JPWO2020081415A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2020081415A5 JPWO2020081415A5 JP2021545269A JP2021545269A JPWO2020081415A5 JP WO2020081415 A5 JPWO2020081415 A5 JP WO2020081415A5 JP 2021545269 A JP2021545269 A JP 2021545269A JP 2021545269 A JP2021545269 A JP 2021545269A JP WO2020081415 A5 JPWO2020081415 A5 JP WO2020081415A5
- Authority
- JP
- Japan
- Prior art keywords
- aav
- composition
- target cells
- hours
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 claims description 86
- 239000000523 sample Substances 0.000 claims description 28
- 239000002245 particle Substances 0.000 claims description 22
- 108020004707 nucleic acids Proteins 0.000 claims description 19
- 150000007523 nucleic acids Chemical class 0.000 claims description 19
- 229920000023 polynucleotide Polymers 0.000 claims description 16
- 239000002157 polynucleotide Substances 0.000 claims description 16
- 230000003612 virological Effects 0.000 claims description 16
- 238000003752 polymerase chain reaction Methods 0.000 claims description 12
- 241001068295 Replication defective viruses Species 0.000 claims description 11
- 238000010790 dilution Methods 0.000 claims description 11
- 241000700605 Viruses Species 0.000 claims description 9
- 229920001850 Nucleic acid sequence Polymers 0.000 claims description 7
- 102000004040 Capsid Proteins Human genes 0.000 claims description 6
- 108090000565 Capsid Proteins Proteins 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 5
- 230000005017 genetic modification Effects 0.000 claims description 4
- 235000013617 genetically modified food Nutrition 0.000 claims description 4
- 241000702423 Adeno-associated virus - 2 Species 0.000 claims description 3
- 102100017074 DNAJB7 Human genes 0.000 claims description 3
- 101710007744 DNAJB7 Proteins 0.000 claims description 3
- 241000713666 Lentivirus Species 0.000 claims description 3
- 241000700618 Vaccinia virus Species 0.000 claims description 3
- 239000012472 biological sample Substances 0.000 claims description 3
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims description 3
- 241000701161 unidentified adenovirus Species 0.000 claims description 3
- 238000010353 genetic engineering Methods 0.000 claims description 2
- 230000001809 detectable Effects 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862745859P | 2018-10-15 | 2018-10-15 | |
| US62/745,859 | 2018-10-15 | ||
| PCT/US2019/056042 WO2020081415A1 (en) | 2018-10-15 | 2019-10-14 | Method for measuring the infectivity of replication defective viral vectors and viruses |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2022514112A JP2022514112A (ja) | 2022-02-09 |
| JPWO2020081415A5 true JPWO2020081415A5 (ko) | 2022-10-21 |
Family
ID=68393096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021545269A Pending JP2022514112A (ja) | 2018-10-15 | 2019-10-14 | 複製欠損型ウイルスベクター及びウイルスの感染性を測定するための方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20210324483A1 (ko) |
| EP (1) | EP3867412A1 (ko) |
| JP (1) | JP2022514112A (ko) |
| KR (1) | KR20210075994A (ko) |
| CN (1) | CN112912518A (ko) |
| AU (1) | AU2019362833A1 (ko) |
| CA (1) | CA3115524A1 (ko) |
| IL (1) | IL282195A (ko) |
| WO (1) | WO2020081415A1 (ko) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115197967B (zh) * | 2021-04-08 | 2023-09-15 | 广州派真生物技术有限公司 | 制备重组腺相关病毒的辅助质粒及其应用 |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5658785A (en) * | 1994-06-06 | 1997-08-19 | Children's Hospital, Inc. | Adeno-associated virus materials and methods |
| US7198784B2 (en) | 1996-10-17 | 2007-04-03 | Oxford Biomedica (Uk) Limited | Retroviral vectors |
| US6995006B2 (en) | 1997-09-05 | 2006-02-07 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
| US8715940B2 (en) | 1999-04-06 | 2014-05-06 | Wisconsin Alumni Research Foundation | Method of making recombinant influenza virus |
| EP2369002A1 (en) | 1999-08-09 | 2011-09-28 | Targeted Genetics Corporation | Enhancement of expression of a single-stranded, heterologous nucleotide sequence from recombinant viral vectors by designing the sequence such that it forms intrastrand base pairs |
| AU2002305778A1 (en) | 2001-05-31 | 2002-12-09 | The Rockefeller University | Method for generating replication defective viral vectors that are helper free |
| US6723551B2 (en) | 2001-11-09 | 2004-04-20 | The United States Of America As Represented By The Department Of Health And Human Services | Production of adeno-associated virus in insect cells |
| CA2406745C (en) | 2001-11-13 | 2006-01-10 | The Trustees Of The University Of Pennsylvania | A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby |
| DK1453547T3 (en) | 2001-12-17 | 2016-12-05 | Univ Pennsylvania | ADENOASSOCATED VIRUS (AAV) SEROTYPE 8 SEQUENCES, VECTORS CONTAINING THESE AND APPLICATIONS THEREOF |
| US20060286545A1 (en) | 2003-05-23 | 2006-12-21 | Mount Sinai School Of Medicine Of New York University | Viral vectors with improved properties |
| AU2004278684B2 (en) | 2003-09-30 | 2011-05-12 | The Trustees Of The University Of Pennsylvania | Adeno-associated virus (AAV) clades, sequences, vectors containing same, and uses therefor |
| ES2386272T3 (es) * | 2004-09-09 | 2012-08-16 | Novartis Vaccines And Diagnostics Gmbh | Reducción de riesgos iatrogénicos potenciales asociados a las vacunas antigripales |
| US7183969B2 (en) | 2004-12-22 | 2007-02-27 | Raytheon Company | System and technique for calibrating radar arrays |
| CN112029737A (zh) | 2005-04-07 | 2020-12-04 | 宾夕法尼亚大学托管会 | 增强腺相关病毒载体功能的方法 |
| JP4495210B2 (ja) | 2005-06-09 | 2010-06-30 | パナソニック株式会社 | 振幅誤差補償装置及び直交度誤差補償装置 |
| JP2009516510A (ja) * | 2005-11-21 | 2009-04-23 | アンスティテュ パストゥール | 単一の生細胞におけるウイルス遺伝子発現動態のリアルタイム定量分析のためのツール |
| WO2009097129A1 (en) | 2008-01-29 | 2009-08-06 | Applied Genetic Technologies Corporation | Recombinant virus production using mammalian cells in suspension |
| ES2644880T3 (es) | 2008-02-19 | 2017-11-30 | Uniqure Ip B.V. | Optimización de expresión de proteínas Rep y Cap parvovirales en las células de insecto |
| US10087493B2 (en) * | 2008-03-07 | 2018-10-02 | Aptalis Pharma Canada Ulc | Method for detecting infectious parvovirus in pharmaceutical preparations |
| EP2425000B1 (en) | 2009-04-30 | 2019-02-20 | The Trustees Of The University Of Pennsylvania | Compositions for targeting conducting airway cells comprising adeno-associated virus constructs |
| EP2429579A1 (en) | 2009-05-12 | 2012-03-21 | Transgene SA | Immortalized avian cell lines and use thereof |
| US8734809B2 (en) | 2009-05-28 | 2014-05-27 | University Of Massachusetts | AAV's and uses thereof |
| JP2012529892A (ja) * | 2009-06-19 | 2012-11-29 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼーション | ウイルス・アッセイ |
| SI2516629T1 (sl) | 2009-12-21 | 2016-10-28 | The Brigham And Women's Hospital, Inc. | Cepiva proti virusu herpes simplex |
| US9862931B2 (en) | 2010-04-28 | 2018-01-09 | Institut Gustave Roussy | Adenovirus vaccine vectors |
| US8628966B2 (en) | 2010-04-30 | 2014-01-14 | City Of Hope | CD34-derived recombinant adeno-associated vectors for stem cell transduction and systemic therapeutic gene transfer |
| US8927514B2 (en) | 2010-04-30 | 2015-01-06 | City Of Hope | Recombinant adeno-associated vectors for targeted treatment |
| EP2634253B1 (en) | 2010-10-27 | 2016-05-11 | Jichi Medical University | Adeno-associated virus virions for transferring genes into neural cells |
| WO2012109570A1 (en) | 2011-02-10 | 2012-08-16 | The University Of North Carolina At Chapel Hill | Viral vectors with modified transduction profiles and methods of making and using the same |
| SG10201800873WA (en) | 2011-04-22 | 2018-03-28 | Univ California | Adeno-associated virus virions with variant capsid and methods of use thereof |
| US9796973B2 (en) * | 2011-04-28 | 2017-10-24 | Yamaguchi University | Terminator sequence-containing reverse primer for overexpression and linear DNA |
| WO2013029030A1 (en) | 2011-08-24 | 2013-02-28 | The Board Of Trustees Of The Leland Stanford Junior University | New aav capsid proteins for nucleic acid transfer |
| JP6385920B2 (ja) | 2012-05-09 | 2018-09-05 | オレゴン ヘルス アンド サイエンス ユニバーシティー | アデノ随伴ウイルスプラスミド及びベクター |
| CA3200425A1 (en) | 2012-11-16 | 2014-05-22 | Peter ABBINK | Recombinant adenoviruses and use thereof |
| AU2014244167A1 (en) | 2013-03-13 | 2015-10-08 | The Children's Hospital Of Philadelphia | Adeno-associated virus vectors and methods of use thereof |
| KR20220090593A (ko) | 2013-04-20 | 2022-06-29 | 더 리서치 인스티튜트 앳 네이션와이드 칠드런스 하스피탈 | 엑손 2-표적 U7snRNA 폴리뉴클레오티드 작제물의 재조합형 아데노 부속 바이러스 전달 |
| DK3024498T3 (da) | 2013-07-22 | 2020-03-02 | Childrens Hospital Philadelphia | Aav-variant og sammensætninger, fremgangsmåder og anvendelser til genoverførsel til celler, organer og væv |
| EP3564379A1 (en) | 2013-09-13 | 2019-11-06 | California Institute of Technology | Selective recovery |
| CN115093464A (zh) | 2013-10-11 | 2022-09-23 | 马萨诸塞眼科耳科诊所 | 预测祖先病毒序列的方法及其用途 |
| CN105765066B (zh) * | 2013-11-29 | 2018-12-14 | 宝生物工程株式会社 | 用于定量腺伴随病毒的方法 |
| US11230719B2 (en) | 2014-03-26 | 2022-01-25 | Denovo Biopharma Llc | Retroviral vector having immune-stimulating activity |
| WO2015164757A1 (en) | 2014-04-25 | 2015-10-29 | Oregon Health & Science University | Methods of viral neutralizing antibody epitope mapping |
| WO2015191508A1 (en) | 2014-06-09 | 2015-12-17 | Voyager Therapeutics, Inc. | Chimeric capsids |
| EP3198018B1 (en) | 2014-09-24 | 2020-12-09 | City of Hope | Adeno-associated virus vector variants for high efficiency genome editing and methods thereof |
| JP6665466B2 (ja) | 2015-09-26 | 2020-03-13 | 日亜化学工業株式会社 | 半導体発光素子及びその製造方法 |
| WO2017070491A1 (en) | 2015-10-23 | 2017-04-27 | Applied Genetic Technologies Corporation | Ophthalmic formulations |
| MX2018012537A (es) * | 2016-04-15 | 2019-02-25 | Univ Pennsylvania | Terapia de genes para tratar hemofilia a. |
| EP4365311A2 (en) * | 2016-09-07 | 2024-05-08 | St Vincent's Hospital Sydney Limited | Methods of detecting lentivirus |
-
2019
- 2019-10-14 AU AU2019362833A patent/AU2019362833A1/en active Pending
- 2019-10-14 CN CN201980067652.8A patent/CN112912518A/zh active Pending
- 2019-10-14 US US17/285,362 patent/US20210324483A1/en active Pending
- 2019-10-14 EP EP19795740.0A patent/EP3867412A1/en active Pending
- 2019-10-14 WO PCT/US2019/056042 patent/WO2020081415A1/en unknown
- 2019-10-14 JP JP2021545269A patent/JP2022514112A/ja active Pending
- 2019-10-14 KR KR1020217010729A patent/KR20210075994A/ko unknown
- 2019-10-14 CA CA3115524A patent/CA3115524A1/en active Pending
-
2021
- 2021-04-08 IL IL282195A patent/IL282195A/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3943048B2 (ja) | 組織の細胞ｄｎａからの組込みウイルスの直接レスキュー及び増幅の方法 | |
| JP6741345B2 (ja) | パルボウイルスビリオンを含む組成物におけるｄｎａ不純物 | |
| US20030138772A1 (en) | Method of detecting and/or identifying adeno-associated virus (AAV) sequences and isolating novel sequences identified thereby | |
| Bonvicini et al. | Parvovirus b19 DNA CpG dinucleotide methylation and epigenetic regulation of viral expression | |
| CN116411024A (zh) | 用于生产重组腺相关病毒的质粒系统 | |
| JPWO2020081415A5 (ko) | ||
| CN114075610A (zh) | 检测野生型腺相关病毒的通用引物及其应用 | |
| Purohit et al. | Molecular characterization of novel Adeno-associated virus variants infecting human tissues | |
| CN116622908B (zh) | 快速检测野生型腺相关病毒的引物探针、试剂盒及方法和应用 | |
| JP2023511108A (ja) | ウイルス力価を決定する方法 | |
| JP2022514112A (ja) | 複製欠損型ウイルスベクター及びウイルスの感染性を測定するための方法 | |
| US20230295608A1 (en) | Methods for Characterization of Viral Genome Using Base Modifications | |
| JP2023545835A (ja) | 遺伝子療法製剤の効力を測定するためのアッセイ | |
| Schmitt et al. | Genotyping of AAV plasmid stocks: quality control in adeno-associated virus vector production | |
| WO2024123759A1 (en) | Droplet digital pcr assay for determining viral vector genomic titer | |
| KR20190012378A (ko) | 아데노 연관 바이러스의 정량방법 | |
| US20220242917A1 (en) | Compositions and methods for producing adeno-associated viral vectors | |
| AU2021361063A9 (en) | Assay for measuring potency of gene therapy drug product | |
| CN115807064A (zh) | 重组腺相关病毒载体制品中游离和错误包装dna的检测方法 | |
| CN117025843A (zh) | 重组型腺相关病毒制品中复制型腺相关病毒rcAAV8的检测组合物、方法及试剂盒 | |
| JP2004305092A (ja) | ヒトアデノウイルスの定量方法並びにそのためのプライマー及びプローブ | |
| TW202321692A (zh) | 用於測定ａａｖ衣殼比率之液相層析分析法 | |
| Jasani et al. | Molecular Detection of Siman Virus 40 in Human Mesothelioma |