KR100316347B1 - 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 - Google Patents
대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 Download PDFInfo
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- KR100316347B1 KR100316347B1 KR1019980038061A KR19980038061A KR100316347B1 KR 100316347 B1 KR100316347 B1 KR 100316347B1 KR 1019980038061 A KR1019980038061 A KR 1019980038061A KR 19980038061 A KR19980038061 A KR 19980038061A KR 100316347 B1 KR100316347 B1 KR 100316347B1
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- amino acid
- signal peptide
- growth hormone
- human growth
- variant
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- General Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019980038061A KR100316347B1 (ko) | 1998-09-15 | 1998-09-15 | 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 |
| DK99944889T DK1114062T3 (da) | 1998-09-15 | 1999-09-15 | Modificeret E. coli-enterotoksin II-signalpeptid og en mikroorganisme, som eksprimerer et fusionsprotein af peptidet og et heterologt protein |
| US09/786,569 US6605697B1 (en) | 1998-09-15 | 1999-09-15 | Modified E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of a said peptide and a heterologous protein |
| JP2000570199A JP3701201B2 (ja) | 1998-09-15 | 1999-09-15 | 大腸菌エンテロトキシンｉｉシグナルペプチド変形体およびその変形体と異種タンパク質の融合タンパク質を発現する微生物 |
| DE69929805T DE69929805T2 (de) | 1998-09-15 | 1999-09-15 | Modifiziertes e.coli enterotoxin ii signalpeptid sowie ein mikroorganismus der ein fusionsprotein sowie ein heterologes protein exprimiert |
| NZ510385A NZ510385A (en) | 1998-09-15 | 1999-09-15 | E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of the MST peptide and a heterologous protein, such as human growth hormone |
| AT99944889T ATE317398T1 (de) | 1998-09-15 | 1999-09-15 | Modifiziertes e.coli enterotoxin ii signalpeptid sowie ein mikroorganismus der ein fusionsprotein sowie ein heterologes protein exprimiert |
| PCT/KR1999/000547 WO2000015661A1 (en) | 1998-09-15 | 1999-09-15 | Modified e. coli enterotoxin ii signal peptide and a microorganism expressing a fusion protein of said peptide and a heterologous protein |
| CNB99810924XA CN1144815C (zh) | 1998-09-15 | 1999-09-15 | 修饰的大肠杆菌内毒素ⅱ信号肽以及表达该肽与异源蛋白质的融合蛋白的微生物 |
| RU2001110120/04A RU2198179C2 (ru) | 1998-09-15 | 1999-09-15 | Модифицированный сигнальный пептид энтеротоксина - ii e. coli и микроорганизм, экспрессирующий белок слияния упомянутого пептида с гетерологичным белком |
| EP99944889A EP1114062B1 (de) | 1998-09-15 | 1999-09-15 | Modifiziertes e.coli enterotoxin ii signalpeptid sowie ein mikroorganismus der ein fusionsprotein sowie ein heterologes protein exprimiert |
| AU57618/99A AU754435B2 (en) | 1998-09-15 | 1999-09-15 | Modified E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of said peptide and a heterologous protein |
| CA002342537A CA2342537C (en) | 1998-09-15 | 1999-09-15 | Modified e. coli enterotoxin ii signal peptide and a microorganism expressing a fusion protein of said peptide and a heterologous protein |
| BRPI9913698A BRPI9913698B1 (pt) | 1998-09-15 | 1999-09-15 | peptídeo sinalizador de enterotoxina ii de e. coli modificado e um microorganismo expressando uma proteína de fusão do peptídeo e uma proteína heteróloga |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019980038061A KR100316347B1 (ko) | 1998-09-15 | 1998-09-15 | 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20000019788A KR20000019788A (ko) | 2000-04-15 |
| KR100316347B1 true KR100316347B1 (ko) | 2002-08-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019980038061A KR100316347B1 (ko) | 1998-09-15 | 1998-09-15 | 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6605697B1 (de) |
| EP (1) | EP1114062B1 (de) |
| JP (1) | JP3701201B2 (de) |
| KR (1) | KR100316347B1 (de) |
| CN (1) | CN1144815C (de) |
| AT (1) | ATE317398T1 (de) |
| AU (1) | AU754435B2 (de) |
| BR (1) | BRPI9913698B1 (de) |
| CA (1) | CA2342537C (de) |
| DE (1) | DE69929805T2 (de) |
| DK (1) | DK1114062T3 (de) |
| NZ (1) | NZ510385A (de) |
| RU (1) | RU2198179C2 (de) |
| WO (1) | WO2000015661A1 (de) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7736653B2 (en) | 2003-11-13 | 2010-06-15 | Hanmi Pharm. Co., Ltd | Pharmaceutical composition comprising an immunoglobulin Fc region as a carrier |
| US8110665B2 (en) | 2003-11-13 | 2012-02-07 | Hanmi Holdings Co., Ltd. | Pharmaceutical composition comprising an immunoglobulin FC region as a carrier |
| WO2012057529A2 (en) | 2010-10-29 | 2012-05-03 | Hanmi Holdings Co., Ltd. | Method for purifying human granulocyte-colony stimulating factor from recombinant e. coli |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6242177B1 (en) * | 1995-03-01 | 2001-06-05 | Genentech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
| KR100356140B1 (ko) * | 1999-07-08 | 2002-10-19 | 한미약품공업 주식회사 | 인간 과립구 콜로니 자극인자 변이체 및 이의 생산 방법 |
| KR100360594B1 (ko) * | 2000-01-19 | 2002-11-13 | 한미약품공업 주식회사 | 인간 인터페론 알파의 발현 분비벡터 및 이를 이용한인터페론 알파의 생산 방법 |
| KR100570422B1 (ko) * | 2003-10-16 | 2006-04-11 | 한미약품 주식회사 | 대장균 분비서열을 이용하여 항체 단편을 분비·생산하는 발현벡터 및 이를 이용하여 항체 단편을 대량 생산하는 방법 |
| EP2049148B1 (de) | 2006-07-06 | 2016-09-28 | Daewoong Co., Ltd. | Stabile flüssige formulierung von humanem wachstumshormon |
| US20080124355A1 (en) | 2006-09-22 | 2008-05-29 | David Gordon Bermudes | Live bacterial vaccines for viral infection prophylaxis or treatment |
| US8241623B1 (en) | 2009-02-09 | 2012-08-14 | David Bermudes | Protease sensitivity expression system |
| US9597379B1 (en) | 2010-02-09 | 2017-03-21 | David Gordon Bermudes | Protease inhibitor combination with therapeutic proteins including antibodies |
| US8524220B1 (en) | 2010-02-09 | 2013-09-03 | David Gordon Bermudes | Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria |
| US8771669B1 (en) | 2010-02-09 | 2014-07-08 | David Gordon Bermudes | Immunization and/or treatment of parasites and infectious agents by live bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| ATE78515T1 (de) * | 1984-10-05 | 1992-08-15 | Genentech Inc | Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. |
| JPS63230089A (ja) * | 1987-03-18 | 1988-09-26 | Takeda Chem Ind Ltd | 分泌発現による蛋白質の製造法 |
| JPH0479898A (ja) * | 1990-07-23 | 1992-03-13 | Kitasato Inst:The | コレラ菌及び毒素原性大腸菌を同時検出するためのｄｎａ及びｒｎａプローブとそれらを用いたコレラ菌及び毒素原性大腸菌の検出方法 |
| EP0626448A3 (de) * | 1993-05-26 | 1998-01-14 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verfahren zur Herstellung und Reinigung von alpha-Interferon |
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- 1999-09-15 JP JP2000570199A patent/JP3701201B2/ja not_active Expired - Lifetime
- 1999-09-15 RU RU2001110120/04A patent/RU2198179C2/ru active
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7736653B2 (en) | 2003-11-13 | 2010-06-15 | Hanmi Pharm. Co., Ltd | Pharmaceutical composition comprising an immunoglobulin Fc region as a carrier |
| US7737260B2 (en) | 2003-11-13 | 2010-06-15 | Hanmi Pharm. Co., Ltd | Protein complex using an immunoglobulin fragment and method for the preparation thereof |
| US8029789B2 (en) | 2003-11-13 | 2011-10-04 | Hanmi Holdings Co., Ltd. | Method for the mass production of immunoglobulin constant region |
| US8110665B2 (en) | 2003-11-13 | 2012-02-07 | Hanmi Holdings Co., Ltd. | Pharmaceutical composition comprising an immunoglobulin FC region as a carrier |
| US8822650B2 (en) | 2003-11-13 | 2014-09-02 | Hanmi Science Co., Ltd | Method for the mass production of immunoglobulin constant region |
| US8846874B2 (en) | 2003-11-13 | 2014-09-30 | Hanmi Science Co., Ltd | IgG Fc fragment for a drug carrier and method for the preparation thereof |
| US9750820B2 (en) | 2003-11-13 | 2017-09-05 | Hanmi Science Co., Ltd. | IgG Fc fragment for a drug carrier and method for the preparation thereof |
| US10071166B2 (en) | 2003-11-13 | 2018-09-11 | Hanmi Science Co., Ltd. | Protein complex using an immunoglobulin fragment and method for the preparation thereof |
| US10272159B2 (en) | 2003-11-13 | 2019-04-30 | Hanmi Science Co., Ltd. | IgG Fc fragment for a drug carrier and method for the preparation thereof |
| US11058776B2 (en) | 2003-11-13 | 2021-07-13 | Hanmi Science Co., Ltd. | IgG Fc fragment for a drug carrier and method for the preparation thereof |
| WO2012057529A2 (en) | 2010-10-29 | 2012-05-03 | Hanmi Holdings Co., Ltd. | Method for purifying human granulocyte-colony stimulating factor from recombinant e. coli |
Also Published As
| Publication number | Publication date |
|---|---|
| DK1114062T3 (da) | 2006-06-06 |
| DE69929805D1 (de) | 2006-04-20 |
| CA2342537C (en) | 2008-07-08 |
| NZ510385A (en) | 2002-12-20 |
| US6605697B1 (en) | 2003-08-12 |
| AU5761899A (en) | 2000-04-03 |
| EP1114062A1 (de) | 2001-07-11 |
| JP2002538765A (ja) | 2002-11-19 |
| CN1144815C (zh) | 2004-04-07 |
| DE69929805T2 (de) | 2006-10-26 |
| RU2198179C2 (ru) | 2003-02-10 |
| BRPI9913698B1 (pt) | 2016-06-21 |
| BR9913698A (pt) | 2002-01-29 |
| EP1114062B1 (de) | 2006-02-08 |
| CA2342537A1 (en) | 2000-03-23 |
| KR20000019788A (ko) | 2000-04-15 |
| ATE317398T1 (de) | 2006-02-15 |
| CN1318070A (zh) | 2001-10-17 |
| WO2000015661A1 (en) | 2000-03-23 |
| JP3701201B2 (ja) | 2005-09-28 |
| AU754435B2 (en) | 2002-11-14 |
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