KR100846356B1 - Antifungal compositions comprising an extract of enteromorpha sp - Google Patents

Abstract

Compositions comprising the extract of Enteromorpha sp. are provided to inhibit growth of fungi, especially Malassezia sp. causing dandruff without side effects and tolerance, so that the compositions are useful for improvement, prevention and treatment of dandruff and seborrheic dermatitis. An antifungal composition comprises the extract of Enteromorpha sp. selected from Enteromorpha linza, Enteromorpha intestinalis, Enteromorpha compressa, Enteromorpha prolifera and Enteromorpha clathrata, which is prepared by extracting Enteromorpha sp. with a solvent selected from ether, chloroform, benzene, hexane and dichloromethane, wherein the composition shows antifungal effects on Malassezia sp. selected from Malassezia furfur, Malassezia restricta, Malassezia obtusa, Malassezia globosa, Malassezia slooffiae, Malassezia sympodialis and Malassezia pachydermatis. Further, the antifungal composition is used as a composition for preventing or treating dandruff and dermatitis.

Description

ANTIFUNGAL COMPOSITIONS COMPRISING AN EXTRACT OF ENTEROMORPHA SP.}

The present invention relates to an antimicrobial composition against Malassezia sp. Fungi comprising Enteromorpha sp. As an active ingredient.

Dermatitis is an inflammation of the skin and is usually used in the same sense as eczema. It is classified into atopic dermatitis, contact dermatitis and seborrhoeic dermatitis. The atopic dermatitis is an eczema-like skin lesion that occurs in atopic dermatitis, also referred to as endogenous eczema or Benie yangjin. The contact dermatitis refers to inflammation of the skin caused by contact with an external substance, characterized by the so-called skin itching. In addition, the seborrheic dermatitis refers to dermatitis, which occurs well in areas with high secretion of sebum, such as the head, forehead or armpit, and is also called seborrheic eczema. It is a dermatitis mainly caused by erythema and slenderness, or dandruff, and the lightest type is dandruff or scaly tetter.

The dandruff occurs when a problem occurs in the general cycle of skin cells, also known as dry seborrhea, a slight form of seborrheic dermatitis in the scalp, mainly after puberty in which sebaceous glands are actively active, but immediately after birth. Can occur in both sexes.

Recently, it is known that the causative agent of dandruff is a fungus, that is, a yeast bacterium. Especially, it is caused by Malassezia sp. It is reported that if the Malassezia fungi overgrows due to environmental factors such as food and food, dandruff symptoms such as sewage and itching appear, and if the hyperplasia is severe, seborrheic dermatitis occurs (Bergbrant IM Seborrhoeic dermatitis and Pityrosporum yeasts.Current Topics in Medical Mycology. 6: 95-112, 1995). In addition, if a lot of dandruff occurs due to itching, such as excessive scrubbing of the scalp, resulting in wounds, or in some cases may cause inflammation, may cause hair loss.

Recent studies have shown that Malassezia furfur , previously known as the causative agent of dandruff, is associated with seborrheic dermatitis, which occurs mainly in the trunk, and is rarely present in the scalp. Malassezia restrica and Malassezia globosa have been identified as major causative agents of dandruff (Christina M. Gemmer, Thomas L. Dawson Jr, et al., Journal of Clinical Microbiology , Sept. Vol. 40, No. 9, p3350-3357, 2002).

According to the results of the study, it can be said that containing a component that can inhibit the growth of Malassezia sp. Fungi, the main cause of dandruff, has the effect of treating, preventing or improving dandruff and seborrheic dermatitis. Currently, commercially available therapeutic compositions for dandruff and seborrheic dermatitis include antifungal agents ketoconazole, zinc pyrithion, itraconazole or pyroctone olamine. In addition, tar, sulfur, salicylic acid, and the like have been used to alleviate seborrheic dermatitis and phosphorus, and selenium sulfide has been used to suppress the hyperproliferation of epidermal cells. However, the antifungal agents that have been used for the treatment of dandruff have a good effect on the treatment of dandruff, while most of them are artificially synthesized, which raises questions about human safety.

For example, long-term use of ketoconazole, an active ingredient of an anti-dandruff treatment such as nizoral, which is commercially available in Korea, poses a problem that may cause adverse effects on the endocrine system (Schuermeyer T, Nieschlag E. 1982. Ketoconazole induced drop in serum and saliva testosterone.Lancet 1098p; Janssen PAJ, Symoens JE. 1983. American Journal of Medicine 74: 80-85). In addition, zinc pyrithione exhibits excellent effects as an antifungal agent for inhibiting scalp hyperkeratosis, but is insoluble in water and has a high specific gravity, which makes it difficult to spray uniformly in the composition. Gene A. Hyde and Mark M. Auerbach, Formulation techniques for zinc pyrithione antidandruff shampoos.Cosmetics and Toiletries, 94, 57, April, 1979; Fred H. Snyder et al , Safety evaluation of Zinc 2-Pyridiethiol 1 -Oxide in Shampoo Formulation, Toxicology and Applied Pharmacology, vol. 7, p.425-437, 1965; Marks, R. The effects of a shampoo containing zinc pyrithione on the control of dandruff.Br J. Dermatol. 112. 415, 1985). In particular, zinc pyrithione causes skin dermatitis (Nielsen, NH, Allergic contact dermatitis caused by zinepyrithione associated with pustular psoriasis. Am J. Contact Dermat. 8, 170. 1997), which may cause problems such as environmental toxicity. Because of this, it is difficult to apply to hair cosmetics at high concentration.

In addition, itraconazole, which has been studied to replace ketoconazole, is highly effective against many pathogenic fungi related to humans and has a high affinity for keratinous tissues (Van Cutsem J, Van Gerven F, Zaman R, Heeres J, Hanssen PAJ.Pharmacological and preclinical results with a new oral and topical broad spectrum antifungal R 51211. Proceedings of the 13 ^th International Congress of Chemotherapy, Vienna, Part 40: 1-11, 1983), such as ketoconazole because they are not transported through the sweat glands and the disadvantage of the oral cavity is somewhat slower transport during the zero dose, in order to prevent a satisfactory therapeutic effect and recurrence has been reported to require the prescription of a minimum daily 1,000 mg (Grant SM, Clissold SP . Drugs 37: 310-344, 1989). In addition, pyroctonolamine and salicylic acid, which are mainly used as bactericides and keratin solubilizers, were studied to make up for the shortcomings of zinc pyrithione.

In addition, the majority of patients are prescribed several kinds of medicines at the same time, but the administration of a combination of drugs can affect the liver cells toxicity and immune system, and may cause problems that can increase the efficiency or adverse effects of a drug (Svejgaard E. Side effects and interactions of oral antifungals. Journal of the European Academy of Dermatology and Venereology 5, page S11, 1995; Bickers DR. Journal of the American Academy of Dermatology 31: S87-S90, 1994) It also causes symptoms such as nausea (Georgiou S, Monastirli A, Pasmatzi E, Zografakis C, Tsambaos D. Oral itraconazole therapy for pityriasis versicolor.Journal of the European Academy of Dermatology and Venereology 8: 203-207, 1997).

Dandruff is not a serious disease that shortens human life, but because it causes psychological stress in the majority of people, methods or ingredients to prevent or treat such dandruff are constantly being studied. Harmless to the environment and humans, research on the ingredients that can cause a reduction in the number of dandruff is still incomplete.

Therefore, unlike the existing antifungal substances, there is a growing demand for the development, prevention, and treatment of anti-dandruff derived from natural substances, which have no side effects and long-term intake or use, without problems of resistance or stability. Especially, considering the geographical environment of Korea, which is rich in marine resources, it has excellent antibacterial activity against Malassezia sp. Fungi, the causative agent of dandruff, from marine natural materials including marine animals and plants. There is an increasing demand to develop compositions.

Therefore, the technical problem to be achieved by the present invention is to solve the problems of the prior art, the genus Malassezia ( caused as a causative agent of dandruff and / or seborrheic dermatitis including Enteromorpha sp. Extract as an active ingredient) Malassezia sp.) It provides an antimicrobial composition against fungi and at least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition as an active ingredient.

In order to achieve the above object, the present invention is a genus Enteromorpha sp.) provides an antimicrobial composition against Malassezia sp. fungi, including the extract as an active ingredient.

In addition, the present invention provides a composition for the prevention or treatment of dandruff and / or seborrheic dermatitis comprising the antimicrobial composition as an active ingredient.

The present invention also provides a cleaning composition for preventing or improving dandruff and / or seborrheic dermatitis, comprising the antimicrobial composition as an active ingredient. The detergent composition may be applied to the hair and / or scalp or to the skin.

The present invention also provides a cosmetic composition for the prevention or improvement of dandruff and / or seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The cosmetic composition may be applied to the hair and / or scalp or to the skin.

Hereinafter, the present invention will be described in detail.

In the present invention, the palette in (Enteromorpha sp.) Plant is a plant belonging to Chlorophyta ulvaceae leaf palette (Enteromorpha. Linza), bowel palette (Enteromorpha. Intestinalis), flat laver (Enteromorpha. Compressa), visible green laver (Enteromorpha prolifera ) and lattice green ( Enteromorpha. clathrata ) may be any one selected from the group consisting of, and may be a leaf green. Most of the genus plants have a cylindrical shape of the frond is branched or forged, depending on the type, the upper part can spread to the leaf, and taxonomy includes hollow one kind of cylindrical shape. They grow mainly in the upper tidal flats of the seashore, especially where fresh water flows, and often form large colonies in quiet tidal pools. It breeds from late autumn to early summer and is used for food in Korea and Japan.

The leaf algae ( Enteromorpha linza ) is a green algae belonging to the sea horse of the green algae, the leaf is an elongated and flat frond, forging, and the lower part may shed some branches. It is widely distributed all over the world, and in Korea, it is distributed all over the coast, and mainly lives on rocks in the upper intertidal zone or woodwork in the bay. In addition, leaf greens are mainly used for food and may be used as kimchi or soup.

The leaf lanceolate is very thin in the lower part of the body, wrinkled or flat at the edge, 10 to 20 cm in length, 5 to 10 cm in width, greenish green, and frond is pale membranous.

The extraction solvent of the present invention may be any one selected from the group consisting of nonpolar solvents of ether, chloroform, benzene, hexane or dichloromethane and mixed solvents thereof, preferably consisting of ether, chloroform, benzene, hexane and dichloromethane. It may be any one selected from the group, more preferably ether. In particular, in the case of leaf green ether extract, antifungal activity against Malassezia fungi, the causative fungus of dandruff, was found to be remarkably excellent (Table 1). On the other hand, in the case of extract extracted with a polar solvent such as water or methanol, antifungal activity against Malassezia fungi was not observed (Table 1).

Leaf seaweed extract of the present invention may be prepared according to a conventional method for preparing seaweed extract, specifically may be cold extraction, hot extraction or heat extraction, etc., may be used a conventional extraction device, ultrasonic grinding extractor or fractionator have.

In addition, the extract extracted with the solvent may be further subjected to the fractionation process with a solvent selected from the group consisting of a non-polar solvent of ether, chloroform, benzene, hexane, methylene chloride or dichloromethane and a mixed solvent thereof. The fractionation process may be performed at 4 ℃ to 120 ℃, but is not limited thereto. In addition, one or more solvents may be used in the fractionation.

The prepared extract or the fraction obtained by performing the fractionation process can be filtered or concentrated or dried to remove the solvent, it can be carried out both filtration, concentration and drying. Specifically, the filtration may be performed using a filter paper or a reduced pressure filter, the concentration may be concentrated under reduced pressure using a reduced pressure concentrator, for example, a rotary evaporator, and the drying may be performed by, for example, a lyophilization method.

In one embodiment of the present invention, the foliar extract may be obtained by the process shown in FIG. More specifically, after removing the salt and impurities of the leaves and dried, to prepare a dried sample, by adding ether to the dried sample and extracted at room temperature for 4 hours to 36 hours, preferably 12 hours to 24 hours Obtaining an extract, the crude extract may be concentrated under reduced pressure to prepare an extract. At this time, the removal of the salt and impurities of the lamella may be carried out by washing with running water, and the dry sample may be prepared by drying after crushing the lamellae from which the salt and impurities are removed. In addition, the obtained green leaf extract can be stored in a deep freezer until use.

In addition, the leaf green leaf extract may be one obtained by completely removing the water by concentrating and lyophilizing the obtained extract, the leaf green leaf extract is completely removed the water is used in powder form or the powder is ether or conventional non-polar It can be dissolved in a solvent and used.

The present invention provides an antimicrobial composition against Malassezia fungi comprising leaf greens extract as an active ingredient. The green leaf extract may be a crude extract obtained by the extraction solvent or a fraction obtained by the fractional solvent.

In the present invention, "antimicrobial composition" may mean the same as an antibiotic, which is a generic term for an antimicrobial agent, may have the same meaning as an antifungal agent, fungicide, preservative, preservative or fungicide, preferably seborrheic dermatitis and / or By pathogenic microorganisms, including Malassezia sp. Fungi, which cause skin diseases such as dandruff, it means substances that can inhibit or inhibit the development and life functions of fungi.

In the present invention, "malusian fungus" means all fungi belonging to the genus Malassezia , for example, the genus Malassezia fungus is Malassezia furfur , Malassezia restricta , Malassezia restricta , Do three Zia of the projection (Malassezia obtusa), dry aged Jia global Bossa (Malassezia globosa), dry aged Jia slow Opie kids (Malassezia slooffiae), dry aged Jia Symposium Diallo lease (Malassezia sympodialis) and do three Zia Parkinson more Mathis (Malassezia pachydermatis ) may be at least one selected from the group consisting of, preferably at least one selected from the group consisting of Malaysian restricta , Malesesia globosa , Malesesia puffer and Maleseviia obtusa.

The antimicrobial composition of the present invention may contain 0.001 to 99.99% by weight, preferably 0.1 to 99% by weight, based on the total weight of the composition, the active ingredient according to the method of use and purpose of use of the antimicrobial composition. The content of can be adjusted appropriately.

The antimicrobial composition of the present invention may further include sugars, proteins, lipids, vitamins and minerals, etc., in addition to the green leaf extract. The sugars, proteins, lipids, vitamins or minerals may be appropriately selected according to the purpose and use thereof. For example, the sugars may be honey, dextrin, sucrose, palatinose, glucose, fructose, syrup, and sugar alcohol. ), Sorbitol, xylitol and maltitol, the protein may be milk-derived proteins such as casein, whey protein, soy protein, trypsin of these proteins, and enzymes derived from pepsin and neutra It may be a hydrolyzate by neutrase, alkilase, the lipid is a monohydric saturated fatty acid, sunflower oil containing polyunsaturated fatty acid, rapeseed oil, olive oil, safflower oil , Corn oil, soybean oil, palm oil, palm oil, and other plant-derived oils such as palm oil, middle-chain fatty acids, EPA, DHA, soybean-derived phospholipids and milk-derived phospholipids, The minerals may be potassium phosphate, potassium carbonate, potassium chloride, sodium chloride, calcium lactate, calcium gluconate, calcium pantothenate, calcium casein, magnesium chloride, ferrous sulfate, and sodium bicarbonate, but are not particularly limited by the examples. no.

For any of the above saccharides, proteins, lipids, vitamins and minerals, as long as the antimicrobial properties of the composition are maintained, it is not limited to the above embodiments and may further include other ingredients, and each content is limited so long as the antimicrobial properties are maintained. Not preferably, it may be 0.1 to 15% by weight, preferably 0.5 to 10% by weight relative to the total composition.

In addition, the antimicrobial composition is an additive of various cosmetic compositions and dermatology for the purpose of sterilization of Malassezia fungi in addition to the active ingredient in a range that does not maintain the effects of the present invention, and does not cause side effects due to the added ingredients. Preferably, it is selected from the group consisting of ketoconazole, zinc pyrithion, itraconazole, pyroctone olamine, selenium sulfide, tar, sulfur and salicylic acid, which are conventional anti-dandruff components (ketoconazole), zinc pyrithion (zinc pyrithion) It may further include any one, the additional component or additive may be provided in a range that does not interfere with the composition of the antimicrobial composition.

The antimicrobial composition of the present invention can be used for various purposes and uses that require antimicrobial activity, specifically, can be used for the inhibition of the growth of pathogenic microorganisms, for example pathogenic fungi, dandruff or dermatitis caused by pathogenic fungi, Preferably it can be used for the purpose of disinfection of the genus Malassezia or for the treatment, prevention or improvement of dandruff or seborrheic dermatitis caused by the Malassezia fungi.

More specifically, since the antimicrobial composition of the present invention exhibits antimicrobial activity against the male bacterium restritta, which is the causative agent of dandruff, among the Malassezia fungi (Figure 2), the treatment, improvement or prevention of dandruff It can be applied to a cleaning composition or cosmetic composition which can be applied to at least one selected from the group consisting of a composition for preventing or treating dandruff or a hair and a scalp having an excellent effect. In addition, since it exhibits antimicrobial activity against the male fungus puffer, which is the causative agent of seborrheic dermatitis mainly affecting the trunk (FIG. 3), the composition for preventing or treating seborrheic dermatitis or improving or preventing seborrheic dermatitis. It is applicable to the detergent composition or cosmetic composition which can be applied.

In addition, the present invention provides a composition for the prevention or treatment of at least one, preferably dandruff, selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The leaf green leaf extract of the present invention has antifungal activity against the fungus of Malassezia fungi, more specifically maleesia restricta, which is the causative agent of dandruff, and malesperia puffer, which is the causative agent of seborrheic dermatitis. The antimicrobial composition has a prophylactic and / or therapeutic effect on at least one selected from the group consisting of dandruff and seborrheic dermatitis, preferably dandruff.

In the present invention, dandruff is the skin of the head, ie, the stratum corneum cells of the scalp, is simply inflamed away and appears as a dry or wet or oily scale. Many cases of dandruff are referred to as dandruff, medically referred to as dry seborrhea. When the dry seborrhea is worsened, it may be a dermatitis seborrheica, and the seborrheic dermatitis may be accompanied by deprivation and itching of a large amount of dry scales. In addition, alopecia which occurs when the dandruff caused by dry seborrhea is chronically severe is called alopecia seborrheica. In the case of dandruff or seborrheic dermatitis, the main causative agent is reported as the genus Malassezia fungi. Therefore, in order to treat the dandruff or seborrheic dermatitis, it is possible to prescribe a substance having antimicrobial activity against the causative fungus Malassezia.

At least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis of the present invention can be applied directly to animals including humans. The animal is a biological group corresponding to plants, and mainly ingests organic matter as nutrients, and means that digestion, excretion, and respiratory organs are differentiated, preferably mammals, and more preferably humans.

At least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis may include the antimicrobial composition alone as an active ingredient, and additional ingredients, ie, pharmaceuticals, depending on the formulation, method of use, and purpose of use. Or further nutritionally acceptable carriers, excipients, diluents or accessory ingredients.

More specifically, at least one composition for prevention or treatment selected from the group consisting of dandruff and seborrheic dermatitis, in addition to the active ingredient in addition to nutrients, vitamins, electrolytes, flavors, coloring agents, neutralizing agents, pectic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like may further be contained. In addition, the carrier, excipient or diluent may be lactose, dextrose, sucrose, sorbitol, mannitol, ziitol, erythritol, maltitol, starch, acycia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline From the group consisting of cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, dextrin, calcium carbonate, propylene glycol, liquid paraffin, physiological saline It may be one or more selected, but is not limited to, any conventional carrier, excipient or diluent can be used. The components may be added independently or in combination to the antimicrobial composition that is the active ingredient.

At least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis is 0.001% to 99.9% by weight of the antimicrobial composition, preferably 0.1% to 99% by weight, more preferably, based on the total weight of the composition. May include 1% to 50% by weight. In addition, the content of the additional component may be preferably 0.1 to 20 parts by weight based on 100 parts by weight of the antifungal composition.

In addition, at least one composition for prevention or treatment selected from the group consisting of dandruff and seborrheic dermatitis, when formulated, conventional fillers, extenders, binders, disintegrants, surfactants, anticoagulants, lubricants, wetting agents, fragrances, emulsifiers Or may further include preservatives, or both oral or parenteral.

Specifically, solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, calcium carbonate, It is prepared by mixing sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, solvents, emulsions, and syrups.In addition to the commonly used simple diluents, water and liquid paraffin, various excipients may include, for example, wetting agents, sweeteners, fragrances, and preservatives. have.

Forms for parenteral administration include topical administrations such as creams, ointments, dressing solutions, sprays, and other coatings. As an example of the formulation of the topical dosage form, an antifungal composition or an anti-fungal composition selected from the group consisting of dandruff and seborrheic dermatitis is impregnated into a carrier such as gauze made of natural or synthetic fibers. Can be. When formulated as a parenteral preparation in the form for parenteral administration, it may be prepared using conventional fillers, extenders, binders, wetting agents, disintegrating agents, excipients and the like. Preparations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerol or gelatin may be used.

In the case of the cream or ointment, it may be suitable for direct application to or around the affected area of one or more diseases selected from the group consisting of dandruff and seborrheic dermatitis. In the case of the spraying agent, it can be prepared by a conventional spraying method, except that it contains an antimicrobial composition as an active ingredient, and is packed in a compression container or other spraying container and sprayed on the dandruff site from time to time to prevent dandruff or seborrheic Dermatitis can be prevented or treated.

In addition, the formulation of the one or more prophylactic or therapeutic compositions selected from the group consisting of dandruff and seborrheic dermatitis of the present invention may be in a preferred form depending on the method of use, in particular the rapid, sustained or delayed action of the active ingredient after administration to a mammal. Methods known in the art can be employed and formulated to provide release. Examples of specific formulations include warnings, granules, lotions, liniments, limonades, fragrances, powders, syrups, ointments, liquids, aerosols and extracts. , Elixier, ointment, liquid extract, emulsion, suspension, premises, acupuncture, eye drops, tablets, suppositories, injections, spirits, cataplasma, capsules, creams, pills, troches ), Tincture, pasta, soft or hard gelatin capsules.

Furthermore, one or more compositions for the prophylaxis or treatment selected from the group consisting of dandruff and seborrheic dermatitis of the present invention can be prepared using any suitable method known in the art or by Remington's Pharmaceutical Science (Recent Edition), Mack Publishing Company. , Easton PA).

The dosage of the at least one prophylactic or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis according to the present invention is appropriately determined by those skilled in the art in consideration of the method of administration, the age, sex and weight of the recipient, and the severity of the disease. Can be selected. For example, at least one prophylactic or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis of the present invention is 0.0001 mg / kg to 1000 mg / kg, based on the antimicrobial composition, to be more effective 0.01 mg / kg to 100 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the composition for preventing or treating at least one selected from the group consisting of dandruff and seborrheic dermatitis of the present invention may further comprise a compound or plant extract having an inhibitory activity of known dandruff or seborrheic dermatitis in addition to the antimicrobial composition. 5 parts by weight to 20 parts by weight, based on 100 parts by weight of the antimicrobial composition, may be included.

The present invention also provides a cleaning composition for preventing or improving at least one selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The detergent composition may be applied to the hair and / or scalp or to the skin.

The present invention also provides a cosmetic composition for preventing or improving at least one selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition as an active ingredient. The detergent composition may be applied to the hair and / or scalp or to the skin.

In the present invention, hair refers to human hair as a generic term for body hair and hair, preferably hair, and may include hair roots of hair, hair follicles, hair and scalp near hair follicles. In the present invention, the scalp refers to the skin covering the head area, usually the hair (hair) area.

The detergent composition may be applied to one or more selected from the group consisting of hair and scalp, or may be applied to the skin. When applied to the skin, it is also called a body cleanser composition.

The cleaning composition comprising the antimicrobial composition as an active ingredient is a substance commonly used in the preparation of the active ingredient in addition to the active ingredient, for example, a surfactant, a tax aid, a pH adjuster, a moisturizing agent such as a natural moisturizing ingredient and a natural vegetable oil, and an antioxidant. , Metal ion sequestrants, preservatives, solvents, flavoring agents and pigments may be further included. In addition, the three tablet composition may further include an additional antimicrobial substance or fungicide within the range of maintaining the antimicrobial effect, and does not irritate the human body.

The "cleaning agent composition" is a composition used to remove oily and aqueous pollution or wastes attached to the skin surface with moderate foaming power in cold water and hot water. It provides additional functions such as moisturizing effect, increase skin flexibility, and increase sebum removal ability. In addition, recently, the skin hypoallergenicity of the cleaning composition is emphasized.

In general, inflammation of human skin is caused by bacteria in the sebum in the pores, or caused by allergic factors. When washing with the cleaning composition, the skin condition is obtained by obtaining skin sebum removal and sterilization effects depending on the ingredients contained therein. Can be improved. In addition, the role of removing the waste or sebum in the cleaning composition is mainly a surfactant, and bacterial infection prevention is performed by a fungicide or an antimicrobial agent, since the currently used fungicide or antimicrobial material is mainly a chemical synthetic material, a chemical synthetic material When used on sensitive skin or excessively used to a certain level or more, there is a problem that can cause skin damage by contact with the skin.

Therefore, efforts to replace the fungicides or antimicrobial substances with skin hypoallergenic or harmless natural substances continue, and in this respect, the green leaf extract, which is an antimicrobial component of the antimicrobial composition as an active ingredient of the cleaning composition of the present invention, is an edible natural plant. As a hypoallergenic skin.

In addition, the antimicrobial composition has excellent antimicrobial activity against Malassezia fungi, particularly male pulp, the causative agent of seborrheic dermatitis mainly in the trunk, and male restagria, a causative agent of dandruff. Therefore, the cleaning composition of the present invention has a remarkable and excellent effect in that it has a cleaning and moisturizing effect which is a basic function of the cleaning composition, is hypoallergenic, and can improve or prevent one or more selected from the group consisting of dandruff and seborrheic dermatitis. Is admitted.

The surfactant may be any one selected from the group consisting of anionic surfactants, nonionic surfactants, cationic surfactants and amphoteric surfactants.

The anionic surfactant may be, for example, ammonium lauryl sulfosuccinate, ammonium lauryl sulfate, sodium cocoyl isionate, sodium lauryl isceionate, sodium lauryl sulfate, triethanolamine lauryl sulfate or sodium lauryl ether sulfate And the like. The nonionic surfactant may be, for example, polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene, polysorbate 80, hardened castor oil derivative, fatty acid diethanolamide, glyceryl stearate, or the like. The cationic surfactant may be, for example, a tertiary aliphatic amine salt, alkyltrimethylammonium chloride, or dialkal methylammonium chloride. The amphoteric surfactant may be, for example, betaine, amidebetaine type, sulfobetaine type, or steartrimonium chloride.

The pH adjusting agent may be, for example, organic acids such as citric acid, succinic acid, tartaric acid, lactic acid, malic acid, fumaric acid, maleic acid, glycolic acid, hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, and the like, or a sodium salt, potassium salt, or ammonium salt thereof. Triethanolamine, sodium chloride, sodium hydroxide or potassium hydroxide, and the like.

The moisturizing agent is a conventional oil used for moisturizing, for example, vegetable oil or liquid paraffin, ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, sorbitol, Collagen and the like. The moisturizing agent may be contained in an amount of 0.1 to 10 parts by weight based on the total weight of the composition, but is not limited thereto.

The preservative may be, for example, benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin, methyl paraben, ethyl paraben, or propyl paraben.

The solvent may be, for example, purified water, ethanol, tween 20, cyclomethicone, mineral oil or dimethicone, and the above-mentioned agents and pigments may be appropriately selected by those skilled in the art. It is possible to use ingredients used in common detergent compositions.

In addition, the detergent composition may be formulated as a liquid, solid soap or powder.

When the cleaning composition is applied to at least one selected from the group consisting of hair and scalp, the cleaning composition is a shampoo, rinse, shampoo and rinse blend, treatment, soap, bar or tablet suitable for cleaning skin, soap bar or Soap tablets and the like.

When the detergent composition is used for shampoo and the like for hair and scalp cleansing, it may further include a surfactant. For example, the surfactant may be any one or more selected from the group consisting of synthetic anionic surfactants, amphoteric surfactants, and nonionic surfactants, and the anionic surfactants may be alkyl sulfates or alkyl ether sulfates, The amphoteric surfactant may be alkylbetaine or alkylamidopropylbetaine, the nonionic surfactant may be alkanolamide or alkylamine oxide, and the surfactant may be used alone or in combination.

In addition, the cleaning composition for hair and scalp cleansing may further include conventional ingredients for maintaining the basic properties and quality of the cleansing use, such as shampoo, such components are pearl foil auxiliary, preservative, thickener, viscosity Regulators, pH regulators, fragrances or dyes and the like.

The pearlescent auxiliary agent may be ethylene glycol monostearate or ethylene glycol distearate, and the preservative may be methyl paraoxybenzoate, methylchloroisothiazolinone, methylisothiazolinone, or a mixture thereof, The agent and the viscosity adjusting agent may be sodium chloride, ammonium chloride or propylene glycol, the pH adjusting agent may be citric acid, phosphoric acid, sodium hydroxide or potassium hydroxide, the dye may be a water-soluble tar and the like. In addition, the hair and scalp cleansing composition may further include a hair conditioning agent such as polyquaternium-10, polyquaternium-7, methylpolysiloxane, dimethylconcopolyol or hydrolyzed animal protein.

The cosmetic composition may be for applying to the hair and / or the scalp or to the skin, for example may be a hair cosmetic composition.

When the cosmetic composition is a hair cosmetic composition, a hair conditioner, hair tonic, hair cream, hair lotion, hair face, hair gel, hair pack, hair massage, hair liquid, hair spray, hair mousse, aerosol mousse, and aerosol spray , Aerosol mousse, aerosol spray, perm, bleach, water dispersion, powder or oil. The composition of each formulation may contain a variety of bases and additives necessary for the formulation of the formulation and appropriate, the type and amount of these components can be easily selected by those skilled in the art within the range that can maintain the effect of the active ingredient. have.

When the formulation of the hair cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silita, talc or zinc oxide may be additionally added. It may include.

When the formulation of the hair cosmetic composition is a powder or a spray, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, and in particular, when the formulation is a spray, additionally chlorofluoro Propellants such as hydroxycarbons, propane, butane or dimethyl ether.

When the formulation of the hair cosmetic composition is a solution or emulsion, it may include a solvent, a solubilizer or an emulsifier, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan, and the like.

When the formulation of the hair cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like may further be included.

Antimicrobial composition comprising the extract of Enteromorpha sp. Of the present invention as an active ingredient has an antimicrobial activity against the Malassezia sp. Fungi, the causative agent of dandruff, for excellent prevention or treatment of dandruff Not only does it have an effect, but unlike conventional antibiotics, it contains extract extracted from leaf seaweed, which is used for food, as an active ingredient, so it does not cause side effects, and it is economical because it uses leaf seaweed, which is rich in algae. It is also advantageous because it is industrially very effective.

Hereinafter, preferred embodiments of the present invention are described. However, the following examples are only preferred embodiments of the present invention, and the present invention is not limited to the following examples.

Example 1: Preparation of Leaf Laver Extract

Leaf greens used for the test were collected in March 2006 in the sea off Busan Cheongsapo, and the collected leaf greens were removed from the deposit, washed with clean fresh water, completely dried at room temperature, and then ground using a grinding machine. Water, methanol, and ether extracts of the pulverized lamella were prepared by the method described in FIG. 1, respectively.

In more detail, as shown in FIG. 1, 300 ml ether or methanol was added to 20 g of the pulverized leaf pulverized powder, and extracted at room temperature for one day to obtain crude extracts. The crude extract was filtered, the filtrate was concentrated under reduced pressure using a vacuum evaporator, sterilization was performed using a 0.22 μm filter, and the concentration of the sterilized extract was adjusted to 40 mg / ml, respectively, ether extract and Methanol extracts were prepared. The obtained ether extract and methanol extract were stored at -20 ° C in a deep freezer until use.

The methanol extract was filtered and 100 ml of methanol and the same amount of distilled water were added to the remaining residue, followed by extraction at room temperature for one day, followed by sterilization using a 0.22 μm filter, and the concentration of the sterilized water extract to 40 mg / ml. Adjusted to prepare a water extract. The obtained water extract was stored at -20 ° C in a deep freezer until use.

The total amount of the ether extract, methanol extract and water extract obtained from 20 g of the leaf green dry pulverized product was 0.38 g, 1.86 g and 0.36 g, respectively.

Example 2: Determination of antifungal activity of green leaf extract

For the crude extracts of the lamellae prepared in Example 1, antifungal activity against Malassezia sp. Fungi, the causative agent of dandruff, was measured using the Agar diffusion method.

2-1: Target strain and culture medium

The Malassezia sp. Fungi strain Malassezia restricta or M. restricta strains (KCTC 7848) and Malassezia furfur ( M. restricta ) strains (KCTC 7743) are Korea Biotechnology Research Institute was used for pre-sale of biological resources center (KCTC), the strain was cultured at a temperature of 34 ℃ using a culture medium having the following composition.

Composition of culture medium

Modified Leeming and Notman agar medium (0.1% polypeptone, 2.0% glucose, 5.0% Malt extract, 2.0% desiccated ox bile, 0.2% glycerol, 1.0% Tween 40 and 2.0% agar in distilled water)

2-2: Antifungal Activity Measurement Using Paper Disk

Antifungal activity against the Malassezia sp. Fungi, the causative agent of dandruff, was measured by plate medium diffusion using a paper disk, and the antifungal activity was prepared in Example 1, which is an antifungal target. The paper disks containing the leaves of each leaf extract were used.

Specifically, 5 mg of the ether, water, or methanol leaf green extract prepared in Example 1 was dissolved in 30 μL of the same solvent as the extraction solvent, respectively, and then a paper disk having a diameter of 8 mm and a thickness of 1.5 mm (Advantec Filter Paper, Toyo Roth Kaisha Ltd, Japan) and absorbed, the solvent was completely evaporated, and then the mLNA (Modified Leeming and Notman agar medium) of each strain of Malassezia fungi cultured in the culture medium prepared in Example 2-1 ) Was placed on a plate medium to measure the antimicrobial activity.

In the case of the control group, the same solvent as the ether, water, or methanol leaf green extract was used in the same amount to perform the absorption and evaporation to determine the antimicrobial activity as described above. The strain was cultured at a temperature of 34 ℃ using the culture medium prepared in Example 2-1, after 48 hours of incubation, the size (in mm) of the circular growth resistant ring formed around the disk was measured. The experiment was repeated three times independently, and the average value of the three experiments was measured and shown in Table 1 below.

As a result of measuring the antifungal activity, as shown in Table 1 and FIG. 2 and FIG. 3, in the case of the leaf green ether extract, both of the Malassezia restricta strain and Malassezia puffer strain had excellent antifungal activity. It was confirmed (Table 1, FIG. 2 and FIG. 3).

On the other hand, as shown in Table 1 and Figures 4 to 7, the leaf green water extract or leaf green methanol extract was found to have no antifungal activity (Table 1, Figures 5 to 7). The numerical value shown in Table 1 is the value of the diameter of the inhibitor ring excluding the size of the diameter of the disk 8mm ((measured value-8mm) / 2).

Ether extract (mg) Methanol Extract (mg) Water extract (mg) Extract content One 3 5 One 3 5 One 3 5 M. restricta Average (mm) 0 16 20 0 0 0 0 0 0 Error (mm) - ± 0.5 ± 0.2 - - - - - - M. furfur Average (mm) 10 16 24 0 0 0 0 0 0 Error (mm) ± 0.4 ± 0.2 ± 0.3 - - - - - -

That is, the paper disk treated with the ether extract prepared in Example 1, compared with the control not treated with the extract, in the case of M. restricta ( M. restricta ) from 3 mg, Malassezia puffer ( M In the case of furfur ) it was confirmed that the antimicrobial activity appeared from 1 mg (Table 1), the antifungal activity was increased in proportion to the concentration of the extract extract (Fig. 2 and 3). On the other hand, methanol extracts and water extracts did not show antifungal activity against each of the fungi at all concentrations of 1 mg, 3 mg and 5 mg (Table 1, FIGS. 4-5, and 6-7).

In summary, the antifungal activity against the leaves of Malassezia sp., The causative agent of dandruff, was determined using the Agar diffusion method. for the extract, and determined that no antifungal activity, in the case of the extract with the apolar solvent is ether, and dry organisms do not count the subgenus of dandruff (Malassezia sp.) fungi which do three Jia less trick other (M. restricta) Concentration-dependent antifungal activity was recognized for all M. furfur , and the green leaf extract was found to be excellent in the treatment, improvement or prevention of dandruff.

In particular, since the foliar is obtained from natural plants used as food materials since ancient times, no problems such as side effects of existing antifungal agents or compounds are generated, and thus foliar is used as a seaweed that can be easily obtained from the coast of Korea. Economically beneficial, the industrial value is very large.

Example 3 Preparation of a Hair Composition Comprising Leaf-Palm Extracts

3-1: Preparation of anti-dandruff shampoo

Anti-dandruff shampoo including the leaf green ether extract prepared in Example 1 was prepared in the following composition.

3 g of green leaf ether extract of Example 1

30 g of 95% ethanol

0.5 g of surfactant 1 (alkylether sulfate)

0.5 g of Surfactant 2 (sodium lauryl ether sulfate, 2.2 EQ)

2.4 g of kosylbetaine

Water g 100 g

3-2: Preparation of anti-dandruff lotion

The anti-dandruff lotion including the leaf green ether extract prepared in Example 1 was prepared in the following composition.

1 g of green leaf ether extract of Example 1

30 g of 95% ethanol

Water gs 100g

3-3: for cleaning Body cleanser  Preparation of the composition

Body cleanser composition comprising a leaf green ether extract prepared in Example 1 was prepared in the following composition.

2 g of green leaf ether extract of Example 1

18 g anionic surfactant

5 g of nonionic surfactant

7 g of glycerin

3 g of sodium chloride

Natural olive liquid soap 1.5 g

1 g of spices

Water gs 100g

1 is a flow chart showing a process for producing an extract of foliar according to an embodiment of the present invention.

FIG. 2 is a paper disk showing antimicrobial activity against Malassezia restricta , a Malassezia sp. Fungi, which is a causative agent of dandruff, according to the amount of the leaf-derived ether extract, according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using. Counterclockwise from top left, with control, 1 mg, 3 mg, and 5 mg of foliar ether extract.

3 is a paper disc using antimicrobial activity against Malassezia furfur , a Malassezia sp. Fungus, Malassezia sp., Which is a causative agent of dandruff, according to the amount of leaf green ether extract according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method. Counterclockwise from top left, with control, 1 mg, 3 mg, and 5 mg of foliar ether extract.

Figure 4 is an antibacterial activity against Malassezia restricta , a Malassezia sp. Fungus Malassezia sp., Which is a causative agent of dandruff, according to the amount of methanol extract of leaf green tea according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using. Counterclockwise from top left, with control, 1 mg, 3 mg, and 5 mg of foliar ether extract.

5 is a paper disk having antimicrobial activity against Malassezia furfur , a Malassezia sp. Fungus Malassezia sp., Which is a causative agent of dandruff, according to the amount of methanol extract of leaf green according to an embodiment of the present invention. It is a photograph measured by the used plate medium diffusion method. Counterclockwise from top left, with control, 1 mg, 3 mg, and 5 mg of foliar ether extract.

FIG. 6 is a paper disk showing the antimicrobial activity against Malassezia restricta , a Malassezia sp. Fungi, which is a causative agent of dandruff, according to the amount of leaf-seed water extract according to an embodiment of the present invention. It is a photograph measured by the plate medium diffusion method using. Counterclockwise from top left, with control, 1 mg, 3 mg, and 5 mg of foliar ether extract.

Figure 7 is a photograph of the antimicrobial activity against the Malassezia furfur ( Malassezia furfur ), which is the causative agent of dandruff according to the amount of the water extract of leaf green tea according to an embodiment of the present invention is measured by a plate medium diffusion method using a paper disk. Counterclockwise from top left, with control, 1 mg, 3 mg, and 5 mg of foliar ether extract.

Claims (8)

Antimicrobial composition against Malassezia sp. Fungi comprising Enteromorpha sp. Extract as an active ingredient. The method of claim 1, wherein the palette in plant leaves palette (Enteromorpha. Linza), bowel palette (Enteromorpha. Intestinalis), flat laver (Enteromorpha. Compressa), visible green laver (Enteromorpha. Prolifera) and grid palette (Enteromorpha. Clathrata) Any one selected from the group consisting of, antimicrobial composition. According to claim 1, The genus Malassezia fungus Malassezia furfur ( Malassezia furfur ), Malassezia resta ( Malassezia restricta ), Malassezia obtusa , Malassezia globosa ), Malassezia slooffiae , Malassezia sympodialis and Malassezia pachydermatis , at least one selected from the group consisting of antimicrobial compositions. The antimicrobial composition of claim 1, wherein the genus extract is a crude extract extracted by using any one selected from the group consisting of a nonpolar solvent of ether, chloroform, benzene, hexane or dichloromethane, and a mixed solvent thereof. According to claim 4, The genus extract is a fraction fractionated by using any one selected from the group consisting of a non-polar solvent of ether, chloroform, benzene, hexane or dichloromethane and a mixed solvent thereof as the crude extract, Antimicrobial composition. At least one preventive or therapeutic composition selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition of any one of claims 1 to 5 as an active ingredient. At least one preventive or improved cleaning composition selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition of any one of claims 1 to 5 as an active ingredient. At least one preventive or improved cosmetic composition selected from the group consisting of dandruff and seborrheic dermatitis comprising the antimicrobial composition of any one of claims 1 to 5 as an active ingredient.

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