SG11202009774XA - Compositions and methods for preparing nucleic acid libraries - Google Patents

Compositions and methods for preparing nucleic acid libraries

Info

Publication number
SG11202009774XA
SG11202009774XA SG11202009774XA SG11202009774XA SG11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA SG 11202009774X A SG11202009774X A SG 11202009774XA
Authority
SG
Singapore
Prior art keywords
compositions
methods
nucleic acid
acid libraries
preparing nucleic
Prior art date
Application number
SG11202009774XA
Inventor
Zhihong Zhang
Tao Zheng
Bingsi Li
Wanglong Deng
Yusheng Han
Original Assignee
Guangzhou Burning Rock Dx Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Burning Rock Dx Co Ltd filed Critical Guangzhou Burning Rock Dx Co Ltd
Publication of SG11202009774XA publication Critical patent/SG11202009774XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11202009774XA 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries SG11202009774XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/CN2018/081748 WO2019191900A1 (en) 2018-04-03 2018-04-03 Compositions and methods for preparing nucleic acid libraries
PCT/CN2019/081059 WO2019192489A1 (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

Publications (1)

Publication Number Publication Date
SG11202009774XA true SG11202009774XA (en) 2020-10-29

Family

ID=68099745

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202009774XA SG11202009774XA (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

Country Status (9)

Country Link
US (2) US20210040475A1 (en)
EP (1) EP3740604A4 (en)
JP (2) JP2021517556A (en)
CN (2) CN113106145B (en)
AU (1) AU2019248276A1 (en)
BR (1) BR112020020207A2 (en)
CA (1) CA3095837A1 (en)
SG (1) SG11202009774XA (en)
WO (2) WO2019191900A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111455469B (en) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 Single-chain rapid library construction method and library construction instrument
EP4244381A4 (en) * 2020-11-10 2024-07-31 Us Health Single-cell profiling of chromatin occupancy and rna sequencing
CN112538657B (en) * 2020-12-25 2021-08-17 北京吉因加医学检验实验室有限公司 Cerebrospinal fluid gene sequencing library building and detecting method and application thereof
CN113564226A (en) * 2021-07-26 2021-10-29 深圳泰莱生物科技有限公司 Detection method for capturing cfDNA5mC fragment
WO2023193456A1 (en) * 2022-04-07 2023-10-12 广州燃石医学检验所有限公司 Biological composition, method for preparing same, and use thereof
CN114736951A (en) * 2022-04-20 2022-07-12 深圳大学 High-throughput sequencing library construction method for small-molecule RNA
CN116287124A (en) * 2023-05-24 2023-06-23 中国农业科学院农业基因组研究所 Single-stranded joint pre-connection method, library construction method of high-throughput sequencing library and kit

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4372084A3 (en) * 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CN104395480B (en) * 2012-03-13 2018-01-30 斯威夫特生物科学公司 For the method and composition of size-controlled homopolymeric tailing to be carried out to substrate polynucleotide by nucleic acid polymerase
WO2014071070A1 (en) * 2012-11-01 2014-05-08 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
CN108753766A (en) * 2013-02-08 2018-11-06 10X基因组学有限公司 Polynucleotides bar code generating at
WO2014143157A1 (en) * 2013-03-13 2014-09-18 The Board Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets
ES2908644T3 (en) * 2014-01-31 2022-05-03 Swift Biosciences Inc Improved procedures for processing DNA substrates
GB201403216D0 (en) * 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
EP3114231B1 (en) * 2014-03-03 2019-01-02 Swift Biosciences, Inc. Enhanced adaptor ligation
CN104264231B (en) * 2014-09-30 2017-04-19 天津华大基因科技有限公司 Method for constructing sequencing library and application of sequencing library
EP3443066B1 (en) * 2016-04-14 2024-10-02 Guardant Health, Inc. Methods for early detection of cancer
CN109642252A (en) * 2016-06-17 2019-04-16 加州理工学院 Nucleic acid reaction and correlation technique and composition
CN106192021B (en) * 2016-08-02 2017-04-26 中国海洋大学 Method for constructing series connection RAD [restriction-site-associated DNA (deoxyribonucleic acid)] tag sequencing libraries
CN106497920A (en) * 2016-11-21 2017-03-15 深圳华大基因研究院 A kind of library constructing method and test kit for nonsmall-cell lung cancer detection in Gene Mutation
JP6998404B2 (en) * 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 Method for enriching and determining the target nucleotide sequence

Also Published As

Publication number Publication date
EP3740604A4 (en) 2021-12-29
AU2019248276A1 (en) 2020-10-22
JP2021517556A (en) 2021-07-26
WO2019192489A1 (en) 2019-10-10
CN110892097A (en) 2020-03-17
US20210040475A1 (en) 2021-02-11
JP2024096852A (en) 2024-07-17
CA3095837A1 (en) 2019-10-10
CN113106145A (en) 2021-07-13
EP3740604A1 (en) 2020-11-25
US20210254051A1 (en) 2021-08-19
CN113106145B (en) 2024-10-11
BR112020020207A2 (en) 2021-01-19
WO2019191900A1 (en) 2019-10-10

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