JPS63290808A - Cosmetic - Google Patents

Abstract

PURPOSE:To provide a safe cosmetic for skin or oral cavity, containing a water- soluble acid salt of chitosan as an active component, having high deodorization and antibacterial properties and exhibiting excellent bio-compatibility because of the absence of stimulation, toxicity, allergic activity, etc. CONSTITUTION:The objective antibacterial deodorant cosmetic contains >=1wt.% of a water-soluble acid salt of chitosan (e.g. hydrochloride, formate, acetate or L-aspartate). The chitosan salt can be produced by depolymerizing chitosan to a molecular weight of <=100,000, dispersing in water and adding an acid in an amount to give an aqueous chitosan salt solution having a pH of 3-6 under agitation. The above chitosan salt is suitable as a face lotion or cream for pimple, deodorant, gargle, wiping agent, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] This invention relates to a cosmetic that exhibits deodorizing and antibacterial effects.

[Conventional technology]

BACKGROUND ART Some cosmetics for the skin or the mouth contain antibacterial substances and are intended to have deodorizing and antibacterial effects. For example, lotions and creams for acne, deodorants, gargles, cleansing agents, etc.
Various antibacterial substances are blended depending on the form of the product. Furthermore, these antibacterial substances are often blended into cosmetics other than those mentioned above in order to preserve the cosmetics themselves.

Examples of antibacterial substances include Oxidol (hydrogen peroxide) and Gyakusei Stone! (cation activator), chlorthymol, salicylic acid, zalol (phenyl salicylate), carbolic acid (phenol), nitrofurazone, hinokitiol, hexachlorophene, boric acid, sodium borate, etc. are commonly used.

[Problem that the invention seeks to solve]

However, in addition to deodorizing and antibacterial properties, the above-mentioned antibacterial substances generally have side effects such as irritation, toxicity, and allergy to the human body to a greater or lesser extent.
When manufacturing cosmetics, there are restrictions on the type, amount, etc. of antibacterial substances that can be added depending on the use, purpose, etc. At the same time, care must be taken in actual use.

In view of the above circumstances, this invention provides cosmetics containing safe antibacterial substances that are highly biocompatible and highly deodorizing and antibacterial, and are not irritating, toxic, or allergic. The purpose is to provide fees.

[Means for solving problems]

In view of the above problems, the inventors focused on antibacterial substances that exist in nature, and investigated the relationship between the deodorizing properties, antibacterial properties, and biocompatibility of various compounds. As a result, it was found that chitosan obtained from chitin has excellent deodorizing properties, antibacterial properties, and biocompatibility. However, such chitosan is a solid that is insoluble in water in its normal state, and it has been difficult to apply it as it is to the cosmetics in various forms such as solutions and emulsions. Therefore, □As a result of further consideration,
completed this invention.

That is, the gist of the present invention is a cosmetic composition characterized by containing chitosan as an active ingredient in the form of a water-soluble acid salt.

[For production]

The cosmetics of this invention will be explained in detail below.

The water-soluble acid salt of chitosan (hereinafter referred to as "chitosan salt") used in this invention is the carapace of crustaceans such as shrimp and crabs,
Chitosan is obtained by acetylating chitin obtained from mushrooms, bacterial cell walls, etc., and is obtained by reacting it with an organic or inorganic acid.

Chitosan generally has an average molecular weight of 200,000 or more and has low solubility in acids, so it tends to be unable to react sufficiently with acids in its original state. Therefore, in the reaction with an acid, it is desirable to lower the molecular weight of chitosan to 100,000 or less in advance and then react with the acid.

Of course, chitosan may be used as it is without reducing its molecular weight.

Methods for reducing the molecular weight of chitosan include chemical treatment using oxidizing agents, papain, and cellulase.

Examples include enzyme treatment methods using enzymes such as acid protease.

Acids that react with chitosan as mentioned above include inorganic acids,
Any organic acid may be used. Examples of inorganic acids include hydrochloric acid, and examples of organic acids include formic acid, lactic acid, acetic acid, citric acid, glycolic acid, malonic acid, ascorbic acid, adipic acid, oxalic acid, propionic acid, tartaric acid, succinic acid, and L.
-Glutamic acid, L-aspartic acid, etc.

In addition, when sulfuric acid is used as the acid, the obtained chitosan salt is not water-soluble but water-insoluble, and sufficient antibacterial action cannot be obtained. Therefore, the chitosan salt blended into the cosmetic of the present invention needs to be a water-soluble acid salt that is a reactant with each of the above acids.

In order to react these chitosan and acids, it is sufficient to add acid to the chitosan powder, but if only these substances are used, the reaction may occur rapidly or side reactions other than the intended reaction may occur. Therefore, these reactions are usually carried out in the presence of water.

Further, this water is also required to make the chitosan salt produced by the reaction into an aqueous solution of a predetermined concentration.

As a method for such a reaction involving water, there is a method such as dispersing chitosan powder in water and adding an acid while stirring it, but other methods may also be used. In addition, in reacting chitosan with an acid as described above, the degrading enzyme for reducing the molecular weight of chitosan can also be added to the reaction system, thereby reducing the molecular weight of chitosan and melting it at the same time. .

The amount of acid to be blended is not particularly limited in the present invention, but it is preferable to blend the acid in an amount greater than or equal to the amount necessary to neutralize the free amino groups of chitosan, and the pH of the resulting aqueous solution of chitosan salt is within the range of 3 to 6. It is more preferable to blend the amount.

The type of cosmetic is not particularly limited as long as the above-mentioned chitosan salt is blended, and the present invention includes the above-mentioned acne lotion, cream, deodorant, gargle, cleaning agent, etc. , for skin.

It can be applied to all kinds of cosmetics, such as two-in-one use for shaving.

Examples of these cosmetics include the following, but it goes without saying that other cosmetics than those shown here may also be used.

■ Animal and vegetable oils, animal and vegetable waxes, mineral waxes, hydrocarbons,
Cleansing creams and cleansing creams that are oil-based, including oily components such as fatty acids, or emulsifying agents, such as nonionic surfactants, cationic surfactants, 9 amphoteric surfactants, etc., added to the above oily components. lotion,
Mucilage lotion, emulsion (emulsifying lotion), two-layer lotion,
Creams, foundations, deodorant creams, jelly shampoos, cream shampoos, shaving creams, etc.

■ Aqueous solution or gel containing water-soluble sugars, water-soluble polymer compounds, etc., or alcohol-based lotion, deodorant lotion, liquid shampoo, shaving lotion, and cleaning lotion. , mouthwash, etc.

■ Powdered, solid or paste soaps, howder foundation, baby powder, antiperspirant powder, powdered shampoo, toothpaste, etc.

Depending on the form of the cosmetic, the chitosan salt may be blended in the form of an aqueous solution, or it may be blended after removing water and turning it into powder. It may be.

Among the above-mentioned forms, since the presence of water is undesirable in oil-based and powdered forms, powdered chitosan salt is blended. Note that depending on the manufacturing method, chitosan salt can be blended as an aqueous solution, even if it is oil-based or powdered.

In other forms, chitosan salt may be blended as an aqueous solution, but powdered chitosan salt may also be used. In this case, there is an advantage that the concentration of chitosan salt can be higher than that in an aqueous solution. As a method for pulverizing chitosan salt in this manner, ordinary drying methods such as spray drying and freeze drying can be used.

Although the amount of chitosan salt added is not particularly limited in the present invention, it is preferable that the amount of chitosan salt added to the cosmetic is 1% by weight or more. This is because if the amount of chitosan salt added is less than 1% by weight, sufficient antibacterial and deodorizing effects may not be obtained.

In order to investigate the deodorizing and antibacterial effects of these chitosan salts, the following tests were conducted.

Estimated molecular weight 15,000 estimated from the intrinsic viscosity when 1% by weight of target chitosan is dissolved in 1% by weight acetic acid aqueous solution
A tributicase soy broth medium (culture solution) containing 0.025 to 0.25% by weight of chitosan lactate was prepared. Next, the following 5 types of bacterial strains that cause odor and spoilage were cultured in Trypticase soy broth medium for 2 days at 37°C until the number of bacteria per 1 d was 100 to 1000. Trypticase soy broth medium containing chitosan lactate was inoculated as described above.

(Used strain) Staphylococcus aureus Bacillus
llus 5ubtilis Bacillus segaterium Lactob
acillus plantaluta Lactoba
cillus sporogenes Each of the above images and the culture medium are as follows.

・5taphylococcus aureus:
A typical example of pyogenes is pyogenes, also called staphylococcus.

・Bacillus 5ubtilis: Bacillus subtilis,
It is an aerobic or facultative anaerobic bacterium that is airborne.

・Bacillus megaterium: A type of heterotrophic bacterium, it is a saprophytic bacterium, a parasitic bacterium, a pathogenic bacterium, or
There are symbiotic bacteria.

・Lactobacillus plantalum
: Converts carbohydrates into lactic acid with Lactobacillus. It often lives in the digestive tract and stomach of animals.

・Lactobacillus sporogenes
: Bacteria involved in rancidity, which gives off a foul odor and causes rot.

- Trypticase soy broth medium: Formed by dissolving the following ingredient combination in purified water 11.

Polypeptone (casein peptone) 17g Polypeptone (soybean peptone) 3g Sodium chloride 5g Phosphoric acid 2
Potassium 2.5g Glucose
2.5g Soy bean casein digest agar medium (described later) 2. Add the following ingredients to purified water 11.
It is formed by melting into a substance, and is used for bacterial testing of pharmaceuticals and cosmetics.

Polypeptone (casein peptone) 15g Polypeptone (soybean peptone) 5g Sodium chloride
5g agar
A trypticase soy broth medium inoculated with 15 g of the above strain was cultured at 30°C, and the growth status was determined after 3 and 7 days. The determination is made by smearing and culturing the above medium on the soybean casein digest agar medium,
Judgment was made based on the degree of aggregation of the bacterial strain used and the aggregation of culture medium proteins due to chitosan. In addition, trypticase soy broth medium without the addition of chitosan was similarly cultured, and the results were used as a control.

The results are shown in Table 1. The judgment symbols in Table 1 are as follows.

From the results shown in Table 1, it was found that the products to which chitosan salt was added were able to inhibit the growth of these bacterial strains relatively compared to the products to which chitosan salt was not added. It was also found that the products to which chitosan salt was added did not generate any odor and had high odor resistance.

〔Example〕

Next, examples of the present invention will be described together with comparative examples.

(Examples 1 to 6. Comparative Example 1) Among the components shown below, four components from glyceryl monostearate to cetanol were mixed and melted at 80 ° C.
This was added to purified water heated to 80°C, emulsified using a homogenizer, and cooled to room temperature to obtain a milky lotion. A chitosan lactate aqueous solution (pH 5.0,
5% by weight aqueous solution) was added to prepare a sample. The chitosan lactate used has an estimated molecular weight of 1500 from the intrinsic viscosity.
It was 0.

Ingredient combination> After heating the obtained sample to 80℃ and sterilizing it, we cultured it in advance in trypticase soy broth medium at 37℃ for 2 days. For each strain, the number of bacteria per 11 is 1000 ~
The total number of vaccinations was 10,000.

(Used strain) Staphylococcus aureus Bacillus
llus 5ubtilis Escherichia coli Of the above, the first two are as described above, and Escherichia coli is En te
It belongs to the robac tariaceae family, is a Durham-negative, non-sporulating bacillus that parasitizes animals, and is a representative bacterium of the enterobacteria group. The presence of these bacteria serves as an indicator of the degree of contamination, indicating the risk of pathogenic contamination. The presence of this type of bacteria degrades skin secretions and causes odor, so it was tested here as a representative of Durham-negative contaminant bacteria.

These samples were cultured at 30°C, and the growth status was determined after 7 and 30 days. Judgment was made by culturing the above-mentioned medium solution on a soy bean casein digest agar medium and based on the degree of flocculation of the strain used and the flocculation of the medium protein due to chitosan. In addition, the deterioration of the emulsion was determined by the change in its appearance, and the effect was determined by combining both.

The results are shown in Table 2. The judgment symbols in Table 2 are as follows.

From the results in Table 2, Examples 1 to 6 of the cosmetics of this invention
Both have higher antibacterial effects than Comparative Example 1,
It was found that it has both a sterilizing effect on the skin and its own antiseptic effect.

(Examples 7 to 10) Deodorant lotions having the following ingredient formulations were prepared and used as sample solutions.

Component Blend> The following tests were conducted using the sample solutions prepared in Examples 7 to 10 above.

(Test) A piece of pharmacopoeia gauze with a size of approximately 20 cm square was folded into four. On one side, the sample solutions of Examples 7 to 10 were placed, and on the other side, a control solution prepared by excluding only the chitosan salt from the above ingredient combination was placed. , each of which was sprayed to contain approximately ice was prepared. The gauze sprayed with the sample solution was applied under the right armpit of 10 subjects, and the gauze sprayed with the control solution was applied below the left knee of 10 subjects with paper tape, and the gauze was transferred to the gauze after about 12 hours. Participants themselves evaluated the differences in body odor. The results are shown in Table 3.

Furthermore, after the test, when the armpits that had been in contact with the gauze were observed, no allergic symptoms due to irritation were observed at any chitosan concentration.

From the results in Table 3, Examples 7 to 1 of the cosmetics of this invention
According to O, including those with slight differences in effectiveness,
It was found that a clear deodorizing effect can be obtained.

〔Effect of the invention〕

As described above, the cosmetic of the present invention contains chitosan as an active ingredient in the form of a water-soluble acid salt, so it has high deodorizing and antibacterial properties, and is free from irritation, toxicity, and
Since it has no allergic properties, it is safe and has excellent biocompatibility.

Claims (2)

[Claims] (1) A cosmetic product characterized by containing chitosan as an active ingredient in the form of a water-soluble acid salt. (2) The cosmetic according to claim 1, wherein the amount of the water-soluble acid salt of chitosan added is 10% by weight or more.