reducing fat and preparation method and application thereof - Google Patents

Probiotic composition for losing weight and reducing fat and preparation method and application thereof Download PDF

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CN114558038A
CN114558038A CN202210189384.0A CN202210189384A CN114558038A CN 114558038 A CN114558038 A CN 114558038A CN 202210189384 A CN202210189384 A CN 202210189384A CN 114558038 A CN114558038 A CN 114558038A
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weight
parts
probiotic
probiotic composition
fat
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CN114558038B (en
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王冠
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Bafeiba Food Technology Co ltd
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Guangzhou Mujia Health Industry Co ltd
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    • A61K35/66—Microorganisms or materials therefrom
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    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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    • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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Abstract

The invention belongs to the technical field of probiotics, and particularly relates to a probiotic composition for losing weight and reducing fat, and a preparation method and application thereof. The invention provides a probiotic composition for reducing weight and fat, which comprises the following components in parts by weight: 10-30 parts of composite probiotic powder, 15-25 parts of plant active ingredients, 8-16 parts of auxiliary protective agents, 20-30 parts of fillers and 1-6 parts of sweetening agents. The probiotic composition provided by the invention scientifically and reasonably uses the composite probiotic powder and the plant active ingredients in a matching manner, can effectively reduce the generation of fat in vivo, and can increase the types and the quantity of probiotic strains and inhibit the growth of harmful bacteria, thereby achieving the selective regulation effect on intestinal flora, improving the stability and the weight-losing effect of the probiotic composition, having remarkable weight-losing effect and being suitable for wide crowds.

Description

Probiotic composition for losing weight and reducing fat as well as preparation method and application thereof
Technical Field
The invention belongs to the technical field of probiotics, and particularly relates to a probiotic composition for losing weight and reducing fat, and a preparation method and application thereof.
Background
Nowadays, the obesity problem becomes a global social problem, and is mainly caused by metabolic diseases caused by the fact that the energy intake is larger than the energy consumption due to the disorder of the energy metabolism balance of the body, and the excessive accumulation of fat in the body is caused. The harm brought by obesity to human beings is not only increased in psychological burden brought to obese people, but also psychological disorders caused by serious people, and is closely related to diseases such as hyperlipidemia, hypertension, diabetes, insulin resistance, hyperuricemia, non-alcoholic fatty liver disease, coronary atherosclerotic heart disease and the like. It is seen that obesity and its complications have become a major public health problem worldwide, affecting not only the individual's physical signs, but also causing serious harm to human health.
For the intervention and treatment of obesity, the current lifestyle intervention combining exercise therapy and diet therapy is more effective and advocated, but the efficiency is low, the period is long, and patients are difficult to insist; surgical intervention is also one of the effective means of weight loss, but often has high risk and is expensive, difficult for patients to bear; in addition, the medicine treatment efficiency is high, the effect is good, the risk is low, and the medicine is easy to accept by patients, but some medicines may have severe adverse reactions to the patients, so that the development of novel natural ingredient lipid-lowering medicines for preventing and treating obesity is urgently needed.
At present, a great deal of research shows that obesity is also closely related to intestinal microbial flora. The probiotics can reduce the serum total cholesterol and triglyceride level of obesity model mice induced by high fat diet, improve lipid metabolism, reduce fat accumulation in vivo, reduce weight, thereby reducing the risk of obesity-related diseases. For example, Chinese patent application CN 112544967A discloses a fat-reducing prebiotic composite probiotic dietary supplement, which comprises 40-50 parts of inulin, 25-35 parts of polydextrose, 15-25 parts of probiotic fermented fruit and vegetable powder, 10-15 parts of arabinose, 5-15 parts of citrus fruit powder, 4-6 parts of L-carnitine, 1-5 parts of lotus leaf powder and 100.5-3 parts of lactobacillus plantarum TWK. The components of the invention can synergistically inhibit fat absorption and promote fat decomposition in theory, but cannot resist the severe environment of the gastrointestinal tract, so that the number of viable probiotics reaching the rear intestine is greatly reduced, and the regulating effects of the composition on intestinal regulation and weight and fat reduction are greatly reduced.
Therefore, there is a need to develop a probiotic preparation, which can effectively improve the activity of probiotics after digestion in gastrointestinal tract, and can improve the effects of reducing weight and reducing fat.
Disclosure of Invention
In order to solve the problems in the prior art, the present invention aims to provide a probiotic composition for weight and fat reduction. The probiotic composition provided by the invention scientifically and reasonably uses the composite probiotic powder and the plant active ingredients in a matching manner, can effectively reduce the generation of fat in vivo, and can increase the types and the quantity of probiotic strains and inhibit the growth of harmful bacteria, thereby achieving the selective regulation effect on intestinal flora, improving the stability and the weight-losing effect of the probiotic composition, having remarkable weight-losing effect and being suitable for wide crowds.
The technical scheme of the invention is as follows:
a probiotic composition for losing weight and reducing fat comprises the following components in parts by weight:
10-30 parts of composite probiotic powder, 15-25 parts of plant active ingredients, 8-16 parts of auxiliary protective agents, 20-30 parts of fillers and 1-6 parts of sweetening agents;
the composite probiotic powder is a combination of two or more of bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans, lactobacillus casei, lactobacillus acidophilus and lactobacillus bulgaricus; furthermore, the viable count of each probiotic is more than or equal to 1 multiplied by 109 CFU/g;
the plant active ingredients are two or more of chlorogenic acid, bamboo leaf flavone and pachyman.
Furthermore, the plant active ingredients comprise chlorogenic acid, bamboo leaf flavone and pachyman according to the weight ratio of 3-7: 1-5: 11-16.
The composite probiotics are used as main raw materials, and the intake of human body to probiotics can increase the species of intestinal probiotics flora, enhance the colonization ability and the colonization time of endogenous and exogenous probiotics in the intestinal tract of human body, inhibit the growth and the reproduction of harmful intestinal bacteria, especially gram-negative bacteria, fully regulate the composition of the intestinal probiotics flora, regulate the structure of the intestinal flora, relieve obesity symptoms, simultaneously have some functional activities, have health effects, transport activity and certain gastrointestinal tract adhesion, and also have the effects of inhibiting intestinal pathogenic bacteria and regulating immunity.
The invention also compounds natural plant active ingredients, wherein the added chlorogenic acid has specific inhibition effect on glucose-6-phosphatase, can reduce the output of glycogen and inhibit the absorption of glucose by small intestine, 1mM chlorogenic acid can reduce the glucose transport capacity by 80%, and reduce the absorption of glucose by organism, thereby reducing the formation of fat. Experiments prove that when the chlorogenic acid is compounded with the bamboo leaf flavone and the pachyman, the weight of obese rats can be remarkably reduced, and presumably, the compounded plant active ingredient has stronger physiological activity function and has a certain positive effect on promoting the growth of probiotics, so that the proliferation of the probiotics can be promoted, the probiotics function can be better exerted, and meanwhile, the imbalance of intestinal flora can be corrected, and the bacteria causing obesity can be reduced or eliminated.
Furthermore, the probiotic composition for losing weight and reducing fat comprises the following components: 25 parts of composite probiotic powder, 20 parts of plant active ingredients, 12 parts of auxiliary protective agent, 23 parts of filler and 4 parts of sweetening agent.
Further, the auxiliary protective agent consists of resveratrol and medium-chain triglyceride in a weight ratio of 7-11: 1-4.
Further, the auxiliary protective agent consists of resveratrol and medium-chain triglyceride in a weight ratio of 10: 3.
Further, the filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to a weight ratio of 1-3: 11-17: 4-7.
Furthermore, the filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to the weight ratio of 2:14: 5.
Further, the sweetener is erythritol.
In addition, the invention also provides a preparation method of the probiotic composition for losing weight and reducing fat, which comprises the following steps:
s1, mixing the composite probiotic powder and the auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 3-5 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, uniformly stirring, adding the plant active ingredient and the sweetening agent, and continuously stirring for 10-15 min to obtain a mixture A;
s2, adding distilled water with the mass 2-4 times that of the filler into the filler, and stirring for 1-3 min at the rotating speed of 800-1200 rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 1-2 hours at the stirring speed of 4000-8000 rpm, adding into an extrusion type microcapsule granulator, carrying out extrusion granulation treatment, and finally carrying out freeze drying on the obtained granules to obtain the microcapsule.
Further, in the step S1, the stirring speed is 200-300 rpm, and the stirring temperature is 25-30 ℃.
The probiotic composition prepared by the invention can be applied to medicines or health products with weight-losing efficacy.
The added composite probiotic powder and plant active ingredients belong to active substances and are easily influenced by environmental factors such as temperature, humidity, time and the like in the preparation process, so that the activity of the prepared probiotic compound is damaged, the stability of the product is greatly reduced, and many probiotics are easily inactivated when being orally taken through a strong acid environment of the stomach, so that the activity of the probiotics in the intestinal tract is weaker, and the regulation effect of the probiotics on the intestinal tract and the weight and fat reduction is greatly reduced. In order to ensure the activity of probiotics in intestinal tracts to the maximum extent, the preparation process of the probiotic composition is improved, and the auxiliary protective agent consisting of resveratrol and medium-chain triglyceride in a certain weight proportion is added in the mixing process of materials, so that the influence of environmental factors on the probiotics and plant active ingredients of the composition in the preparation process can be effectively reduced; meanwhile, the probiotics and the plant active ingredients are uniformly embedded by the added filler which is formed by compounding galacto-oligosaccharide, hydroxyethyl cellulose and algin according to a certain proportion, so that the acid resistance of the probiotic composition can be effectively enhanced, the loss of the probiotics in the gastric acid digestion process is effectively reduced, the probiotics is effectively protected from being corroded by acid, bile salt and digestive enzyme, and the stability of the probiotics is effectively maintained.
Compared with the prior art, the probiotic composition for losing weight and reducing fat provided by the invention has the following advantages:
(1) the probiotic composition provided by the invention scientifically and reasonably uses the composite probiotic powder and the plant active ingredients in a matching manner, so that the generation of fat in vivo can be effectively reduced, the types and the number of probiotics can be increased, the nutrient substances of the probiotics can be increased, and the growth of harmful bacteria can be inhibited, thereby achieving the selective regulation effect on intestinal flora, improving the probiotic and the weight-losing effect of the probiotic composition, having remarkable weight-losing effect and being suitable for wide crowds.
(2) The probiotic composition provided by the invention effectively reduces the loss of the activity of the environment on the probiotics and plant extract components in the preparation process through an improved preparation technology, can also keep the activity of the probiotics after entering the human digestive tract environment, reduces the influence of the human digestive tract environment on the activity of the probiotics, and effectively keeps the stability of the probiotics.
Detailed Description
The present invention is further illustrated by the following description of specific embodiments, which are not intended to limit the invention, and various modifications and improvements can be made by those skilled in the art based on the basic idea of the invention, but the invention is within the protection scope of the invention.
Bamboo leaf flavonoid, CAS No.: 91771-33-4, available from Kaifen Biotech, Guangzhou; ginkgetin, pachyman, and grifola from Fufeng Sinuote Biotech, Inc.
In addition, the reagents used in the invention are all common reagents and can be purchased from conventional reagent production and sale companies.
Example 1A probiotic composition for weight and fat reduction
The probiotic composition for losing weight and reducing fat comprises the following components in parts by weight:
10 parts of composite probiotic powder, 15 parts of plant active ingredients, 8 parts of auxiliary protective agent, 20 parts of filler, 1 part of erythritol:
the composite probiotic powder is prepared from bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans and lactobacillus acidophilus according to a weight ratio of 2: 1: 1:1, preparing a composition; the viable count of each probiotic is more than or equal to 1 multiplied by 109 CFU/g.
The plant active ingredients comprise chlorogenic acid, bamboo leaf flavone and pachyman according to the weight ratio of 3:1: 11.
The auxiliary protective agent consists of resveratrol and medium-chain triglyceride according to the weight ratio of 7: 4.
The filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to the weight ratio of 1:17: 7.
The preparation method of the probiotic composition for losing weight and reducing fat comprises the following steps:
s1, mixing the composite probiotic powder and the auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 3 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding the plant active ingredient and erythritol after uniformly stirring, and continuously stirring for 10min to obtain a mixture A;
s2, adding distilled water with the mass 2 times of that of the filler into the filler, and stirring for 1min at the rotating speed of 800rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 1h at the stirring speed of 4000rpm, adding into an extrusion type microcapsule granulator for extrusion granulation, and finally freeze-drying the obtained granules to obtain the microcapsule.
In the step S1, the stirring speed was 200rpm, and the temperature during stirring was 25 ℃.
Example 2A probiotic composition for weight and fat reduction
The probiotic composition for losing weight and reducing fat comprises the following components in parts by weight: 25 parts of composite probiotic powder, 20 parts of plant active ingredients, 12 parts of auxiliary protective agents, 23 parts of fillers and 4 parts of erythritol.
The composite probiotic powder is prepared from bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans and lactobacillus acidophilus according to a weight ratio of 2: 1: 1:1, preparing a composition; the viable count of each probiotic is more than or equal to 1 × 109 CFU/g.
The plant active ingredients comprise chlorogenic acid, bamboo leaf flavone and pachyman according to the weight ratio of 5:3: 14.
The auxiliary protective agent consists of resveratrol and medium-chain triglyceride in a weight ratio of 10: 3.
The filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to the weight ratio of 2:14: 5.
The preparation method of the probiotic composition for losing weight and reducing fat comprises the following steps:
s1, mixing the composite probiotic powder and the auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 4 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding the plant active ingredient and erythritol after uniformly stirring, and continuously stirring for 12min to obtain a mixture A;
s2, adding distilled water with the mass 3 times of that of the filler into the filler, and stirring for 2min at the rotating speed of 1000rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 1.5h at the stirring speed of 6000rpm, adding into an extrusion type microcapsule granulator, carrying out extrusion granulation treatment, and finally carrying out freeze drying on the obtained granules to obtain the microcapsule.
In the step S1, the stirring speed was 250rpm, and the temperature during stirring was 27 ℃.
Example 3A probiotic composition for weight and fat reduction
The probiotic composition for losing weight and reducing fat comprises the following components in parts by weight:
30 parts of composite probiotic powder, 25 parts of plant active ingredients, 16 parts of auxiliary protective agent, 30 parts of filler, 6 parts of erythritol:
the composite probiotic powder is prepared from bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans and lactobacillus acidophilus according to a weight ratio of 2: 1: 1:1, preparing a composition; the viable count of each probiotic is more than or equal to 1 × 109 CFU/g.
The plant active ingredients comprise chlorogenic acid, bamboo leaf flavone and pachyman according to the weight ratio of 7:1: 11.
The auxiliary protective agent consists of resveratrol and medium-chain triglyceride according to the weight ratio of 11: 1.
The filler is composed of galacto-oligosaccharide, hydroxyethyl cellulose and algin according to the weight ratio of 3:11: 4.
The preparation method of the probiotic composition for losing weight and reducing fat comprises the following steps:
s1, mixing the composite probiotic powder and the auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 5 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, adding the plant active ingredient and erythritol after uniformly stirring, and continuously stirring for 15min to obtain a mixture A;
s2, adding distilled water with the mass 4 times of that of the filler into the filler, and stirring for 3min at the rotating speed of 1200rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 2 hours at the stirring speed of 8000rpm, adding into an extrusion type microcapsule granulator, carrying out extrusion granulation treatment, and finally carrying out freeze drying on the obtained granules to obtain the microcapsule.
In the step S1, the stirring speed was 300rpm, and the temperature during stirring was 30 ℃.
Comparative example 1A probiotic composition for weight and fat reduction
Compared with example 2, comparative example 1 is different in that pachyman in the plant active ingredient is replaced with grifola, and other components and preparation steps are the same as those of example 2.
Comparative example 2 probiotic composition for weight and fat reduction
Compared with example 2, comparative example 2 is different in that bamboo-leaf flavone in the plant active ingredient is replaced with ginkgo flavone, and other components and preparation steps are the same as example 2.
Comparative example 3A probiotic composition for weight and fat reduction
Comparative example 3 is different from that of example 2 in that the secondary protective agent is replaced with D-erythorbic acid, and other components and preparation steps are the same as those of example 2.
Comparative example 4A probiotic composition for weight and fat reduction
Compared with example 2, comparative example 4 is different in that medium chain triglyceride in the auxiliary protecting agent is replaced with linoleic acid, and other components and preparation steps are the same as those of example 2.
Comparative example 5A probiotic composition for weight and fat reduction
Comparative example 5 is different from that of example 2 in that galacto-oligosaccharide in the bulking agent was replaced with malto-oligosaccharide, and other components and preparation steps were the same as those of example 2.
Comparative example 6A probiotic composition for weight and fat reduction
In comparison with example 2, comparative example 6 is different in that algin in the filler is replaced with guar gum, and other components and preparation steps are the same as example 2.
Test example I, evaluation experiment of weight-reducing function
1. Test materials: the probiotic compositions prepared in examples 1-3 and comparative examples 1-6.
2. The test method comprises the following steps:
(1) establishing a rat obesity model: the clean SD rat male adult rat has the body weight of 230-290 g and 110 rats and is free to eat and drink water. Animals are fed in a single cage, ordinary feed is fed for observation for 5 days, the animals are randomly divided into 2 groups according to the weight of the rats after the adaptation period is finished, 10 rats are fed with the ordinary feed for maintenance, the rats are used as a blank control group, and 100 rats are fed with high-calorie feed for use as a model group. Recording food intake, food intake and food remaining amount every week, weighing body weight for 1 time, and after continuously feeding for 2 weeks, completing the establishment of a rat obesity model; the high-calorie model feed is prepared according to the requirements in the national food and drug administration & preservation No. 2012/107 annex 8 weight-reducing function evaluation method.
(2) Administering to the subject: the rats of the model group are divided into an example 1-3 group, a comparative example 1-6 group and a model control group, each group comprises 10 animals, the probiotic composition prepared in the example 1-3 group and the comparative example 1-6 group is added into a high-calorie model feed in the test group, the addition amount is 3%, and the model control group is only fed with the high-calorie model feed. The subjects were administered for 6 weeks. The food intake and food remaining were recorded every week, and the body weight was weighed 1 time.
(3) Index detection and recording: after the rats had been given the last time and had an empty stomach for 16 hours, the rats were weighed, anesthetized with 1% sodium pentobarbital [0.5mL/(100 g.bw) ], perirenal fat, peritesticular fat were dissected and weighed, and the fat/body ratio was calculated. Additionally, rats were counted for weight gain, body fat weight (testicular and perirenal fat pads), and fat/body weight.
3. Test results
The test results are shown in table 1.
TABLE 1 Effect of the invention on rat body weight and fat weight
Group of Average initial weight (g) Average final weight (g) Average weight gain (g) Body fat weight (g) Fat/body ratio (%)
Blank control group 354.1 469.4 115.3 11.3 2.41%
Model control group 383.1 558.7 175.6 24.7 4.42%
EXAMPLE 1 group 381.7 509.0 127.3 13.8 2.71%
EXAMPLE 2 group 381.0 505.2 124.2 13.1 2.59%
EXAMPLE 3 group 381.1 507.8 126.7 14.2 2.80%
Comparative example 1 group 381.4 528.9 147.5 16.8 3.18%
Comparative example 2 group 382.6 537.7 155.1 17.7 3.29%
Comparative example 3 group 380.2 517.4 137.2 15.9 3.07%
Comparative example 4 group 382.8 515.3 132.5 15.1 2.93%
Comparative example 5 group 381.5 514.9 133.4 14.8 2.87%
Comparative example 6 group 384.5 534.2 149.7 15.7 2.94%
As can be seen from table 1, after feeding the probiotic compositions prepared in the groups of examples 1 to 3 of the present invention for 6 weeks, the average end weight, weight gain, and body fat weight were significantly reduced compared to the model control group, wherein the group of example 2 had the best effect, and the fat/body ratio was only 2.59%, which is the best example of the present invention. While the average weight gain, in vivo fat weight and fat/body of the obese mice of the comparative examples 1-6 were significantly increased.
Test example II Artificial gastric juice resistance test
1. Test materials: the probiotic composition for losing weight and reducing fat is prepared in the examples 1-3 and the comparative examples 1-6.
2. The test method comprises the following steps: respectively putting 0.2g of the probiotic composition prepared in the embodiments 1-3 and the comparative examples 1-6 in 10mL of artificial simulated gastric juice, shaking and uniformly mixing, incubating in water bath at 37 ℃, sampling every 30min, putting in 0.06mol/L sodium citrate solution, shaking for 1h (35 ℃, 100 rpm) to fully dissolve the composition, counting viable bacteria by adopting a plate counting method, and calculating the survival rate.
The formula of the artificial simulated gastric fluid is as follows: 2g of sodium chloride and 3.2g of pepsin are dissolved in 7ml of 1mol/L HCl solution, water is added to 1000ml, and the pH value is measured to be about 2.0 after preparation.
3. Test results
The survival rates of the probiotics in the simulated gastric fluid in the compositions prepared in examples 1-3 of the present invention and comparative examples 1-6 are shown in table 2.
TABLE 2
Figure 249645DEST_PATH_IMAGE001
As can be seen from table 2, in the case of simulated gastric acid, the probiotic compositions prepared in embodiments 1 to 3 of the present invention show good gastric environment tolerance, and after 2 hours in simulated gastric fluid, the survival rate of the probiotic can still reach more than 89%, wherein the best effect of embodiment 2 is the best embodiment of the present invention. In the comparative examples 3-6, when the components of the auxiliary protective agent and the filler are changed, the survival rate of the probiotics in the obtained composition in simulated gastric juice is reduced to different degrees. The components and the preparation method of the invention can be used for preparing the probiotic composition, which can strengthen the acid resistance of the probiotic composition and protect the probiotic from being damaged in the stomach digestion process.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims (10)

1. The probiotic composition for losing weight and reducing fat is characterized by comprising the following components in parts by weight:
10-30 parts of composite probiotic powder, 15-25 parts of plant active ingredients, 8-16 parts of auxiliary protective agents, 20-30 parts of fillers and 1-6 parts of sweetening agents;
the composite probiotic powder is a combination of two or more of bifidobacterium lactis, lactobacillus rhamnosus, bacillus coagulans, lactobacillus casei, lactobacillus acidophilus and lactobacillus bulgaricus;
the plant active ingredients are two or more of chlorogenic acid, bamboo leaf flavone and pachyman.
2. The probiotic composition for weight loss and fat reduction according to claim 1, which comprises the following components in parts by weight: 25 parts of composite probiotic powder, 20 parts of plant active ingredients, 12 parts of auxiliary protective agent, 23 parts of filler and 4 parts of sweetening agent.
3. The probiotic composition for losing weight and reducing fat according to claim 1 or 2, wherein the auxiliary protective agent consists of resveratrol and medium-chain triglyceride in a weight ratio of 7-11: 1-4.
4. The probiotic composition for weight loss and fat reduction according to claim 3, wherein the co-protectant consists of resveratrol and medium chain triglycerides in a weight ratio of 10: 3.
5. The probiotic composition for losing weight and reducing fat according to claim 1 or 2, wherein the filler is galactooligosaccharide, hydroxyethyl cellulose and algin in a weight ratio of 1-3: 11-17: 4-7.
6. The probiotic composition for losing weight and reducing fat according to claim 5, wherein the filler is galactooligosaccharide, hydroxyethyl cellulose and algin in a weight ratio of 2:14: 5.
7. The probiotic composition for weight loss and fat reduction according to claim 1 or 2, characterized in that the sweetener is erythritol.
8. A method for preparing the probiotic composition for weight loss and fat reduction according to any one of claims 1 to 7, comprising the following steps:
s1, mixing the composite probiotic powder and the auxiliary protective agent, then adding distilled water, wherein the adding amount of the distilled water is 3-5 times of the total mass of the mixture of the composite probiotic powder and the auxiliary protective agent, uniformly stirring, adding the plant active ingredient and the sweetening agent, and continuously stirring for 10-15 min to obtain a mixture A;
s2, adding distilled water with the mass 2-4 times that of the filler into the filler, and stirring for 1-3 min at the rotating speed of 800-1200 rpm to obtain a mixture B;
and S3, adding the mixture A into the mixture B, stirring for 1-2 hours at the stirring speed of 4000-8000 rpm, adding into an extrusion type microcapsule granulator, carrying out extrusion granulation treatment, and finally carrying out freeze drying on the obtained granules to obtain the microcapsule.
9. The method for preparing the probiotic composition for losing weight and reducing fat according to claim 8, wherein the stirring speed in the step S1 is 200-300 rpm, and the stirring temperature is 25-30 ℃.
10. Use of a probiotic composition according to any one of claims 1 to 7 in the preparation of a medicament or health product having a weight-loss effect.
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