BR112020020207A2 - Método para preparação de uma biblioteca de polinucleotídeos, composição, polinucleotídeo e kit para preparação de uma biblioteca de polinucleotídeos - Google Patents

Método para preparação de uma biblioteca de polinucleotídeos, composição, polinucleotídeo e kit para preparação de uma biblioteca de polinucleotídeos Download PDF

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Publication number
BR112020020207A2
BR112020020207A2 BR112020020207-0A BR112020020207A BR112020020207A2 BR 112020020207 A2 BR112020020207 A2 BR 112020020207A2 BR 112020020207 A BR112020020207 A BR 112020020207A BR 112020020207 A2 BR112020020207 A2 BR 112020020207A2
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BR
Brazil
Prior art keywords
primer
adapter
sequence
pool
tail
Prior art date
Application number
BR112020020207-0A
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English (en)
Portuguese (pt)
Inventor
Zhihong Zhang
Tao Zheng
Bingsi LI
Wanglong DENG
Yusheng HAN
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Guangzhou Burning Rock Dx Co., Ltd.
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Application filed by Guangzhou Burning Rock Dx Co., Ltd. filed Critical Guangzhou Burning Rock Dx Co., Ltd.
Publication of BR112020020207A2 publication Critical patent/BR112020020207A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112020020207-0A 2018-04-03 2019-04-02 Método para preparação de uma biblioteca de polinucleotídeos, composição, polinucleotídeo e kit para preparação de uma biblioteca de polinucleotídeos BR112020020207A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/CN2018/081748 WO2019191900A1 (en) 2018-04-03 2018-04-03 Compositions and methods for preparing nucleic acid libraries
WOPCT/CN2018/081748 2018-04-03
PCT/CN2019/081059 WO2019192489A1 (en) 2018-04-03 2019-04-02 Compositions and methods for preparing nucleic acid libraries

Publications (1)

Publication Number Publication Date
BR112020020207A2 true BR112020020207A2 (pt) 2021-01-19

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BR112020020207-0A BR112020020207A2 (pt) 2018-04-03 2019-04-02 Método para preparação de uma biblioteca de polinucleotídeos, composição, polinucleotídeo e kit para preparação de uma biblioteca de polinucleotídeos

Country Status (9)

Country Link
US (2) US20210040475A1 (https=)
EP (1) EP3740604A4 (https=)
JP (2) JP2021517556A (https=)
CN (2) CN110892097A (https=)
AU (1) AU2019248276A1 (https=)
BR (1) BR112020020207A2 (https=)
CA (1) CA3095837A1 (https=)
SG (1) SG11202009774XA (https=)
WO (2) WO2019191900A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111455469B (zh) * 2020-04-07 2023-08-18 深圳易倍科华生物科技有限公司 一种单链快速建库方法及建库仪器
IL302823A (en) * 2020-11-10 2023-07-01 Us Health Single-cell profiling of chromatin occupancy and rna sequencing
CN112538657B (zh) * 2020-12-25 2021-08-17 北京吉因加医学检验实验室有限公司 一种脑脊液基因测序建库、检测方法及其应用
CN113564226A (zh) * 2021-07-26 2021-10-29 深圳泰莱生物科技有限公司 一种捕获cfDNA5mC片段的检测方法
CN115896027A (zh) * 2022-04-07 2023-04-04 广州燃石医学检验所有限公司 一种生物组合物、其制备方法及应用
CN114736951B (zh) * 2022-04-20 2024-11-12 深圳大学 一种小分子rna的高通量测序文库构建方法
CN116287124A (zh) * 2023-05-24 2023-06-23 中国农业科学院农业基因组研究所 单链接头预连接方法、高通量测序文库的建库方法及试剂盒
WO2025072689A1 (en) * 2023-09-28 2025-04-03 The Regents Of The University Of California Methods and compositions for producing sequencing libraries
WO2025147442A1 (en) * 2024-01-02 2025-07-10 Grail, Inc. Rapid bisulfite conversion and recovery of nucleic acid molecules

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
ATE374833T1 (de) 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2002072773A2 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
JP4632788B2 (ja) 2002-09-20 2011-02-16 ニュー・イングランド・バイオラブズ・インコーポレイティッド 核酸のヘリカーゼ依存性増幅
WO2005095654A1 (en) 2004-03-25 2005-10-13 Biohelix Corporation Helicase-dependent amplification of circular nucleic acids
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
CA2811185C (en) 2010-09-21 2020-09-22 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
EP4372084A3 (en) * 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US9528107B2 (en) * 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
SG11201405669XA (en) * 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
ES2669512T3 (es) 2012-11-30 2018-05-28 Cambridge Epigenetix Limited Agente oxidante para nucleótidos modificados
AU2014214682B2 (en) * 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
WO2014143157A1 (en) * 2013-03-13 2014-09-18 The Board Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets
CN104946737B (zh) 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
KR102321956B1 (ko) * 2014-01-31 2021-11-08 스위프트 바이오사이언시스 인코포레이티드 Dna 기질을 처리하는 개선 방법
GB201403216D0 (en) * 2014-02-24 2014-04-09 Cambridge Epigenetix Ltd Nucleic acid sample preparation
US10208338B2 (en) * 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
CN104264231B (zh) * 2014-09-30 2017-04-19 天津华大基因科技有限公司 构建测序文库的方法及其应用
WO2016077602A1 (en) 2014-11-12 2016-05-19 Ibis Biosciences, Inc. Next generation sequencing methods
EP4488686A3 (en) * 2016-04-14 2025-04-30 Guardant Health, Inc. Methods for early detection of cancer
WO2017218777A1 (en) * 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
CN106192021B (zh) * 2016-08-02 2017-04-26 中国海洋大学 一种串联rad标签测序文库的构建方法
CN106497920A (zh) * 2016-11-21 2017-03-15 深圳华大基因研究院 一种用于非小细胞肺癌基因突变检测的文库构建方法及试剂盒
WO2019023924A1 (en) * 2017-08-01 2019-02-07 Helitec Limited METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES

Also Published As

Publication number Publication date
CN110892097A (zh) 2020-03-17
WO2019191900A1 (en) 2019-10-10
EP3740604A1 (en) 2020-11-25
US12460202B2 (en) 2025-11-04
AU2019248276A1 (en) 2020-10-22
CA3095837A1 (en) 2019-10-10
JP2024096852A (ja) 2024-07-17
JP2021517556A (ja) 2021-07-26
CN113106145B (zh) 2024-10-11
SG11202009774XA (en) 2020-10-29
EP3740604A4 (en) 2021-12-29
WO2019192489A1 (en) 2019-10-10
CN113106145A (zh) 2021-07-13
US20210040475A1 (en) 2021-02-11
US20210254051A1 (en) 2021-08-19

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