SG11202009774XA - Compositions and methods for preparing nucleic acid libraries - Google Patents

Compositions and methods for preparing nucleic acid libraries

Info

Publication number
SG11202009774XA
SG11202009774XA SG11202009774XA SG11202009774XA SG11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA
Authority
SG
Singapore
Prior art keywords
compositions
methods
nucleic acid
acid libraries
preparing nucleic
Prior art date
Application number
SG11202009774XA
Other languages
English (en)
Inventor
Zhihong Zhang
Tao Zheng
Bingsi Li
Wanglong Deng
Yusheng Han
Original Assignee
Guangzhou Burning Rock Dx Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Burning Rock Dx Co Ltd filed Critical Guangzhou Burning Rock Dx Co Ltd
Publication of SG11202009774XA publication Critical patent/SG11202009774XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11202009774XA 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries SG11202009774XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/CN2018/081748 WO2019191900A1 (en) 2018-04-03 2018-04-03 Compositions and methods for preparing nucleic acid libraries
PCT/CN2019/081059 WO2019192489A1 (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

Publications (1)

Publication Number Publication Date
SG11202009774XA true SG11202009774XA (en) 2020-10-29

Family

ID=68099745

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202009774XA SG11202009774XA (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

Country Status (9)

Country Link
US (2) US20210040475A1 (https=)
EP (1) EP3740604A4 (https=)
JP (2) JP2021517556A (https=)
CN (2) CN110892097A (https=)
AU (1) AU2019248276A1 (https=)
BR (1) BR112020020207A2 (https=)
CA (1) CA3095837A1 (https=)
SG (1) SG11202009774XA (https=)
WO (2) WO2019191900A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111455469B (zh) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
IL302823A (en) * 2020-11-10 2023-07-01 Us Health Single-cell profiling of chromatin occupancy and rna sequencing
CN112538657B (zh) * 2020-12-25 2021-08-17 北京吉因加医学检验实验室有限公司 一种脑脊液基因测序建库、检测方法及其应用
CN113564226A (zh) * 2021-07-26 2021-10-29 深圳泰莱生物科技有限公司 一种捕获cfDNA5mC片段的检测方法
CN115896027A (zh) * 2022-04-07 2023-04-04 广州燃石医学检验所有限公司 一种生物组合物、其制备方法及应用
CN114736951B (zh) * 2022-04-20 2024-11-12 深圳大学 一种小分子rna的高通量测序文库构建方法
CN116287124A (zh) * 2023-05-24 2023-06-23 中国农业科学院农业基因组研究所 单链接头预连接方法、高通量测序文库的建库方法及试剂盒
WO2025072689A1 (en) * 2023-09-28 2025-04-03 The Regents Of The University Of California Methods and compositions for producing sequencing libraries
WO2025147442A1 (en) * 2024-01-02 2025-07-10 Grail, Inc. Rapid bisulfite conversion and recovery of nucleic acid molecules

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
ATE374833T1 (de) 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2002072773A2 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
JP4632788B2 (ja) 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
WO2005095654A1 (en) 2004-03-25 2005-10-13 Biohelix Corporation Helicase-dependent amplification of circular nucleic acids
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
CA2811185C (en) 2010-09-21 2020-09-22 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
EP4372084A3 (en) * 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US9528107B2 (en) * 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
SG11201405669XA (en) * 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
ES2669512T3 (es) 2012-11-30 2018-05-28 Cambridge Epigenetix Limited Agente oxidante para nucleótidos modificados
AU2014214682B2 (en) * 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
WO2014143157A1 (en) * 2013-03-13 2014-09-18 The Board Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets
CN104946737B (zh) 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
KR102321956B1 (ko) * 2014-01-31 2021-11-08 스위프트 바이오사이언시스 인코포레이티드 Dna 기질을 처리하는 개선 방법
GB201403216D0 (en) * 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
US10208338B2 (en) * 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
CN104264231B (zh) * 2014-09-30 2017-04-19 天津华大基因科技有限公司 构建测序文库的方法及其应用
WO2016077602A1 (en) 2014-11-12 2016-05-19 Ibis Biosciences, Inc. Next generation sequencing methods
EP4488686A3 (en) * 2016-04-14 2025-04-30 Guardant Health, Inc. Methods for early detection of cancer
WO2017218777A1 (en) * 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
CN106192021B (zh) * 2016-08-02 2017-04-26 中国海洋大学 一种串联rad标签测序文库的构建方法
CN106497920A (zh) * 2016-11-21 2017-03-15 深圳华大基因研究院 一种用于非小细胞肺癌基因突变检测的文库构建方法及试剂盒
WO2019023924A1 (en) * 2017-08-01 2019-02-07 Helitec Limited METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES

Also Published As

Publication number Publication date
CN110892097A (zh) 2020-03-17
BR112020020207A2 (pt) 2021-01-19
WO2019191900A1 (en) 2019-10-10
EP3740604A1 (en) 2020-11-25
US12460202B2 (en) 2025-11-04
AU2019248276A1 (en) 2020-10-22
CA3095837A1 (en) 2019-10-10
JP2024096852A (ja) 2024-07-17
JP2021517556A (ja) 2021-07-26
CN113106145B (zh) 2024-10-11
EP3740604A4 (en) 2021-12-29
WO2019192489A1 (en) 2019-10-10
CN113106145A (zh) 2021-07-13
US20210040475A1 (en) 2021-02-11
US20210254051A1 (en) 2021-08-19

Similar Documents

Publication Publication Date Title
IL286945A (en) Methods and preparations for nucleic acid analysis
SG11202009774XA (en) Compositions and methods for preparing nucleic acid libraries
IL273817A (en) Preparations and methods for editing rna
IL277837A (en) Gene editing compositions and methods
SG11202106666UA (en) Methods for rna analysis
SG10202108763UA (en) Methods and compositions for nucleic acid analysis
IL267034A (en) Methods and preparations for administering nucleic acid and/or protein cargo
IL253196A0 (en) Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using them
GB2565461B (en) Methods and compositions for the single tube preparation of sequencing libraries using Cas9
GB201905651D0 (en) Nucleic acid constructs and methods for their manufacture
SG11201610357UA (en) Methods and compositions for preparing sequencing libraries
GB201903914D0 (en) De novo synthesized nucleic acid libraries
IL271232A (en) Compounds and methods for editing the genome
SG10202111266VA (en) Compositions and methods for library construction and sequence analysis
WO2017107898A9 (en) Compositions and methods for gene editing
IL260122A (en) Methods and compositions for assembly of nucleic acids
SG11202108563QA (en) Methods for nucleic acid analysis
IL286587A (en) Preparations of d-methyrosine and methods for their preparation
EP3476938A4 (en) PROCESS AND KIT FOR SYNTHESIS OF NUCLEIC ACID UNDER CONSTANT TEMPERATURE CONDITIONS
AU2019405977B2 (en) Compositions and methods for detecting Plasmodium species nucleic acid
GB202104190D0 (en) Compositions and methods for detecting group b streptococcu nucleic acid
PL3393589T3 (pl) Sposoby i kompozycje do łatwego usuwania powłok z paznokci
GB201803240D0 (en) Methods and compositions for enriching nucleic acids
HK40033053A (en) Compositions and methods for preparing nucleic acid libraries
HK40018044A (en) Methods and compositions for preparing nucleic acid libraries