JPS63501571A - Ｄ−ルシフェリン誘導体、および生化学物質定量に当っての酵素標識リガンド検出へのその利用および利用法 - Google Patents

Ｄ−ルシフェリン誘導体、および生化学物質定量に当っての酵素標識リガンド検出へのその利用および利用法

Info

Publication number
JPS63501571A
JPS63501571A JP61505774A JP50577486A JPS63501571A JP S63501571 A JPS63501571 A JP S63501571A JP 61505774 A JP61505774 A JP 61505774A JP 50577486 A JP50577486 A JP 50577486A JP S63501571 A JPS63501571 A JP S63501571A
Authority
JP
Japan
Prior art keywords
group
luciferin
enzyme
residue
general formula
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP61505774A
Other languages
English (en)
Japanese (ja)
Inventor
ガイガ−，ラインハルト
ミスカ，ウエルナー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JPS63501571A publication Critical patent/JPS63501571A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62—Benzothiazoles
    • C07D277/64—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65583—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0821—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/18—Kallidins; Bradykinins; Related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Luminescent Compositions (AREA)
  • Peptides Or Proteins (AREA)
JP61505774A 1985-10-24 1986-10-24 Ｄ−ルシフェリン誘導体、および生化学物質定量に当っての酵素標識リガンド検出へのその利用および利用法 Pending JPS63501571A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19853537877 DE3537877A1 (de) 1985-10-24 1985-10-24 Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate
DE3537877.8 1985-10-24

Publications (1)

Publication Number Publication Date
JPS63501571A true JPS63501571A (ja) 1988-06-16

Family

ID=6284380

Family Applications (1)

Application Number Title Priority Date Filing Date
JP61505774A Pending JPS63501571A (ja) 1985-10-24 1986-10-24 Ｄ−ルシフェリン誘導体、および生化学物質定量に当っての酵素標識リガンド検出へのその利用および利用法

Country Status (5)

Country Link
US (1) US5098828A (enExample)
EP (1) EP0243429B1 (enExample)
JP (1) JPS63501571A (enExample)
DE (2) DE3537877A1 (enExample)
WO (1) WO1987002667A1 (enExample)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998028569A1 (en) * 1996-12-20 1998-07-02 Kikkoman Corporation Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member
JP2007526766A (ja) * 2004-01-22 2007-09-20 プロメガ　コーポレイション ルミネセンス発生及びルミネセンス非発生多重アッセイ
JP2008545746A (ja) * 2005-05-31 2008-12-18 プロメガ コーポレイション 分子または状態を検出するための発光性化合物および蛍光性化合物、ならびに方法
US8071328B2 (en) 2002-02-01 2011-12-06 Promega Corporation Bioluminescent protease assay
US8227205B2 (en) 2007-04-13 2012-07-24 Promega Corporation Luminescent live and dead cell assay
US8288559B2 (en) 2008-08-18 2012-10-16 Promega Corporation Luminogenic compounds and methods to detect cytochrome P450 3A enzymes
JP2012533654A (ja) * 2009-07-16 2012-12-27 ベックマン コールター， インコーポレイテッド 新規な蛍光色素およびその使用
US8476036B2 (en) 2004-01-22 2013-07-02 Promega Corporation Nonluminogenic assay for living cells
US8765969B2 (en) 2002-09-20 2014-07-01 Promega Corporation Luminescence-based methods and probes for measuring cytochrome P450 activity
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3700908A1 (de) * 1987-01-14 1988-07-28 Geiger Reinhard Aminoluciferine, verfahren zu deren herstellung und deren verwendung bei der bestimmung von enzymaktivitaeten
WO1990000618A1 (en) * 1988-07-08 1990-01-25 Jbl Scientific, Inc. Preparation and use of fluorescent benzothiazole derivatives
FR2644163B2 (fr) * 1988-07-13 1994-09-30 Bioprobe Systems Sa Procede de marquage d'une sonde nucleique et trousse de reactifs pour la mise en oeuvre de ce procede
DE3916729A1 (de) * 1989-05-23 1990-12-06 Behringwerke Ag Fluorogene verbindungen und ihre verwendung
WO1991005872A1 (en) * 1989-10-17 1991-05-02 British Technology Group Ltd Enhanced chemiluminescent assay
US5374534A (en) * 1990-07-19 1994-12-20 Charm Sciences, Inc. Method of preparing D-luciferin derivatives
US5283180A (en) * 1990-07-19 1994-02-01 Charm Sciences, Inc. Bioluminescence method for the determination of pesticides
US5972630A (en) * 1991-08-19 1999-10-26 Dade Behring Marburg Gmbh Homogeneous immunoassays using enzyme inhibitors
JPH08506897A (ja) * 1993-02-12 1996-07-23 シーライト・サイエンシーズ・インコーポレーテツド 発光蛋白質複合体の調製法およびその使用法
US5648218A (en) * 1993-02-12 1997-07-15 Sealite Sciences, Inc. Preparation of photoprotein conjugates and methods of use thereof
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
US6458547B1 (en) 1996-12-12 2002-10-01 Prolume, Ltd. Apparatus and method for detecting and identifying infectious agents
US6140051A (en) * 1997-07-21 2000-10-31 Promega Biosciences, Inc. Fluorescent dibenzazole derivatives and methods related thereto
EP1064360B1 (en) 1998-03-27 2008-03-05 Prolume, Ltd. Luciferases, gfp fluorescent proteins, their nucleic acids and the use thereof in diagnostics
EP1925320A3 (en) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics
WO2000004007A1 (en) * 1998-07-17 2000-01-27 Promega Biosciences, Inc. Fluorescent dibenzazole derivatives and methods related thereto
DE19918636A1 (de) * 1999-04-23 2000-10-26 Intermedical S A H Verfahren zum lumineszenten Nachweis von Antigenen
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US20020073441A1 (en) 2000-12-13 2002-06-13 Ross Brian D. Compositions and methods for detecting proteolytic activity
CA2448824A1 (en) * 2001-06-01 2002-12-12 F. Hoffmann-La Roche Ag Novel compounds for chemiluminescense procedures
US20040248225A1 (en) * 2001-06-01 2004-12-09 Dieter Heindl Luciferin hydrazides
US7268229B2 (en) * 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
JP2009506772A (ja) * 2005-09-01 2009-02-19 プロメガ コーポレイション 細胞ベースの発光及び非発光プロテアソームアッセイ
EP1991702A4 (en) * 2006-02-17 2009-04-22 Univ Leland Stanford Junior SEQUENCING REPORTERGEN LUMINESCENCE (SRL) PROCESS AND COMPOSITION FOR ITS IMPLEMENTATION
US7893272B2 (en) * 2007-05-15 2011-02-22 Cellex, Inc. Reagents and kits for detection of influenza virus and the like
US9029092B2 (en) 2009-02-27 2015-05-12 Beckman Coulter, Inc. Solution phase homogeneous assays
US20110213124A1 (en) * 2010-02-26 2011-09-01 Gryshuk Amy L Methods and systems for synthesis of a d-aminoluciferin precursor and related compounds
US9370588B2 (en) 2013-01-04 2016-06-21 University Of Massachusetts Luciferin amides
PL3529616T3 (pl) 2016-10-20 2024-03-18 President And Fellows Of Harvard College Testy in vitro i komórkowe do pomiaru aktywności neurotoksyn botulinowych
US11136615B2 (en) 2017-08-04 2021-10-05 Promega Corporation Compositions and methods for stabilizing benzothiazole luciferin analogs
US20230375558A1 (en) 2020-09-22 2023-11-23 3Helix, Inc. Methods for Using Collagen Hybridizing Peptides to Determine Collagen Content
WO2023028688A1 (en) 2021-09-01 2023-03-09 Nyoka Design Corp. Reusable photoluminescent apparatus, methods, and systems

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE7811630L (sv) * 1977-11-17 1979-05-18 Welsh Nat School Med Metod for uppteckt eller mengdbestemning av substanser med anvendning av merkningsteknik
US4380580A (en) * 1978-04-10 1983-04-19 Miles Laboratories, Inc. Heterogenous chemiluminescent specific binding assay
DE2929115A1 (de) * 1979-07-18 1981-02-12 Boehringer Mannheim Gmbh Verfahren zur herstellung von luciferin
WO1983003104A1 (en) * 1982-03-03 1983-09-15 Carter, Timothy, Joseph, Nicholas Enhanced luminescent and luminometric assay
US4665022A (en) * 1984-02-17 1987-05-12 The Regents Of The University Of California Bioluminescent assay reagent and method
US4649108A (en) * 1984-07-26 1987-03-10 Genzyme Corporation Alpha amylase assay

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6521304B1 (en) 1996-12-20 2003-02-18 Kikkoman Corporation Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member
WO1998028569A1 (en) * 1996-12-20 1998-07-02 Kikkoman Corporation Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member
US8071328B2 (en) 2002-02-01 2011-12-06 Promega Corporation Bioluminescent protease assay
US8765969B2 (en) 2002-09-20 2014-07-01 Promega Corporation Luminescence-based methods and probes for measuring cytochrome P450 activity
US10408819B2 (en) 2002-09-20 2019-09-10 Promega Corporation Luminescence-based methods and probes for measuring cytochrome P450 activity
US9574223B2 (en) 2002-09-20 2017-02-21 Promega Corporation Luminescence-based methods and probes for measuring cytochrome P450 activity
JP2011188857A (ja) * 2004-01-22 2011-09-29 Promega Corp ルミネセンス発生及びルミネセンス非発生多重アッセイ
US8476036B2 (en) 2004-01-22 2013-07-02 Promega Corporation Nonluminogenic assay for living cells
US8715950B2 (en) 2004-01-22 2014-05-06 Promega Corporation Kits for luminogenic and nonluminogenic multiplex assays
JP2007526766A (ja) * 2004-01-22 2007-09-20 プロメガ　コーポレイション ルミネセンス発生及びルミネセンス非発生多重アッセイ
US8476450B2 (en) 2005-05-31 2013-07-02 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
JP2008545746A (ja) * 2005-05-31 2008-12-18 プロメガ コーポレイション 分子または状態を検出するための発光性化合物および蛍光性化合物、ならびに方法
US10077244B2 (en) 2005-05-31 2018-09-18 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US10745365B2 (en) 2005-05-31 2020-08-18 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US8227205B2 (en) 2007-04-13 2012-07-24 Promega Corporation Luminescent live and dead cell assay
US8288559B2 (en) 2008-08-18 2012-10-16 Promega Corporation Luminogenic compounds and methods to detect cytochrome P450 3A enzymes
JP2012533654A (ja) * 2009-07-16 2012-12-27 ベックマン コールター， インコーポレイテッド 新規な蛍光色素およびその使用
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements

Also Published As

Publication number Publication date
EP0243429A1 (de) 1987-11-04
DE3537877C2 (enExample) 1989-08-24
DE3677206D1 (de) 1991-02-28
DE3537877A1 (de) 1987-04-30
WO1987002667A1 (fr) 1987-05-07
US5098828A (en) 1992-03-24
EP0243429B1 (de) 1991-01-23

Similar Documents

Publication Publication Date Title
JPS63501571A (ja) Ｄ−ルシフェリン誘導体、および生化学物質定量に当っての酵素標識リガンド検出へのその利用および利用法
JP2870704B2 (ja) ホモシステインアッセイ
US5198537A (en) Digoxigenin derivatives and use thereof
Diamandis et al. The biotin-(strept) avidin system: principles and applications in biotechnology
US4383031A (en) Homogeneous chemiluminescent specific binding assay
Kanaoka Organic fluorescence reagents in the study of enzymes and proteins
EP1008600B1 (en) Compositions and methods for generating red chemiluminescence
JPH0137694B2 (enExample)
JPS58757A (ja) 同位体を使用しない免疫検定を実施する方法、ラベルされた分析物とかかる検定に使用するキット
US6139781A (en) Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes
US3880934A (en) Nitrophenyloxy-butanediols
US4791055A (en) Homogenous specific binding assay reagent system and labeled conjugates
JP2000502989A (ja) 改善された化学発光性１，２−ジオキセタン類
EP0234799A2 (en) Method of protein detection or isolation
AU582341B2 (en) Assay for immobilized reporter groups
JPH04507194A (ja) 化学発光組成物、化学発光法、及び分析的検定におけるその使用
CA2295304A1 (en) Fluorescent dibenzazole derivatives and methods related thereto
US20070020681A1 (en) Hydrolytic substrates for an analyte-dependent enzyme activation system
CA1304291C (en) Catalyst transformation by a modified reaction intermediate as a means for enzyme immunoassay
US4123614A (en) Novel assay reagents
JPH10104230A (ja) 核酸等の検出方法，並びに標識物質及び検出物質
Josel et al. Luminescence Systems
JPH0431763A (ja) 修飾されたキレート化剤を用いる蛍光分析方法
JPH04507102A (ja) 新しいペプチド基質，調整法及びプロティンｃ定量における用途
CZ62092A3 (cs) Prostriedok na označovanie bielkovín, nukleových kyselin a iných chemických zlúčenín