JPWO2010101209A1 - 表面増強ラマン散乱活性測定基板 - Google Patents
表面増強ラマン散乱活性測定基板 Download PDFInfo
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- JPWO2010101209A1 JPWO2010101209A1 JP2011502794A JP2011502794A JPWO2010101209A1 JP WO2010101209 A1 JPWO2010101209 A1 JP WO2010101209A1 JP 2011502794 A JP2011502794 A JP 2011502794A JP 2011502794 A JP2011502794 A JP 2011502794A JP WO2010101209 A1 JPWO2010101209 A1 JP WO2010101209A1
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- metal
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- enhanced raman
- aggregation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
Abstract
Description
上述したように、金属基板は凝集対象の金属ナノ粒子又はクラスタとの関係で電極電位を考慮して選択されるが、銀ナノ粒子またはクラスタを凝集させるには銅板、真鍮板又はリン青銅板等の銅合金板が選択されるのが好ましい。この銀ナノクラスタ分散液を金属基板上で凝集させると、金属基板上にSERS活性を有するクラスタが配位子とともに結晶を形成するものと思われ(図5)、ホットサイトの作成に適する凝集領域となる。
Claims (11)
- SERS活性を有する金属の粒径100nm以下のナノ粒子(クラスタを含む)100〜5000ppmを含む分散液をその金属の電極電位より卑なる(高い)電極電位を有する金属基板上で凝集させ、所望の凝集状態で凝集停止させてなり、凝集領域が表面増強ラマン散乱(SERS)測定のためのホットサイトを形成していることを特徴とする表面増強ラマン散乱光測定用基板。
- 前記分散液中のSERS活性を有する金属が銀であって、分散液中に銀イオンと金属錯体を形成する配位子を供給する配位化合物を含む請求項1記載の表面増強ラマン散乱光測定用基板。
- 前記配位化合物が銀とのアンミン錯体を形成するアミノ基を有する請求項2記載の表面増強ラマン散乱光測定用基板。
- 前記配位化合物がアミノ基とカルボキシル基とを有する両性界面活性剤である請求項3記載の表面増強ラマン散乱光測定用基板。
- 金属基板が銅板又は銅合金である請求項2記載の表面増強ラマン散乱光測定用基板。
- 表面増強ラマン活性機能を有する金属の100nm以下の粒子径のナノ粒子(クラスタを含む)を含む分散液を表面増強ラマン活性機能を有する金属の金属電極電位より卑なる(高い)電極電位を有する金属表面を有する基板上で凝集開始後適時凝集停止させて最適凝集領域を提供する工程と、該金属凝集域に被検出試料を付着させる工程と、被検出試料の非乾燥状態で金属ナノ粒子表面に吸着させた被検出試料に所定のレーザ光を照射し被検出試料から発生するラマン散乱光を測定する工程を含むことを特徴とする表面増強ラマン散乱光測定方法。
- 粒径5〜100nmの金または銀ナノ粒子又はクラスタ100〜5000ppmを含む分散液中に少なくともアミノ基とカルボニル基を有する両性電解質を分散させ、該両性電解質を含む分散液を金属基板上に滴下し、金属基板とナノ金属との電極電位差で凝集を開始させ、最適凝集状態で凝集を停止させた後両性電解質をその等電点以上または以下のpH溶液で処理してプラスまたはマイナス帯電させることにより、被検出タンパク試料を吸着させ、金または銀ナノ粒子にレーザ光を照射し、吸着した被検出タンパク試料から発生するラマン散乱光を測定することを特徴とする表面増強ラマン散乱光測定方法。
- 請求項6又は7に記載の測定方法に用いられる分散液であって、表面増強ラマン活性機能を有する金属イオンの分散液中に金属イオンに配位子を供給する配位化合物を添加して金属錯体クラスタを形成してなる表面増強ラマン活性機能を有する金属ナノ粒子分散液。
- 前記配位化合物がアミノ基とカルボキシル基を含む両性界面活性剤である請求項8記載のタンパク検出用分散液。
- 抗原抗体反応の抗体成分を単独で又は前記両性界面活性剤とともに添加した請求項8記載の抗原抗体反応用分散液。
- 前記両性界面活性剤がアミノ酸系界面活性剤を含むタンパク系界面活性剤であり、抗体成分又は抗原成分を金属ナノ粒子又はクラスタ間に誘導する機能を有する請求項10に記載の分散液。
Priority Applications (1)
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JP2011502794A JP5466226B2 (ja) | 2009-03-04 | 2010-03-04 | 表面増強ラマン散乱活性測定基板 |
Applications Claiming Priority (6)
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JP2009051105 | 2009-03-04 | ||
JP2009051105 | 2009-03-04 | ||
JP2009171354 | 2009-07-22 | ||
JP2009171354 | 2009-07-22 | ||
JP2011502794A JP5466226B2 (ja) | 2009-03-04 | 2010-03-04 | 表面増強ラマン散乱活性測定基板 |
PCT/JP2010/053509 WO2010101209A1 (ja) | 2009-03-04 | 2010-03-04 | 表面増強ラマン散乱活性測定基板 |
Publications (2)
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JPWO2010101209A1 true JPWO2010101209A1 (ja) | 2012-09-10 |
JP5466226B2 JP5466226B2 (ja) | 2014-04-09 |
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JP2011502794A Expired - Fee Related JP5466226B2 (ja) | 2009-03-04 | 2010-03-04 | 表面増強ラマン散乱活性測定基板 |
Country Status (6)
Country | Link |
---|---|
US (1) | US9658163B2 (ja) |
EP (1) | EP2405257A4 (ja) |
JP (1) | JP5466226B2 (ja) |
KR (1) | KR20120022754A (ja) |
CN (1) | CN102428359B (ja) |
WO (1) | WO2010101209A1 (ja) |
Families Citing this family (21)
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US9139907B2 (en) * | 2010-09-06 | 2015-09-22 | Mytech Co., Ltd | Method for producing metal complex quantum crystals |
JP5801587B2 (ja) * | 2011-03-31 | 2015-10-28 | 富士フイルム株式会社 | 光電場増強デバイスの製造方法 |
JP6196159B2 (ja) * | 2011-10-31 | 2017-09-13 | 有限会社マイテック | 金属錯体量子結晶及びそれを用いる生化学物質の表面増強ラマン散乱(sers)分析法 |
KR101538218B1 (ko) * | 2012-08-24 | 2015-07-22 | 한양대학교 에리카산학협력단 | 표면―증강 라만 산란에 기초한 고속 및 고감도 미량 분석용 유무기 나노섬유 복합체 기판 및 이의 제조방법 |
CN102818800A (zh) * | 2012-09-07 | 2012-12-12 | 天津大学 | 一种基于芯片级试纸的人血尿蛋白检测方法 |
CN103901247B (zh) * | 2012-12-28 | 2016-08-31 | 清华大学 | 电势差测量方法 |
WO2014178935A1 (en) * | 2013-04-29 | 2014-11-06 | Imra America, Inc. | Size controlled suspension of precious metal nanoparticles |
JP6294614B2 (ja) * | 2013-05-08 | 2018-03-14 | 有限会社マイテック | 癌関連物質の定量方法 |
CN103604796A (zh) * | 2013-11-29 | 2014-02-26 | 苏州大学 | 一种硅基表面增强拉曼散射(sers)基底的制备方法 |
ES2905092T3 (es) * | 2014-05-08 | 2022-04-07 | Mytech Co Ltd | Chip plasmónico y procedimientos de diagnóstico de enfermedades cancerígenas empleando una imagen fluorescente y espectroscopia Raman respectivamente |
CN103968946B (zh) * | 2014-05-19 | 2016-01-13 | 中国人民解放军第二军医大学 | 一种表面增强拉曼二维相关光谱的采集方法 |
KR101765387B1 (ko) * | 2015-06-24 | 2017-08-23 | 서강대학교산학협력단 | 금속 코아 간 초미세 보이드를 가지는 나노 갭 구조체 및 이를 이용한 분자 검출 장치 및 방법, 선택적 에칭을 통한 상기 나노 갭 구조체의 제조 방법 |
CN106814059B (zh) * | 2017-01-16 | 2019-06-14 | 北京芯创睿胜科技有限公司 | Sers活性液滴、制备方法及分子检测方法 |
CN107262168B (zh) * | 2017-06-12 | 2019-04-30 | 重庆大学 | 一种pdms自吸进样的微流控sers芯片及其制备方法 |
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JP7307917B2 (ja) * | 2019-04-26 | 2023-07-13 | 国立大学法人群馬大学 | 生体高分子の濃縮化方法、結晶化方法およびナノ構造基板 |
CN113092443B (zh) * | 2021-04-12 | 2022-09-20 | 华北电力大学 | 一种粘性Cu-Au贵金属复合基底、制备方法及其应用 |
CN113275583B (zh) * | 2021-05-11 | 2022-07-01 | 安徽大学 | 面向农药残留的sers基底制备方法及检测方法 |
US20230035300A1 (en) * | 2021-07-28 | 2023-02-02 | National Cheng Kung University | Method for detecting viruses |
CN114184592B (zh) * | 2021-11-24 | 2023-12-05 | 厦门大学 | 一种基于负电性sers基底的负电性分子sers检测方法 |
WO2023123069A1 (zh) * | 2021-12-29 | 2023-07-06 | 陆一平 | 一种可调整材料表面对电磁波反应的方法和其组合构造 |
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WO2009117646A2 (en) * | 2008-03-20 | 2009-09-24 | Drexel University | Method for the formation of sers substrates |
US9139907B2 (en) * | 2010-09-06 | 2015-09-22 | Mytech Co., Ltd | Method for producing metal complex quantum crystals |
-
2010
- 2010-03-04 KR KR1020117023287A patent/KR20120022754A/ko not_active Application Discontinuation
- 2010-03-04 US US13/255,071 patent/US9658163B2/en not_active Expired - Fee Related
- 2010-03-04 WO PCT/JP2010/053509 patent/WO2010101209A1/ja active Application Filing
- 2010-03-04 CN CN201080019304.2A patent/CN102428359B/zh not_active Expired - Fee Related
- 2010-03-04 EP EP10748802.5A patent/EP2405257A4/en not_active Withdrawn
- 2010-03-04 JP JP2011502794A patent/JP5466226B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
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CN102428359A (zh) | 2012-04-25 |
KR20120022754A (ko) | 2012-03-12 |
EP2405257A4 (en) | 2017-08-02 |
JP5466226B2 (ja) | 2014-04-09 |
US20120115245A1 (en) | 2012-05-10 |
WO2010101209A1 (ja) | 2010-09-10 |
CN102428359B (zh) | 2014-12-31 |
US9658163B2 (en) | 2017-05-23 |
EP2405257A1 (en) | 2012-01-11 |
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