JPWO2012011563A1 - 高密度リポタンパク質3中のコレステロールの定量方法 - Google Patents
高密度リポタンパク質3中のコレステロールの定量方法 Download PDFInfo
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- JPWO2012011563A1 JPWO2012011563A1 JP2012525439A JP2012525439A JPWO2012011563A1 JP WO2012011563 A1 JPWO2012011563 A1 JP WO2012011563A1 JP 2012525439 A JP2012525439 A JP 2012525439A JP 2012525439 A JP2012525439 A JP 2012525439A JP WO2012011563 A1 JPWO2012011563 A1 JP WO2012011563A1
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- cholesterol
- hdl3
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
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- Organic Chemistry (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
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- Endocrinology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
以下の試薬組成の試薬Aと試薬Bを調製し、試薬Aに各種界面活性剤を0.1%(w/v)または1.0%(W/V)濃度添加した試薬をそれぞれ調製した。測定直前に各種界面活性剤入りの下記試薬Aと試薬Bを1:3の割合で混合し、HDL2分画とHDL3分画のコレステロールを反応させ、主波長700nm、副波長600nmでの終吸光度を測定し比較した。
BES緩衝液 (pH7.0) 100mmol/L
HDAOS 0.7mmol/L
カタラーゼ 600U/L
コレステロールオキシダーゼ 1.4U/mL
コレステロールエステラーゼ 0.8U/mL
BES緩衝液 (pH6.6) 100mmol/L
アジ化ナトリウム 0.1%
4−アミノアンチピリン 4.0mmol/L
ペルオキシダーゼ 2.4U/mL
超遠心を用いてCM〜LDL分画、HDL2分画、HDL3分画を画分し、下記試薬Jを反応させ、さらに、下記試薬Kを添加し、測定を行なった。測定は、血清2μLに試薬Eを150μL添加し、5分加温反応の後、試薬Kを添加しさらに5分加温反応させ、主波長700nm、副波長600nmの吸光度を測定した。
BES緩衝液 (pH7.0) 100mmol/L
HDAOS 0.7mmol/L
プルロニックP103 0.03w/v%
カタラーゼ 600U/L
コレステロールオキシダーゼ 1.4U/mL
コレステロールエステラーゼ 0.8U/mL
ホスフォリパーゼD 5.0U/mL
BES緩衝液 (pH6.6) 100mmol/L
アジ化ナトリウム 0.1%
エマルゲン120 1.0%
4−アミノアンチピリン 4.0mmol/L
ペルオキシダーゼ 2.4U/mL
Claims (3)
- 被検試料に高密度リポタンパク質3と特異的に反応する界面活性剤を反応させ、コレステロールを定量することを含む、高密度リポタンパク質3中のコレステロールの定量方法。
- 前記界面活性剤が、ポリオキシエチレンジスチレン化フェニルエーテル、ポリオキシエチレンラウリルエーテル及びP−イソオクチルポリオキシエチレンフェノールホルムアルデヒドポリマーから成る群より選ばれる少なくとも1種の非イオン界面活性剤;ラウリルジメチル−アミノ酢酸ベタイン及びポリオキシエチレンラウリルエーテルから成る群より選ばれる少なくとも1種の両性界面活性剤;及び/又は脂肪酸族リン酸エステルから成る群より選ばれる少なくとも1種の陽イオン界面活性剤である請求項1記載の方法。
- 前記界面活性剤が、ポリオキシエチレンラウリルエーテルである請求項2記載の方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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JP2012525439A JP5653434B2 (ja) | 2010-07-23 | 2011-07-22 | 高密度リポタンパク質3中のコレステロールの定量方法 |
Applications Claiming Priority (4)
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JP2010166395 | 2010-07-23 | ||
JP2010166395 | 2010-07-23 | ||
JP2012525439A JP5653434B2 (ja) | 2010-07-23 | 2011-07-22 | 高密度リポタンパク質3中のコレステロールの定量方法 |
PCT/JP2011/066691 WO2012011563A1 (ja) | 2010-07-23 | 2011-07-22 | 高密度リポタンパク質3中のコレステロールの定量方法 |
Publications (2)
Publication Number | Publication Date |
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JPWO2012011563A1 true JPWO2012011563A1 (ja) | 2013-09-09 |
JP5653434B2 JP5653434B2 (ja) | 2015-01-14 |
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Country | Link |
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US (1) | US9151768B2 (ja) |
EP (1) | EP2607901A4 (ja) |
JP (1) | JP5653434B2 (ja) |
KR (1) | KR101891984B1 (ja) |
CN (1) | CN103124906B (ja) |
AU (1) | AU2011280496B2 (ja) |
CA (1) | CA2809111C (ja) |
WO (1) | WO2012011563A1 (ja) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104245952B (zh) * | 2012-03-30 | 2017-08-29 | 积水医疗株式会社 | 血液样品中的物质的测定法 |
CN107267594B (zh) * | 2012-08-31 | 2022-05-17 | 日立化成诊断系统株式会社 | 高密度脂蛋白中的胆固醇的测定方法 |
JP6182008B2 (ja) * | 2013-07-24 | 2017-08-16 | デンカ生研株式会社 | 高密度リポタンパク質3中のコレステロールの定量方法及び定量試薬 |
JP2017060522A (ja) * | 2016-12-20 | 2017-03-30 | デンカ生研株式会社 | 高密度リポ蛋白(hdl)中のコレステロールの定量方法 |
JP7251722B2 (ja) * | 2018-09-27 | 2023-04-04 | 国立大学法人九州大学 | 糖尿病に関する情報を取得する方法及びその利用 |
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US9024A (en) * | 1852-06-15 | Motion of the lay in looms | ||
JP2799835B2 (ja) * | 1995-01-31 | 1998-09-21 | 第一化学薬品株式会社 | コレステロールの定量方法 |
AU735659B2 (en) | 1997-04-14 | 2001-07-12 | Denka Seiken Co., Ltd. | Method for quantifying cholesterol present in low density lipoprotein |
JP4708531B2 (ja) * | 2000-06-07 | 2011-06-22 | シスメックス株式会社 | Hdl亜画分中のコレステロールの測定法 |
CN1379235A (zh) * | 2002-05-10 | 2002-11-13 | 肖洪武 | 高密度脂蛋白胆固醇测定方法及试剂 |
US20050037504A1 (en) * | 2003-07-17 | 2005-02-17 | Dimagno Theodore John | One-step assay for high-density lipoprotein cholesterol |
CN100564539C (zh) * | 2004-12-31 | 2009-12-02 | 浙江伊利康生物技术有限公司 | 高密度脂蛋白中胆固醇的测定试剂及制备方法 |
GB0609493D0 (en) * | 2006-05-12 | 2006-06-21 | Oxford Biosensors Ltd | Cholesterol sensor |
EP2027471A1 (en) * | 2006-05-12 | 2009-02-25 | Oxford Biosensors Limited | Hdl cholesterol sensor using selective surfactant |
JP5450080B2 (ja) * | 2007-10-10 | 2014-03-26 | デンカ生研株式会社 | small,denseLDLコレステロールの定量方法およびキット |
JP5500777B2 (ja) * | 2008-03-06 | 2014-05-21 | デンカ生研株式会社 | Hdl3−cの測定方法及びhdl3−c測定用試薬 |
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- 2011-07-22 EP EP20110809733 patent/EP2607901A4/en not_active Withdrawn
- 2011-07-22 CA CA2809111A patent/CA2809111C/en active Active
- 2011-07-22 WO PCT/JP2011/066691 patent/WO2012011563A1/ja active Application Filing
- 2011-07-22 CN CN201180036065.6A patent/CN103124906B/zh active Active
- 2011-07-22 US US13/811,569 patent/US9151768B2/en active Active
- 2011-07-22 AU AU2011280496A patent/AU2011280496B2/en active Active
- 2011-07-22 KR KR1020137003552A patent/KR101891984B1/ko active IP Right Grant
- 2011-07-22 JP JP2012525439A patent/JP5653434B2/ja active Active
Also Published As
Publication number | Publication date |
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US20130164769A1 (en) | 2013-06-27 |
AU2011280496B2 (en) | 2015-08-20 |
WO2012011563A1 (ja) | 2012-01-26 |
KR20130043185A (ko) | 2013-04-29 |
CA2809111A1 (en) | 2012-01-26 |
EP2607901A1 (en) | 2013-06-26 |
KR101891984B1 (ko) | 2018-08-27 |
CN103124906B (zh) | 2016-08-17 |
CA2809111C (en) | 2018-05-01 |
AU2011280496A1 (en) | 2013-03-14 |
US9151768B2 (en) | 2015-10-06 |
JP5653434B2 (ja) | 2015-01-14 |
EP2607901A4 (en) | 2014-01-15 |
CN103124906A (zh) | 2013-05-29 |
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