JPWO2019235455A1 - 精子の受精機能増強剤 - Google Patents
精子の受精機能増強剤 Download PDFInfo
- Publication number
- JPWO2019235455A1 JPWO2019235455A1 JP2020523109A JP2020523109A JPWO2019235455A1 JP WO2019235455 A1 JPWO2019235455 A1 JP WO2019235455A1 JP 2020523109 A JP2020523109 A JP 2020523109A JP 2020523109 A JP2020523109 A JP 2020523109A JP WO2019235455 A1 JPWO2019235455 A1 JP WO2019235455A1
- Authority
- JP
- Japan
- Prior art keywords
- group
- carbon atoms
- substituted
- compound
- mmol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003623 enhancer Substances 0.000 title claims abstract description 18
- 230000004720 fertilization Effects 0.000 title claims description 66
- 150000001875 compounds Chemical class 0.000 claims abstract description 286
- 238000000034 method Methods 0.000 claims abstract description 67
- 230000002708 enhancing effect Effects 0.000 claims abstract description 26
- 150000003839 salts Chemical class 0.000 claims abstract description 23
- 238000004519 manufacturing process Methods 0.000 claims abstract description 15
- -1 benzoylmethyl group Chemical group 0.000 claims description 238
- 125000004432 carbon atom Chemical group C* 0.000 claims description 110
- 125000000217 alkyl group Chemical group 0.000 claims description 98
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims description 56
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 47
- 235000013601 eggs Nutrition 0.000 claims description 44
- 239000007864 aqueous solution Substances 0.000 claims description 39
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 32
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims description 32
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims description 32
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 32
- 229910052731 fluorine Inorganic materials 0.000 claims description 26
- 125000000304 alkynyl group Chemical group 0.000 claims description 25
- 125000003342 alkenyl group Chemical group 0.000 claims description 23
- 239000001963 growth medium Substances 0.000 claims description 23
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 23
- 125000003545 alkoxy group Chemical group 0.000 claims description 22
- 239000011737 fluorine Substances 0.000 claims description 20
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims description 19
- 229910052801 chlorine Inorganic materials 0.000 claims description 19
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 14
- 239000000460 chlorine Substances 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 14
- 229910052739 hydrogen Inorganic materials 0.000 claims description 14
- 125000005843 halogen group Chemical group 0.000 claims description 13
- 239000001257 hydrogen Substances 0.000 claims description 12
- 125000001820 oxy group Chemical group [*:1]O[*:2] 0.000 claims description 12
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical group C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims description 9
- 239000005977 Ethylene Chemical group 0.000 claims description 9
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 9
- 125000001511 cyclopentyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C1([H])[H] 0.000 claims description 8
- 125000001033 ether group Chemical group 0.000 claims description 8
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 claims description 8
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims description 7
- 239000002253 acid Substances 0.000 claims description 7
- 125000002947 alkylene group Chemical group 0.000 claims description 7
- 125000001624 naphthyl group Chemical group 0.000 claims description 7
- 125000001041 indolyl group Chemical group 0.000 claims description 6
- 230000003416 augmentation Effects 0.000 claims description 5
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 claims 6
- 150000003384 small molecules Chemical class 0.000 abstract description 5
- 239000004480 active ingredient Substances 0.000 abstract description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 342
- 239000000203 mixture Substances 0.000 description 209
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 195
- 239000000243 solution Substances 0.000 description 174
- 238000006243 chemical reaction Methods 0.000 description 150
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 138
- 238000005481 NMR spectroscopy Methods 0.000 description 133
- 239000002904 solvent Substances 0.000 description 121
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 92
- 239000012044 organic layer Substances 0.000 description 91
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 90
- UHOVQNZJYSORNB-UHFFFAOYSA-N monobenzene Natural products C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 90
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 87
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical class O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 87
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 84
- 238000010898 silica gel chromatography Methods 0.000 description 82
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 80
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 69
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 68
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 57
- 238000003786 synthesis reaction Methods 0.000 description 53
- 230000015572 biosynthetic process Effects 0.000 description 50
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 46
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 45
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 36
- 230000002378 acidificating effect Effects 0.000 description 34
- 238000004992 fast atom bombardment mass spectroscopy Methods 0.000 description 32
- ZCSHNCUQKCANBX-UHFFFAOYSA-N lithium diisopropylamide Chemical compound [Li+].CC(C)[N-]C(C)C ZCSHNCUQKCANBX-UHFFFAOYSA-N 0.000 description 30
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical group [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 27
- 229910052740 iodine Inorganic materials 0.000 description 27
- 230000008018 melting Effects 0.000 description 26
- 238000002844 melting Methods 0.000 description 26
- RIOQSEWOXXDEQQ-UHFFFAOYSA-N triphenylphosphine Chemical compound C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1 RIOQSEWOXXDEQQ-UHFFFAOYSA-N 0.000 description 26
- 239000011630 iodine Substances 0.000 description 25
- GNOIPBMMFNIUFM-UHFFFAOYSA-N hexamethylphosphoric triamide Chemical compound CN(C)P(=O)(N(C)C)N(C)C GNOIPBMMFNIUFM-UHFFFAOYSA-N 0.000 description 24
- 238000002451 electron ionisation mass spectrometry Methods 0.000 description 22
- CSCPPACGZOOCGX-WFGJKAKNSA-N acetone d6 Chemical compound [2H]C([2H])([2H])C(=O)C([2H])([2H])[2H] CSCPPACGZOOCGX-WFGJKAKNSA-N 0.000 description 20
- 239000011541 reaction mixture Substances 0.000 description 20
- GYTCEHNGUPAFMD-ONEGZZNKSA-N (e)-4-(2,4-difluorophenyl)-4-oxobut-2-enoic acid Chemical compound OC(=O)\C=C\C(=O)C1=CC=C(F)C=C1F GYTCEHNGUPAFMD-ONEGZZNKSA-N 0.000 description 19
- LAAHPFCQVWTSIK-UHFFFAOYSA-N 1-propylindole Chemical compound C1=CC=C2N(CCC)C=CC2=C1 LAAHPFCQVWTSIK-UHFFFAOYSA-N 0.000 description 19
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 18
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 18
- 230000009027 insemination Effects 0.000 description 17
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 16
- 238000000338 in vitro Methods 0.000 description 16
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical group C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 16
- ATOYWPVEKTUJHE-UHFFFAOYSA-N methyl 3-(2-methoxy-2-oxoethyl)indole-1-carboxylate Chemical compound C1=CC=C2C(CC(=O)OC)=CN(C(=O)OC)C2=C1 ATOYWPVEKTUJHE-UHFFFAOYSA-N 0.000 description 15
- 0 C[C@@](*)C[C@](C)(C1)N[C@@]2([C@](C)C2)C1=C(C=*)C(*)=C[*+] Chemical compound C[C@@](*)C[C@](C)(C1)N[C@@]2([C@](C)C2)C1=C(C=*)C(*)=C[*+] 0.000 description 14
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 14
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 14
- 239000012299 nitrogen atmosphere Substances 0.000 description 14
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 13
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 13
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 13
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 13
- 239000000047 product Substances 0.000 description 13
- 239000007858 starting material Substances 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 12
- 239000000706 filtrate Substances 0.000 description 12
- 239000007788 liquid Substances 0.000 description 12
- 238000005160 1H NMR spectroscopy Methods 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 11
- 239000013078 crystal Substances 0.000 description 11
- 230000000694 effects Effects 0.000 description 11
- 238000010992 reflux Methods 0.000 description 11
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 11
- 235000019345 sodium thiosulphate Nutrition 0.000 description 11
- 230000002194 synthesizing effect Effects 0.000 description 11
- 125000004215 2,4-difluorophenyl group Chemical group [H]C1=C([H])C(*)=C(F)C([H])=C1F 0.000 description 10
- 150000001555 benzenes Chemical group 0.000 description 10
- 239000012267 brine Substances 0.000 description 10
- 239000012153 distilled water Substances 0.000 description 10
- YNESATAKKCNGOF-UHFFFAOYSA-N lithium bis(trimethylsilyl)amide Chemical compound [Li+].C[Si](C)(C)[N-][Si](C)(C)C YNESATAKKCNGOF-UHFFFAOYSA-N 0.000 description 10
- 125000006239 protecting group Chemical group 0.000 description 10
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 10
- 241000124008 Mammalia Species 0.000 description 9
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 9
- 150000002475 indoles Chemical class 0.000 description 9
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 8
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 8
- 239000011521 glass Substances 0.000 description 8
- 239000003617 indole-3-acetic acid Substances 0.000 description 8
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 150000004702 methyl esters Chemical class 0.000 description 8
- 229920006395 saturated elastomer Polymers 0.000 description 8
- 125000002914 sec-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 8
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 8
- 235000017557 sodium bicarbonate Nutrition 0.000 description 8
- 125000001424 substituent group Chemical group 0.000 description 8
- KTHADMDGDNYQRX-UHFFFAOYSA-N 3-indoleacetic acid methyl ester Natural products C1=CC=C2C(CC(=O)OC)=CNC2=C1 KTHADMDGDNYQRX-UHFFFAOYSA-N 0.000 description 7
- 239000004743 Polypropylene Substances 0.000 description 7
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 7
- 230000009471 action Effects 0.000 description 7
- 208000000509 infertility Diseases 0.000 description 7
- 230000036512 infertility Effects 0.000 description 7
- 231100000535 infertility Toxicity 0.000 description 7
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 description 7
- 239000002480 mineral oil Substances 0.000 description 7
- 235000010446 mineral oil Nutrition 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 229920001155 polypropylene Polymers 0.000 description 7
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 7
- 239000012312 sodium hydride Substances 0.000 description 7
- 229910000104 sodium hydride Inorganic materials 0.000 description 7
- 125000006176 2-ethylbutyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(C([H])([H])*)C([H])([H])C([H])([H])[H] 0.000 description 6
- 125000005916 2-methylpentyl group Chemical group 0.000 description 6
- 125000005917 3-methylpentyl group Chemical group 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 6
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 6
- 125000001309 chloro group Chemical group Cl* 0.000 description 6
- 125000001153 fluoro group Chemical group F* 0.000 description 6
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 6
- ICPIRXLDEXTULL-UHFFFAOYSA-N methyl 2-(5-hydroxy-1h-indol-3-yl)acetate Chemical compound C1=C(O)C=C2C(CC(=O)OC)=CNC2=C1 ICPIRXLDEXTULL-UHFFFAOYSA-N 0.000 description 6
- 229910000027 potassium carbonate Inorganic materials 0.000 description 6
- 239000011734 sodium Substances 0.000 description 6
- 125000006218 1-ethylbutyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C([H])([H])[H] 0.000 description 5
- QJNNHJVSQUUHHE-UHFFFAOYSA-N 2-Methylindole-3-acetic acid Chemical compound C1=CC=C2C(CC(O)=O)=C(C)NC2=C1 QJNNHJVSQUUHHE-UHFFFAOYSA-N 0.000 description 5
- XILOFFNTCYZZIY-UHFFFAOYSA-N CC(C1=CC=CC2=C1C=C(C=C2)O)OC(=O)C Chemical compound CC(C1=CC=CC2=C1C=C(C=C2)O)OC(=O)C XILOFFNTCYZZIY-UHFFFAOYSA-N 0.000 description 5
- BMZRVOVNUMQTIN-UHFFFAOYSA-N Carbonyl Cyanide para-Trifluoromethoxyphenylhydrazone Chemical compound FC(F)(F)OC1=CC=C(NN=C(C#N)C#N)C=C1 BMZRVOVNUMQTIN-UHFFFAOYSA-N 0.000 description 5
- 239000002363 auxin Substances 0.000 description 5
- FJDQFPXHSGXQBY-UHFFFAOYSA-L caesium carbonate Chemical compound [Cs+].[Cs+].[O-]C([O-])=O FJDQFPXHSGXQBY-UHFFFAOYSA-L 0.000 description 5
- 229910000024 caesium carbonate Inorganic materials 0.000 description 5
- 150000001732 carboxylic acid derivatives Chemical group 0.000 description 5
- 239000003054 catalyst Substances 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 125000004491 isohexyl group Chemical group C(CCC(C)C)* 0.000 description 5
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 5
- 230000004899 motility Effects 0.000 description 5
- 125000001280 n-hexyl group Chemical group C(CCCCC)* 0.000 description 5
- 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 5
- 238000001953 recrystallisation Methods 0.000 description 5
- 230000001850 reproductive effect Effects 0.000 description 5
- YQYGGOPUTPQHAY-KIQLFZLRSA-N (4S)-4-[[(2S)-2-[[(2S)-2-[2-[6-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S,3S)-1-[[(2S)-5-amino-1-[[(4S,7R)-7-[[(2S)-1-[(2S)-6-amino-2-[[(2R)-2-[[(2S)-5-amino-2-[[(2S,3R)-2-[[(2S)-6-amino-2-[[(2S)-4-carboxy-2-hydrazinylbutanoyl]amino]hexanoyl]amino]-3-methylpentanoyl]amino]-5-oxopentanoyl]amino]propanoyl]amino]hexanoyl]pyrrolidine-2-carbonyl]amino]-2-methyl-5,6-dioxooctan-4-yl]amino]-1,5-dioxopentan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-5-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3S)-2-[[(2S)-4-amino-2-[[(2S)-2-amino-3-hydroxypropanoyl]amino]-4-oxobutanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-4-carboxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-phenylpropanoyl]amino]-6-oxohexyl]hydrazinyl]-3-phenylpropanoyl]amino]-3-hydroxypropanoyl]amino]-5-[[(2S)-1-[[(2S,3S)-1-[[(2S)-4-amino-1-[[(2S)-1-hydroxy-3-oxopropan-2-yl]amino]-1,4-dioxobutan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-3-hydroxy-1-oxopropan-2-yl]amino]-5-oxopentanoic acid Chemical compound CC[C@@H](C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@H](C)C(=O)C(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](Cc1ccccc1)NC(=O)C(CCCCNN[C@@H](Cc1ccccc1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C=O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CO)[C@H](C)O)C(C)C)[C@H](C)O YQYGGOPUTPQHAY-KIQLFZLRSA-N 0.000 description 4
- 125000005918 1,2-dimethylbutyl group Chemical group 0.000 description 4
- UEMGWPRHOOEKTA-UHFFFAOYSA-N 1,3-difluorobenzene Chemical compound FC1=CC=CC(F)=C1 UEMGWPRHOOEKTA-UHFFFAOYSA-N 0.000 description 4
- QOPBEBWGSGFROG-UHFFFAOYSA-N 2-(1h-indol-2-yl)acetic acid Chemical compound C1=CC=C2NC(CC(=O)O)=CC2=C1 QOPBEBWGSGFROG-UHFFFAOYSA-N 0.000 description 4
- 125000004493 2-methylbut-1-yl group Chemical group CC(C*)CC 0.000 description 4
- 125000003542 3-methylbutan-2-yl group Chemical group [H]C([H])([H])C([H])(*)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 4
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical group [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 4
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 4
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 4
- 238000005727 Friedel-Crafts reaction Methods 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- 238000006957 Michael reaction Methods 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 4
- KXKVLQRXCPHEJC-UHFFFAOYSA-N acetic acid trimethyl ester Natural products COC(C)=O KXKVLQRXCPHEJC-UHFFFAOYSA-N 0.000 description 4
- 150000001414 amino alcohols Chemical class 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- KMGBZBJJOKUPIA-UHFFFAOYSA-N butyl iodide Chemical compound CCCCI KMGBZBJJOKUPIA-UHFFFAOYSA-N 0.000 description 4
- 239000007810 chemical reaction solvent Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 4
- 125000001971 neopentyl group Chemical group [H]C([*])([H])C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 125000003538 pentan-3-yl group Chemical group [H]C([H])([H])C([H])([H])C([H])(*)C([H])([H])C([H])([H])[H] 0.000 description 4
- 239000002504 physiological saline solution Substances 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 125000003548 sec-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 description 4
- XKXIQBVKMABYQJ-UHFFFAOYSA-M tert-butyl carbonate Chemical compound CC(C)(C)OC([O-])=O XKXIQBVKMABYQJ-UHFFFAOYSA-M 0.000 description 4
- KJMOGHNVYHTFRG-UHFFFAOYSA-N tert-butyl n-(4-iodobutyl)carbamate Chemical compound CC(C)(C)OC(=O)NCCCCI KJMOGHNVYHTFRG-UHFFFAOYSA-N 0.000 description 4
- LOAMYYKULCCZAM-UHFFFAOYSA-N tert-butyl n-(6-iodohexyl)carbamate Chemical compound CC(C)(C)OC(=O)NCCCCCCI LOAMYYKULCCZAM-UHFFFAOYSA-N 0.000 description 4
- 125000001973 tert-pentyl group Chemical group [H]C([H])([H])C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 4
- DPKBAXPHAYBPRL-UHFFFAOYSA-M tetrabutylazanium;iodide Chemical compound [I-].CCCC[N+](CCCC)(CCCC)CCCC DPKBAXPHAYBPRL-UHFFFAOYSA-M 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- WFDMCLINTAGONZ-AATRIKPKSA-N (e)-4-(2,4-dimethylphenyl)-4-oxobut-2-enoic acid Chemical compound CC1=CC=C(C(=O)\C=C\C(O)=O)C(C)=C1 WFDMCLINTAGONZ-AATRIKPKSA-N 0.000 description 3
- CMSWGWOQRTZZAS-AATRIKPKSA-N (e)-4-(4-fluorophenyl)-4-oxobut-2-enoic acid Chemical compound OC(=O)\C=C\C(=O)C1=CC=C(F)C=C1 CMSWGWOQRTZZAS-AATRIKPKSA-N 0.000 description 3
- ZQCPBKBTQGUGKL-UHFFFAOYSA-N 1,1,1,2,2-pentafluoro-5-iodopentane Chemical compound FC(F)(F)C(F)(F)CCCI ZQCPBKBTQGUGKL-UHFFFAOYSA-N 0.000 description 3
- UQVVBQGUWVSNAY-UHFFFAOYSA-N 1-iodo-2-methylpentane Chemical compound CCCC(C)CI UQVVBQGUWVSNAY-UHFFFAOYSA-N 0.000 description 3
- VYPSHMOLHNQXBM-UHFFFAOYSA-N 1-iodo-3-methylpentane Chemical compound CCC(C)CCI VYPSHMOLHNQXBM-UHFFFAOYSA-N 0.000 description 3
- 125000004343 1-phenylethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])(*)C([H])([H])[H] 0.000 description 3
- NAIPEFIYIQFVFC-UHFFFAOYSA-N 2-(1-methylindol-3-yl)acetic acid Chemical compound C1=CC=C2N(C)C=C(CC(O)=O)C2=C1 NAIPEFIYIQFVFC-UHFFFAOYSA-N 0.000 description 3
- FIBICICXPCMTRV-UHFFFAOYSA-N 2-(7-hydroxynaphthalen-1-yl)acetic acid Chemical compound C1=C(O)C=C2C(CC(=O)O)=CC=CC2=C1 FIBICICXPCMTRV-UHFFFAOYSA-N 0.000 description 3
- NRZNTGUFHSJBTD-HKOYGPOVSA-N 2-[2-(2-methoxyethoxy)ethoxy]ethyl (e)-2-cyano-3-(6-piperidin-1-ylnaphthalen-2-yl)prop-2-enoate Chemical compound C1=CC2=CC(/C=C(C(=O)OCCOCCOCCOC)\C#N)=CC=C2C=C1N1CCCCC1 NRZNTGUFHSJBTD-HKOYGPOVSA-N 0.000 description 3
- QMSCSRFFXLJBFU-UHFFFAOYSA-N 2-iodoethylcyclopentane Chemical compound ICCC1CCCC1 QMSCSRFFXLJBFU-UHFFFAOYSA-N 0.000 description 3
- 125000000094 2-phenylethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])* 0.000 description 3
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 3
- VNDWQCSOSCCWIP-UHFFFAOYSA-N 2-tert-butyl-9-fluoro-1,6-dihydrobenzo[h]imidazo[4,5-f]isoquinolin-7-one Chemical compound C1=2C=CNC(=O)C=2C2=CC(F)=CC=C2C2=C1NC(C(C)(C)C)=N2 VNDWQCSOSCCWIP-UHFFFAOYSA-N 0.000 description 3
- FQQKJFCXOKMBTA-UHFFFAOYSA-N 3-(iodomethyl)pentane Chemical compound CCC(CC)CI FQQKJFCXOKMBTA-UHFFFAOYSA-N 0.000 description 3
- 125000004975 3-butenyl group Chemical group C(CC=C)* 0.000 description 3
- SVLZRCRXNHITBY-UHFFFAOYSA-N 4-chloro-1h-indole Chemical compound ClC1=CC=CC2=C1C=CN2 SVLZRCRXNHITBY-UHFFFAOYSA-N 0.000 description 3
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 3
- 229930192334 Auxin Natural products 0.000 description 3
- 241000282693 Cercopithecidae Species 0.000 description 3
- 239000002841 Lewis acid Substances 0.000 description 3
- 208000007466 Male Infertility Diseases 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 3
- WETWJCDKMRHUPV-UHFFFAOYSA-N acetyl chloride Chemical compound CC(Cl)=O WETWJCDKMRHUPV-UHFFFAOYSA-N 0.000 description 3
- 239000012346 acetyl chloride Substances 0.000 description 3
- 235000019270 ammonium chloride Nutrition 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 210000000918 epididymis Anatomy 0.000 description 3
- 201000010063 epididymitis Diseases 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 3
- 150000002431 hydrogen Chemical class 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- INQOMBQAUSQDDS-UHFFFAOYSA-N iodomethane Chemical compound IC INQOMBQAUSQDDS-UHFFFAOYSA-N 0.000 description 3
- DUMSKQUKLVSSII-UHFFFAOYSA-N iodomethylcyclopentane Chemical compound ICC1CCCC1 DUMSKQUKLVSSII-UHFFFAOYSA-N 0.000 description 3
- 125000003253 isopropoxy group Chemical group [H]C([H])([H])C([H])(O*)C([H])([H])[H] 0.000 description 3
- 150000007517 lewis acids Chemical class 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- SQOPSAUMHVFOTL-UHFFFAOYSA-N methyl 2-(1-hexylindol-3-yl)acetate Chemical compound C1=CC=C2N(CCCCCC)C=C(CC(=O)OC)C2=C1 SQOPSAUMHVFOTL-UHFFFAOYSA-N 0.000 description 3
- IRUCAIUHUJYOGN-UHFFFAOYSA-N methyl 2-(5-propoxy-1h-indol-3-yl)acetate Chemical compound CCCOC1=CC=C2NC=C(CC(=O)OC)C2=C1 IRUCAIUHUJYOGN-UHFFFAOYSA-N 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 238000006053 organic reaction Methods 0.000 description 3
- 108010058237 plasma protein fraction Proteins 0.000 description 3
- 229940081857 plasma protein fraction Drugs 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 125000002572 propoxy group Chemical group [*]OC([H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 235000002020 sage Nutrition 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- YBNJZIDYXCGAPX-UHFFFAOYSA-N tert-butyl 4-(2-hydroxyethyl)piperidine-1-carboxylate Chemical compound CC(C)(C)OC(=O)N1CCC(CCO)CC1 YBNJZIDYXCGAPX-UHFFFAOYSA-N 0.000 description 3
- LIYMTLVBAVHPBU-UHFFFAOYSA-N tert-butyl n-(4-hydroxybutyl)carbamate Chemical compound CC(C)(C)OC(=O)NCCCCO LIYMTLVBAVHPBU-UHFFFAOYSA-N 0.000 description 3
- BDLPJHZUTLGFON-UHFFFAOYSA-N tert-butyl n-(6-hydroxyhexyl)carbamate Chemical compound CC(C)(C)OC(=O)NCCCCCCO BDLPJHZUTLGFON-UHFFFAOYSA-N 0.000 description 3
- KSFVNEXYCULLEJ-UHFFFAOYSA-N tert-butyl n-[2-(2-hydroxyethoxy)ethyl]carbamate Chemical compound CC(C)(C)OC(=O)NCCOCCO KSFVNEXYCULLEJ-UHFFFAOYSA-N 0.000 description 3
- RGHXWCUQSIGAJS-UHFFFAOYSA-N tert-butyl n-[2-(2-iodoethoxy)ethyl]carbamate Chemical compound CC(C)(C)OC(=O)NCCOCCI RGHXWCUQSIGAJS-UHFFFAOYSA-N 0.000 description 3
- 210000004291 uterus Anatomy 0.000 description 3
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 125000005926 1,2-dimethylbutyloxy group Chemical group 0.000 description 2
- 125000005923 1,2-dimethylpropyloxy group Chemical group 0.000 description 2
- PCARGECGQUVALM-UHFFFAOYSA-N 1-(2-iodoethyl)-3-phenylbenzene Chemical group ICCC1=CC=CC(C=2C=CC=CC=2)=C1 PCARGECGQUVALM-UHFFFAOYSA-N 0.000 description 2
- 125000004973 1-butenyl group Chemical group C(=CCC)* 0.000 description 2
- 125000004972 1-butynyl group Chemical group [H]C([H])([H])C([H])([H])C#C* 0.000 description 2
- ORLCYMQZIPSODD-UHFFFAOYSA-N 1-chloro-2-[chloro(2,2,2-trichloroethoxy)phosphoryl]oxybenzene Chemical compound ClC1=CC=CC=C1OP(Cl)(=O)OCC(Cl)(Cl)Cl ORLCYMQZIPSODD-UHFFFAOYSA-N 0.000 description 2
- 125000006039 1-hexenyl group Chemical group 0.000 description 2
- 125000006017 1-propenyl group Chemical group 0.000 description 2
- 125000000530 1-propynyl group Chemical group [H]C([H])([H])C#C* 0.000 description 2
- OEANUFXHQRCPQY-UHFFFAOYSA-N 2-(1-butylindol-3-yl)acetic acid Chemical compound C1=CC=C2N(CCCC)C=C(CC(O)=O)C2=C1 OEANUFXHQRCPQY-UHFFFAOYSA-N 0.000 description 2
- FHSUAUTWQFUCHG-UHFFFAOYSA-N 2-(1-heptylindol-3-yl)acetic acid Chemical compound C1=CC=C2N(CCCCCCC)C=C(CC(O)=O)C2=C1 FHSUAUTWQFUCHG-UHFFFAOYSA-N 0.000 description 2
- QSLMJQGZLVYSCT-UHFFFAOYSA-N 2-(1-hexylindol-3-yl)acetic acid Chemical compound C1=CC=C2N(CCCCCC)C=C(CC(O)=O)C2=C1 QSLMJQGZLVYSCT-UHFFFAOYSA-N 0.000 description 2
- NLADHTWPXLVAJK-UHFFFAOYSA-N 2-(1-propylindol-3-yl)acetic acid Chemical compound C1=CC=C2N(CCC)C=C(CC(O)=O)C2=C1 NLADHTWPXLVAJK-UHFFFAOYSA-N 0.000 description 2
- QCKDYXGUHJCUAF-UHFFFAOYSA-N 2-(1h-indol-3-yl)-4-methylheptanoic acid Chemical compound C1=CC=C2C(C(C(O)=O)CC(C)CCC)=CNC2=C1 QCKDYXGUHJCUAF-UHFFFAOYSA-N 0.000 description 2
- SJVMWLJNHPHNPT-UHFFFAOYSA-N 2-(1h-indol-3-yl)-4-oxo-4-phenylbutanoic acid Chemical compound C=1NC2=CC=CC=C2C=1C(C(=O)O)CC(=O)C1=CC=CC=C1 SJVMWLJNHPHNPT-UHFFFAOYSA-N 0.000 description 2
- RDOJTDHHUDULPS-UHFFFAOYSA-N 2-(1h-indol-3-yl)-4-phenylbutanoic acid Chemical compound C=1NC2=CC=CC=C2C=1C(C(=O)O)CCC1=CC=CC=C1 RDOJTDHHUDULPS-UHFFFAOYSA-N 0.000 description 2
- UUDSZRCHBNKYNV-UHFFFAOYSA-N 2-(1h-indol-3-yl)-5-methylheptanoic acid Chemical compound C1=CC=C2C(C(C(O)=O)CCC(C)CC)=CNC2=C1 UUDSZRCHBNKYNV-UHFFFAOYSA-N 0.000 description 2
- NOPMBRCHCNZTDW-UHFFFAOYSA-N 2-(3-phenylphenyl)ethanol Chemical group OCCC1=CC=CC(C=2C=CC=CC=2)=C1 NOPMBRCHCNZTDW-UHFFFAOYSA-N 0.000 description 2
- BMUJWOWDEAQXAI-UHFFFAOYSA-N 2-(4-chloro-1H-indol-3-yl)-4-(2,4-difluorophenyl)-4-oxobutanoic acid Chemical compound ClC1=C2C(=CNC2=CC=C1)C(C(=O)O)CC(=O)C1=C(C=C(C=C1)F)F BMUJWOWDEAQXAI-UHFFFAOYSA-N 0.000 description 2
- CDOZXYNYHFCGRZ-UHFFFAOYSA-N 2-(5-butoxy-1h-indol-3-yl)acetic acid Chemical compound CCCCOC1=CC=C2NC=C(CC(O)=O)C2=C1 CDOZXYNYHFCGRZ-UHFFFAOYSA-N 0.000 description 2
- JADLKAXYGRNFKN-UHFFFAOYSA-N 2-(5-ethoxy-1h-indol-3-yl)acetic acid Chemical compound CCOC1=CC=C2NC=C(CC(O)=O)C2=C1 JADLKAXYGRNFKN-UHFFFAOYSA-N 0.000 description 2
- RDISIAAAWVDPMV-UHFFFAOYSA-N 2-(5-propoxy-1h-indol-3-yl)acetic acid Chemical compound CCCOC1=CC=C2NC=C(CC(O)=O)C2=C1 RDISIAAAWVDPMV-UHFFFAOYSA-N 0.000 description 2
- VVNTYBGNXYDJHS-UHFFFAOYSA-N 2-(7-butoxynaphthalen-1-yl)acetic acid Chemical compound C1=CC=C(CC(O)=O)C2=CC(OCCCC)=CC=C21 VVNTYBGNXYDJHS-UHFFFAOYSA-N 0.000 description 2
- DECXYLYCPGDOHZ-UHFFFAOYSA-N 2-(7-pentoxynaphthalen-1-yl)acetic acid Chemical compound C1=CC=C(CC(O)=O)C2=CC(OCCCCC)=CC=C21 DECXYLYCPGDOHZ-UHFFFAOYSA-N 0.000 description 2
- MZSYAASXDPWPAL-UHFFFAOYSA-N 2-[5-[(3,5-dimethoxyphenyl)methoxy]-1h-indol-3-yl]acetic acid Chemical compound COC1=CC(OC)=CC(COC=2C=C3C(CC(O)=O)=CNC3=CC=2)=C1 MZSYAASXDPWPAL-UHFFFAOYSA-N 0.000 description 2
- 125000004974 2-butenyl group Chemical group C(C=CC)* 0.000 description 2
- 125000003858 2-ethylbutoxy group Chemical group [H]C([H])([H])C([H])([H])C([H])(C([H])([H])O*)C([H])([H])C([H])([H])[H] 0.000 description 2
- PFNHSEQQEPMLNI-UHFFFAOYSA-N 2-methyl-1-pentanol Chemical compound CCCC(C)CO PFNHSEQQEPMLNI-UHFFFAOYSA-N 0.000 description 2
- 125000005924 2-methylpentyloxy group Chemical group 0.000 description 2
- 125000001331 3-methylbutoxy group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])O* 0.000 description 2
- IWTBVKIGCDZRPL-UHFFFAOYSA-N 3-methylpentanol Chemical compound CCC(C)CCO IWTBVKIGCDZRPL-UHFFFAOYSA-N 0.000 description 2
- 125000005925 3-methylpentyloxy group Chemical group 0.000 description 2
- QROUUECTKRZFHF-UHFFFAOYSA-N 4,4,5,5,5-pentafluoropentan-1-ol Chemical compound OCCCC(F)(F)C(F)(F)F QROUUECTKRZFHF-UHFFFAOYSA-N 0.000 description 2
- BLFRQYKZFKYQLO-UHFFFAOYSA-N 4-aminobutan-1-ol Chemical compound NCCCCO BLFRQYKZFKYQLO-UHFFFAOYSA-N 0.000 description 2
- PYKJQTWMTMHYOI-UHFFFAOYSA-N 4-ethyl-2-(1h-indol-3-yl)hexanoic acid Chemical compound C1=CC=C2C(C(C(O)=O)CC(CC)CC)=CNC2=C1 PYKJQTWMTMHYOI-UHFFFAOYSA-N 0.000 description 2
- 125000000439 4-methylpentoxy group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 2
- LLKFNPUXQZHIAE-UHFFFAOYSA-N 5-(3-aminopropyl)-8-bromo-3-methyl-2h-pyrazolo[4,3-c]quinolin-4-one Chemical compound O=C1N(CCCN)C2=CC=C(Br)C=C2C2=C1C(C)=NN2 LLKFNPUXQZHIAE-UHFFFAOYSA-N 0.000 description 2
- COCNDHOPIHDTHK-UHFFFAOYSA-N 5-methoxyindole-3-acetic acid Chemical compound COC1=CC=C2NC=C(CC(O)=O)C2=C1 COCNDHOPIHDTHK-UHFFFAOYSA-N 0.000 description 2
- YPKBCLZFIYBSHK-UHFFFAOYSA-N 5-methylindole Chemical compound CC1=CC=C2NC=CC2=C1 YPKBCLZFIYBSHK-UHFFFAOYSA-N 0.000 description 2
- YYFFEPUCAKVRJX-UHFFFAOYSA-N 6-fluoro-1h-indole Chemical compound FC1=CC=C2C=CNC2=C1 YYFFEPUCAKVRJX-UHFFFAOYSA-N 0.000 description 2
- ONYNOPPOVKYGRS-UHFFFAOYSA-N 6-methylindole Natural products CC1=CC=C2C=CNC2=C1 ONYNOPPOVKYGRS-UHFFFAOYSA-N 0.000 description 2
- YNERCODOMUOPNM-UHFFFAOYSA-N 8-[(2-methylpropan-2-yl)oxycarbonylamino]-2-naphthalen-1-yloctanoic acid Chemical compound C1=CC=C2C(C(C(O)=O)CCCCCCNC(=O)OC(C)(C)C)=CC=CC2=C1 YNERCODOMUOPNM-UHFFFAOYSA-N 0.000 description 2
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 2
- 101150093547 AUX1 gene Proteins 0.000 description 2
- FOXAGCVOBJCWHU-UHFFFAOYSA-N C(=O)=C(C(=O)O)C1=CNC2=CC=CC=C12 Chemical compound C(=O)=C(C(=O)O)C1=CNC2=CC=CC=C12 FOXAGCVOBJCWHU-UHFFFAOYSA-N 0.000 description 2
- AHYOYCPRKZZQDA-UHFFFAOYSA-N C(C)(=O)N1C(CCC1)C(=O)NCCCCC(C(=O)O)C1=CNC2=CC=CC=C12 Chemical compound C(C)(=O)N1C(CCC1)C(=O)NCCCCC(C(=O)O)C1=CNC2=CC=CC=C12 AHYOYCPRKZZQDA-UHFFFAOYSA-N 0.000 description 2
- LAXAPRUTPLLDQI-UHFFFAOYSA-N CCCCCOC1=CC2=C(C=CC=C2C(C)OC(=O)C)C=C1 Chemical compound CCCCCOC1=CC2=C(C=CC=C2C(C)OC(=O)C)C=C1 LAXAPRUTPLLDQI-UHFFFAOYSA-N 0.000 description 2
- JNTSVFDXOZTBEK-UHFFFAOYSA-N CCCCOC1=CC=C(C=CC=C2C(C)OC(C)=O)C2=C1 Chemical compound CCCCOC1=CC=C(C=CC=C2C(C)OC(C)=O)C2=C1 JNTSVFDXOZTBEK-UHFFFAOYSA-N 0.000 description 2
- YORJFUUHAZHVTQ-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)C)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)C)=O YORJFUUHAZHVTQ-UHFFFAOYSA-N 0.000 description 2
- YHODOKORTPQKAF-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCCNC(=O)OC(C)(C)C)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCCNC(=O)OC(C)(C)C)=O YHODOKORTPQKAF-UHFFFAOYSA-N 0.000 description 2
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000282326 Felis catus Species 0.000 description 2
- 108091006905 Human Serum Albumin Proteins 0.000 description 2
- 102000008100 Human Serum Albumin Human genes 0.000 description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 2
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 description 2
- MZRVEZGGRBJDDB-UHFFFAOYSA-N N-Butyllithium Chemical compound [Li]CCCC MZRVEZGGRBJDDB-UHFFFAOYSA-N 0.000 description 2
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 2
- PCLIMKBDDGJMGD-UHFFFAOYSA-N N-bromosuccinimide Chemical compound BrN1C(=O)CCC1=O PCLIMKBDDGJMGD-UHFFFAOYSA-N 0.000 description 2
- JRNVZBWKYDBUCA-UHFFFAOYSA-N N-chlorosuccinimide Chemical compound ClN1C(=O)CCC1=O JRNVZBWKYDBUCA-UHFFFAOYSA-N 0.000 description 2
- LQZMLBORDGWNPD-UHFFFAOYSA-N N-iodosuccinimide Chemical compound IN1C(=O)CCC1=O LQZMLBORDGWNPD-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 101100367246 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SWA2 gene Proteins 0.000 description 2
- 229940124639 Selective inhibitor Drugs 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 229910000288 alkali metal carbonate Inorganic materials 0.000 description 2
- 150000008041 alkali metal carbonates Chemical class 0.000 description 2
- 150000001347 alkyl bromides Chemical class 0.000 description 2
- 150000001351 alkyl iodides Chemical class 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 229910052786 argon Inorganic materials 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 2
- 229910052794 bromium Inorganic materials 0.000 description 2
- 150000001721 carbon Chemical group 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000012230 colorless oil Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000003750 conditioning effect Effects 0.000 description 2
- NZNMSOFKMUBTKW-UHFFFAOYSA-N cyclohexanecarboxylic acid Chemical compound OC(=O)C1CCCCC1 NZNMSOFKMUBTKW-UHFFFAOYSA-N 0.000 description 2
- 125000004851 cyclopentylmethyl group Chemical group C1(CCCC1)C* 0.000 description 2
- 238000005886 esterification reaction Methods 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 125000002534 ethynyl group Chemical group [H]C#C* 0.000 description 2
- 210000002503 granulosa cell Anatomy 0.000 description 2
- 150000002366 halogen compounds Chemical class 0.000 description 2
- 230000002140 halogenating effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- HVTICUPFWKNHNG-UHFFFAOYSA-N iodoethane Chemical compound CCI HVTICUPFWKNHNG-UHFFFAOYSA-N 0.000 description 2
- 125000002510 isobutoxy group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])O* 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229910052744 lithium Inorganic materials 0.000 description 2
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 2
- 229910052808 lithium carbonate Inorganic materials 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- KHQOQPGDGWABAW-UHFFFAOYSA-N methyl 2-(1-butylindol-3-yl)acetate Chemical compound C1=CC=C2N(CCCC)C=C(CC(=O)OC)C2=C1 KHQOQPGDGWABAW-UHFFFAOYSA-N 0.000 description 2
- GWWLCSWSZRRLLX-UHFFFAOYSA-N methyl 2-(1-propylindol-3-yl)acetate Chemical compound C1=CC=C2N(CCC)C=C(CC(=O)OC)C2=C1 GWWLCSWSZRRLLX-UHFFFAOYSA-N 0.000 description 2
- MTSOPGXBIBOMDV-UHFFFAOYSA-N methyl 2-(5-ethoxy-1h-indol-3-yl)acetate Chemical compound CCOC1=CC=C2NC=C(CC(=O)OC)C2=C1 MTSOPGXBIBOMDV-UHFFFAOYSA-N 0.000 description 2
- RCYOGUDXQWZUCD-UHFFFAOYSA-N methyl 3-(1-methoxy-4-methyl-1-oxoheptan-2-yl)indole-1-carboxylate Chemical compound C1=CC=C2C(C(C(=O)OC)CC(C)CCC)=CN(C(=O)OC)C2=C1 RCYOGUDXQWZUCD-UHFFFAOYSA-N 0.000 description 2
- KQDSETGPLOZNSU-UHFFFAOYSA-N methyl 3-(1-methoxy-5-methyl-1-oxoheptan-2-yl)indole-1-carboxylate Chemical compound C1=CC=C2C(C(C(=O)OC)CCC(C)CC)=CN(C(=O)OC)C2=C1 KQDSETGPLOZNSU-UHFFFAOYSA-N 0.000 description 2
- TZIHFWKZFHZASV-UHFFFAOYSA-N methyl formate Chemical compound COC=O TZIHFWKZFHZASV-UHFFFAOYSA-N 0.000 description 2
- 230000002438 mitochondrial effect Effects 0.000 description 2
- 125000006606 n-butoxy group Chemical group 0.000 description 2
- 125000003935 n-pentoxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 2
- PVWOIHVRPOBWPI-UHFFFAOYSA-N n-propyl iodide Chemical compound CCCI PVWOIHVRPOBWPI-UHFFFAOYSA-N 0.000 description 2
- 238000007344 nucleophilic reaction Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 230000016087 ovulation Effects 0.000 description 2
- NFHFRUOZVGFOOS-UHFFFAOYSA-N palladium;triphenylphosphane Chemical compound [Pd].C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1.C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1.C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1.C1=CC=CC=C1P(C=1C=CC=CC=1)C1=CC=CC=C1 NFHFRUOZVGFOOS-UHFFFAOYSA-N 0.000 description 2
- HXITXNWTGFUOAU-UHFFFAOYSA-N phenylboronic acid Chemical compound OB(O)C1=CC=CC=C1 HXITXNWTGFUOAU-UHFFFAOYSA-N 0.000 description 2
- 125000004482 piperidin-4-yl group Chemical group N1CCC(CC1)* 0.000 description 2
- 239000002798 polar solvent Substances 0.000 description 2
- 229920000137 polyphosphoric acid Polymers 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 125000005920 sec-butoxy group Chemical group 0.000 description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
- 235000011152 sodium sulphate Nutrition 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- UIUJIQZEACWQSV-UHFFFAOYSA-N succinic semialdehyde Chemical compound OC(=O)CCC=O UIUJIQZEACWQSV-UHFFFAOYSA-N 0.000 description 2
- 125000004213 tert-butoxy group Chemical group [H]C([H])([H])C(O*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
- 125000000383 tetramethylene group Chemical group [H]C([H])([*:1])C([H])([H])C([H])([H])C([H])([H])[*:2] 0.000 description 2
- 239000001618 (3R)-3-methylpentan-1-ol Substances 0.000 description 1
- ZZCYWRNODYRNPJ-UHFFFAOYSA-N (7-hydroxynaphthalen-1-yl) acetate Chemical compound CC(=O)Oc1cccc2ccc(O)cc12 ZZCYWRNODYRNPJ-UHFFFAOYSA-N 0.000 description 1
- 125000006273 (C1-C3) alkyl group Chemical group 0.000 description 1
- PLPDHGOODMBBGN-VOTSOKGWSA-N (e)-4-oxo-4-phenylbut-2-enoic acid Chemical compound OC(=O)\C=C\C(=O)C1=CC=CC=C1 PLPDHGOODMBBGN-VOTSOKGWSA-N 0.000 description 1
- BTHIGJGJAPYFSJ-UHFFFAOYSA-N 1-(bromomethyl)-3,5-dimethoxybenzene Chemical compound COC1=CC(CBr)=CC(OC)=C1 BTHIGJGJAPYFSJ-UHFFFAOYSA-N 0.000 description 1
- 125000006219 1-ethylpentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C([H])([H])[H] 0.000 description 1
- LMHCYRULPLGEEZ-UHFFFAOYSA-N 1-iodoheptane Chemical compound CCCCCCCI LMHCYRULPLGEEZ-UHFFFAOYSA-N 0.000 description 1
- ANOOTOPTCJRUPK-UHFFFAOYSA-N 1-iodohexane Chemical compound CCCCCCI ANOOTOPTCJRUPK-UHFFFAOYSA-N 0.000 description 1
- BLXSFCHWMBESKV-UHFFFAOYSA-N 1-iodopentane Chemical compound CCCCCI BLXSFCHWMBESKV-UHFFFAOYSA-N 0.000 description 1
- BLRHMMGNCXNXJL-UHFFFAOYSA-N 1-methylindole Chemical compound C1=CC=C2N(C)C=CC2=C1 BLRHMMGNCXNXJL-UHFFFAOYSA-N 0.000 description 1
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- 125000006023 1-pentenyl group Chemical group 0.000 description 1
- 125000000453 2,2,2-trichloroethyl group Chemical group [H]C([H])(*)C(Cl)(Cl)Cl 0.000 description 1
- QOQGUYAEXRBZQN-UHFFFAOYSA-N 2,3-dichloro-1h-indole Chemical compound C1=CC=C2C(Cl)=C(Cl)NC2=C1 QOQGUYAEXRBZQN-UHFFFAOYSA-N 0.000 description 1
- NCHHYGWGXAVANC-UHFFFAOYSA-N 2-(1-ethylindol-3-yl)acetic acid Chemical compound C1=CC=C2N(CC)C=C(CC(O)=O)C2=C1 NCHHYGWGXAVANC-UHFFFAOYSA-N 0.000 description 1
- SPUOQYBQXFYATF-UHFFFAOYSA-N 2-(1h-indol-3-yl)-2-methyl-8-[(2-methylpropan-2-yl)oxycarbonylamino]octanoic acid Chemical compound C1=CC=C2C(C(C)(C(O)=O)CCCCCCNC(=O)OC(C)(C)C)=CNC2=C1 SPUOQYBQXFYATF-UHFFFAOYSA-N 0.000 description 1
- NASLQMVIHGMBFW-UHFFFAOYSA-N 2-(1h-indol-3-yl)-4-(3-phenylphenyl)butanoic acid Chemical compound C=1NC2=CC=CC=C2C=1C(C(=O)O)CCC(C=1)=CC=CC=1C1=CC=CC=C1 NASLQMVIHGMBFW-UHFFFAOYSA-N 0.000 description 1
- IIILSMOCRIAUGU-UHFFFAOYSA-N 2-(1h-indol-3-yl)-4-[2-[(2-methylpropan-2-yl)oxycarbonylamino]ethoxy]butanoic acid Chemical compound C1=CC=C2C(C(C(O)=O)CCOCCNC(=O)OC(C)(C)C)=CNC2=C1 IIILSMOCRIAUGU-UHFFFAOYSA-N 0.000 description 1
- WRTLGPFGOPOWJY-UHFFFAOYSA-N 2-(1h-indol-3-yl)-6-[(2-methylpropan-2-yl)oxycarbonylamino]hexanoic acid Chemical compound C1=CC=C2C(C(C(O)=O)CCCCNC(=O)OC(C)(C)C)=CNC2=C1 WRTLGPFGOPOWJY-UHFFFAOYSA-N 0.000 description 1
- GIAFURWZWWWBQT-UHFFFAOYSA-N 2-(2-aminoethoxy)ethanol Chemical compound NCCOCCO GIAFURWZWWWBQT-UHFFFAOYSA-N 0.000 description 1
- PTTFLKHCSZSFOL-UHFFFAOYSA-N 2-(3-bromophenyl)ethanol Chemical compound OCCC1=CC=CC(Br)=C1 PTTFLKHCSZSFOL-UHFFFAOYSA-N 0.000 description 1
- ANMMJRBZBKGNSC-UHFFFAOYSA-N 2-(5,6-dichloro-1h-indol-3-yl)propanoic acid Chemical compound ClC1=C(Cl)C=C2C(C(C(O)=O)C)=CNC2=C1 ANMMJRBZBKGNSC-UHFFFAOYSA-N 0.000 description 1
- PEJLFYUQYZGQRX-UHFFFAOYSA-N 2-(5-chloro-1H-indol-3-yl)-4-(2,4-difluorophenyl)-4-oxobutanoic acid Chemical compound ClC=1C=C2C(=CNC2=CC=1)C(C(=O)O)CC(=O)C1=C(C=C(C=C1)F)F PEJLFYUQYZGQRX-UHFFFAOYSA-N 0.000 description 1
- MVZKODDAEICLIQ-UHFFFAOYSA-N 2-(7-chloro-1H-indol-3-yl)-4-(2,4-difluorophenyl)-4-oxobutanoic acid Chemical compound ClC=1C=CC=C2C(=CNC=12)C(C(=O)O)CC(=O)C1=C(C=C(C=C1)F)F MVZKODDAEICLIQ-UHFFFAOYSA-N 0.000 description 1
- PYSRGWAMLYAJNO-UHFFFAOYSA-N 2-(7-hydroxy-1h-indol-3-yl)acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1O PYSRGWAMLYAJNO-UHFFFAOYSA-N 0.000 description 1
- LBLYYCQCTBFVLH-UHFFFAOYSA-N 2-Methylbenzenesulfonic acid Chemical compound CC1=CC=CC=C1S(O)(=O)=O LBLYYCQCTBFVLH-UHFFFAOYSA-N 0.000 description 1
- NGNBDVOYPDDBFK-UHFFFAOYSA-N 2-[2,4-di(pentan-2-yl)phenoxy]acetyl chloride Chemical compound CCCC(C)C1=CC=C(OCC(Cl)=O)C(C(C)CCC)=C1 NGNBDVOYPDDBFK-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- 125000000069 2-butynyl group Chemical group [H]C([H])([H])C#CC([H])([H])* 0.000 description 1
- UBVONCJSPBLTPN-UHFFFAOYSA-N 2-cyclopentyl-2-(2-methyl-1h-indol-3-yl)acetic acid Chemical compound CC=1NC2=CC=CC=C2C=1C(C(O)=O)C1CCCC1 UBVONCJSPBLTPN-UHFFFAOYSA-N 0.000 description 1
- JEXQWCBPEWHFKC-UHFFFAOYSA-N 2-cyclopentylethanol Chemical compound OCCC1CCCC1 JEXQWCBPEWHFKC-UHFFFAOYSA-N 0.000 description 1
- TZYRSLHNPKPEFV-UHFFFAOYSA-N 2-ethyl-1-butanol Chemical compound CCC(CC)CO TZYRSLHNPKPEFV-UHFFFAOYSA-N 0.000 description 1
- 125000006040 2-hexenyl group Chemical group 0.000 description 1
- 125000004200 2-methoxyethyl group Chemical group [H]C([H])([H])OC([H])([H])C([H])([H])* 0.000 description 1
- 125000003229 2-methylhexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 1
- TYEYBOSBBBHJIV-UHFFFAOYSA-N 2-oxobutanoic acid Chemical compound CCC(=O)C(O)=O TYEYBOSBBBHJIV-UHFFFAOYSA-N 0.000 description 1
- 125000006024 2-pentenyl group Chemical group 0.000 description 1
- WMPPDTMATNBGJN-UHFFFAOYSA-N 2-phenylethylbromide Chemical compound BrCCC1=CC=CC=C1 WMPPDTMATNBGJN-UHFFFAOYSA-N 0.000 description 1
- LDSQQXKSEFZAPE-UHFFFAOYSA-N 2-piperidin-4-ylethanol Chemical compound OCCC1CCNCC1 LDSQQXKSEFZAPE-UHFFFAOYSA-N 0.000 description 1
- 125000001494 2-propynyl group Chemical group [H]C#CC([H])([H])* 0.000 description 1
- CPYMXYDBEVHMFN-UHFFFAOYSA-N 3-(1h-indol-3-yl)-1-phenylbutan-1-one Chemical compound C=1NC2=CC=CC=C2C=1C(C)CC(=O)C1=CC=CC=C1 CPYMXYDBEVHMFN-UHFFFAOYSA-N 0.000 description 1
- 125000000474 3-butynyl group Chemical group [H]C#CC([H])([H])C([H])([H])* 0.000 description 1
- REDLKIOYAMBCIV-UHFFFAOYSA-N 3-cyclopentyl-2-(1h-indol-3-yl)propanoic acid Chemical compound C=1NC2=CC=CC=C2C=1C(C(=O)O)CC1CCCC1 REDLKIOYAMBCIV-UHFFFAOYSA-N 0.000 description 1
- 125000004337 3-ethylpentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])(C([H])([H])C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000006041 3-hexenyl group Chemical group 0.000 description 1
- 125000003469 3-methylhexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000006506 3-phenyl benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C1=C([H])C([H])=C([H])C(=C1[H])C([H])([H])* 0.000 description 1
- GAZGOKMXOJFIGO-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-2-(1-methylindol-3-yl)-4-oxobutanoic acid Chemical compound FC1=C(C=CC(=C1)F)C(CC(C(=O)O)C1=CN(C2=CC=CC=C12)C)=O GAZGOKMXOJFIGO-UHFFFAOYSA-N 0.000 description 1
- BOKQALWNGNLTOC-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-2-(1h-indol-3-yl)-4-oxobutanoic acid Chemical compound C=1NC2=CC=CC=C2C=1C(C(=O)O)CC(=O)C1=CC=C(F)C=C1F BOKQALWNGNLTOC-UHFFFAOYSA-N 0.000 description 1
- UOGWMENCHSEKML-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-2-(5-fluoro-1H-indol-3-yl)-4-oxobutanoic acid Chemical compound FC1=C(C=CC(=C1)F)C(CC(C(=O)O)C1=CNC2=CC=C(C=C12)F)=O UOGWMENCHSEKML-UHFFFAOYSA-N 0.000 description 1
- PPPFOINQKKKMKK-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-2-(5-methoxy-1H-indol-3-yl)-4-oxobutanoic acid Chemical compound FC1=C(C=CC(=C1)F)C(CC(C(=O)O)C1=CNC2=CC=C(C=C12)OC)=O PPPFOINQKKKMKK-UHFFFAOYSA-N 0.000 description 1
- IAVHULVSZFKIBQ-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-2-(5-methyl-1H-indol-3-yl)-4-oxobutanoic acid Chemical compound FC1=C(C=CC(=C1)F)C(CC(C(=O)O)C1=CNC2=CC=C(C=C12)C)=O IAVHULVSZFKIBQ-UHFFFAOYSA-N 0.000 description 1
- GSMHZSAVQYHPAG-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-2-(6-fluoro-1H-indol-3-yl)-4-oxobutanoic acid Chemical compound FC1=C(C=CC(=C1)F)C(CC(C(=O)O)C1=CNC2=CC(=CC=C12)F)=O GSMHZSAVQYHPAG-UHFFFAOYSA-N 0.000 description 1
- NNOMNIRQTJWKHE-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-2-(7-methoxy-1H-indol-3-yl)-4-oxobutanoic acid Chemical compound FC1=C(C=CC(=C1)F)C(CC(C(=O)O)C1=CNC2=C(C=CC=C12)OC)=O NNOMNIRQTJWKHE-UHFFFAOYSA-N 0.000 description 1
- OROYXOYGUQDBDU-UHFFFAOYSA-N 4-(2,4-difluorophenyl)-4-oxo-2-(6-phenylmethoxy-1H-indol-3-yl)butanoic acid Chemical compound C(C1=CC=CC=C1)OC1=CC=C2C(=CNC2=C1)C(C(=O)O)CC(=O)C1=C(C=C(C=C1)F)F OROYXOYGUQDBDU-UHFFFAOYSA-N 0.000 description 1
- HGPRRFBGJPLBIQ-UHFFFAOYSA-N 4-(2,4-dimethylphenyl)-4-oxo-2-(1-propylindol-3-yl)butanoic acid Chemical compound CC1=C(C=CC(=C1)C)C(CC(C(=O)O)C1=CN(C2=CC=CC=C12)CCC)=O HGPRRFBGJPLBIQ-UHFFFAOYSA-N 0.000 description 1
- QKRSVVUEVGLGGY-UHFFFAOYSA-N 4-(4-chlorophenyl)-2-(1h-indol-3-yl)-4-oxobutanoic acid Chemical compound C=1NC2=CC=CC=C2C=1C(C(=O)O)CC(=O)C1=CC=C(Cl)C=C1 QKRSVVUEVGLGGY-UHFFFAOYSA-N 0.000 description 1
- VQXMGSAGFQJCAY-UHFFFAOYSA-N 4-(4-fluorophenyl)-2-(1h-indol-3-yl)-4-oxobutanoic acid Chemical compound C=1NC2=CC=CC=C2C=1C(C(=O)O)CC(=O)C1=CC=C(F)C=C1 VQXMGSAGFQJCAY-UHFFFAOYSA-N 0.000 description 1
- GRJZJFUBQYULKL-UHFFFAOYSA-N 4-bromo-1h-indole Chemical compound BrC1=CC=CC2=C1C=CN2 GRJZJFUBQYULKL-UHFFFAOYSA-N 0.000 description 1
- ZWKIJOPJWWZLDI-UHFFFAOYSA-N 4-fluoro-1h-indole Chemical compound FC1=CC=CC2=C1C=CN2 ZWKIJOPJWWZLDI-UHFFFAOYSA-N 0.000 description 1
- 125000001255 4-fluorophenyl group Chemical group [H]C1=C([H])C(*)=C([H])C([H])=C1F 0.000 description 1
- 125000006042 4-hexenyl group Chemical group 0.000 description 1
- 125000006189 4-phenyl benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- MYTGFBZJLDLWQG-UHFFFAOYSA-N 5-chloro-1h-indole Chemical compound ClC1=CC=C2NC=CC2=C1 MYTGFBZJLDLWQG-UHFFFAOYSA-N 0.000 description 1
- IEKPDICCMASELW-UHFFFAOYSA-N 5-ethoxy-1h-indole Chemical compound CCOC1=CC=C2NC=CC2=C1 IEKPDICCMASELW-UHFFFAOYSA-N 0.000 description 1
- ODFFPRGJZRXNHZ-UHFFFAOYSA-N 5-fluoroindole Chemical compound FC1=CC=C2NC=CC2=C1 ODFFPRGJZRXNHZ-UHFFFAOYSA-N 0.000 description 1
- 125000006043 5-hexenyl group Chemical group 0.000 description 1
- DUUGKQCEGZLZNO-UHFFFAOYSA-N 5-hydroxyindoleacetic acid Chemical compound C1=C(O)C=C2C(CC(=O)O)=CNC2=C1 DUUGKQCEGZLZNO-UHFFFAOYSA-N 0.000 description 1
- DWAQDRSOVMLGRQ-UHFFFAOYSA-N 5-methoxyindole Chemical compound COC1=CC=C2NC=CC2=C1 DWAQDRSOVMLGRQ-UHFFFAOYSA-N 0.000 description 1
- IEWMOEZZWOMQHR-UHFFFAOYSA-N 6,6,7,7,7-pentafluoro-2-(1h-indol-3-yl)heptanoic acid Chemical compound C1=CC=C2C(C(CCCC(F)(F)C(F)(F)F)C(=O)O)=CNC2=C1 IEWMOEZZWOMQHR-UHFFFAOYSA-N 0.000 description 1
- YTYIMDRWPTUAHP-UHFFFAOYSA-N 6-Chloroindole Chemical compound ClC1=CC=C2C=CNC2=C1 YTYIMDRWPTUAHP-UHFFFAOYSA-N 0.000 description 1
- SUTWPJHCRAITLU-UHFFFAOYSA-N 6-aminohexan-1-ol Chemical compound NCCCCCCO SUTWPJHCRAITLU-UHFFFAOYSA-N 0.000 description 1
- MAWGHOPSCKCTPA-UHFFFAOYSA-N 6-bromo-1h-indole Chemical compound BrC1=CC=C2C=CNC2=C1 MAWGHOPSCKCTPA-UHFFFAOYSA-N 0.000 description 1
- FPMICYBCFBLGOZ-UHFFFAOYSA-N 6-phenylmethoxy-1h-indole Chemical compound C=1C=C2C=CNC2=CC=1OCC1=CC=CC=C1 FPMICYBCFBLGOZ-UHFFFAOYSA-N 0.000 description 1
- WMYQAKANKREQLM-UHFFFAOYSA-N 7-chloro-1h-indole Chemical compound ClC1=CC=CC2=C1NC=C2 WMYQAKANKREQLM-UHFFFAOYSA-N 0.000 description 1
- FSOPPXYMWZOKRM-UHFFFAOYSA-N 7-methoxy-1h-indole Chemical compound COC1=CC=CC2=C1NC=C2 FSOPPXYMWZOKRM-UHFFFAOYSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- TXOLNEOXCHFNIQ-UHFFFAOYSA-N C(=O)=C(O)C1CCNCC1 Chemical compound C(=O)=C(O)C1CCNCC1 TXOLNEOXCHFNIQ-UHFFFAOYSA-N 0.000 description 1
- HTLUYUVEAGJSDC-UHFFFAOYSA-N C(C)C(CC(C(=O)O)C1=CN(C2=CC=CC=C12)C(=O)OC)CC Chemical compound C(C)C(CC(C(=O)O)C1=CN(C2=CC=CC=C12)C(=O)OC)CC HTLUYUVEAGJSDC-UHFFFAOYSA-N 0.000 description 1
- RSGRXWIUNKWCIL-UHFFFAOYSA-N C1(=CC=CC=C1)C(CC(C)C1=CNC2=CC=CC(=C12)Cl)=O Chemical compound C1(=CC=CC=C1)C(CC(C)C1=CNC2=CC=CC(=C12)Cl)=O RSGRXWIUNKWCIL-UHFFFAOYSA-N 0.000 description 1
- SXRHBIRJFGAVLR-UHFFFAOYSA-N CCCCCCC[n]1c2ccccc2c(CC(OC)=O)c1 Chemical compound CCCCCCC[n]1c2ccccc2c(CC(OC)=O)c1 SXRHBIRJFGAVLR-UHFFFAOYSA-N 0.000 description 1
- RCUNAXHDZAPPSA-UHFFFAOYSA-N CCCc1cc(-c2ccccc2)ccc1 Chemical compound CCCc1cc(-c2ccccc2)ccc1 RCUNAXHDZAPPSA-UHFFFAOYSA-N 0.000 description 1
- NXAWFQKKLGBASV-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCC1CCN(CC1)C(=O)OC(C)(C)C)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCC1CCN(CC1)C(=O)OC(C)(C)C)=O NXAWFQKKLGBASV-UHFFFAOYSA-N 0.000 description 1
- AITRKCNYWCCPJT-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCC1CCN(CC1)C(C)=O)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCC1CCN(CC1)C(C)=O)=O AITRKCNYWCCPJT-UHFFFAOYSA-N 0.000 description 1
- UAYUBLIZPFTJDU-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCC(C(F)(F)F)(F)F)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCC(C(F)(F)F)(F)F)=O UAYUBLIZPFTJDU-UHFFFAOYSA-N 0.000 description 1
- BIFOYLUQXUPLMK-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCCN)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCCN)=O BIFOYLUQXUPLMK-UHFFFAOYSA-N 0.000 description 1
- OLLANMWMVCPCSZ-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCCNC(=O)C1N(CCC1)C(C)=O)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCCCNC(=O)C1N(CCC1)C(C)=O)=O OLLANMWMVCPCSZ-UHFFFAOYSA-N 0.000 description 1
- AYQJTGPEZHNJKR-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCOCCNC(=O)C1N(CCC1)C(C)=O)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCOCCNC(=O)C1N(CCC1)C(C)=O)=O AYQJTGPEZHNJKR-UHFFFAOYSA-N 0.000 description 1
- UXAQRFAIDVPCIH-UHFFFAOYSA-N COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCOCCNC(=O)OC(C)(C)C)=O Chemical compound COC(C(C1=CN(C2=CC=CC=C12)C(=O)OC)CCOCCNC(=O)OC(C)(C)C)=O UXAQRFAIDVPCIH-UHFFFAOYSA-N 0.000 description 1
- LLGBZUKCYOWUPL-UHFFFAOYSA-N COCCc1cc(-c2ccccc2)ccc1 Chemical compound COCCc1cc(-c2ccccc2)ccc1 LLGBZUKCYOWUPL-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical compound NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 229920000089 Cyclic olefin copolymer Polymers 0.000 description 1
- XTHFKEDIFFGKHM-UHFFFAOYSA-N Dimethoxyethane Chemical compound COCCOC XTHFKEDIFFGKHM-UHFFFAOYSA-N 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 102000003951 Erythropoietin Human genes 0.000 description 1
- 108090000394 Erythropoietin Proteins 0.000 description 1
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 108010008212 Integrin alpha4beta1 Proteins 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000283953 Lagomorpha Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- GNMSLDIYJOSUSW-LURJTMIESA-N N-acetyl-L-proline Chemical compound CC(=O)N1CCC[C@H]1C(O)=O GNMSLDIYJOSUSW-LURJTMIESA-N 0.000 description 1
- MBBZMMPHUWSWHV-BDVNFPICSA-N N-methylglucamine Chemical compound CNC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO MBBZMMPHUWSWHV-BDVNFPICSA-N 0.000 description 1
- KULDBTWMMQXNGF-UHFFFAOYSA-N N1C=C(C2=CC=CC=C12)CC(=O)O.COC(CC1=CNC2=CC=CC=C12)=O Chemical compound N1C=C(C2=CC=CC=C12)CC(=O)O.COC(CC1=CNC2=CC=CC=C12)=O KULDBTWMMQXNGF-UHFFFAOYSA-N 0.000 description 1
- 101100030361 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) pph-3 gene Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000282405 Pongo abelii Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 238000006161 Suzuki-Miyaura coupling reaction Methods 0.000 description 1
- OKJPEAGHQZHRQV-UHFFFAOYSA-N Triiodomethane Natural products IC(I)I OKJPEAGHQZHRQV-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- QQIRAVWVGBTHMJ-UHFFFAOYSA-N [dimethyl-(trimethylsilylamino)silyl]methane;lithium Chemical compound [Li].C[Si](C)(C)N[Si](C)(C)C QQIRAVWVGBTHMJ-UHFFFAOYSA-N 0.000 description 1
- FYJKEHKQUPSJDH-UHFFFAOYSA-N [dimethyl-(trimethylsilylamino)silyl]methane;potassium Chemical compound [K].C[Si](C)(C)N[Si](C)(C)C FYJKEHKQUPSJDH-UHFFFAOYSA-N 0.000 description 1
- 229920000122 acrylonitrile butadiene styrene Polymers 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 125000003158 alcohol group Chemical group 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 229910001513 alkali metal bromide Inorganic materials 0.000 description 1
- 229910001516 alkali metal iodide Inorganic materials 0.000 description 1
- 150000001350 alkyl halides Chemical class 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001412 amines Chemical group 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 229940054051 antipsychotic indole derivative Drugs 0.000 description 1
- 239000012300 argon atmosphere Substances 0.000 description 1
- JUHORIMYRDESRB-UHFFFAOYSA-N benzathine Chemical compound C=1C=CC=CC=1CNCCNCC1=CC=CC=C1 JUHORIMYRDESRB-UHFFFAOYSA-N 0.000 description 1
- 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 1
- 125000001231 benzoyloxy group Chemical group C(C1=CC=CC=C1)(=O)O* 0.000 description 1
- 125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000005385 borate glass Substances 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 150000003842 bromide salts Chemical class 0.000 description 1
- RDHPKYGYEGBMSE-UHFFFAOYSA-N bromoethane Chemical compound CCBr RDHPKYGYEGBMSE-UHFFFAOYSA-N 0.000 description 1
- GZUXJHMPEANEGY-UHFFFAOYSA-N bromomethane Chemical compound BrC GZUXJHMPEANEGY-UHFFFAOYSA-N 0.000 description 1
- 125000004744 butyloxycarbonyl group Chemical group 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000035606 childbirth Effects 0.000 description 1
- RGFRUPDYJJSLHS-UHFFFAOYSA-N chloroform;methyl acetate Chemical compound ClC(Cl)Cl.COC(C)=O RGFRUPDYJJSLHS-UHFFFAOYSA-N 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 229940015047 chorionic gonadotropin Drugs 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 210000001771 cumulus cell Anatomy 0.000 description 1
- VZFUCHSFHOYXIS-UHFFFAOYSA-N cycloheptane carboxylic acid Natural products OC(=O)C1CCCCCC1 VZFUCHSFHOYXIS-UHFFFAOYSA-N 0.000 description 1
- ISQVBYGGNVVVHB-UHFFFAOYSA-N cyclopentylmethanol Chemical compound OCC1CCCC1 ISQVBYGGNVVVHB-UHFFFAOYSA-N 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- ZFTFAPZRGNKQPU-UHFFFAOYSA-N dicarbonic acid Chemical compound OC(=O)OC(O)=O ZFTFAPZRGNKQPU-UHFFFAOYSA-N 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical group C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- ODCCJTMPMUFERV-UHFFFAOYSA-N ditert-butyl carbonate Chemical compound CC(C)(C)OC(=O)OC(C)(C)C ODCCJTMPMUFERV-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940105423 erythropoietin Drugs 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 229960004756 ethanol Drugs 0.000 description 1
- 125000003754 ethoxycarbonyl group Chemical group C(=O)(OCC)* 0.000 description 1
- JTUFDBBKQGXLAV-UHFFFAOYSA-N ethyl 2-(7-hydroxynaphthalen-1-yl)acetate Chemical compound C1=C(O)C=C2C(CC(=O)OCC)=CC=CC2=C1 JTUFDBBKQGXLAV-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000021267 infertility disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-M iodide Chemical compound [I-] XMBWDFGMSWQBCA-UHFFFAOYSA-M 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- UBJFKNSINUCEAL-UHFFFAOYSA-N lithium;2-methylpropane Chemical compound [Li+].C[C-](C)C UBJFKNSINUCEAL-UHFFFAOYSA-N 0.000 description 1
- WGOPGODQLGJZGL-UHFFFAOYSA-N lithium;butane Chemical compound [Li+].CC[CH-]C WGOPGODQLGJZGL-UHFFFAOYSA-N 0.000 description 1
- IVSZLXZYQVIEFR-UHFFFAOYSA-N m-xylene Chemical group CC1=CC=CC(C)=C1 IVSZLXZYQVIEFR-UHFFFAOYSA-N 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 241001515942 marmosets Species 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229940098779 methanesulfonic acid Drugs 0.000 description 1
- 125000001160 methoxycarbonyl group Chemical group [H]C([H])([H])OC(*)=O 0.000 description 1
- JWLZLLFFFQKDHR-UHFFFAOYSA-N methyl 2-(1-methylindol-3-yl)acetate Chemical compound C1=CC=C2C(CC(=O)OC)=CN(C)C2=C1 JWLZLLFFFQKDHR-UHFFFAOYSA-N 0.000 description 1
- ZJNRJAAVXVHVFU-UHFFFAOYSA-N methyl 2-(5-butoxy-1h-indol-3-yl)acetate Chemical compound CCCCOC1=CC=C2NC=C(CC(=O)OC)C2=C1 ZJNRJAAVXVHVFU-UHFFFAOYSA-N 0.000 description 1
- UZOYTCVZJBYXIA-UHFFFAOYSA-N methyl 2-(5-methoxy-1h-indol-3-yl)acetate Chemical compound C1=C(OC)C=C2C(CC(=O)OC)=CNC2=C1 UZOYTCVZJBYXIA-UHFFFAOYSA-N 0.000 description 1
- XIORGLOOXQRGOC-UHFFFAOYSA-N methyl 2-[5-[(3,5-dimethoxyphenyl)methoxy]-1h-indol-3-yl]acetate Chemical compound C1=C2C(CC(=O)OC)=CNC2=CC=C1OCC1=CC(OC)=CC(OC)=C1 XIORGLOOXQRGOC-UHFFFAOYSA-N 0.000 description 1
- FSIXUALGERODRW-UHFFFAOYSA-N methyl 3-(1-methoxy-1-oxo-4-phenylbutan-2-yl)indole-1-carboxylate Chemical compound C=1N(C(=O)OC)C2=CC=CC=C2C=1C(C(=O)OC)CCC1=CC=CC=C1 FSIXUALGERODRW-UHFFFAOYSA-N 0.000 description 1
- YGMGQCMNPOHERX-UHFFFAOYSA-N methyl 3-(3-cyclopentyl-1-methoxy-1-oxopropan-2-yl)indole-1-carboxylate Chemical compound C=1N(C(=O)OC)C2=CC=CC=C2C=1C(C(=O)OC)CC1CCCC1 YGMGQCMNPOHERX-UHFFFAOYSA-N 0.000 description 1
- ALRRINVBHFXUSK-UHFFFAOYSA-N methyl 3-(4-cyclopentyl-1-methoxy-1-oxobutan-2-yl)indole-1-carboxylate Chemical compound C=1N(C(=O)OC)C2=CC=CC=C2C=1C(C(=O)OC)CCC1CCCC1 ALRRINVBHFXUSK-UHFFFAOYSA-N 0.000 description 1
- OLTFCXODALHLFC-UHFFFAOYSA-N methyl 3-(4-ethyl-1-methoxy-1-oxohexan-2-yl)indole-1-carboxylate Chemical compound C1=CC=C2C(C(C(=O)OC)CC(CC)CC)=CN(C(=O)OC)C2=C1 OLTFCXODALHLFC-UHFFFAOYSA-N 0.000 description 1
- XFAKNYUXAFIUTE-UHFFFAOYSA-N methyl 3-[1-methoxy-1-oxo-4-(3-phenylphenyl)butan-2-yl]indole-1-carboxylate Chemical compound C=1N(C(=O)OC)C2=CC=CC=C2C=1C(C(=O)OC)CCC(C=1)=CC=CC=1C1=CC=CC=C1 XFAKNYUXAFIUTE-UHFFFAOYSA-N 0.000 description 1
- BTQIYNQKMBXPKW-UHFFFAOYSA-N methyl 3-[1-methoxy-2-methyl-8-[(2-methylpropan-2-yl)oxycarbonylamino]-1-oxooctan-2-yl]indole-1-carboxylate Chemical compound C1=CC=C2N(C(=O)OC)C=C(C(C)(CCCCCCNC(=O)OC(C)(C)C)C(=O)OC)C2=C1 BTQIYNQKMBXPKW-UHFFFAOYSA-N 0.000 description 1
- DHSTXLGEZDITRP-UHFFFAOYSA-N methyl 3-[4-(2-aminoethoxy)-1-methoxy-1-oxobutan-2-yl]indole-1-carboxylate Chemical compound C1=CC=C2C(C(CCOCCN)C(=O)OC)=CN(C(=O)OC)C2=C1 DHSTXLGEZDITRP-UHFFFAOYSA-N 0.000 description 1
- XMJHPCRAQCTCFT-UHFFFAOYSA-N methyl chloroformate Chemical compound COC(Cl)=O XMJHPCRAQCTCFT-UHFFFAOYSA-N 0.000 description 1
- DVSDBMFJEQPWNO-UHFFFAOYSA-N methyllithium Chemical compound C[Li] DVSDBMFJEQPWNO-UHFFFAOYSA-N 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 208000012268 mitochondrial disease Diseases 0.000 description 1
- 230000004065 mitochondrial dysfunction Effects 0.000 description 1
- 230000004898 mitochondrial function Effects 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- XONPDZSGENTBNJ-UHFFFAOYSA-N molecular hydrogen;sodium Chemical compound [Na].[H][H] XONPDZSGENTBNJ-UHFFFAOYSA-N 0.000 description 1
- PYLWMHQQBFSUBP-UHFFFAOYSA-N monofluorobenzene Chemical compound FC1=CC=CC=C1 PYLWMHQQBFSUBP-UHFFFAOYSA-N 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 125000003136 n-heptyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000001298 n-hexoxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 1
- 125000004433 nitrogen atom Chemical group N* 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- KDLHZDBZIXYQEI-UHFFFAOYSA-N palladium Substances [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 1
- 125000004817 pentamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 description 1
- 125000002255 pentenyl group Chemical group C(=CCCC)* 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- UYWQUFXKFGHYNT-UHFFFAOYSA-N phenylmethyl ester of formic acid Natural products O=COCC1=CC=CC=C1 UYWQUFXKFGHYNT-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920000306 polymethylpentene Polymers 0.000 description 1
- 239000011116 polymethylpentene Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229960002429 proline Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000005297 pyrex Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 235000012045 salad Nutrition 0.000 description 1
- 108010038379 sargramostim Proteins 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000005368 silicate glass Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- WRIKHQLVHPKCJU-UHFFFAOYSA-N sodium bis(trimethylsilyl)amide Chemical compound C[Si](C)(C)N([Na])[Si](C)(C)C WRIKHQLVHPKCJU-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 150000003459 sulfonic acid esters Chemical class 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- LFKDJXLFVYVEFG-UHFFFAOYSA-N tert-butyl carbamate Chemical compound CC(C)(C)OC(N)=O LFKDJXLFVYVEFG-UHFFFAOYSA-N 0.000 description 1
- 125000005931 tert-butyloxycarbonyl group Chemical group [H]C([H])([H])C(OC(*)=O)(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- ITMCEJHCFYSIIV-UHFFFAOYSA-N triflic acid Chemical compound OS(=O)(=O)C(F)(F)F ITMCEJHCFYSIIV-UHFFFAOYSA-N 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229960001005 tuberculin Drugs 0.000 description 1
- 238000007039 two-step reaction Methods 0.000 description 1
- 238000005292 vacuum distillation Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/12—Radicals substituted by oxygen atoms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
- A61K31/403—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil condensed with carbocyclic rings, e.g. carbazole
- A61K31/404—Indoles, e.g. pindolol
- A61K31/405—Indole-alkanecarboxylic acids; Derivatives thereof, e.g. tryptophan, indomethacin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
- C07D209/18—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
- C07D209/20—Radicals substituted by carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals substituted additionally by nitrogen atoms, e.g. tryptophane
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
- C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
- C07D401/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0604—Whole embryos; Culture medium therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0609—Oocytes, oogonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/061—Sperm cells, spermatogonia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Gynecology & Obstetrics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Endocrinology (AREA)
- Epidemiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Indole Compounds (AREA)
Abstract
Description
〔1〕以下の一般式(I0)、一般式(II)、及び一般式(III)で表される化合物、並びに、R3がOHのときそれらの生理学的に許容される塩からなる群から選択される1種又は2種以上の化合物を含む、哺乳類精子の受精機能増強剤。
〔2〕化合物が、以下の式(I−2)で表される化合物若しくはその生理学的に許容される塩である、上記〔1〕に記載の受精機能増強剤。
式(I−2);
〔4〕化合物が、以下の式(I−2)で表される化合物若しくはその生理学的に許容される塩である、上記〔3〕に記載の増強方法。
式(I−2);
〔6〕採取された少なくとも1つの哺乳類精子と、採取された少なくとも1つの哺乳類卵子とを、以下の一般式(I0)、一般式(II)、及び一般式(III)で表される化合物、並びに、R3がOHのときそれらの生理学的に許容される塩からなる群から選択される1種又は2種以上の化合物を含む培養液中で培養する工程(b−2)を含む、哺乳類受精卵の作製方法。
〔7〕化合物が、以下の式(I−2)で表される化合物若しくはその生理学的に許容される塩である、上記〔6〕に記載の作製方法。
式(I−2);
以下に示す化合物の合成方法に用いる合成原料、反応試薬等は一般的な市販品である。また、反応溶媒、反応温度に関して特に記載のない場合は、通常その反応に利用される溶媒、温度で反応が行われる。また、反応は、アルゴン若しくは乾燥させた窒素雰囲気下で行われる。
4−フェニル−2−(4−クロロ−1H−インドール−3−イル)−4−オキソ−ブタン(化合物#1)は、インドールの代わりに4−クロロインドールを用いて、後述する化合物#20の合成方法により合成した。
4−(4−クロロフェニル)−2−(1H−インドール−3−イル)−4−オキソ−ブタン酸(化合物#2)及び3−(1H−インドール−3−イル)−1−オキソ−1−フェニル−ブタン(化合物#3)は、Sayed,G. H. et al, “Synthesis and reactions of some β-aroyl-α-(indol-3-yl)propionic acids” Journal of the Chemical Society of Pakistan,7(4), 263-72; 1985の記載の方法に従って合成した。
トランス−4−(4−フルオロフェニル)−4−オキソ−2−ブテン酸
トランス−4−(2,4−ジフルオロフェニル)−4−オキソ−2−ブテン酸
for 330.0942 (C18H14F2NO3).
トランス−4−(2,4−ジメチルフェニル)−4−オキソ−2−ブテン酸
インドール−3−酢酸(2.00g,11.42mmol)をメタノール(40ml)に溶かし、そこに塩化アセチル(0.5ml,6.688mmol)を一滴ずつ滴下し、室温で2時間撹拌した。TLCで反応終了を確認した後、飽和重曹水溶液を加え、反応を停止させ、酢酸エチル(50ml)で3回抽出した。有機層を飽和食塩水で2回洗浄し、無水硫酸ナトリウムで脱水した。溶媒を減圧留去した後、シリカゲルカラムクロマトグラフィー(ヘキサン:酢酸エチル=7:3)で精製し、インドール−3−酢酸 メチルエステルを得た。(2.14g,収率99%):1H NMR(400MHz,CDCl3):δ 8.13(s,1H),6.97(s,1H),7.59(d,J=7.7Hz,1H),7.23(d,J=7.9Hz,1H),7.10−7.19(m,2H),3.67(s,3H),3.76(s,2H);13C NMR(100MHz,CDCl3):δ 172.3,136.0,127.1,123.2,122.0,119.5,118.6,111.2,108.0,51.9,31.0;IR(neat):3410,1730,1458,1435,1337,1164,1095,1011cm−1;EI−MS m/z 189 [M]+.
インドール−3−酢酸メチル(2.00g,10.57mmol)をジクロロメタン(30ml)に溶かし、そこにヨウ化テトラブチルアンモニウム(TBAI,30.0mg,0.081mmol)、30%水酸化ナトリウム水溶液(24ml)を加え、0℃に冷却した。反応液に塩化ギ酸メチル(1.96g,20.73mmol)を加え、0℃で2時間撹拌した。TLCで反応終了を確認した後、6N塩酸を加え、反応を停止させた。水(50ml)を加え、クロロホルム(50ml)で3回抽出し、有機層を飽和食塩水で2回洗浄し、無水硫酸ナトリウムで脱水した。溶媒を減圧留去した後、シリカゲルカラムクロマトグラフィー(ヘキサン:酢酸エチル=8:2)で精製し、N−メトキシカルボニルインドール−3−酢酸メチルを得た。(2.26g,収率87%):1H NMR(400MHz,CDCl3):δ 8.18(d,J=7.0Hz,1H),7.59(s,1H),7.53(d,J=7.7Hz,1H),7.35(t,J=7.5Hz,1H),7.27(t,J=7.4Hz,1H),4.00(s,3H),3.72(s,3H),3.71(s,2H);13C NMR(100MHz,CDCl3):δ 171.1,151.1,135.2,129.9,124.6,123.8,122.8,118.9,115.0,113.8,53.5,51.9,30.6;IR(neat):1746,1455,1382,1258,1164,1089,1018cm−1;EI−MS: m/z 247 [M]+.
2−(N−tert−ブトキシカルボニル−4−ピペリジニル)エタノール
α−4−アミノブチル−N−メトキシカルボニル−3−インドール酢酸 メチルエステル
(m,2H);13C NMR(100MHz,CDCl3):δ 173.8,171.0,170.8,151.1,135.3,129.2,124.6,122.9,122.8,119.2,119.1,115.0,59.4,53.6,51.9,48.1,42.3,38.9,31.5,29.0,27.2,24.8,24.7,22.3;FAB−MS:m/z 458[M+H]+.
α−[2−(2−アミノエトキシ)−エチル]−N−メトキシカルボニル−3−インドール酢酸 メチルエステル
α−(N−tert−ブトキシカルボニル−6−アミノ−1−ヘキシル)−α−(1−ナフチル)−酢酸 メチルエステル
N−tert−ブトキシカルボニル−6−アミノ−1−ヘキサノール
8.19(d,J=6.3Hz,1H),7.52(d,J=7.9Hz,1H),7.48(s,1H),7.32(t,J=7.5Hz,1H),7.21(t,J=7.5Hz,1H),4.54(s,1H),4.03(s,3H),3.62(s,3H),3.06(m,2H),2.04−2.12(m,2H),1.61(s,3H),1.17−1.43(m,17H);13C NMR(100MHz,CDCl3):δ 176.3,155.9,151.3,135.8,128.6,124.9,124.5,122.8,122.0,120.0,115.2,78.9,53.7,52.1,45.5,40.4,37.2,29.9,29.5,28.3,26.5,24.2,22.5;FAB−MS:m/z 460[M]+.
2−(N−tert−ブトキシカルボニル−2−アミノエトキシ)−エタノール
N−tert−ブトキシカルボニル−4−アミノ−1−ブタノール
2−エチル−1−ヨードブタン
3−メチル−1−ヨードペンタン
2−メチル−1−ヨードペンタン
4−フェニル−2−(1H−インドール−3−イル)−4−オキソ−ブタン酸
(化合物#20)
IR:(neat):3400,3055,1711,1677,1453cm−1;HRFAB−MS found m/z 294.1143[M+H]+, calcd for 294.1130(C18H16NO3).
4,4,5,5,5−ペンタフルオロ−1−ヨードペンタン
3−(2−ヒドロキシ−1−エチル)−1,1’−ビフェニル
355[M]+.
α−(2−フェニル−1−エチル)−1−メトキシカルボニル−3−インドール酢酸 メチルエステル
2−シクロペンチル−1−ヨードエタン
シクロペンチルヨードメタン
に記載の方法に従って合成した。
N−メチル−3−インドール酢酸 メチルエステル
N−エチル−3−インドール酢酸 メチルエステル
N−プロピル−3−インドール酢酸 メチルエステル
N−ブチル−3−インドール酢酸 メチルエステル
N−ヘキシル−3−インドール酢酸 メチルエステル
N−ヘプチル−3−インドール酢酸 メチルエステル
α−(7−ブトキシ−1−ナフタレニル)−酢酸 エチルエステル
認した後、6N塩酸を加え酸性(pH=3〜4)にし、溶媒を減圧留去した。水(5ml)を加え、酢酸エチル(10ml)で3回抽出した。有機層を飽和食塩水で2回洗浄し、無水硫酸ナトリウムで脱水した。溶媒を減圧留去した後、シリカゲルカラムクロマトグラフィー(クロロホルム:メタノール=9:1)で精製しα−(7−ブトキシ−1−ナフタレニル)−酢酸(化合物#33)を得た。(67mg,収率98%):融点102〜104℃;1H NMR(400MHz,CDCl3):δ 7.75(d,J=8.9Hz,1H),7.71(d,J=8.1Hz,1H),7.34(d,J=6.9Hz,1H),7.26(dd,J=8.1,6.9Hz,1H),7.23(d,J=2.0Hz,1H),7.16(q,J=8.9,2.0Hz,1H),4.05(t,J=6.5Hz,2H),4.00(s,2H),1.51(m,2H),1.80(m,2H),0.98(t,J=7.4Hz,3H);13C NMR(100MHz,CDCl3):δ 177.6,157.6,133.2,130.2,129.1,128.6,127.9(2C),123.0,118.7,103.1,67.7,39.2,31.2,19.3,13.8;IR(neat):3021,2931,1699,1457,1138cm−1;HREI−MS found m/z 258.1268[M]+,calcd for 258.1256(C16H18O3).
α−(7−ペントキシ−1−ナフタレニル)−酢酸 エチルエステル
5−(3,5−ジメトキシベンジルオキシ)−3−インドール酢酸 メチルエステル
5−メトキシ−3−インドール酢酸 メチルエステル
5−エトキシ−3−インドール酢酸 メチルエステル
5−(1−プロポキシ)−3−インドール酢酸 メチルエステル
5−(1−ブトキシ)−3−インドール酢酸 メチルエステル
1H-NMR(400MHz, CDCl3) δ 10.31 (s, 1H), 8.01 (m, 1H), 7.47 (dd, J=8.8, 5.2, 1H),7.34 (d, J=2.0, 2H), 7.12-7.20 (m, 3H), 6.92 (td, J=9.6, 2.4, 1H), 4.54 (dd, J=10.4, 4.0, 1H), 4.01 (ddd, J=18.8, 10.8, 3.2, 1H), 3.38 (td, J=18.8, 3.2, 1H);
13C-NMR (100MHz, CDCl3) δ 195.18, 174.85, 166.28 JC-F(dd, 254, 13 Hz), 163.42 JC-F (dd, 254, 13 Hz), 161.74, 159.4, 137.54 JC-F (d, 13 Hz), 133.47 JC-F(dd, 11,3 Hz), 123.35 JC-F(d, 4Hz), 123.02 JC-F (dd, 13, 4 Hz), 120.97 JC-F (d, 11 Hz), 113.51, 112.94 JC-F (dd, 10, 2 Hz) , 108.27 JC-F (d, 24 Hz), 105.59 JC-F (t, 27 Hz), 98.27 JC-F (d, 26 Hz), 46.68 JC-F (d, 7Hz) ,38.47;
FAB-MS m/z = 348 [M+H]+
1H-NMR(400MHz, acetone-d6) δ10.34 (s, 1H), 8.02 (m, 1H), 7.47 (dd, J=10.4, 2.8,1H), 7.39-7.43 (m, 2H), 7.13-7.21 (m, 2H), 6.93 (td, J=9.2 , 2.8, 1H), 4.52 (dd, J=10.4, 3.6, 1H), 4.03 (ddd, 18.4, 10.8, 3.6, 1H), 3.40 (td, 18.4, 3.6, 1H); 13C-NMR (100MHz, acetone-d6) δ 195.17, 174.86, 166.52 JC-F(dd, 254, 13 Hz), 163.58 JC-F(dd, 254, 13 Hz), 159.52, 157.21, 134.25, 133.46 JC-F (dd, 11, 4 Hz), 127.66 JC-F(d, 11 Hz), 123.01 JC-F (dd, 10, 4 Hz), 113.42 JC-F (d, 5 Hz), 113.28 JC-F(d, 10 Hz), 113.51 JC-F (dd, 21, 4 Hz), 110.56 JC-F(d, 27 Hz), 105.61 JC-F (t, 27 Hz), 104.65 JC-F (d, 24 Hz), 46.68 JC-F (d, 8Hz), 38.48;
FAB-MS m/z = 348 [M+H]+
1H-NMR(400MHz, acetone-d6) δ10.55 (s, 1H), 8.01 (m, 1H), 7.75 (d, J=8.4, 1H), 7.45 (d, J=2.8, 1H), 7.06-7.14 (m, 4H), 7.08 (t. J=7.6, 1H), 4.57 (dd, J=10.4 , 3.6, 1H), 4.03 (ddd, 18.4, 10.8, 3.6, 1H), 3.41 (td, 18.4, 3.6, 1H);
13C-NMR(100MHz, acetone-d6) δ195.06, 74.67, 166.53, JC-F(dd, 252, 12 Hz), 163.63 JC-F(dd, 252, 12 Hz), 134.45, 133.46 JC-F(dd, 11, 5 Hz), 129.25, 124.97, 122.98 JC-F(dd, 13, 4 Hz), 121.91, 120.84, 119.03, 117.22, 114.73, 112.99 JC-F(dd, 21, 3 Hz), 105.61 JC-F(t, 27 Hz), 46.71 JC-F (d, 8 Hz) , 38.50;
FAB-MS m/z = 364 [M+H]+
1H-NMR(400MHz, acetone-d6) δ10.45 (s, 1H), 8.02 (q, J=8.3, 1H), 7.80 (d, J=1.6,1H), 7.44 (m, 2H), 7.11-7.22 (m, 3H), 4.54 (dd, J=10.4, 3.8, 1H), 4.01 (ddd, J=18.7, 10.7, 3.2, 1H), 3.41 (td, J=18.7, 3.2, 1H);
13C-NMR (100MHz, acetone-d6) δ195.10, 174.76, 166.52 JC-F(dd, 253, 12Hz), 163.62 JC-F(dd, 253, 12 Hz), 136.06, 133.44 JC-F(dd, 12, 4 Hz), 128.47, 125.62, 122.96 JC-F(dd, 13, 4 Hz), 122.48, 119.33, 113.77, 113.12, 112.96 JC-F(dd, 22, 3 Hz), 105.61 JC-F(t, 27 Hz), 46.72 JC-F (d, 8 Hz), 38.35;
FAB-MS m/z = 364 [M+H]+
1H-NMR(400MHz, acetone-d6) δ10.24 (s, 1H), 8.01 (m, 1H), 7.77 (d, J=8.4, 1H), 7.42 (d, J=8.0, 1H), 7.21〜7.03 (m, 4H), 4.57 (dd, J=10.8, 3.6, 1H), 4.03 (ddd 18.8, 10.8, 3.2, 1H), 3.38 (td, 18.8, 3.2, 1H);
13C-NMR (100MHz, acetone-d6) δ195.28, 174.97, 166.55 JC-F(dd, 254, 12 Hz), 163.62 JC-F(dd, 254, 12 Hz), 137.646, 137.49, 133.46 JC-F (dd, 11, 4 Hz), 127.37, 123.70, 123.54, 123.05 JC-F(dd, 13, 4 Hz), 122.39, 119.88, 119.79, 112.95 JC-F(dd, 22, 4 Hz), 105.58 JC-F (t, 26 Hz), 46.95 JC-F (d, 8 Hz), 38.47;
FAB-MS m/z = 364 [M+H]+
1H-NMR(400MHz, acetone-d6) δ10.10 (s, 1H), 8.01 (m, 1H), 7.54 (s, 1H), 7.20-7.31 (m, 2H), 7.13-7.20 (m, 1H), 7.96 (d, J=6.8, 1H), 4.53 (dd, J=10.6, 3.6, 1H), 4.01 (ddd 18.8, 10.6, 3.2, 1H), 3.36 (td, 18.8, 3.2, 1H), 2.40 (s, 3H);
13C-NMR (100MHz, acetone-d6) δ195.33, 175.08, 166.54 JC-F(dd, 254, 12 Hz), 163.49 JC-F(dd, 254, 12 Hz), 136.01, 133.45 JC-F(dd, 11, 4 Hz), 128.59, 127.63, 124.02, 123.72, 123.05 JC-F (dd, 13, 4 Hz), 119.43, 112.94 JC-F(dd, 22, 4 Hz), 112.74, 112.03, 105.59 JC-F(t, 26 Hz), 47.01 JC-F(d, 7 Hz), 38.45, 21.64;
FAB-MS m/z = 344 [M+H]+
1H-NMR(400MHz, acetone-d6) δ 8.00 (m, 1H), 7.75 (d, J=7.6, 1H) , 7.37 (d, J=8.4, 1H), 7.12-7.22 (m, 4H), 7.07 (t, J=7.6, 1H), 4.54 (dd, J=10.8, 3.6, 1H), 4.00 (ddd, 18.8, 10.4, 3.6, 1H), 3.79 (s, 3H), 3.36 (td, 18.8, 3.6, 1H);
13C-NMR (100MHz, acetone-d6) δ195.22, 174.94, 166.52 JC-F(dd, 253, 13 Hz), 163.57 JC-F(dd, 253, 13 Hz), 138.08, 133.46 JC-F(dd, 11, 4 Hz), 128.00, 127.79, 123.03 JC-F(dd, 13, 4 Hz), 122.36, 120.07, 119.72, 112.94 JC-F(d, 22, 4 Hz), 112.33, 110.32, 105.60 JC-F(t, 27 Hz), 47.01 JC-F(d, 8 Hz), 38.36, 32.72;
FAB-MS m/z = 344 [M+H]+
1H-NMR(400MHz, acetone-d6) δ10.26 (s, 1H), 8.01 (m, 1H), 7.35 (d, J=8.0, 1H),7.28 (d, J=2.8, 1H), 7.12-7.20 (m, 2H), 6.98 (t, J=7.8, 1H), 6.67 (d, J=7.8, 1H),4.54 (dd, J=10.8, 3.6, 1H), 4.03 (ddd 18.8, 10.6, 3.3, 1H), 3.92 (s, 3H), 3.36(td, 18.6, 3.2, 1H);
13C-NMR (100MHz, acetone-d6) δ1195.28, 174.98, 166.54 JC-F(dd, 252, 12 Hz), 163.48 JC-F(dd, 252, 12 Hz), 147.35, 133.44 JC-F(dd, 11, 4 Hz), 128.81, 127.83, 123.22, 123.05 JC-F (dd, 13, 4 Hz) , 120.43, 113.76, 112.94 JC-F(dd, 22, 4 Hz), 112.7, 105.59 JC-F(t, 27 Hz), 102.52, 55.52, 46.97 JC-F(d, 8 Hz) , 38.59;
FAB-MS m/z = 360 [M+H]+
1H-NMR(400MHz, acetone-d6) δ10.09 (s, 1H), 8.02 (m, 1H), 7.27-7.31 (m, 3H), 7.13-7.20 (m, 2H), 6.79 (dd, J=8.8, 2.4, 1H), 4.52 (dd, J=10.8, 3.6, 1H), 4.00 (ddd, 18.8, 10.4, 3.4, 1H), 3.80 (s, 3H), 3.38 (td, 18.6, 3.4, 1H;
13C-NMR (100MHz, acetone-d6) δ195.35, 175.03, 166.53, JC-F(dd, 253, 12 Hz), 163.61 JC-F(dd, 253, 12 Hz), 154.84, 133.45 JC-F(dd, 11, 4 Hz), 132.72, 127.78, 124.22, 123.04 JC-F (dd, 12, 4 Hz), 112.95 JC-F (dd, 22, 4 Hz), 112.96, 112.68, 105.61 JC-F (t, 26 Hz), 101.58, 55.79, 46.9 JC-F(d, 8 Hz), 38.67;
FAB-MS m/z = 360 [M+H]+
1H-NMR(400MHz, acetone-d6) δ 10.0 4(s, 1H), 8.01 (m, 1H), 7.65 (d, J=8.8, 1H), 7.48 (d, J-7.6, 2H), 7.38 (t, J=7.2, 2H), 7.31 (m, 1H), 7.13-7.20 (m, 3H), 7.03 (d, J=2.0, 1H), 6.83 (dd, J=8.4 , 2.0, 1H), 4.51 (dd, J=10.4 , 3.6, 1H), 4.01 (ddd, 18.1, 10.4, 3.3, 1H), 3.36 (td, 18.1, 3.3, 1H);
13C-NMR (100MHz, acetone-d6) δ195.29, 175.00, 166.54 JC-F(dd, 254, 12 Hz), 163.52 JC-F(dd, 254, 12 Hz), 156.33, 138.85, 138.45, 133.45, JC-F (dd, 11, 4 Hz), 129.17, 128.37, 128.23, 123.01 JC-F(dd, 13, 4 Hz), 122.49, 121.97, 120.54, 113.24,113.06 JC-F (dd, 21, 4 Hz), 110.73, 105.59 JC-F(t, 27 Hz), 96.89, 70.70, 46.94 JC-F(d, 8 Hz), 38.57;
FAB-MS m/z = 436 [M+H]+
(E)−4−(2,4−ジフルオロフェニル)−4−オキソ−2−ブテン酸
1H-NMR(400MHz, acetone-d6) δ 7.98 (m, 1H), 7.72 (dd, J=15.6 , 3.6, 1H), 7.20-7.28 (m, 2H), 6.75 (d, J=15.6, 2H);
13C-NMR(100MHz, acetone-d6) δ187.13, 166.86 JC-F(dd, 254, 12 Hz), 166.35, 163.33 JC-F(dd, 254, 12 Hz), 139.95 JC-F(d, 7 Hz), 133.98 JC-F (dd, 66, 59 Hz), 132.91, 123.22 JC-F(d, 9 Hz), 113.33 JC-F(dd, 22 , 3 Hz), 105.76, JC-F (t, 22 Hz)
実施例1で合成した本件化合物群(化合物#5)を用い、以下に示す方法に従って、哺乳類精子の受精機能増強効果を確認した。なお、精子の前培養及び媒精は、5%CO2/20%O2、37℃条件下で行った。
〔1〕メスのC57/B6マウスに、妊馬血清性腺刺激ホルモン(PMSG;pregnant mare serum gonadotropin)を腹腔内投与し、PMSG投与48時間後にヒト絨毛性性腺刺激ホルモン(hCG;Human chorionic gonadotropin)することにより過排卵を誘起した。hCG投与5〜17時間後にマウスを安楽死させ、卵管を回収した。
〔2〕オスのC57/B6マウスを安楽死させた後、精巣上体の尾部を摘出し、回収した。
〔3〕100μLの4種類の培養液(0.0001%のDMSOを含む媒精用培養液[TYH培養液、LSIメディエンス社製]、及び1μMの化合物#5を含むTYH培養液、並びに、10μMのFCCP(Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone)を含むTYH培養液、及び10μMのFCCPと1μMの化合物#5とを含むTYH培養液)からなる滴(ドロップ)をそれぞれ調製し、その上をミネラルオイル(シグマ−アルドリッチ社製)で覆った。上記回収した精巣上体の尾部を、上記ミネラルオイル中に移し、ピンセットで把持した状態で1mLの26G針付きツベルクリン用シリンジ(ニプロ社製)にて精巣上体管に傷をつけ、得られた精子塊を、ミネラルオイル中の上記4種類の培養液中にそれぞれ移し、媒精前の培養(前培養)を1時間行った。
〔4〕上記4種類の培養液中で前培養した精子3μLを、マイクロピペッター(エッペンドルフ社製、ドイツ)を用いて、卵丘細胞−卵子複合体(COC;cumulus oocyte complex)を含むTYH培養液のドロップ200μL中にそれぞれ移し、その上をミネラルオイル(シグマ−アルドリッチ社製)で覆い、媒精を行った。
〔5〕媒精24〜28時間後に2細胞期卵の有無を観察し、2細胞期卵が観察された場合、受精したと評価し、受精率を測定した。
化合物#5の溶媒であるDMSOを含むTYH培養液、すなわち、化合物#5不含の培養液中で前培養した精子を用いて媒精を行うと、受精率40%であったのに対して、化合物#5含有培養液中で前培養した精子を用いて媒精を行うと、受精率は100%であった(表1参照)。
この結果は、本件化合物群(化合物#5)は、精子の受精機能を賦活化(増強)する作用を有することを示している。
この結果は、本件化合物群(化合物#5)は、低下した精子の受精機能(例えば、ATPを利用した前進運動)を改善する作用を有することを示している。
Claims (7)
- 以下の一般式(I0)、一般式(II)、及び一般式(III)で表される化合物、並びに、R3がOHのときそれらの生理学的に許容される塩からなる群から選択される1種又は2種以上の化合物を含む、哺乳類精子の受精機能増強剤。
- 採取された少なくとも1つの哺乳類精子を、以下の一般式(I0)、一般式(II)、及び一般式(III)で表される化合物、並びに、R3がOHのときそれらの生理学的に許容される塩からなる群から選択される1種又は2種以上の化合物を含む生理的水溶液中に移す工程(a)を含む、哺乳類精子の受精機能の増強方法。
- 請求項3又は4に記載の増強方法により得られた哺乳類精子を、採取された少なくとも1つの哺乳類卵子を含む培養液中で培養する工程(b−1)を含む、哺乳類受精卵の作製方法。
- 採取された少なくとも1つの哺乳類精子と、採取された少なくとも1つの哺乳類卵子とを、以下の一般式(I0)、一般式(II)、及び一般式(III)で表される化合物、並び
に、R3がOHのときそれらの生理学的に許容される塩からなる群から選択される1種又は2種以上の化合物を含む培養液中で培養する工程(b−2)を含む、哺乳類受精卵の作製方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2018107827 | 2018-06-05 | ||
JP2018107827 | 2018-06-05 | ||
PCT/JP2019/022082 WO2019235455A1 (ja) | 2018-06-05 | 2019-06-04 | 精子の受精機能増強剤 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2019235455A1 true JPWO2019235455A1 (ja) | 2021-06-17 |
JP7373804B2 JP7373804B2 (ja) | 2023-11-06 |
Family
ID=68769924
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2020523109A Active JP7373804B2 (ja) | 2018-06-05 | 2019-06-04 | 精子の受精機能増強剤 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20220106270A1 (ja) |
JP (1) | JP7373804B2 (ja) |
WO (1) | WO2019235455A1 (ja) |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014080640A1 (ja) | 2012-11-26 | 2014-05-30 | 国立大学法人東北大学 | エリスロポエチン発現増強剤 |
WO2017072816A1 (ja) | 2015-10-29 | 2017-05-04 | 国立大学法人東北大学 | 臓器線維化疾患の治療剤 |
JP6872195B2 (ja) | 2015-10-29 | 2021-05-19 | 国立大学法人東北大学 | コラーゲン産生抑制剤 |
WO2017072984A1 (ja) | 2015-10-29 | 2017-05-04 | 株式会社東北テクノアーチ | 筋萎縮性疾患治療剤 |
JP6857346B2 (ja) | 2016-11-11 | 2021-04-14 | 国立大学法人東北大学 | ライソゾーム病の予防又は改善剤 |
-
2019
- 2019-06-04 JP JP2020523109A patent/JP7373804B2/ja active Active
- 2019-06-04 US US15/734,579 patent/US20220106270A1/en active Pending
- 2019-06-04 WO PCT/JP2019/022082 patent/WO2019235455A1/ja active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2019235455A1 (ja) | 2019-12-12 |
JP7373804B2 (ja) | 2023-11-06 |
US20220106270A1 (en) | 2022-04-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1272468B1 (fr) | Derives d'indolin-2-one et leur utilisation en tant que ligands des recepteurs de l'ocytocine | |
CN1080117C (zh) | 用具有rxr类视色素受体激动剂活性的化合物治疗患肿瘤的哺乳动物 | |
CN1053886C (zh) | 萘基化合物、中间体、制法和应用 | |
CN1067082C (zh) | 用于治疗和预防内毒素血症的取代的脂多糖 | |
CN104803891B (zh) | 胰高血糖素拮抗剂 | |
TW200404060A (en) | Fused heterocyclic compounds | |
CN101068798A (zh) | 1-环丙基甲基-4-[2-(3,3,5,5-四甲基环己基)苯基]哌嗪的盐及结晶 | |
KR20140101399A (ko) | 불화 에스트로겐 수용체 조절제 및 이의 용도 | |
JP6372817B2 (ja) | 臓器線維化抑制剤 | |
JP2017095499A (ja) | Ccr2の縮合シクロペンチル拮抗薬 | |
WO1999042100A1 (fr) | Inhibiteurs de la mort cellulaire | |
US7276531B2 (en) | G-lactam derivatives as prostaglandin agonists | |
JP6857346B2 (ja) | ライソゾーム病の予防又は改善剤 | |
JP7373804B2 (ja) | 精子の受精機能増強剤 | |
CN111499591A (zh) | RORγ调节剂 | |
JP6977990B2 (ja) | 軟骨基質分解酵素の産生抑制剤 | |
CN1017426B (zh) | 烷基褪黑激素的制备方法 | |
CN1409715A (zh) | 环戊烯酮衍生物 | |
US20070232605A1 (en) | Inhibitors of soluble adenylate cyclase | |
CN1610679A (zh) | 制备因子xa抑制剂的有效方法 | |
EP0215319B1 (en) | Gem-dihalo-1,8-diamino-4-aza-octanes | |
WO2017072816A1 (ja) | 臓器線維化疾患の治療剤 | |
CN1330630A (zh) | 作为细胞凋亡诱导化合物的7-芳基-6(z)庚三烯酸维生素a酰胺及其作为抗癌药剂的应用 | |
EP0402232A1 (fr) | Dérivés de pyridobenzoindole, leur préparation et les compositions qui les contiennent | |
CN1426404A (zh) | Ⅰ型钠-氢交换剂(nhe-1)抑制剂 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20201202 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20220425 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20230605 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20230731 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20231011 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20231016 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7373804 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |