JPWO2020061371A5 - - Google Patents
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- JPWO2020061371A5 JPWO2020061371A5 JP2021516628A JP2021516628A JPWO2020061371A5 JP WO2020061371 A5 JPWO2020061371 A5 JP WO2020061371A5 JP 2021516628 A JP2021516628 A JP 2021516628A JP 2021516628 A JP2021516628 A JP 2021516628A JP WO2020061371 A5 JPWO2020061371 A5 JP WO2020061371A5
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- Prior art keywords
- cells
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- pluripotent stem
- inhibitor
- stem cells
- Prior art date
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- 210000004027 cell Anatomy 0.000 claims description 112
- 238000000034 method Methods 0.000 claims description 75
- 239000003112 inhibitor Substances 0.000 claims description 37
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 29
- 230000011664 signaling Effects 0.000 claims description 23
- 102000004887 Transforming Growth Factor beta Human genes 0.000 claims description 21
- 108090001012 Transforming Growth Factor beta Proteins 0.000 claims description 21
- 102000007350 Bone Morphogenetic Proteins Human genes 0.000 claims description 20
- 108010007726 Bone Morphogenetic Proteins Proteins 0.000 claims description 20
- 102000001393 Platelet-Derived Growth Factor alpha Receptor Human genes 0.000 claims description 20
- 108010068588 Platelet-Derived Growth Factor alpha Receptor Proteins 0.000 claims description 20
- 229940112869 bone morphogenetic protein Drugs 0.000 claims description 20
- 239000000725 suspension Substances 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 17
- 230000002518 glial effect Effects 0.000 claims description 17
- 210000000130 stem cell Anatomy 0.000 claims description 16
- 108010059616 Activins Proteins 0.000 claims description 15
- 102100026818 Inhibin beta E chain Human genes 0.000 claims description 15
- 239000000488 activin Substances 0.000 claims description 15
- 102000013380 Smoothened Receptor Human genes 0.000 claims description 12
- 108010090739 Smoothened Receptor Proteins 0.000 claims description 12
- 239000000556 agonist Substances 0.000 claims description 10
- 102100037506 Paired box protein Pax-6 Human genes 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 102100034111 Activin receptor type-1 Human genes 0.000 claims description 8
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 claims description 8
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- 101000799140 Homo sapiens Activin receptor type-1 Proteins 0.000 claims description 8
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- 108010011702 Transforming Growth Factor-beta Type I Receptor Proteins 0.000 claims description 8
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- 102000004619 Nodal Signaling Ligands Human genes 0.000 claims description 6
- 108010017886 Nodal Signaling Ligands Proteins 0.000 claims description 6
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims description 6
- XHBVYDAKJHETMP-UHFFFAOYSA-N dorsomorphin Chemical compound C=1C=C(C2=CN3N=CC(=C3N=C2)C=2C=CN=CC=2)C=CC=1OCCN1CCCCC1 XHBVYDAKJHETMP-UHFFFAOYSA-N 0.000 claims description 6
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- FYBHCRQFSFYWPY-UHFFFAOYSA-N purmorphamine Chemical compound C1CCCCC1N1C2=NC(OC=3C4=CC=CC=C4C=CC=3)=NC(NC=3C=CC(=CC=3)N3CCOCC3)=C2N=C1 FYBHCRQFSFYWPY-UHFFFAOYSA-N 0.000 claims description 6
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- 229960001727 tretinoin Drugs 0.000 claims description 6
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims description 6
- CJLMANFTWLNAKC-UHFFFAOYSA-N 3-[6-amino-5-(3,4,5-trimethoxyphenyl)pyridin-3-yl]phenol Chemical compound COC1=C(OC)C(OC)=CC(C=2C(=NC=C(C=2)C=2C=C(O)C=CC=2)N)=C1 CJLMANFTWLNAKC-UHFFFAOYSA-N 0.000 claims description 4
- CDOVNWNANFFLFJ-UHFFFAOYSA-N 4-[6-[4-(1-piperazinyl)phenyl]-3-pyrazolo[1,5-a]pyrimidinyl]quinoline Chemical compound C1CNCCN1C1=CC=C(C2=CN3N=CC(=C3N=C2)C=2C3=CC=CC=C3N=CC=2)C=C1 CDOVNWNANFFLFJ-UHFFFAOYSA-N 0.000 claims description 4
- JMIFGARJSWXZSH-UHFFFAOYSA-N DMH1 Chemical compound C1=CC(OC(C)C)=CC=C1C1=CN2N=CC(C=3C4=CC=CC=C4N=CC=3)=C2N=C1 JMIFGARJSWXZSH-UHFFFAOYSA-N 0.000 claims description 4
- 102000002140 Fatty Acid-Binding Protein 7 Human genes 0.000 claims description 4
- 108010001387 Fatty Acid-Binding Protein 7 Proteins 0.000 claims description 4
- 101000843449 Homo sapiens Transcription factor HES-5 Proteins 0.000 claims description 4
- 101000910748 Homo sapiens Voltage-dependent calcium channel gamma-4 subunit Proteins 0.000 claims description 4
- -1 ML3467 Chemical compound 0.000 claims description 4
- 108700003766 Promyelocytic Leukemia Zinc Finger Proteins 0.000 claims description 4
- 102100030853 Transcription factor HES-5 Human genes 0.000 claims description 4
- 102100024143 Voltage-dependent calcium channel gamma-4 subunit Human genes 0.000 claims description 4
- 102100040314 Zinc finger and BTB domain-containing protein 16 Human genes 0.000 claims description 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 4
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 4
- 102000045246 noggin Human genes 0.000 claims description 4
- 108700007229 noggin Proteins 0.000 claims description 4
- 238000007747 plating Methods 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 238000004114 suspension culture Methods 0.000 claims description 4
- 108091008606 PDGF receptors Proteins 0.000 claims description 3
- 102000011653 Platelet-Derived Growth Factor Receptors Human genes 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 150000002270 gangliosides Chemical class 0.000 claims description 3
- 108700002956 human laminin 11 Proteins 0.000 claims description 3
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- 230000001537 neural effect Effects 0.000 claims description 3
- 101100107081 Danio rerio zbtb16a gene Proteins 0.000 claims description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 2
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- 101000601647 Homo sapiens Paired box protein Pax-6 Proteins 0.000 claims description 2
- 101100377226 Homo sapiens ZBTB16 gene Proteins 0.000 claims description 2
- 108010085895 Laminin Proteins 0.000 claims description 2
- 102000007547 Laminin Human genes 0.000 claims description 2
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 claims description 2
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 claims description 2
- 108010031318 Vitronectin Proteins 0.000 claims description 2
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- 210000004248 oligodendroglia Anatomy 0.000 claims description 2
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- 108010032788 PAX6 Transcription Factor Proteins 0.000 claims 4
- 230000001464 adherent effect Effects 0.000 claims 1
- ZZVUWRFHKOJYTH-UHFFFAOYSA-N diphenhydramine Chemical compound C=1C=CC=CC=1C(OCCN(C)C)C1=CC=CC=C1 ZZVUWRFHKOJYTH-UHFFFAOYSA-N 0.000 claims 1
- 108010046591 human laminin-511 Proteins 0.000 claims 1
- 102000007573 human laminin-511 Human genes 0.000 claims 1
- 102100029233 Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase Human genes 0.000 description 1
- 101710115567 Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase Proteins 0.000 description 1
- 108010038862 laminin 10 Proteins 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024169082A JP2024178446A (ja) | 2018-09-19 | 2024-09-27 | 動的懸濁培養において多能性幹細胞を分化させるための方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862733621P | 2018-09-19 | 2018-09-19 | |
| US62/733,621 | 2018-09-19 | ||
| PCT/US2019/052015 WO2020061371A2 (en) | 2018-09-19 | 2019-09-19 | Methods for differentiating pluripotent stem cells in dynamic suspension culture |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024169082A Division JP2024178446A (ja) | 2018-09-19 | 2024-09-27 | 動的懸濁培養において多能性幹細胞を分化させるための方法 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2022501049A JP2022501049A (ja) | 2022-01-06 |
| JPWO2020061371A5 true JPWO2020061371A5 (https=) | 2022-09-20 |
| JP2022501049A5 JP2022501049A5 (https=) | 2022-09-20 |
| JP7569089B2 JP7569089B2 (ja) | 2024-10-17 |
Family
ID=69772854
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021516628A Active JP7569089B2 (ja) | 2018-09-19 | 2019-09-19 | 動的懸濁培養において多能性幹細胞を分化させるための方法 |
| JP2024169082A Pending JP2024178446A (ja) | 2018-09-19 | 2024-09-27 | 動的懸濁培養において多能性幹細胞を分化させるための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024169082A Pending JP2024178446A (ja) | 2018-09-19 | 2024-09-27 | 動的懸濁培養において多能性幹細胞を分化させるための方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US20200087622A1 (https=) |
| EP (1) | EP3853345A4 (https=) |
| JP (2) | JP7569089B2 (https=) |
| KR (1) | KR20210068050A (https=) |
| CN (2) | CN120060140A (https=) |
| AU (1) | AU2019342741B2 (https=) |
| CA (1) | CA3113640A1 (https=) |
| IL (1) | IL281628B2 (https=) |
| WO (1) | WO2020061371A2 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116121189A (zh) | 2016-03-30 | 2023-05-16 | 阿斯特利亚斯生物治疗股份公司 | 少突胶质细胞祖细胞组合物 |
| IL281628B2 (en) * | 2018-09-19 | 2025-06-01 | Lineage Cell Therapeutics Inc | Methods for differentiating pluripotent stem cells in dynamic suspension culture |
| WO2020154533A1 (en) | 2019-01-23 | 2020-07-30 | Asterias Biotherapeutics, Inc. | Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells |
| CN114375328B (zh) * | 2019-09-06 | 2024-12-27 | 学校法人庆应义塾 | 包含胶质前体细胞的细胞聚集体的制备方法 |
| CN112640887B (zh) * | 2020-12-25 | 2022-05-13 | 武汉睿健医药科技有限公司 | 一种神经干细胞冻存液及其应用 |
| WO2022134031A1 (zh) * | 2020-12-25 | 2022-06-30 | 武汉睿健医药科技有限公司 | 一种光感受器神经元细胞的化学诱导方法 |
| IL305737A (en) * | 2021-03-10 | 2023-11-01 | Lineage Cell Therapeutics Inc | Methods for generating oligodendrocyte progenitor cells and their use |
| CN117321191A (zh) | 2021-03-30 | 2023-12-29 | 特雷尔赫德生物系统股份有限公司 | 用于生成少突胶质细胞祖细胞的方法和组合物 |
| EP4419685A1 (en) * | 2021-10-20 | 2024-08-28 | University of Rochester | Compositions and methods for treating myelin deficiency by rejuvenating glial progenitor cells |
| CA3256595A1 (en) * | 2022-05-24 | 2023-11-30 | Bluerock Therapeutics Lp | METHODS FOR MANUFACTURED OLIGODENDROCYTE PROGENITOR CELLS |
| CN119654404A (zh) * | 2022-08-08 | 2025-03-18 | 特雷尔赫德生物系统股份有限公司 | 产生少突胶质祖细胞的方法和组合物 |
| WO2025171344A1 (en) * | 2024-02-09 | 2025-08-14 | Cedars-Sinai Medical Center | Expansion, harvest and cryopreservation of induced pluripotent stem cells with cgmps |
| CN120081923B (zh) * | 2025-04-28 | 2025-08-01 | 四川华德生物工程有限公司 | 一种动物源表皮生长因子的智能优化方法和系统 |
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| WO1998048001A1 (en) | 1997-04-24 | 1998-10-29 | California Institute Of Technology | Methods for differentiating neural stem cells |
| US6361996B1 (en) | 1997-05-07 | 2002-03-26 | University Of Utah Research Foundation | Neuroepithelial stem cells and glial-restricted intermediate precursors |
| CA2388736A1 (en) | 1999-10-22 | 2001-04-26 | Washington University | Oligodendrocyte cell cultures and methods for their preparation and use |
| US6280718B1 (en) | 1999-11-08 | 2001-08-28 | Wisconsin Alumni Reasearch Foundation | Hematopoietic differentiation of human pluripotent embryonic stem cells |
| CA2403000C (en) | 2000-03-14 | 2015-06-23 | Es Cell International Pte Ltd | Embryonic stem cells and neural progenitor cells derived therefrom |
| JP5943533B2 (ja) | 2000-05-17 | 2016-07-06 | アステリアス バイオセラピューティクス インコーポレイテッド | 神経前駆細胞の集団 |
| AU2003211063A1 (en) | 2002-02-15 | 2003-09-09 | Cornell Research Foundation, Inc. | Myelination of congenitally dysmyelinated forebrains using oligodendrocyte progenitor cells |
| US20050101014A1 (en) | 2002-07-11 | 2005-05-12 | Keirstead Hans S. | Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury |
| US7285415B2 (en) | 2002-07-11 | 2007-10-23 | The Regents Of The University Of California | Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury |
| US7390659B2 (en) | 2002-07-16 | 2008-06-24 | The Trustees Of Columbia University In The City Of New York | Methods for inducing differentiation of embryonic stem cells and uses thereof |
| EP2399990B1 (en) | 2003-06-27 | 2015-07-22 | DePuy Synthes Products, Inc. | Cells derived from post-partum umbilical cord for use in treatment of disease of the heart and circulatory system |
| AR046076A1 (es) | 2003-07-18 | 2005-11-23 | Otsuka Pharma Co Ltd | Procedimiento para obtener una poblacion homogenea de celulas precursoras de oligodendrocitos y poblacion obtenida |
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| KR100941036B1 (ko) | 2007-10-05 | 2010-02-05 | 고려대학교 산학협력단 | 인간 배아줄기세포로부터 척수신경계 희소돌기 아교세포생산을 위한 삼단계 분화기법 |
| EP2217252A2 (en) | 2007-10-29 | 2010-08-18 | Hadasit Medical Research Services & Development Limited | Human stem cell-derived neural precursors for treatment of autoimmune diseases of the central nervous system |
| US8227247B2 (en) | 2007-12-20 | 2012-07-24 | Wisconsin Alumni Research Foundation | Method of generating myelinating oligodendrocytes |
| CA2714010C (en) | 2008-01-30 | 2020-06-16 | Corning Incorporated | Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells |
| KR20110011658A (ko) | 2008-05-08 | 2011-02-08 | 유니버시티 오브 로체스터 | 최적화된 세포 제제에 의한 수초 질환의 치료 |
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| WO2012021818A2 (en) | 2010-08-13 | 2012-02-16 | Georgetown University | Ggf2 and methods of use |
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| EP2897670A4 (en) | 2012-09-24 | 2016-05-25 | Univ California | INJECTION SYSTEM FOR BACKMARK WITH PULSATION SUPPRESSION |
| EP2954046B1 (en) | 2013-02-06 | 2025-10-15 | University Of Rochester | Induced pluripotent cell-derived oligodendrocyte progenitor cells for the treatment of myelin disorders |
| US20140248696A1 (en) | 2013-03-01 | 2014-09-04 | Wisconsin Alumni Research Foundation | Methods of maintaining, expanding, and diffrentiating neuronal subtype specific progenitors |
| US10160950B2 (en) | 2013-03-01 | 2018-12-25 | Wisconsin Alumni Research Foundation | Methods of maintaining, expanding and differentiating neuronal subtype specific progenitors |
| KR20230058556A (ko) * | 2013-03-15 | 2023-05-03 | 아스텔라스 인스티튜트 포 리제너러티브 메디슨 | 만능 줄기 세포로부터 제조된 광수용기 및 광수용기 전구체 |
| WO2015088625A2 (en) | 2013-09-25 | 2015-06-18 | Case Western Reserve University | Compounds and methods of promoting myelination |
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| KR102585909B1 (ko) | 2014-05-22 | 2023-10-05 | 뉴욕 스템 셀 파운데이션, 인코포레이티드 | 다능성 줄기 세포로부터 유래된 기능성 희소돌기아교세포 및 이의 제조 및 사용 방법 |
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| WO2016103269A1 (en) * | 2014-12-23 | 2016-06-30 | Ramot At Tel-Aviv University Ltd. | Populations of neural progenitor cells and methods of producing and using same |
| US10286009B2 (en) | 2015-05-16 | 2019-05-14 | Asterias Biotherapeutics, Inc. | Pluripotent stem cell-derived oligodendrocyte progenitor cells for the treatment of spinal cord injury |
| AU2017211858B2 (en) | 2016-01-27 | 2023-05-18 | Memorial Sloan-Kettering Cancer Center | Differentiation of cortical neurons from human pluripotent stem cells |
| CN116121189A (zh) | 2016-03-30 | 2023-05-16 | 阿斯特利亚斯生物治疗股份公司 | 少突胶质细胞祖细胞组合物 |
| EP3518945A4 (en) | 2016-09-14 | 2020-04-22 | Asterias Biotherapeutics, Inc. | OLIGODENDROCYTE PROCUREMENT CELLS FROM PLURIPOTENT STEM CELLS FOR THE TREATMENT OF BACK MARKET LESIONS |
| IL281628B2 (en) | 2018-09-19 | 2025-06-01 | Lineage Cell Therapeutics Inc | Methods for differentiating pluripotent stem cells in dynamic suspension culture |
| WO2020154533A1 (en) | 2019-01-23 | 2020-07-30 | Asterias Biotherapeutics, Inc. | Dorsally-derived oligodendrocyte progenitor cells from human pluripotent stem cells |
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2019
- 2019-09-19 IL IL281628A patent/IL281628B2/en unknown
- 2019-09-19 EP EP19862819.0A patent/EP3853345A4/en active Pending
- 2019-09-19 CN CN202510192544.0A patent/CN120060140A/zh active Pending
- 2019-09-19 US US16/576,627 patent/US20200087622A1/en not_active Abandoned
- 2019-09-19 AU AU2019342741A patent/AU2019342741B2/en active Active
- 2019-09-19 JP JP2021516628A patent/JP7569089B2/ja active Active
- 2019-09-19 CA CA3113640A patent/CA3113640A1/en active Pending
- 2019-09-19 WO PCT/US2019/052015 patent/WO2020061371A2/en not_active Ceased
- 2019-09-19 US US17/277,548 patent/US12365872B2/en active Active
- 2019-09-19 KR KR1020217011488A patent/KR20210068050A/ko active Pending
- 2019-09-19 CN CN201980070640.0A patent/CN113646422B/zh active Active
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2024
- 2024-09-27 JP JP2024169082A patent/JP2024178446A/ja active Pending
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2025
- 2025-06-11 US US19/235,019 patent/US20260002126A1/en active Pending
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