JP2004307484A - Antiseptic bactericide, and cosmetic, pharmaceutical and foodstuff blended with the same - Google Patents
Antiseptic bactericide, and cosmetic, pharmaceutical and foodstuff blended with the same Download PDFInfo
- Publication number
- JP2004307484A JP2004307484A JP2004082232A JP2004082232A JP2004307484A JP 2004307484 A JP2004307484 A JP 2004307484A JP 2004082232 A JP2004082232 A JP 2004082232A JP 2004082232 A JP2004082232 A JP 2004082232A JP 2004307484 A JP2004307484 A JP 2004307484A
- Authority
- JP
- Japan
- Prior art keywords
- acid
- antiseptic
- alkanediol
- chelating agent
- blended
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 239000002738 chelating agent Substances 0.000 claims abstract description 29
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- 235000013305 food Nutrition 0.000 claims description 17
- AEIJTFQOBWATKX-UHFFFAOYSA-N octane-1,2-diol Chemical compound CCCCCCC(O)CO AEIJTFQOBWATKX-UHFFFAOYSA-N 0.000 claims description 17
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- Cosmetics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Non-Alcoholic Beverages (AREA)
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Abstract
Description
本発明は防腐殺菌剤並びに該防腐殺菌剤を配合した化粧料、医薬品及び食品に関する。本発明の目的は、炭素数5〜10の1,2−アルカンジオールとキレート剤とを含有することにより、1,2−アルカンジオールが本来有する抗菌活性を増強することができる防腐殺菌剤並びに該防腐殺菌剤を配合した化粧品、医薬品及び食品を提供することにある。 The present invention relates to an antiseptic disinfectant and cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant. An object of the present invention is to contain a 1,2-alkanediol having 5 to 10 carbon atoms and a chelating agent to thereby enhance the antibacterial activity inherent to 1,2-alkanediol, An object of the present invention is to provide cosmetics, medicines, and foods containing an antiseptic and a disinfectant.
化粧品(医薬部外品を含む)、医薬品及び食品などには、防腐殺菌剤として、パラベン、安息香酸類、サリチル酸類等が用いられている。しかしながら、上記した防腐殺菌剤は皮膚刺激性が高く安全性が低いために使用濃度範囲が制限されるという欠点を有していた。例えば、パラベンや安息香酸塩の使用制限濃度は1%、安息香酸やサリチル酸の使用制限濃度は0.2%とされている。
また、上記した防腐殺菌剤はpHによる影響を受けやすいために、防腐殺菌効果の安定性が悪く、更に、界面活性剤などの他の配合成分との併用により、その防腐抗菌力が著しく低下する場合があるという問題も有していた。
BACKGROUND ART In cosmetics (including quasi-drugs), pharmaceuticals, foods, and the like, parabens, benzoic acids, salicylic acids, and the like are used as preservatives and disinfectants. However, the above-mentioned antiseptic disinfectant has a drawback that its use concentration range is limited due to high skin irritation and low safety. For example, the use limit concentration of paraben or benzoate is 1%, and the use limit concentration of benzoic acid or salicylic acid is 0.2%.
In addition, since the above-mentioned antiseptic disinfectant is easily affected by pH, the stability of the antiseptic disinfecting effect is poor, and further, in combination with other components such as a surfactant, the antiseptic antibacterial activity is significantly reduced. There was also a problem that there were cases.
近年、上記したような防腐殺菌剤に対してアレルギー反応を起こす人が増加する傾向にあり、安全性に対する指向がより高まって上記したような防腐殺菌剤を全く配合していないか、或いはその配合量を低減させた化粧品、医薬品及び食品などの需要が高まっている。 In recent years, the number of people who have an allergic reaction to the above-mentioned antiseptic disinfectant tends to increase, and the direction for safety has been further increased, and the above-mentioned antiseptic disinfectant has not been added at all, or its combination Demand for cosmetics, pharmaceuticals, foods, and the like with reduced amounts is increasing.
防腐殺菌剤を低減又は排除する技術として、1,2−アルカンジオールからなる防腐殺菌剤(特許文献1参照)や1,2−オクタンジオールからなる洗浄性又は非洗浄性化粧料用保湿静菌剤(特許文献2参照)などが開示されている。しかし、1,2−オクタンジオールなどの1,2−アルカンジオールを単独で防腐殺菌剤として用いた場合、充分な効果性を得るためには高配合量を必要とする場合があり、また、1,2−アルカンジオールは特有の原料臭を有することから低配合でも充分な防腐殺菌効果を発揮できる技術が望まれている。 As a technique for reducing or eliminating an antiseptic disinfectant, an antiseptic disinfectant comprising 1,2-alkanediol (see Patent Document 1) and a moisturizing bacteriostatic agent for detergency or non-detergency cosmetic comprising 1,2-octanediol (See Patent Document 2). However, when a 1,2-alkanediol such as 1,2-octanediol is used alone as an antiseptic, a high blending amount may be required in order to obtain sufficient effectiveness. Since 2,2-alkanediol has a peculiar raw material odor, there is a demand for a technique capable of exhibiting a sufficient antiseptic and sterilizing effect even at a low content.
また、1,2−アルカンジオールを用いた防腐殺菌剤に関する技術として、1,2−ペンタンジオールと2−フェノキシエタノールを組み合わせて配合した外用組成物(特許文献3参照)や1,2−アルカンジオールとパラベンからなる防腐殺菌剤(特許文献4参照)が開示されている。しかし、これらは、防腐剤と1,2−ペンタンジオールなどの1,2−アルカンジオールを併用して防腐剤の効果を向上させようとするものであって、防腐剤自体を完全に排除しようとするものではなく、また、1,2−アルカンジオールが有する抗菌力を増強させようとするものでもなかった。 In addition, as a technique relating to an antiseptic disinfectant using 1,2-alkanediol, an external composition (see Patent Document 3) in which 1,2-pentanediol and 2-phenoxyethanol are combined, and 1,2-alkanediol and An antiseptic disinfectant comprising paraben (see Patent Document 4) is disclosed. However, these are intended to improve the effect of the preservative by using a preservative in combination with a 1,2-alkanediol such as 1,2-pentanediol, and to completely eliminate the preservative itself. It was not intended to enhance the antibacterial activity of 1,2-alkanediol.
かかる実情に鑑み、本発明者らが鋭意研究をした結果、防腐殺菌剤として1,2−アルカンジオールとキレート剤とを共に使用することにより、1,2−アルカンジオールが本来有する抗菌活性を増強することを見出し、本発明を完成するに至った。 In view of such circumstances, the present inventors have conducted intensive research and have found that by using both a 1,2-alkanediol and a chelating agent as an antiseptic and bactericide, the antibacterial activity inherent in 1,2-alkanediol is enhanced. And completed the present invention.
即ち、請求項1に係る発明は、炭素数5〜10の1,2−アルカンジオールと、キレート剤とを含有することを特徴とする防腐殺菌剤に関する。
請求項2に係る発明は、前記1,2−アルカンジオールが、1,2−ヘキサンジオール及び/又は1,2−オクタンジオールであることを特徴とする請求項1に記載の防腐殺菌剤に関する。
請求項3に係る発明は、前記1,2−アルカンジオールが、1,2−オクタンジオールであることを特徴とする請求項1に記載の防腐殺菌剤に関する。
請求項4に係る発明は、前記キレート剤が、エデト酸、ポリリン酸、ピロリン酸、グルコン酸、及びそれらの塩からなる群から選択される1種以上であることを特徴とする請求項1乃至3のいずれかに記載の防腐殺菌剤に関する。
請求項5に係る発明は、前記キレート剤が、エデト酸、ポリリン酸、グルコン酸、及びそれらの塩からなる群から選択される1種以上であることを特徴とする請求項1乃至3のいずれかに記載の防腐殺菌剤に関する。
請求項6に係る発明は、請求項1乃至5のいずれかに記載の防腐殺菌剤を配合していることを特徴とする化粧品、医薬品または食品に関する。
That is, the invention according to claim 1 relates to an antiseptic disinfectant containing a 1,2-alkanediol having 5 to 10 carbon atoms and a chelating agent.
The invention according to claim 2 relates to the preservative disinfectant according to claim 1, wherein the 1,2-alkanediol is 1,2-hexanediol and / or 1,2-octanediol.
The invention according to claim 3 relates to the antiseptic disinfectant according to claim 1, wherein the 1,2-alkanediol is 1,2-octanediol.
The invention according to claim 4 is characterized in that the chelating agent is at least one selected from the group consisting of edetic acid, polyphosphoric acid, pyrophosphoric acid, gluconic acid, and salts thereof. 3. The antiseptic disinfectant according to any one of (3).
The invention according to claim 5 is characterized in that the chelating agent is at least one selected from the group consisting of edetic acid, polyphosphoric acid, gluconic acid, and salts thereof. And a preservative disinfectant according to the present invention.
The invention according to claim 6 relates to a cosmetic, pharmaceutical or food product containing the antiseptic disinfectant according to any one of claims 1 to 5.
本発明に係る防腐殺菌剤は、1,2−アルカンジオールとキレート剤とを含有するから、1,2−アルカンジオールが本来有する抗菌活性を増強する。よって、従来の防腐殺菌剤を配合する必要がなくなり、高い安全性を得ることもできる。 Since the antiseptic disinfectant according to the present invention contains a 1,2-alkanediol and a chelating agent, the antibacterial activity inherent to the 1,2-alkanediol is enhanced. Therefore, it is not necessary to mix a conventional antiseptic disinfectant, and high safety can be obtained.
1,2−アルカンジオールとして1,2−ヘキサンジオール又は1,2−オクタンジオールを用いると、より抗菌活性の高い防腐殺菌剤を得ることができる。
キレート剤としてエデト酸、ポリリン酸、グルコン酸、及びそれらの塩からなる群から選択される一種以上を用いると、より抗菌活性の高い防腐殺菌剤を得ることができる。
When 1,2-hexanediol or 1,2-octanediol is used as the 1,2-alkanediol, an antiseptic having a higher antibacterial activity can be obtained.
If one or more selected from the group consisting of edetic acid, polyphosphoric acid, gluconic acid, and salts thereof is used as a chelating agent, a preservative bactericide having higher antibacterial activity can be obtained.
本発明に係る防腐殺菌剤を配合した化粧品、医薬品及び食品は、サリチル酸、安息香酸、パラベンなどの従来の防腐殺菌剤を含有する必要がなく、しかも本発明に係る防腐殺菌剤は優れた抗菌活性を有しているので、防腐殺菌剤自体を低配合量とすることができ、極めて安全性の高いものである。 The cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant according to the present invention need not contain conventional antiseptic disinfectants such as salicylic acid, benzoic acid and paraben, and the antiseptic disinfectant according to the present invention has excellent antibacterial activity. Therefore, the antiseptic disinfectant itself can be used in a low blending amount, and is extremely safe.
以下、本発明に係る防腐殺菌剤について詳述する。本発明に係る防腐殺菌剤は、1,2−アルカンジオールとキレート剤とを含有する。 Hereinafter, the antiseptic disinfectant according to the present invention will be described in detail. The preservative disinfectant according to the present invention contains a 1,2-alkanediol and a chelating agent.
本発明に係る防腐殺菌剤の第一の成分は、次式1(化1)に示される炭素数5〜10の1,2−アルカンジオールであり、具体的には、1,2−ペンタンジオール、1,2−ヘキサンジオール、1,2−ヘプタンジオール、1,2−オクタンジオール、1,2−ノナンジオール、1,2−デカンジオールである。 The first component of the antiseptic disinfectant according to the present invention is a 1,2-alkanediol having 5 to 10 carbon atoms represented by the following formula (1), and specifically, 1,2-pentanediol. , 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, and 1,2-decanediol.
本発明では、前述の炭素数5〜10の1,2−アルカンジオールのうちの一種を単独で使用することもでき、二種以上を混合して用いることもできる。
1,2−アルカンジオールはそれ自体優れた抗菌力を有しており、本発明に係る防腐殺菌剤の抗菌力を高める効果を奏する。特に本発明では、炭素数5〜10の1,2−アルカンジオールのうち、1,2−ヘキサンジオール、1,2−オクタンジオールを用いることが好ましく、1,2−オクタンジオールを用いることがより好ましい。
In the present invention, one of the aforementioned 1,2-alkanediols having 5 to 10 carbon atoms can be used alone, or two or more can be used in combination.
1,2-Alkanediol itself has an excellent antibacterial activity, and exhibits an effect of increasing the antibacterial activity of the antiseptic disinfectant according to the present invention. In particular, in the present invention, among 1,2-alkanediols having 5 to 10 carbon atoms, it is preferable to use 1,2-hexanediol and 1,2-octanediol, and it is more preferable to use 1,2-octanediol. preferable.
本発明に係る防腐殺菌剤の第二の成分は、キレート剤である。
キレート剤は特に限定されず、例えば、エデト酸、ニトリロ三酢酸、ヒドロキシエチルエチレンジアミン三酢酸、ジエチレントリアミン五酢酸、トリエチレンテトラミン六酢酸、シクロヘキサンジアミン五酢酸、グリシン、セリン、アラニン、リジン、シスチン、チロシン、メチオニン、アスコルビン酸、クエン酸、リンゴ酸、酒石酸、乳酸、シュウ酸、マロン酸、マレイン酸、エリソルビン酸、コハク酸、フマル酸、ピルビン酸、サリチル酸、グルコン酸、ポリアクリル酸、ポリマレイン酸、ピロリン酸、トリポリリン酸、ポリリン酸及びオルトリン酸並びにこれらの塩等を例示することができる。
前記キレート剤の塩としては、ナトリウム塩、カリウム塩、カルシウム塩などを例示することができる。
キレート剤は一種を単独で配合してもよく、二種以上を混合して配合しても構わない。
The second component of the preservative disinfectant according to the present invention is a chelating agent.
Chelating agents are not particularly limited, for example, edetic acid, nitrilotriacetic acid, hydroxyethylethylenediaminetriacetic acid, diethylenetriaminepentaacetic acid, triethylenetetraminehexaacetic acid, cyclohexanediaminepentaacetic acid, glycine, serine, alanine, lysine, cystine, tyrosine, Methionine, ascorbic acid, citric acid, malic acid, tartaric acid, lactic acid, oxalic acid, malonic acid, maleic acid, erythorbic acid, succinic acid, fumaric acid, pyruvic acid, salicylic acid, gluconic acid, polyacrylic acid, polymaleic acid, pyrophosphoric acid , Tripolyphosphoric acid, polyphosphoric acid, orthophosphoric acid, and salts thereof.
Examples of the salt of the chelating agent include a sodium salt, a potassium salt, a calcium salt and the like.
One chelating agent may be used alone, or two or more chelating agents may be mixed and used.
キレート剤を炭素数5〜10の1,2−アルカンジオールとともに用いることにより、炭素数5〜10の1,2−アルカンジオールが有する抗菌作用を相乗的に高めることができる。特に、本発明では前述のキレート剤のうち、エデト酸、ポリリン酸、ピロリン酸、グルコン酸、及びそれらの塩のうちの一種又は二種以上が、より高い抗菌作用を得ることができるために好ましく、エデト酸、ポリリン酸、グルコン酸及びそれらの塩のうちの一種又は二種以上がより好ましく用いられる。 By using a chelating agent together with a 1,2-alkanediol having 5 to 10 carbon atoms, the antibacterial action of the 1,2-alkanediol having 5 to 10 carbon atoms can be synergistically enhanced. In particular, in the present invention, among the above-mentioned chelating agents, one or more of edetic acid, polyphosphoric acid, pyrophosphoric acid, gluconic acid, and salts thereof are preferable because a higher antibacterial action can be obtained. One or more of edetic acid, polyphosphoric acid, gluconic acid and salts thereof are more preferably used.
本発明に係る防腐殺菌剤において、第一の成分である炭素数5〜10の1,2−アルカンジオールと第二の成分であるキレート剤の含有量は特に限定されないが、重量比で1:100〜100:1、好ましくは1:10〜10:1となるように配合する。1,2−アルカンジオールをキレート剤の配合量の100重量倍を超えて配合すると、またキレート剤を1,2−アルカンジオールの配合量の100重量倍を超えて配合すると、ともに抗菌活性の増強効果が期待できなくなるために好ましくない。 In the antiseptic disinfectant according to the present invention, the content of the first component, a 1,2-alkanediol having 5 to 10 carbon atoms and the chelating agent, which is the second component, is not particularly limited. It is blended so as to be 100 to 100: 1, preferably 1:10 to 10: 1. When 1,2-alkanediol is added more than 100 times the amount of the chelating agent, and when the chelating agent is added more than 100 times the amount of the 1,2-alkanediol, the antibacterial activity is enhanced. It is not preferable because the effect cannot be expected.
本発明に係る防腐殺菌剤は、第一の成分である1,2−アルカンジオールと、第二の成分であるキレート剤とを含有するから、後述する試験例に示されるように、第一の成分と第二の成分との相乗効果によって、優れた防腐殺菌作用が発揮される。従って、パラベン、安息香酸、サリチル酸のような以前から用いられている防腐殺菌剤を低配合又は配合する必要がなくなり、極めて高い安全性を得ることもできる。 Since the antiseptic disinfectant according to the present invention contains a 1,2-alkanediol as a first component and a chelating agent as a second component, as shown in a test example described below, Due to the synergistic effect of the component and the second component, an excellent antiseptic and bactericidal action is exhibited. Therefore, it is not necessary to mix or use a conventionally used antiseptic disinfectant such as paraben, benzoic acid, and salicylic acid, and it is possible to obtain extremely high safety.
上述した本発明に係る防腐殺菌剤は、化粧品、医薬品及び食品などに配合して使用することができる。
具体的には、洗顔料、化粧水、乳液、クリーム、ファンデーション、マスカラ、ネールエナメル、口紅などの皮膚用化粧料、シャンプー、ヘアトリートメント、養毛・育毛料、ヘアクリーム、ヘアローション、ヘアフォーム、パーマネントウェーブ剤などの頭髪用化粧料、しみやそばかすなどの特定の使用目的を有した薬用化粧料(医薬部外品)、にきび治療薬、うがい薬、トローチなどの医薬品、さらにはチューインガム、キャンディー、飲料などの食品に好適に用いることができる。
The antiseptic disinfectant according to the present invention described above can be used by blending it in cosmetics, pharmaceuticals, foods, and the like.
Specifically, facial cleansers, lotions, emulsions, creams, foundations, mascara, nail enamel, lipsticks, and other skin cosmetics, shampoos, hair treatments, hair restoration / hair restoration, hair creams, hair lotions, hair foams, Hair cosmetics such as permanent waving agents, medicated cosmetics (quasi-drugs) with specific purposes such as spots and freckles, acne treatments, gargles, troches and other pharmaceuticals, as well as chewing gum, candy, It can be suitably used for foods such as beverages.
本発明に係る防腐殺菌剤を用いて化粧品、医薬品又は食品を調製する場合、本発明の効果が損なわれない範囲内で化粧品、医薬品又は食品に通常用いられる成分を適宜任意に配合することができる。例えば、化粧品や医薬品(医薬部外品を含む)の場合、油脂、ロウ類、高級脂肪酸、低級アルコール、高級アルコール、ステロール、脂肪酸エステル、保湿剤、界面活性剤、高分子化合物、無機顔料、色素、香料、酸化防止剤、紫外線吸収剤、ビタミン類、収斂剤、美白剤、動植物抽出物、精製水などを例示することができる。
また食品の場合は、動植物油、多糖類、甘味料、着色料、ガムベースなどを例示することができる。
When preparing cosmetics, pharmaceuticals or foods using the antiseptic disinfectant according to the present invention, components normally used in cosmetics, pharmaceuticals or foods can be appropriately mixed as long as the effects of the present invention are not impaired. . For example, in the case of cosmetics and pharmaceuticals (including quasi-drugs), oils and fats, waxes, higher fatty acids, lower alcohols, higher alcohols, sterols, fatty acid esters, humectants, surfactants, polymer compounds, inorganic pigments, pigments Fragrances, antioxidants, ultraviolet absorbers, vitamins, astringents, whitening agents, plant and animal extracts, purified water, and the like.
In the case of food, animal and vegetable oils, polysaccharides, sweeteners, coloring agents, gum bases and the like can be exemplified.
化粧品、医薬品又は食品を調製する場合、本発明に係る防腐殺菌剤の配合量は特に限定されないが、組成物中、0.01〜20重量%、好ましくは0.05〜5重量%配合する。20重量%を超えて配合したとしてもそれ以上の効果が望めない。また0.01重量%未満の場合は、抗菌効果が劣るために好ましくない。 When preparing cosmetics, pharmaceuticals or foods, the amount of the antiseptic disinfectant according to the present invention is not particularly limited, but is 0.01 to 20% by weight, preferably 0.05 to 5% by weight in the composition. Even if the content exceeds 20% by weight, no further effect can be expected. On the other hand, if the amount is less than 0.01% by weight, the antibacterial effect is inferior, so that it is not preferable.
[実施例1]
(供試菌)
供試菌は、Escherichia coli IFO3972(大腸菌)、Pseudomonas aeruginosa IFO13275(緑膿菌)及びCandida albicans IFO1594(口腔カンジダ症菌)を用いた。
[Example 1]
(Test bacteria)
As test bacteria, Escherichia coli IFO3972 (Escherichia coli), Pseudomonas aeruginosa IFO13275 (Pseudomonas aeruginosa) and Candida albicans IFO1594 (oral candidiasis) were used.
(接種用菌液の調製)
接種用菌液としては、大腸菌及び緑膿菌の場合、寒天培地で35℃で培養後、更にブイヨン培地に移植して35℃で培養した。得られた培養液をブイヨン培地で約108個/mLに希釈したものを接種用菌液とした。
また、口腔カンジダ症菌の場合、30℃で同様に培養して約107個/mLに希釈したものを接種用菌液とした。
(Preparation of bacterial solution for inoculation)
In the case of Escherichia coli and Pseudomonas aeruginosa, the inoculum was cultured at 35 ° C. on an agar medium, and then transplanted to a bouillon medium and cultured at 35 ° C. Those resulting broth was diluted to approximately 10 8 cells / mL in broth was inoculated for bacterial solution.
Also, in the case of oral candidiasis bacteria was inoculated for bacterial solution diluted to about 107 cells / mL were cultured in the same manner at 30 ° C..
(被検物質の希釈系列の調製)
20w/w%エチルセルソルブを希釈溶媒とし、5、4、3、2.5、2.25、2、1.75、1.5、1.25、1w/v%の1,2−オクタンジオール液を調製した。
また、エデト酸二ナトリウム及び1,2−オクタンジオールとエデト酸二ナトリウムの等量混合物については、5w/v%の液を倍倍希釈して希釈系列を調製した。
(Preparation of dilution series of test substance)
5,4,3,2.5,2.25,2,1.75,1.5,1.25,1w / v% 1,2-octane using 20 w / w% ethyl cellosolve as a diluting solvent A diol solution was prepared.
Further, with respect to disodium edetate and an equal mixture of 1,2-octanediol and disodium edetate, a dilution series was prepared by diluting a 5% w / v% solution twice.
(最小発育阻止濃度(MIC)の測定)
上記被検物質を含む希釈系列1mLに対して各寒天培地9mLをシャーレに入れ、それぞれについて、上記接種用菌液を約1cmの長さに画線した。培養は、大腸菌及び緑膿菌については35℃で行い、2日後の菌の生育の有無を判定した。また、口腔カンジダ症菌については25℃で培養を行い、3日後の菌の生育の有無を判定した。このとき、生育が認められなかった最小の濃度をMICとして求めた。結果を表1に示す。
(Measurement of minimum growth inhibitory concentration (MIC))
9 mL of each agar medium was placed in a Petri dish with 1 mL of the dilution series containing the test substance, and the bacterial solution for inoculation was streaked to a length of about 1 cm for each. Culture was performed at 35 ° C. for Escherichia coli and Pseudomonas aeruginosa, and the presence or absence of growth of the bacteria two days later was determined. Moreover, about the oral candidiasis bacteria, it culture | cultivated at 25 degreeC and the presence or absence of growth of the bacteria after 3 days was determined. At this time, the minimum concentration at which no growth was observed was determined as MIC. Table 1 shows the results.
尚、MICによって、抗菌力を評価することができる。被検物質の濃度が薄いときには微生物への影響はないが、濃度を増していくと発育抑制が起こる。この程度は、濃度に依存して発育抑制が進み、ついには発育が停止する。このときの濃度がMICとして表される。したがって、MIC以上の濃度になると、微生物は死滅していくことになる。 The antimicrobial activity can be evaluated by MIC. When the concentration of the test substance is low, there is no effect on the microorganism, but as the concentration increases, the growth is inhibited. To this extent, growth suppression is advanced depending on the concentration, and growth is eventually stopped. The concentration at this time is expressed as MIC. Therefore, when the concentration becomes higher than the MIC, the microorganisms will die.
(二元最小発育阻止濃度)
得られた1,2−オクタンジオール、エデト酸二ナトリウム、及び1,2−オクタンジオールとエデト酸二ナトリウムの等重量混合物の各MICを、1,2−オクタンジオール及びエデト酸二ナトリウムの配合量に対してプロットし、二元最小発育阻止濃度図を求めた。
尚、二元最小発育阻止濃度により、抗菌性を有する二種類の物質を配合した場合の作用効果を判定することができる。具体的には、抗菌性を有する二種類の物質を配合した場合、それにより生ずる作用は、相乗作用、相加作用、拮抗作用に大別される。相乗作用とは、二薬剤が相乗的に作用し、本来有する抗菌力が更に増強される作用である。相加作用とは、各薬剤の抗菌力が合わさった作用である。拮抗作用とは、一薬剤が他剤の抗菌力を打ち消す場合の作用である。そして、二元最小発育阻止濃度図による方法は、例えば、図1に示すように、A物質とB物質について、それぞれの割合を変えてMICを測定し、グラフから判定する方法である。これによると、A物質のみにおけるMIC(点A)とB物質のみにおけるMIC(点B)とをプロットした点を結び、両物質を併用したときのMICが、この線上より内側にある場合(点C)は、併用により抗菌力が増強された相乗作用であると、線上(点D)にある場合は相加作用であると、線上より外側にある場合(点E)は、一方又は双方の抗菌力を打ち消し抗菌力を減少させる拮抗作用であると判定することができる。
図2にエデト酸二ナトリウムに関する二元最小発育阻止濃度図を示す。
(Binary minimum inhibitory concentration)
Each MIC of the obtained 1,2-octanediol, disodium edetate, and an equal weight mixture of 1,2-octanediol and disodium edetate was used as the blending amount of 1,2-octanediol and disodium edetate. , And a binary minimum growth inhibitory concentration diagram was obtained.
The action and effect when two kinds of substances having antibacterial properties are blended can be determined from the binary minimum growth inhibitory concentration. Specifically, when two kinds of substances having antibacterial properties are blended, the resulting action is roughly classified into a synergistic action, an additive action, and an antagonistic action. The synergistic action is an action in which the two drugs act synergistically to further enhance the intrinsic antibacterial activity. The additive action is an action obtained by combining the antibacterial activities of the respective drugs. Antagonistic action is the action when one drug counteracts the antimicrobial activity of another drug. The method based on the binary minimum growth inhibition concentration diagram is, for example, a method of measuring the MIC of the substance A and the substance B at different ratios as shown in FIG. According to this, a point obtained by plotting the MIC of only the substance A (point A) and the MIC of the substance B only (point B) is connected, and the MIC when both substances are used together is inside the line (point C) is a synergistic effect in which the antibacterial activity is enhanced by the combined use, if it is on the line (point D), it is an additive effect, and if it is outside the line (point E), it is one or both. It can be determined that the antimicrobial activity is an antagonistic action that counteracts the antibacterial activity and decreases the antibacterial activity.
FIG. 2 shows a binary minimum inhibitory concentration diagram for disodium edetate.
[実施例2]
キレート剤としてポリリン酸ナトリウムを、供試菌として緑膿菌及び口腔カンジダ症菌を用いて実施例1と同様に操作し、表2のMIC及び図3の二元最小発育阻止濃度図を求めた。
[Example 2]
The same operation as in Example 1 was carried out using sodium polyphosphate as a chelating agent and Pseudomonas aeruginosa and oral candidiasis as test bacteria, and the MIC in Table 2 and the binary minimum growth inhibitory concentration diagram in FIG. 3 were obtained. .
[実施例3]
キレート剤としてグルコン酸ナトリウムを、供試菌としてStaphylococcus aureus IFO13276(黄色ブドウ状球菌)を用いて、実施例1の大腸菌の場合と同様に操作し、表3のMIC及び図4の二元最小発育阻止濃度図を求めた。
[Example 3]
Using sodium gluconate as a chelating agent and Staphylococcus aureus IFO13276 (Staphylococcus aureus) as a test bacterium, the same operation as in the case of Escherichia coli of Example 1 was performed, and the MIC of Table 3 and the binary minimum growth of FIG. An inhibitory concentration diagram was determined.
(抗菌効果の評価)
図2〜図4の結果から、1,2−オクタンジオールと、エデト酸二ナトリウム、ポリリン酸ナトリウム、グルコン酸ナトリウム等のキレート剤との組合せにより、1,2−オクタンジオールが本来有する抗菌活性が相乗的に増強されることが分かる。
(Evaluation of antibacterial effect)
From the results of FIGS. 2 to 4, the combination of 1,2-octanediol and a chelating agent such as disodium edetate, sodium polyphosphate, and sodium gluconate shows that the antibacterial activity inherent in 1,2-octanediol is It can be seen that they are synergistically enhanced.
以下、本発明に係る防腐殺菌剤を配合した化粧品、医薬品及び食品の配合例を示す。
<処方例1:保湿クリーム>
モノラウリン酸デカグリセリル 1.0
モノステアリン酸POE(15)グリセリル 1.0
水素添加大豆リン脂質 1.0
ステアリン酸 4.0
セタノール 2.0
ベヘニルアルコール 2.0
パラフィン 3.0
スクワラン 12.0
ホホバ油 4.0
メチルポリシロキサン 0.2
1,3−ブチレングリコール 3.0
L−アルギニン 0.1
キサンタンガム 0.001
1,2−オクタンジオール 0.25
エデト酸二ナトリウム 0.10
精製水 適 量
合計 100.0重量%
Hereinafter, examples of blending cosmetics, pharmaceuticals, and foods containing the antiseptic disinfectant according to the present invention will be described.
<Prescription example 1: moisturizing cream>
Decaglyceryl monolaurate 1.0
POE (15) glyceryl monostearate 1.0
Hydrogenated soybean phospholipid 1.0
Stearic acid 4.0
Cetanol 2.0
Behenyl alcohol 2.0
Paraffin 3.0
Squalane 12.0
Jojoba oil 4.0
Methyl polysiloxane 0.2
1,3-butylene glycol 3.0
L-arginine 0.1
Xanthan gum 0.001
1,2-octanediol 0.25
Disodium edetate 0.10
Purified water qs
100.0% by weight in total
<処方例2:親水性軟膏>
アスコルビン酸 0.5
ポリオキシエチレンセチルエーテル 2.0
水素添加大豆リン脂質 1.0
ステアリン酸 4.0
グリセリンモノステアレート 10.0
流動パラフィン 10.0
ワセリン 4.0
セタノール 5.0
プロピレングリコール 5.0
1,2−ヘキサンジオール 0.5
ポリリン酸ナトリウム 0.2
精製水 適 量
合計 100.0重量%
<Prescription example 2: hydrophilic ointment>
Ascorbic acid 0.5
Polyoxyethylene cetyl ether 2.0
Hydrogenated soybean phospholipid 1.0
Stearic acid 4.0
Glycerin monostearate 10.0
Liquid paraffin 10.0
Vaseline 4.0
Cetanol 5.0
Propylene glycol 5.0
1,2-hexanediol 0.5
Sodium polyphosphate 0.2
Purified water qs
100.0% by weight in total
<処方例3:飲料>
ブドウ糖液糖 33.0
グレープフルーツ果汁 64.0
1,2−ペンタンジオール 0.5
ポリリン酸ナトリウム 0.01
香料 0.5
酸味料 適 量
合計 100.0重量%
<Formulation Example 3: Beverage>
Glucose liquid sugar 33.0
Grapefruit juice 64.0
1,2-pentanediol 0.5
Sodium polyphosphate 0.01
Fragrance 0.5
Acidulant qs
100.0% by weight in total
本発明に係る防腐殺菌剤は、炭素数5〜10の1,2−アルカンジオールとキレート剤とを併用することにより、1,2−アルカンジオールが本来有する防腐殺菌活性を相乗的に増強することができるので、化粧品、医薬品又は食品などの防腐殺菌剤として有用である。
本発明に係る化粧品、医薬品又は食品は、防腐殺菌剤として、炭素数5〜10の1,2−アルカンジオールと、キレート剤とが含有されているので、従来の防腐殺菌剤の配合量を低配合量又は実質的に無配合量とすることができ、高い安全性を有する化粧品、医薬品又は食品として有用である。
The preservative disinfectant according to the present invention synergistically enhances the preservative disinfecting activity inherent in 1,2-alkanediol by using a 1,2-alkanediol having 5 to 10 carbon atoms and a chelating agent in combination. Therefore, it is useful as an antiseptic disinfectant for cosmetics, pharmaceuticals, foods, and the like.
The cosmetic, pharmaceutical or food according to the present invention contains a 1,2-alkanediol having 5 to 10 carbon atoms and a chelating agent as preservatives, so that the amount of the conventional preservatives is reduced. It can be used in a blended amount or a substantially non-blended amount, and is useful as cosmetics, pharmaceuticals or foods having high safety.
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