JPWO2019192489A5 - - Google Patents
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- JPWO2019192489A5 JPWO2019192489A5 JP2019566740A JP2019566740A JPWO2019192489A5 JP WO2019192489 A5 JPWO2019192489 A5 JP WO2019192489A5 JP 2019566740 A JP2019566740 A JP 2019566740A JP 2019566740 A JP2019566740 A JP 2019566740A JP WO2019192489 A5 JPWO2019192489 A5 JP WO2019192489A5
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- JP
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- Prior art keywords
- primer
- aspects
- adapter
- pool
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 description 82
- 108091033319 polynucleotide Proteins 0.000 description 56
- 102000040430 polynucleotide Human genes 0.000 description 56
- 239000002157 polynucleotide Substances 0.000 description 56
- 238000006243 chemical reaction Methods 0.000 description 36
- 239000002773 nucleotide Substances 0.000 description 33
- 125000003729 nucleotide group Chemical group 0.000 description 33
- 230000003321 amplification Effects 0.000 description 21
- 238000003199 nucleic acid amplification method Methods 0.000 description 21
- 238000012163 sequencing technique Methods 0.000 description 7
- 238000006116 polymerization reaction Methods 0.000 description 6
- 108020004414 DNA Proteins 0.000 description 4
- 102000004357 Transferases Human genes 0.000 description 4
- 108090000992 Transferases Proteins 0.000 description 4
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 239000011541 reaction mixture Substances 0.000 description 4
- 230000000379 polymerizing effect Effects 0.000 description 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 230000030609 dephosphorylation Effects 0.000 description 2
- 238000006209 dephosphorylation reaction Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024063835A JP2024096852A (ja) | 2018-04-03 | 2024-04-11 | 核酸ライブラリー作製のための組成物及び方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2018/081748 WO2019191900A1 (en) | 2018-04-03 | 2018-04-03 | Compositions and methods for preparing nucleic acid libraries |
| CNPCT/CN2018/081748 | 2018-04-03 | ||
| PCT/CN2019/081059 WO2019192489A1 (en) | 2018-04-03 | 2019-04-02 | Compositions and methods for preparing nucleic acid libraries |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024063835A Division JP2024096852A (ja) | 2018-04-03 | 2024-04-11 | 核酸ライブラリー作製のための組成物及び方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021517556A JP2021517556A (ja) | 2021-07-26 |
| JP2021517556A5 JP2021517556A5 (https=) | 2022-04-11 |
| JPWO2019192489A5 true JPWO2019192489A5 (https=) | 2022-04-11 |
Family
ID=68099745
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019566740A Pending JP2021517556A (ja) | 2018-04-03 | 2019-04-02 | 核酸ライブラリー作製のための組成物及び方法 |
| JP2024063835A Pending JP2024096852A (ja) | 2018-04-03 | 2024-04-11 | 核酸ライブラリー作製のための組成物及び方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024063835A Pending JP2024096852A (ja) | 2018-04-03 | 2024-04-11 | 核酸ライブラリー作製のための組成物及び方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20210040475A1 (https=) |
| EP (1) | EP3740604A4 (https=) |
| JP (2) | JP2021517556A (https=) |
| CN (2) | CN110892097A (https=) |
| AU (1) | AU2019248276A1 (https=) |
| BR (1) | BR112020020207A2 (https=) |
| CA (1) | CA3095837A1 (https=) |
| SG (1) | SG11202009774XA (https=) |
| WO (2) | WO2019191900A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111455469B (zh) * | 2020-04-07 | 2023-08-18 | 深圳易倍科华生物科技有限公司 | 一种单链快速建库方法及建库仪器 |
| IL302823A (en) * | 2020-11-10 | 2023-07-01 | Us Health | Single-cell profiling of chromatin occupancy and rna sequencing |
| CN112538657B (zh) * | 2020-12-25 | 2021-08-17 | 北京吉因加医学检验实验室有限公司 | 一种脑脊液基因测序建库、检测方法及其应用 |
| CN113564226A (zh) * | 2021-07-26 | 2021-10-29 | 深圳泰莱生物科技有限公司 | 一种捕获cfDNA5mC片段的检测方法 |
| CN115896027A (zh) * | 2022-04-07 | 2023-04-04 | 广州燃石医学检验所有限公司 | 一种生物组合物、其制备方法及应用 |
| CN114736951B (zh) * | 2022-04-20 | 2024-11-12 | 深圳大学 | 一种小分子rna的高通量测序文库构建方法 |
| CN116287124A (zh) * | 2023-05-24 | 2023-06-23 | 中国农业科学院农业基因组研究所 | 单链接头预连接方法、高通量测序文库的建库方法及试剂盒 |
| WO2025072689A1 (en) * | 2023-09-28 | 2025-04-03 | The Regents Of The University Of California | Methods and compositions for producing sequencing libraries |
| WO2025147442A1 (en) * | 2024-01-02 | 2025-07-10 | Grail, Inc. | Rapid bisulfite conversion and recovery of nucleic acid molecules |
Family Cites Families (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| US5705365A (en) | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5710029A (en) | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| ATE374833T1 (de) | 1998-11-09 | 2007-10-15 | Eiken Chemical | Verfahren zur synthese von nukleinsäuren |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| WO2002072773A2 (en) | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
| JP4632788B2 (ja) | 2002-09-20 | 2011-02-16 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 核酸のヘリカーゼ依存性増幅 |
| WO2005095654A1 (en) | 2004-03-25 | 2005-10-13 | Biohelix Corporation | Helicase-dependent amplification of circular nucleic acids |
| WO2010037001A2 (en) | 2008-09-26 | 2010-04-01 | Immune Disease Institute, Inc. | Selective oxidation of 5-methylcytosine by tet-family proteins |
| CA2811185C (en) | 2010-09-21 | 2020-09-22 | Population Genetics Technologies Ltd. | Increasing confidence of allele calls with molecular counting |
| EP4372084A3 (en) * | 2012-01-26 | 2024-08-14 | Tecan Genomics, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
| US9528107B2 (en) * | 2012-01-31 | 2016-12-27 | Pacific Biosciences Of California, Inc. | Compositions and methods for selection of nucleic acids |
| SG11201405669XA (en) * | 2012-03-13 | 2014-10-30 | Swift Biosciences Inc | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
| US9862995B2 (en) | 2012-03-13 | 2018-01-09 | Abhijit Ajit Patel | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
| US9092401B2 (en) | 2012-10-31 | 2015-07-28 | Counsyl, Inc. | System and methods for detecting genetic variation |
| ES2669512T3 (es) | 2012-11-30 | 2018-05-28 | Cambridge Epigenetix Limited | Agente oxidante para nucleótidos modificados |
| AU2014214682B2 (en) * | 2013-02-08 | 2018-07-26 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| WO2014143157A1 (en) * | 2013-03-13 | 2014-09-18 | The Board Institute, Inc. | Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets |
| CN104946737B (zh) | 2013-12-11 | 2019-02-22 | 安可济控股有限公司 | 用于检测罕见序列变体的组合物和方法 |
| KR102321956B1 (ko) * | 2014-01-31 | 2021-11-08 | 스위프트 바이오사이언시스 인코포레이티드 | Dna 기질을 처리하는 개선 방법 |
| GB201403216D0 (en) * | 2014-02-24 | 2014-04-09 | Cambridge Epigenetix Ltd | Nucleic acid sample preparation |
| US10208338B2 (en) * | 2014-03-03 | 2019-02-19 | Swift Biosciences, Inc. | Enhanced adaptor ligation |
| CN104264231B (zh) * | 2014-09-30 | 2017-04-19 | 天津华大基因科技有限公司 | 构建测序文库的方法及其应用 |
| WO2016077602A1 (en) | 2014-11-12 | 2016-05-19 | Ibis Biosciences, Inc. | Next generation sequencing methods |
| EP4488686A3 (en) * | 2016-04-14 | 2025-04-30 | Guardant Health, Inc. | Methods for early detection of cancer |
| WO2017218777A1 (en) * | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| CN106192021B (zh) * | 2016-08-02 | 2017-04-26 | 中国海洋大学 | 一种串联rad标签测序文库的构建方法 |
| CN106497920A (zh) * | 2016-11-21 | 2017-03-15 | 深圳华大基因研究院 | 一种用于非小细胞肺癌基因突变检测的文库构建方法及试剂盒 |
| WO2019023924A1 (en) * | 2017-08-01 | 2019-02-07 | Helitec Limited | METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES |
-
2018
- 2018-04-03 WO PCT/CN2018/081748 patent/WO2019191900A1/en not_active Ceased
-
2019
- 2019-04-02 SG SG11202009774XA patent/SG11202009774XA/en unknown
- 2019-04-02 BR BR112020020207-0A patent/BR112020020207A2/pt unknown
- 2019-04-02 EP EP19769980.4A patent/EP3740604A4/en active Pending
- 2019-04-02 JP JP2019566740A patent/JP2021517556A/ja active Pending
- 2019-04-02 US US17/044,723 patent/US20210040475A1/en not_active Abandoned
- 2019-04-02 CN CN201980002533.4A patent/CN110892097A/zh active Pending
- 2019-04-02 CN CN202110396910.6A patent/CN113106145B/zh active Active
- 2019-04-02 AU AU2019248276A patent/AU2019248276A1/en not_active Abandoned
- 2019-04-02 CA CA3095837A patent/CA3095837A1/en active Pending
- 2019-04-02 WO PCT/CN2019/081059 patent/WO2019192489A1/en not_active Ceased
-
2021
- 2021-04-07 US US17/225,082 patent/US12460202B2/en active Active
-
2024
- 2024-04-11 JP JP2024063835A patent/JP2024096852A/ja active Pending
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