KR100786564B1 - Preparing method of composition of natural preservatives - Google Patents

Abstract

A method for preparing a natural preservative is provided to obtain the preservative which is very adequate for preserving cosmetics directly contacted with human body due to no side effects and shows excellent preservative effect on the cosmetics easy to be deteriorated. A method for preparing a natural preservative comprises the steps of: (a) preparing an extract of Scutellaria baicalensis, an extract of Paeonia Suffruticosa Andrews, and an extract of grapefruit seed, respectively; (b) mixing 10-40 parts by weight of the extract of Scutellaria baicalensis, 10-30 parts by weight of the extract of Paeonia Suffruticosa Andrews and 15-40 parts by weight of the extract of grapefruit seed; (c) fermenting the mixture for 5-7 days at room temperature to prepare a fermented composition including liquid phase and solid matter; (d) solid/liquid-separating the fermented composition to recover a vegetable fermented solution; and (e) ripening the vegetable fermented solution for 5-7 days at room temperature.

Description

Production method of natural preservative composition {PREPARING METHOD OF COMPOSITION OF NATURAL PRESERVATIVES}

1 is a flow chart of a method for producing a natural preservative composition according to an embodiment of the present invention

The present invention relates to a method for preparing a natural preservative composition that provides an antiseptic effect to cosmetics, pharmaceuticals, detergents, etc. More specifically, since it is made of only natural materials and has no side effects on the human body, it is very effective for antiseptic treatment of cosmetics directly in contact with the human body. It relates to a method for producing a natural preservative composition that is suitable and excellent in antiseptic effect on cosmetics.

As income increases, cleanliness is emphasized, and interest in appearance increases, the amount of various cosmetics such as skins, lotions, creams, essences, shampoos, soaps, toothpastes, and cleaners is increasing.

Such cosmetics are products that are in direct contact with the human body, and after opening the package, it is used for a relatively long time, and since it is easy to be deteriorated due to the characteristics of the product, it is essential to preservative treatment using a preservative. In particular, in recent years, the tendency to focus on health and well-being is increased, and since various cosmetics are mainly manufactured using natural materials to meet these demands, the possibility of cosmetic deterioration and corruption is increasing.

Therefore, conventionally, by adding a synthetic preservative to a certain amount of cosmetics by synthesizing chemicals, it is possible to prevent alteration and decay of the cosmetics. Although these synthetic preservatives have excellent antiseptic effect on cosmetics, they are chemical products made by synthesizing chemicals and thus affect the health of cosmetic users. There is a problem.

Accordingly, an object of the present invention is to provide a method for producing a natural preservative composition excellent in the preservative effect on cosmetics, because it is made of a natural material has no side effects on the human body is suitable for direct contact with the human body.

In order to achieve the above object of the present invention, an embodiment of the present invention comprises the steps of forming a golden extract, bark extract, grapefruit seed extract; Forming a mixture by mixing 10 to 40 parts by weight of the golden extract, 10 to 30 parts by weight of bark skin extract, 15 to 40 parts by weight of grapefruit seed extract; Fermenting the mixture at room temperature for 5 to 7 days to form a fermentation composition; Recovering the vegetable fermentation broth by solid / liquid separation of the fermentation composition; And it provides a method for producing a natural preservative composition comprising the step of aging the vegetable fermentation broth at room temperature for 5 to 7 days.

The natural preservative composition prepared according to the present invention is made of only natural materials, so there is no side effect on the human body, so it is very suitable for antiseptic treatment of cosmetics in direct contact with the human body, and has an effect of excellent antiseptic effect on cosmetics that are easily deteriorated. .

An embodiment of a method for producing a natural preservative composition of the present invention will be described in detail with reference to the drawings.

1 illustrates a procedure of a method of preparing a natural preservative composition according to an embodiment of the present invention.

Referring to Figure 1, first an embodiment of the method for producing a natural preservative composition of the present invention includes the step of forming a golden extract, bark skin extract, grapefruit seed extract (step S10).

Gold, which is a raw material of the natural preservative composition of the present invention, grows wild or grows as a medicinal plant in mountain meadows of Korea, China, Mongolia and eastern Siberia, and has a height of 20 to 60 cm, and purple flowers bloom in July and August. The fruit is a form of abandonment that fruit in September. In oriental medicine, roots are used as antipyretic, diuretic, branch, edema and anti-inflammatory drugs.

In the present invention, in the present invention, which is such a herbal medicine, the roots of the gold, which is the herbal medicine, are pulverized to form a golden pulverized product, and the mixture is formed by using a liquid golden extract obtained by pressing the golden pulverized product.

In addition, the bark of the natural preservative composition is a medicinal herb made from the peony root of peony peony, has a peculiar smell, taste a bit bitter and very cold. Bark skin is used for menstrual disorders due to blood fever, menstrual pain, bruising, bleeding, nosebleeds, spots, swelling of bones due to fever, increased blood pressure, blood loss, bruises, anti-inflammatory pain, boil treatment, early appendicitis And get rid of heartburn. As pharmacological action, analgesic, sedation, antipyretic, anticonvulsion, anti-inflammatory, anti-thrombotic, anti-allergic, gastric secretion suppression, uterine mucosal congestion, antibacterial action has been reported.

In the present invention, the bark of the root of the peony is pulverized to form a shredded bark, and the mixture of the bark dermis obtained from the liquid obtained by pressing the shredded bark.

In addition, grapefruit seed, which is a raw material of the natural preservative composition, is a seed of grapefruit which is a fruit, and is useful as a fungicide and a natural food preservative because it has a growth inhibitory and bactericidal action against various bacteria.

In the present invention, the grapefruit seed is pulverized to form a grapefruit seed pulverized, and the mixture is constituted using a liquid grapefruit seed extract obtained by pressing the grapefruit seed crushed.

In addition, one embodiment of the method for producing a natural preservative composition of the present invention comprises the steps of mixing the golden extract 10 to 40 parts by weight, 10 to 30 parts by weight of bark extract, grapefruit seed extract 15 to 40 parts by weight to form a mixture (Step S20).

When the golden extract, the bark skin extract, and the grapefruit seed extract, which are formed separately in step S10, are mixed in a predetermined amount, each of the extracts synergizes with each other to form a mixture which is an intermediate having excellent antiseptic function, fermenting the mixture, Solid / liquid separation, and maturation to form the natural preservative composition. That is, the natural preservative composition of the present invention is to provide an excellent antiseptic function by synergistic interaction with each of the extracts only when using all of the golden extract, bark skin extract, and grapefruit seed extract.

In the present invention, the golden roots are pulverized to form a golden pulverized product, and the mixture contains 10 to 40 parts by weight of the liquid golden extract obtained by pressing the golden pulverized product.

If the content of the golden extract contained in the mixture is less than 10 parts by weight of gold, the inherent pharmacological action of the gold is lowered. If the content of the golden extract exceeds 40 parts by weight, the content of the golden extract is relatively excessive, so the extract of grapefruit and grapefruit seeds Synergy with is inhibited.

In addition, the bark of the root of the peony is pulverized to form a pulverulent pulverulum, and containing 10 to 30 parts by weight of the liquid pulverulent extract obtained by pressing the pulverulent pulverulent to form a mixture.

If the content of the bark extract contained in the mixture is less than 10 parts by weight, the inherent pharmacological action of the bark skin is lowered. If the content of the bark extract exceeds 30 parts by weight, the content of the bark extract is relatively excessive. Synergy with is inhibited.

In addition, the grapefruit seed is pulverized to form a grapefruit seed pulverized, and containing 15 to 40 parts by weight of the liquid grapefruit seed extract obtained by pressing the grapefruit seed pulverized to form a mixture.

When the content of the grapefruit seed extract contained in the mixture is less than 15 parts by weight, the growth inhibition and bactericidal activity of various natural bacteria of the natural preservative composition comprising the mixture is lowered, and when the content of the grapefruit seed extract exceeds 40 parts by weight, the grapefruit seed extract Relatively high content of is inhibited synergy with golden extract, extract of the bark.

In addition, one embodiment of the method for producing a natural preservative composition of the present invention includes the step of fermenting the mixture at room temperature for 5 to 7 days to form a fermentation composition (step S30).

That is, in the step of forming the mixture, the mixture of 10 to 40 parts by weight of golden extract, 10 to 30 parts by weight of bark extract, and 15 to 40 parts by weight of grapefruit seed extract is left to ferment for 5 to 7 days at room temperature.

In the step of fermenting the intermediate mixture, the components of the golden extract, bark skin extract, and grapefruit seed extract synergize with each other to maximize the antibacterial function and various pharmacological action of the fermentation composition, and at the same time the solid content of the fermentation composition It will settle.

If the period of fermentation of the mixture is less than 5 days, the mixture does not ferment properly, and if the period of fermentation of the mixture exceeds 7 days, the mixture may be over fermented.

In addition, one embodiment of the method for producing a natural preservative composition of the present invention includes the step of recovering the vegetable fermentation broth by solid / liquid separation of the fermentation composition (step S40).

Specifically, in the present invention, the golden, bark bark, grapefruit seeds with low moisture content are crushed and compressed, respectively, to obtain a golden extract, bark bark extract, and grapefruit seed extract. Solids are generated in the fermentation composition. If solid content remains in the fermentation composition, it may affect the quality or the commerciality of the cosmetic containing the natural preservative composition formed from the fermentation composition. In order to prevent this, by solid / liquid separation of the fermentation composition precipitated solid in the previous step S30 by a method such as filtration, to remove the solids from the fermentation composition and to recover the vegetable fermentation broth as a liquid component in the production of a natural preservative composition use.

In addition, one embodiment of the method for producing a natural preservative composition of the present invention includes the step of aging the vegetable fermentation broth at room temperature for 5 to 7 days (step S50).

That is, the natural preservative composition of the present invention is prepared by aging the recovered vegetable fermentation broth at room temperature for 5 to 7 days.

In the step of aging the vegetable fermentation broth as described above, an additional synergy occurs between the golden component, bark skin component, and grapefruit seed components contained in the vegetable fermentation broth to maximize the antiseptic effect of the vegetable fermentation broth.

If the period of ripening the vegetable fermentation broth is less than 5 days, the vegetable fermentation broth is not properly aged, and if the period of fermentation of the vegetable fermentation broth exceeds 7 days, the vegetable fermentation broth is no longer aged.

Natural preservative composition is configured as described above is made of only natural ingredients of golden, bark skin, grapefruit seeds, so there is no side effect on the human body is very suitable for antiseptic treatment of cosmetics in direct contact with the human body.

In addition, by mixing a predetermined amount of a pharmaceutically acceptable carrier such as purified water, stearic acid, stearyl alcohol, butylene glycol, monostearic acid glycol, potassium hydroxide, squalane, glycerin, cholesterol, and carboxyvinyl polymer in the natural preservative composition It is possible to form pharmaceutical compositions of natural preservative compositions.

In the manufacturing process of various cosmetics such as skins, lotions, creams, essences, shampoos, soaps, toothpastes, and detergents, a certain amount of a natural preservative composition is added to the cosmetics as it is, or a pharmaceutical composition of the natural preservative composition is added to the cosmetics. By adding a certain amount, the cosmetic treatment is antiseptic.

Hereinafter, the present invention will be described in detail with reference to a preferred embodiment. However, the following Example is for illustrating this invention concretely, It does not limit only to this.

<Example>

1. The root of the gold, which is a herbal medicine, was pulverized to form a golden powder, and the golden powder was compressed to obtain a liquid golden extract.

The bark of the root of the peony was pulverized to form a shredded bark, and the shredded bark was compressed to obtain a liquid bark extract.

Grapefruit seeds were ground to form a grapefruit seed mill, and the grapefruit seed mill was pressed to obtain a liquid grapefruit seed extract.

2. 250 g of the golden extract, 200 g of bark extract, and 300 g of grapefruit seed extract were mixed in a reactor and stirred to form a mixture.

3. The mixture was left at room temperature for 6 days to ferment to form a fermentation composition.

4. The fermentation composition was filtered to remove solids and the vegetable fermentation broth as a liquid component was recovered.

5. The natural preservative composition was prepared by aging the vegetable fermentation broth at room temperature for 6 days.

<Comparative Example 1>

1. The root of the gold, which is a herbal medicine, was pulverized to form a golden powder, and the golden powder was compressed to obtain a liquid golden extract.

2. The golden extract was left to stand at room temperature for 6 days to ferment.

3. The fermented golden extract was filtered to remove solids, liquid components were recovered, and the liquid components were aged at room temperature for 6 days to form a golden fermentation broth.

<Comparative Example 2>

1. The pulverized bark skin was formed to form a pulverized bark, and the pulverized bark was crushed to obtain a liquid bark extract.

2. Fermented bark extract was left at room temperature for 6 days.

3. The fermented bark extract was filtered to remove solids, liquid components were recovered, and the liquid components were aged at room temperature for 6 days to form a bark fermentation broth.

<Comparative Example 3>

1. Grapefruit seeds were ground to form a grapefruit seed mill, and the grapefruit seed mill was pressed to obtain a liquid grapefruit seed extract.

2. The grapefruit seed extract was left to ferment for 6 days at room temperature.

3. The fermented grapefruit seed extract was filtered to remove solids and recovered liquid components, and the liquid components were aged at room temperature for 6 days to form a grapefruit seed fermentation broth.

<Comparative Example 4>

1. 250 g of the golden extract of Comparative Example 1 and 200 g of the bark extract of Comparative Example 2 were mixed and stirred in a reactor to form a first mixed extract.

2. The first mixed extract was left to ferment for 6 days.

3. The fermented first mixed extract was filtered to remove solids, liquid components were recovered, and the liquid components were aged at room temperature for 6 days to form a first mixed fermentation broth.

<Comparative Example 5>

1. 250 g of golden extract of Comparative Example 1 and 300 g of grapefruit seed extract of Comparative Example 3 were mixed and stirred in a reactor to form a second mixed extract.

2. The second mixed extract was left to ferment for 6 days.

3. The fermented second mixed extract was filtered to remove solids, recover liquid components, and the liquid components were aged at room temperature for 6 days to form a second mixed fermentation broth.

<Comparative Example 6>

1. 200 g of bark extract of Comparative Example 2 and 300 g of grapefruit seed extract of Comparative Example 3 were mixed and stirred in a reactor to form a third mixed extract.

2. The third mixed extract was left to ferment for 6 days.

3. The fermented third mixed extract was filtered to remove solids, and the liquid component was recovered, and the liquid component was aged at room temperature for 6 days to form a third mixed fermentation solution.

The natural preservative composition of the above embodiment, the fermentation broths of Comparative Examples 1 to 6, conventional synthetic preservatives (methyl para benzene (10% by weight, propyl para-benzene) 4% by weight, phenoxy The antiseptic effect measurement experiment was carried out to the cosmetics added with ethanol (containing 10% by weight of phenoxy ethanol) and the unpreserved cosmetics as follows.

Experimental Example 1

The preservative effect measurement experiments were performed on the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the skin cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to skin cosmetics to form samples, and each of the first strains, Staphylococcus aureus ATCC6538 7.0 x 10 ^The inoculation effect was compared by inoculating ⁷ / g and measuring the number of viable cells at a certain time point for 4 weeks.

Table 1 shows the results of the antiseptic effect measurement experiment.

Table 1 shows the simultaneous inoculation of the first strain into the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the samples to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 1> Comparison result of preservative effect against the first strain of skin cosmetic

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 7.0 x 10 ⁷ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 7.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 7.0 x 10 ⁷ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 7.0 x 10 ⁷ 5.0 x 10 ⁶ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 7.0 x 10 ⁷ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ Comparative Example 4 7.0 x 10 ⁷ 6.0 x 10 ⁶ 1.0 x 10 ⁴ 7.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 7.0 x 10 ⁷ 3.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 7.0 x 10 ⁷ 4.0 x 10 ⁴ 2.0 x 10 ² 97 57 70 Preservative Free 7.0 x 10 ⁷ 9.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x10 ⁷

In Table 1, the antiseptic function of the natural preservative composition of the present invention on the skin cosmetic was found to be much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 2

Preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, the lotion cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the lotion cosmetics to form samples, and the first strain Staphylococcus aureus ATCC6538 7.0 x 10 for each sample. ^The inoculation effect was compared by inoculating ⁷ / g and measuring the number of viable cells at a certain time point for 4 weeks.

Table 2 shows the results of the antiseptic effect measurement experiment.

Table 2 shows the simultaneous inoculation of the first strain into the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added, respectively. At the same time, the live viable cell count was simultaneously measured.

<Table 2> Comparison results of antiseptic effect on the first strain of lotion cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 7.0 x 10 ⁷ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 7.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 7.0 x 10 ⁷ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 7.0 x 10 ⁷ 5.0 x 10 ⁶ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 7.0 x 10 ⁷ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ Comparative Example 4 7.0 x 10 ⁷ 6.0 x 10 ⁶ 1.0 x 10 ⁴ 7.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 7.0 x 10 ⁷ 3.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 7.0 x 10 ⁷ 4.0 x 10 ⁴ 2.0 x 10 ² 97 57 70 Preservative Free 7.0 x 10 ⁷ 9.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 2, the antiseptic function of the natural preservative composition of the present invention for the lotion cosmetics was much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 3

The preservative effect measurement experiments were performed for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the essence cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the essence cosmetics to form samples, and the first strain Staphylococcus aureus ATCC6538 7.0 x 10 for each sample. ^The inoculation effect was compared by inoculating ⁷ / g and measuring the number of viable cells at a certain time point for 4 weeks.

Table 3 shows the results of the antiseptic effect measurement experiment.

Table 3 shows the simultaneous inoculation of the first strain into the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added, respectively. At the same time, the live viable cell count was simultaneously measured.

<Table 3> Comparison result of preservative effect on the first strain of essence cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 7.0 x 10 ⁷ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 7.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 7.0 x 10 ⁷ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 7.0 x 10 ⁷ 5.0 x 10 ⁶ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 7.0 x 10 ⁷ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ Comparative Example 4 7.0 x 10 ⁷ 6.0 x 10 ⁶ 1.0 x 10 ⁴ 7.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 7.0 x 10 ⁷ 3.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 7.0 x 10 ⁷ 4.0 x 10 ⁴ 2.0 x 10 ² 97 57 70 Preservative Free 7.0 x 10 ⁷ 9.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 3, the antiseptic function of the natural preservative composition of the present invention on the essence cosmetics was much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 4

The preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the cream cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to cream cosmetics to form samples, and the first strain Staphylococcus aureus ATCC6538 7.0 x 10 for each sample. ⁷ / g was inoculated and the number of viable cells was measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 4 shows the results of the antiseptic effect measurement experiment.

Table 4 shows the simultaneous inoculation of the first strain into the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added, respectively. At the same time, the live viable cell count was simultaneously measured.

<Table 4> Comparison result of preservative effect against the first strain of cream cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 7.0 x 10 ⁷ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 7.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 7.0 x 10 ⁷ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 7.0 x 10 ⁷ 5.0 x 10 ⁶ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 7.0 x 10 ⁷ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ Comparative Example 4 7.0 x 10 ⁷ 6.0 x 10 ⁶ 1.0 x 10 ⁴ 7.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 7.0 x 10 ⁷ 3.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 7.0 x 10 ⁷ 4.0 x 10 ⁴ 2.0 x 10 ² 97 57 70 Preservative Free 7.0 x 10 ⁷ 9.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 4, the antiseptic function of the natural preservative composition of the present invention on the cream cosmetics was much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 5

Preservative effect measurement experiments were performed on the natural preservative composition prepared according to the above embodiment, the fermentation broth prepared according to Comparative Examples 1 to 6, and the skin cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to skin cosmetics, respectively, to form samples, and each sample was Escherichia coli ATCC8739 5.0 x. 10 ⁷ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 5 shows the results of the antiseptic effect measurement experiment.

Table 5 simultaneously inoculates the second strain on the sample to which the natural antiseptic composition is added, the sample to which the conventional synthetic preservative is added, the samples to which the fermentation broths of Comparative Examples 1 to 6 are added, and the sample to which the preservative is not added. At the same time, the live viable cell count was simultaneously measured.

<Table 5> Comparison results of preservative effect against the second strain of skin cosmetic

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁷ 3.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁷ 3.0 x 10 ⁴ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁷ 4.0 x 10 ⁶ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 6.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 2 5.0 x 10 ⁷ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 6.0 x 10 ⁴ 2.0 x 10 ⁴ 1.0 x 10 ⁴ Comparative Example 3 5.0 x 10 ⁷ 6.0 x 10 ⁶ 5.0 x 10 ⁴ 1.0 x 10 ⁴ 4.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 4 5.0 x 10 ⁷ 2.0 x 10 ⁶ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 1.0 x 10 ⁴ 6.0 x 10 ⁴ Comparative Example 5 5.0 x 10 ⁷ 6.0 x 10 ⁶ 6.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ 9.0 x 10 ⁴ Comparative Example 6 5.0 x 10 ⁷ 7.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 5.0 x 10 ⁷ 3.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 5, the antiseptic function of the natural preservative composition of the present invention on the skin cosmetic was found to be much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 6

Preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, the lotion cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the lotion cosmetics to form samples, and each sample was E. coli Escherichia coli ATCC8739 5.0 x 10 ^The inoculation effect was compared by inoculating ⁷ / g and measuring the number of viable cells at a certain time point for 4 weeks.

Table 6 shows the results of the antiseptic effect measurement experiment.

Table 6 shows the simultaneous inoculation of the second strain into the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 6> Comparison results of preservative effect on the second strain of lotion cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁷ 3.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁷ 3.0 x 10 ⁴ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁷ 4.0 x 10 ⁶ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 6.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 2 5.0 x 10 ⁷ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 6.0 x 10 ⁴ 2.0 x 10 ⁴ 1.0 x 10 ⁴ Comparative Example 3 5.0 x 10 ⁷ 6.0 x 10 ⁶ 5.0 x 10 ⁴ 1.0 x 10 ⁴ 4.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 4 5.0 x 10 ⁷ 2.0 x 10 ⁶ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 1.0 x 10 ⁴ 6.0 x 10 ⁴ Comparative Example 5 5.0 x 10 ⁷ 6.0 x 10 ⁶ 6.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ 9.0 x 10 ⁴ Comparative Example 6 5.0 x 10 ⁷ 7.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 5.0 x 10 ⁷ 3.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 6, the antiseptic function of the natural preservative composition of the present invention for the lotion cosmetics was much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 7

The preservative effect measurement experiments were performed for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the essence cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the essence cosmetics to form samples, and the second strain E. coli Escherichia coli ATCC8739 5.0 x for each sample. 10 ⁷ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 7 shows the results of the antiseptic effect measurement experiment.

Table 7 shows the simultaneous inoculation of the second strain on the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the samples to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 7> Comparison result of preservative effect for the second strain of essence cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁷ 3.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁷ 3.0 x 10 ⁴ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁷ 4.0 x 10 ⁶ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 6.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 2 5.0 x 10 ⁷ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 6.0 x 10 ⁴ 2.0 x 10 ⁴ 1.0 x 10 ⁴ Comparative Example 3 5.0 x 10 ⁷ 6.0 x 10 ⁶ 5.0 x 10 ⁴ 1.0 x 10 ⁴ 4.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 4 5.0 x 10 ⁷ 2.0 x 10 ⁶ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 1.0 x 10 ⁴ 6.0 x 10 ⁴ Comparative Example 5 5.0 x 10 ⁷ 6.0 x 10 ⁶ 6.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ 9.0 x 10 ⁴ Comparative Example 6 5.0 x 10 ⁷ 7.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 5.0 x 10 ⁷ 3.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 7, the preservative function of the natural preservative composition of the present invention for the essence cosmetics was much superior to the conventional synthetic preservatives, comparative examples, no preservatives.

Experimental Example 8

The preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the cream cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to cream cosmetics to form samples, and each sample was E. coli Escherichia coli ATCC8739 5.0 x 10 ⁷ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 8 shows the results of the antiseptic effect measurement experiment.

Table 8 shows the simultaneous inoculation of the second strain on the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the samples to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 8> Comparison results of antiseptic effect on the second strain of cream cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁷ 3.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁷ 3.0 x 10 ⁴ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁷ 4.0 x 10 ⁶ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 6.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 2 5.0 x 10 ⁷ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 6.0 x 10 ⁴ 2.0 x 10 ⁴ 1.0 x 10 ⁴ Comparative Example 3 5.0 x 10 ⁷ 6.0 x 10 ⁶ 5.0 x 10 ⁴ 1.0 x 10 ⁴ 4.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 4 5.0 x 10 ⁷ 2.0 x 10 ⁶ 7.0 x 10 ⁴ 3.0 x 10 ⁴ 1.0 x 10 ⁴ 6.0 x 10 ⁴ Comparative Example 5 5.0 x 10 ⁷ 6.0 x 10 ⁶ 6.0 x 10 ⁴ 7.0 x 10 ⁴ 8.0 x 10 ⁴ 9.0 x 10 ⁴ Comparative Example 6 5.0 x 10 ⁷ 7.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 5.0 x 10 ⁷ 3.0 x 10 ⁸ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 8, the antiseptic function of the natural preservative composition of the present invention on the cream cosmetics was much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 9

Preservative effect measurement experiments were performed on the natural preservative composition prepared according to the above embodiment, the fermentation broth prepared according to Comparative Examples 1 to 6, and the skin cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to skin cosmetics to form samples, and the third strain Pseudomonas aeruginosa ATCC9027 4.0 x for each sample. 10 ⁷ / g was inoculated and the number of viable cells at a certain time point for 4 weeks to measure the antiseptic effect.

Table 9 shows the results of the antiseptic effect measurement experiment.

Table 9 shows simultaneous inoculation of the third strain into a sample to which the natural antiseptic composition was added, a sample to which a conventional synthetic preservative was added, a sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and a sample to which no preservative was added. At the same time, the live viable cell count was simultaneously measured.

<Table 9> Comparison results of preservative effect against the third strain of skin cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 4.0 x 10 ⁷ 2.0 x 10 ³ 10 0 0 0 Synthetic preservative 4.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 4.0 x 10 ⁷ 2.0 x 10 ⁶ 3.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 2 4.0 x 10 ⁷ 3.0 x 10 ⁶ 1.0 x 10 ⁵ 5.0 x 10 ⁵ 3.0 x 10 ⁵ 1.0 x 10 ⁵ Comparative Example 3 4.0 x 10 ⁷ 5.0 x 10 ⁶ 2.0 x 10 ⁵ 4.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ Comparative Example 4 4.0 x 10 ⁷ 3.0 x 10 ⁶ 7.0 x 10 ⁵ 3.0 x 10 ⁵ 5.0 x 10 ⁵ 7.0 x 10 ⁵ Comparative Example 5 4.0 x 10 ⁷ 7.0 x 10 ⁶ 5.0 x 10 ⁴ 7.0 x 10 ⁵ 8.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 6 4.0 x 10 ⁷ 4.0 x 10 ⁶ 2.0 x 10 ² 80 60 70 Preservative Free 4.0 x 10 ⁷ 6.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 9, the antiseptic function of the natural preservative composition of the present invention on the skin cosmetic was found to be much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 10

Preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, the lotion cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the lotion cosmetics, respectively, to form samples, and each sample was Pseudomonas aeruginosa ATCC9027 4.0 x 10 ⁷ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 10 shows the results of the antiseptic effect measurement experiment.

Table 10 shows simultaneous inoculation of the third strain into a sample to which the natural antiseptic composition was added, a sample to which a conventional synthetic preservative was added, a sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and a sample to which no preservative was added. At the same time, the live viable cell count was simultaneously measured.

<Table 10> Comparison results of preservative effect on the third strain of lotion cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 4.0 x 10 ⁷ 2.0 x 10 ³ 10 0 0 0 Synthetic preservative 4.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 4.0 x 10 ⁷ 2.0 x 10 ⁶ 3.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 2 4.0 x 10 ⁷ 3.0 x 10 ⁶ 1.0 x 10 ⁵ 5.0 x 10 ⁵ 3.0 x 10 ⁵ 1.0 x 10 ⁵ Comparative Example 3 4.0 x 10 ⁷ 5.0 x 10 ⁶ 2.0 x 10 ⁵ 4.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ Comparative Example 4 4.0 x 10 ⁷ 3.0 x 10 ⁶ 7.0 x 10 ⁵ 3.0 x 10 ⁵ 5.0 x 10 ⁵ 7.0 x 10 ⁵ Comparative Example 5 4.0 x 10 ⁷ 7.0 x 10 ⁶ 5.0 x 10 ⁴ 7.0 x 10 ⁵ 8.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 6 4.0 x 10 ⁷ 4.0 x 10 ⁶ 2.0 x 10 ² 80 60 70 Preservative Free 4.0 x 10 ⁷ 6.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 10, the antiseptic function of the natural preservative composition of the present invention for the lotion cosmetics was much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 11

The preservative effect measurement experiments were performed for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the essence cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the essence cosmetics to form samples, and the third strain Pseudomonas aeruginosa ATCC9027 4.0 x for each sample. 10 ⁷ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 11 shows the results of the antiseptic effect measurement experiment.

Table 11 shows simultaneous inoculation of the third strain into a sample to which the natural antiseptic composition was added, a sample to which a conventional synthetic preservative was added, a sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and a sample to which a preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 11> Comparison results of preservative effect on the third strain of essence cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 4.0 x 10 ⁷ 2.0 x 10 ³ 10 0 0 0 Synthetic preservative 4.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 4.0 x 10 ⁷ 2.0 x 10 ⁶ 3.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 2 4.0 x 10 ⁷ 3.0 x 10 ⁶ 1.0 x 10 ⁵ 5.0 x 10 ⁵ 3.0 x 10 ⁵ 1.0 x 10 ⁵ Comparative Example 3 4.0 x 10 ⁷ 5.0 x 10 ⁶ 2.0 x 10 ⁵ 4.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ Comparative Example 4 4.0 x 10 ⁷ 3.0 x 10 ⁶ 7.0 x 10 ⁵ 3.0 x 10 ⁵ 5.0 x 10 ⁵ 7.0 x 10 ⁵ Comparative Example 5 4.0 x 10 ⁷ 7.0 x 10 ⁶ 5.0 x 10 ⁴ 7.0 x 10 ⁵ 8.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 6 4.0 x 10 ⁷ 4.0 x 10 ⁶ 2.0 x 10 ² 80 60 70 Preservative Free 4.0 x 10 ⁷ 6.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 11, the antiseptic function of the natural preservative composition of the present invention on the essence cosmetics was much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 12

The preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the cream cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to cream cosmetics to form samples, and the third strain Pseudomonas aeruginosa ATCC9027 4.0 x for each sample. 10 ⁷ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 12 shows the results of the antiseptic effect measurement experiment.

Table 12 shows the simultaneous inoculation of the third strain into the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the samples to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 12> Comparison results of antiseptic effect on the third strain of cream cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 4.0 x 10 ⁷ 2.0 x 10 ³ 10 0 0 0 Synthetic preservative 4.0 x 10 ⁷ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 4.0 x 10 ⁷ 2.0 x 10 ⁶ 3.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 2 4.0 x 10 ⁷ 3.0 x 10 ⁶ 1.0 x 10 ⁵ 5.0 x 10 ⁵ 3.0 x 10 ⁵ 1.0 x 10 ⁵ Comparative Example 3 4.0 x 10 ⁷ 5.0 x 10 ⁶ 2.0 x 10 ⁵ 4.0 x 10 ⁵ 2.0 x 10 ⁵ 3.0 x 10 ⁵ Comparative Example 4 4.0 x 10 ⁷ 3.0 x 10 ⁶ 7.0 x 10 ⁵ 3.0 x 10 ⁵ 5.0 x 10 ⁵ 7.0 x 10 ⁵ Comparative Example 5 4.0 x 10 ⁷ 7.0 x 10 ⁶ 5.0 x 10 ⁴ 7.0 x 10 ⁵ 8.0 x 10 ⁵ 4.0 x 10 ⁵ Comparative Example 6 4.0 x 10 ⁷ 4.0 x 10 ⁶ 2.0 x 10 ² 80 60 70 Preservative Free 4.0 x 10 ⁷ 6.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 12, the antiseptic function of the natural preservative composition of the present invention on the cream cosmetics was much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 13

Preservative effect measurement experiments were performed on the natural preservative composition prepared according to the above embodiment, the fermentation broth prepared according to Comparative Examples 1 to 6, and the skin cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to skin cosmetics to form samples, and the fourth strain Candida albicans ATCC10231 8.0 x 10 for each sample. ⁶ / g was inoculated and the number of viable cells was measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 13 shows the results of the antiseptic effect measurement experiment.

Table 13 shows the simultaneous inoculation of the fourth strain in the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 13> Experimental results of antiseptic effect on the fourth strain of skin cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 8.0 x 10 ⁶ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 8.0 x 10 ⁶ 4.0 x 10 ³ 30 0 0 0 Comparative Example 1 8.0 x 10 ⁶ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 8.0 x 10 ⁶ 5.0 x 10 ⁵ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 7.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 8.0 x 10 ⁶ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 4 8.0 x 10 ⁶ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 3.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 8.0 x 10 ⁶ 2.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 8.0 x 10 ⁶ 2.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 8.0 x 10 ⁶ 8.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 13, the antiseptic function of the natural preservative composition of the present invention on the skin cosmetic was found to be much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 14

Preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, the lotion cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the lotion cosmetics to form samples, and the fourth strain Candida albicans ATCC10231 8.0 x 10 for each sample. ⁶ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 14 shows the results of the antiseptic effect measurement experiment.

Table 14 shows the simultaneous inoculation of the fourth strain on the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the samples to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 14> Comparison results of antiseptic effect on the fourth strain of lotion cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 8.0 x 10 ⁶ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 8.0 x 10 ⁶ 4.0 x 10 ³ 30 0 0 0 Comparative Example 1 8.0 x 10 ⁶ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 8.0 x 10 ⁶ 5.0 x 10 ⁵ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 7.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 8.0 x 10 ⁶ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 4 8.0 x 10 ⁶ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 3.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 8.0 x 10 ⁶ 2.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 8.0 x 10 ⁶ 2.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 8.0 x 10 ⁶ 8.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 14, the antiseptic function of the natural preservative composition of the present invention on the lotion cosmetics was much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 15

The preservative effect measurement experiments were performed for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the essence cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the essence cosmetics to form samples, and the fourth strain Candida albicans ATCC10231 8.0 x 10 for each sample. ⁶ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 15 shows the results of the antiseptic effect measurement experiment.

Table 15 shows the simultaneous inoculation of the fourth strain on the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 15> Comparison results of antiseptic effect on the fourth strain of essence cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 8.0 x 10 ⁶ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 8.0 x 10 ⁶ 4.0 x 10 ³ 30 0 0 0 Comparative Example 1 8.0 x 10 ⁶ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 8.0 x 10 ⁶ 5.0 x 10 ⁵ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 7.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 8.0 x 10 ⁶ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 4 8.0 x 10 ⁶ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 3.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 8.0 x 10 ⁶ 2.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 8.0 x 10 ⁶ 2.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 8.0 x 10 ⁶ 8.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 15, the antiseptic function of the natural preservative composition of the present invention on the essence cosmetics was much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 16

The preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the cream cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the cream cosmetics to form samples, and the fourth strain Candida albicans ATCC10231 8.0 x 10 for each sample. ⁶ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 16 shows the results of the antiseptic effect measurement experiment.

Table 16 shows the simultaneous inoculation of the fourth strain on the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the samples to which the fermentation solutions of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 16> Comparative Experiment Results of Preservative Effect against Cream 4 Strains

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 8.0 x 10 ⁶ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 8.0 x 10 ⁶ 4.0 x 10 ³ 30 0 0 0 Comparative Example 1 8.0 x 10 ⁶ 4.0 x 10 ⁵ 3.0 x 10 ⁴ 4.0 x 10 ⁴ 5.0 x 10 ⁴ 7.0 x 10 ⁴ Comparative Example 2 8.0 x 10 ⁶ 5.0 x 10 ⁵ 4.0 x 10 ⁴ 7.0 x 10 ⁴ 7.0 x 10 ⁴ 5.0 x 10 ⁴ Comparative Example 3 8.0 x 10 ⁶ 5.0 x 10 ⁵ 3.0 x 10 ⁴ 2.0 x 10 ⁴ 7.0 x 10 ⁴ 3.0 x 10 ⁴ Comparative Example 4 8.0 x 10 ⁶ 7.0 x 10 ⁶ 1.0 x 10 ⁴ 3.0 x 10 ³ 3.0 x 10 ³ 9.0 x 10 ³ Comparative Example 5 8.0 x 10 ⁶ 2.0 x 10 ⁴ 6.0 x 10 ³ 2.0 x 10 ³ 7.0 x 10 ³ 4.0 x 10 ³ Comparative Example 6 8.0 x 10 ⁶ 2.0 x 10 ⁴ 2.0 x 10 ² 90 50 70 Preservative Free 8.0 x 10 ⁶ 8.0 x 10 ⁷ 5.0 x 10 ⁷ 6.0 x 10 ⁷ 8.0 x 10 ⁷ 7.0 x 10 ⁷

In Table 16, the antiseptic function of the natural preservative composition of the present invention on the cream cosmetics was much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 17

Preservative effect measurement experiments were performed on the natural preservative composition prepared according to the above embodiment, the fermentation broth prepared according to Comparative Examples 1 to 6, and the skin cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to skin cosmetics, respectively, to form samples, and asperfillus niger ATCC90906 5.0 x 10 for each sample. ⁵ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 17 shows the results of the antiseptic effect measurement experiment.

Table 17 shows the fifth strain simultaneously with the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. Inoculation is performed, and the number of live bacteria is measured at the same time.

<Table 17> Comparison results of preservative effect on the fifth strain of skin cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁵ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁶ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁵ 4.0 x 10 ⁵ 3.0 x 10 ³ 4.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Comparative Example 2 5.0 x 10 ⁵ 2.0 x 10 ⁴ 6.0 x 10 ³ 3.0 x 10 ³ 2.0 x 10 ³ 3.0 x 10 ³ Comparative Example 3 5.0 x 10 ⁵ 6.0 x 10 ⁴ 4.0 x 10 ³ 4.0 x 10 ³ 7.0 x 10 ³ 6.0 x 10 ³ Comparative Example 4 5.0 x 10 ⁵ 8.0 x 10 ⁴ 8.0 x 10 ³ 7.0 x 10 ³ 3.0 x 10 ³ 7.0 x 10 ³ Comparative Example 5 5.0 x 10 ⁵ 2.0 x 10 ⁴ 5.0 x 10 ³ 5.0 x 10 ³ 8.0 x 10 ³ 1.0 x 10 ³ Comparative Example 6 5.0 x 10 ⁵ 6.0 x 10 ⁴ 3.0 x 10 ³ 6.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Preservative Free 5.0 x 10 ⁵ 6.0 x 10 ⁶ 5.0 x 10 ⁶ 6.0 x 10 ⁵ 8.0 x 10 ⁵ 7.0 x 10 ⁵

In Table 17, the antiseptic function of the natural preservative composition of the present invention on the skin cosmetic was found to be much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 18

Preservative effect measurement experiments were carried out for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, the lotion cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the lotion cosmetics, respectively, to form samples, and asperfillus niger ATCC90906 5.0 x 10 for each sample. ⁵ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 18 shows the results of the antiseptic effect measurement experiment.

Table 18 shows the simultaneous inoculation of the fifth strain on the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the sample to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 18> Comparison results of antiseptic effect on the fifth strain of lotion cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁵ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁶ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁵ 4.0 x 10 ⁵ 3.0 x 10 ³ 4.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Comparative Example 2 5.0 x 10 ⁵ 2.0 x 10 ⁴ 6.0 x 10 ³ 3.0 x 10 ³ 2.0 x 10 ³ 3.0 x 10 ³ Comparative Example 3 5.0 x 10 ⁵ 6.0 x 10 ⁴ 4.0 x 10 ³ 4.0 x 10 ³ 7.0 x 10 ³ 6.0 x 10 ³ Comparative Example 4 5.0 x 10 ⁵ 8.0 x 10 ⁴ 8.0 x 10 ³ 7.0 x 10 ³ 3.0 x 10 ³ 7.0 x 10 ³ Comparative Example 5 5.0 x 10 ⁵ 2.0 x 10 ⁴ 5.0 x 10 ³ 5.0 x 10 ³ 8.0 x 10 ³ 1.0 x 10 ³ Comparative Example 6 5.0 x 10 ⁵ 6.0 x 10 ⁴ 3.0 x 10 ³ 6.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Preservative Free 5.0 x 10 ⁵ 6.0 x 10 ⁶ 5.0 x 10 ⁶ 6.0 x 10 ⁵ 8.0 x 10 ⁵ 7.0 x 10 ⁵

In Table 18, the preservative function of the natural preservative composition of the present invention for the lotion cosmetics was much superior to the conventional synthetic preservatives, comparative examples, and no preservatives.

Experimental Example 19

The preservative effect measurement experiments were performed for the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the essence cosmetics of conventional synthetic preservatives.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to the essence cosmetics, respectively, to form samples, and asperfillus niger ATCC90906 5.0 x 10 for each sample. ⁵ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 19 shows the results of the antiseptic effect measurement experiment.

Table 19 shows the simultaneous inoculation of the fifth strain on the sample to which the natural antiseptic composition was added, the sample to which the conventional synthetic preservative was added, the samples to which the fermentation broths of Comparative Examples 1 to 6 were added, and the sample to which the preservative was not added. At the same time, the live viable cell count was simultaneously measured.

<Table 19> Comparison results of antiseptic effect on the sixth strain of essence cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁵ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁶ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁵ 4.0 x 10 ⁵ 3.0 x 10 ³ 4.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Comparative Example 2 5.0 x 10 ⁵ 2.0 x 10 ⁴ 6.0 x 10 ³ 3.0 x 10 ³ 2.0 x 10 ³ 3.0 x 10 ³ Comparative Example 3 5.0 x 10 ⁵ 6.0 x 10 ⁴ 4.0 x 10 ³ 4.0 x 10 ³ 7.0 x 10 ³ 6.0 x 10 ³ Comparative Example 4 5.0 x 10 ⁵ 8.0 x 10 ⁴ 8.0 x 10 ³ 7.0 x 10 ³ 3.0 x 10 ³ 7.0 x 10 ³ Comparative Example 5 5.0 x 10 ⁵ 2.0 x 10 ⁴ 5.0 x 10 ³ 5.0 x 10 ³ 8.0 x 10 ³ 1.0 x 10 ³ Comparative Example 6 5.0 x 10 ⁵ 6.0 x 10 ⁴ 3.0 x 10 ³ 6.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Preservative Free 5.0 x 10 ⁵ 6.0 x 10 ⁶ 5.0 x 10 ⁶ 6.0 x 10 ⁵ 8.0 x 10 ⁵ 7.0 x 10 ⁵

In Table 19, the preservative function of the natural preservative composition of the present invention for the essence cosmetics was much superior to the conventional synthetic preservatives, comparative examples, no preservatives.

Experimental Example 20

The preservative effect measurement experiments were performed on the natural preservative composition prepared according to the above example, the fermentation broth prepared according to Comparative Examples 1 to 6, and the cream cosmetic of the conventional synthetic preservative.

The antiseptic effect measurement experiment was performed by adding conventional synthetic preservatives, fermentation broths of Comparative Examples 1 to 6, and natural preservative compositions of Examples to cream cosmetics to form samples, and asperfillus niger ATCC90906 5.0 x 10 for each sample. ⁵ / g was inoculated and the number of viable cells were measured at a certain time point for 4 weeks to compare the antiseptic effect.

Table 20 shows the results of the antiseptic effect measurement experiment.

Table 20 simultaneously inoculates the fifth strain on the sample to which the natural antiseptic composition is added, the sample to which the conventional synthetic preservative is added, the samples to which the fermentation broths of Comparative Examples 1 to 6 are added, and the sample to which the preservative is not added. At the same time, the live viable cell count was simultaneously measured.

<Table 20> Experimental results of antiseptic effect on 5 strains of cream cosmetics

division Inoculation day 3 days 1 week 2 weeks 3 weeks 4 weeks Example 5.0 x 10 ⁵ 4.0 x 10 ³ 10 0 0 0 Synthetic preservative 5.0 x 10 ⁶ 3.0 x 10 ³ 30 0 0 0 Comparative Example 1 5.0 x 10 ⁵ 4.0 x 10 ⁵ 3.0 x 10 ³ 4.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Comparative Example 2 5.0 x 10 ⁵ 2.0 x 10 ⁴ 6.0 x 10 ³ 3.0 x 10 ³ 2.0 x 10 ³ 3.0 x 10 ³ Comparative Example 3 5.0 x 10 ⁵ 6.0 x 10 ⁴ 4.0 x 10 ³ 4.0 x 10 ³ 7.0 x 10 ³ 6.0 x 10 ³ Comparative Example 4 5.0 x 10 ⁵ 8.0 x 10 ⁴ 8.0 x 10 ³ 7.0 x 10 ³ 3.0 x 10 ³ 7.0 x 10 ³ Comparative Example 5 5.0 x 10 ⁵ 2.0 x 10 ⁴ 5.0 x 10 ³ 5.0 x 10 ³ 8.0 x 10 ³ 1.0 x 10 ³ Comparative Example 6 5.0 x 10 ⁵ 6.0 x 10 ⁴ 3.0 x 10 ³ 6.0 x 10 ³ 5.0 x 10 ³ 7.0 x 10 ³ Preservative Free 5.0 x 10 ⁵ 6.0 x 10 ⁶ 5.0 x 10 ⁶ 6.0 x 10 ⁵ 8.0 x 10 ⁵ 7.0 x 10 ⁵

In Table 20, the antiseptic function of the natural preservative composition of the present invention on the cream cosmetics was much superior to conventional synthetic preservatives, comparative examples, and no preservatives.

In conclusion, the results of multiple preservative effect measurement experiments, including the above experimental examples, proved that the natural preservative composition prepared by the present invention has the same level of preservative function as synthetic preservatives.

In addition, according to the experimental results of the measurement of the antiseptic effect, the natural preservative composition prepared according to the present invention is synergistic interaction between each of the extracts only when the golden extract, bark skin extract, and grapefruit seed extract are all included It has been shown to provide excellent antiseptic function.

By adding a certain amount of the natural preservative composition prepared as described above to the cosmetic, or by adding a certain amount of the pharmaceutical composition of the natural preservative composition to the cosmetic, it provides an excellent antiseptic function to the cosmetic without side effects.

The natural preservative composition prepared according to the present invention is very suitable for preservative treatment of cosmetics in direct contact with the human body because it is made of only natural materials and has no side effects on the human body.

In addition, the natural preservative composition prepared according to the present invention is excellent in the antiseptic effect on cosmetics susceptible to deterioration because the raw material golden, bark skin, grapefruit seeds synergistically exert each other to express excellent antiseptic function.

Although the above has been described with reference to a preferred embodiment of the present invention, those skilled in the art will be able to variously modify and change the present invention without departing from the spirit and scope of the invention as set forth in the claims below. It will be appreciated.

Claims (4)

Forming a golden extract, bark extract, grapefruit seed extract; Mixing 10 to 40 parts by weight of the golden extract, 10 to 30 parts by weight of bark extract, and 15 to 40 parts by weight of grapefruit seed extract to form a mixture including all of the golden extract, the bark extract, and the grapefruit seed extract; Fermenting the mixture at room temperature for 5 to 7 days to form a fermentation composition comprising a liquid phase and a solid content; Recovering the vegetable fermentation broth by solid / liquid separation of the fermentation composition; And Method of producing a natural preservative composition comprising the step of aging the vegetable fermentation broth at room temperature for 5 to 7 days. The method of claim 1, wherein the forming of the golden extract Pulverizing the roots of gold to form a gold pulverization; And The method of producing a natural preservative composition comprising the step of obtaining the liquid golden extract by pressing the golden pulverized. According to claim 1, wherein the step of forming the extract Pulverizing the shell of the root of the peony to form a pulverulent pulverum; And Method of producing a natural preservative composition characterized in that it comprises the step of pressing the pulverulent pulverulent extract to obtain a liquid bark extract. According to claim 1, wherein the step of forming the grapefruit seed extract Pulverizing the grapefruit seeds to form a grapefruit seed powder; And Compressing the grapefruit seed pulverized product to obtain a liquid grapefruit seed extract, the method of producing a natural preservative composition.

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