CN1322815C - Method for preparing the bactericide and the cleansing facial milk] - Google Patents
Method for preparing the bactericide and the cleansing facial milk] Download PDFInfo
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- CN1322815C CN1322815C CNB021453314A CN02145331A CN1322815C CN 1322815 C CN1322815 C CN 1322815C CN B021453314 A CNB021453314 A CN B021453314A CN 02145331 A CN02145331 A CN 02145331A CN 1322815 C CN1322815 C CN 1322815C
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- mildy wash
- bactericide
- surfactant
- lysozyme
- modified silicone
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Abstract
The present invention provides a germicide comprising lysostaphin and lysozyme, a facial washing milk using the germicide as a main active component and a preparation method thereof. The germicide has the characteristics of strong bactericidal activity, mild action to skin, no irritant to skin, etc., and thus, the facial washing milk has the functions of cleaning, moisture retention, nourishment, moistening, etc. to skin; what is more important, the facial washing milk has strong killing effect on facial bacteria, prevents the infection of acne bacillus without irritant to skin and is a novel facial washing milk.
Description
Technical field
The present invention relates to a kind of bactericide, a kind of mildy wash and preparation method thereof specifically relates to a kind of biological bactericide and contains mildy wash of this biological bactericide and preparation method thereof.
Background technology
Mildy wash adopts the mode of washing to clean the emulsion form product of human body face skin exactly.At present the basis of mildy wash commonly used is water and surfactant, add additives such as essence, humectant, preservative, antioxidant with stable prod, compose with its fragrance.Its decontamination, cleaning action comprise it being wetting, osmosis by means of surfactant, make facial dirt be easy to come off, and with dirt emulsification, are scattered in the water then.
Though yet surfactant has good cleaning action, can not effectively kill and bacterium that portion hides, contain certain nutrient component toward contact because of it, make the Propiobacterium of in cleaning skin nutrition face, small pox is long more many more.
And the bacillus acnes that exists in the hair follicle of skin, staphylococcus albus and oval pityriasis sporeformer, particularly bacillus acnes contains the esterase that sebum is decomposed, intrafollicular sebum is decomposed by lipase and produces more free fatty acid, non-particularity inflammatory reaction takes place around making hair follicle and hair follicle in these free fatty acid, near the atomic ulcer of acne wall and free fatty acid enter behind the corium, add near the cell of blackhead extruding, make their antimicrbial power decline and caused inflammation by the infection of bacterium, so papule takes place in patient, warts, rhabdion, tubercle and abscess.
At present commercially available mildy wash kind is a lot, and its function mostly is cleaning, preserves moisture, skin moisten, whiten etc., and does not have sterilizing function.Mildy wash that has such as CN1148489A and CN1148490A, though certain bacteriostasis is arranged, good kill bacteria, and skin had certain excitant.Thereby develop and a kind ofly can effectively clean, preserve moisture, moisten skin of face, can effectively kill again the facial infection bacterium and to the skin action temperature and, non-irritating mildy wash, be the needs of existing market.
Summary of the invention
Problem to be solved by this invention is to provide a kind of biological bactericide to reach mildy wash that is mixed with by this bactericide and preparation method thereof, can not effectively remove facial bacterium, the shortcoming strong to skin irritation with mildy wash in the solution prior art.
The present invention at first provides a kind of bactericide, and this bactericide comprises staphylococcus lysozyme and lysozyme, and wherein the weight ratio of staphylococcus lysozyme and lysozyme is 1: 100-1: 400, and preferred ratio is 1: 200.
The present invention also provides a kind of mildy wash, this mildy wash is to be main fungicide active ingredient with above-mentioned bactericide, comprise surfactant simultaneously with cleaning action, surfactant be mainly action temperature and amphoteric surfactant, anion surfactant or non-ionic surface active agent.The component and the weight percent content of mildy wash comprise:
Bactericide 0.0001-0.1%
Surfactant 5-20%
Water surplus.
Staphylococcus lysozyme and activity of lysozyme unit's ratio are 1: 200;
Preferred ingredients and weight percent content comprise:
Bactericide 0.001-0.01
Surfactant 10-15%
Water surplus.
Preferred surfactants comprises one or more in imidazolinium betaine, fatty alcohol-polyoxyethylene ether, cocoa butter single ethanol amide, Cocoamidopropyl betaine, cocamidopropyl propyl amide amine oxide, trialkyl methyl oxidation amine, castor oil polyoxyethylene ether or the hydroxy sulfo lycine.
This mildy wash also comprises one or more in humectant, nutritional agents, emollient or the excipient.Wherein preferred humectant comprises one or more in propane diols, polyethylene glycols, glycerine or the sorbierite; Preferred nutritional agents comprises one or more in vitamin or the collagen; Preferred emollient comprises one or more in water-soluble modified silicone oil, cation-modified silicone oil, amino-modified silicone oil and the amino composite modified silicone oil; Preferred excipient comprises one or more in polyvinylpyrrolidone, two stearic acid polyethylene glycol or the cationic cellulose.
The present invention also provides the preparation method of this mildy wash, and surfactant, humectant etc. is dissolved in the solvent about 70 ℃, stirs, and is cooled to 40-50 ℃ then, adds bactericide, stirs, and gets final product.Said process all carries out under germ-free condition.
Characteristics of the present invention:
1. bactericide of the present invention is the purpose that reaches sterilization with the glycine peptide bond in the cracking bacteria cell wall, thereby sterilizing ability is strong, effective (bactericidal effect sees Table 1-2), bacterium is difficult for producing drug resistance, and because of its main component is a biologic product, to body, mucocutaneous without any side effects.
2. compositions such as bactericide and surfactant, humectant, emollient are carried out the composite mildy wash that forms, supplementary element not only makes biotype bactericide stability strengthen, help preserving (stability test sees Table 3), and make its bactericidal activity enhancing be original 2-10 times.
Effective cleaning, preserve moisture, moisten, skin-protecting function, and can strengthen skin elasticity;
To the skin action temperature and, nonirritant;
5. after skin absorbs, because be biology enzyme and can not cause any toxic and side effect to body.
Shanghai Disease Prevention and Control Centre is detected every index of this mildy wash:
1. basic the detection
Micro biological Tests: national health requirement
Total number of bacteria (CFU/g): must not be greater than 1000 less than 10
Mould and saccharomycete sum (CFU/g): must not be greater than 100 less than 10
Staphylococcus aureus: must not detect and detect
Fecal coliform number: must not detect and detect
Pseudomonas aeruginosa: must not detect and detect
Chemical analysis:
Plumbous (in lead) (mg/kg):＜2.6 must not surpass 40
Arsenic (in arsenic) is (mg/kg):＜0.02 must not surpass 10
Mercury (mg/kg):＜0.02 must not surpass 1
2. press down bactericidal effect
The fungistatic effect of table 1 pair staphylococcus aureus (ATCC6538)
| The test sequence number | The inhibition zone diameter of each print (mm) | Negative control inhibition zone diameter (mm) | ||||
| 1 | 2 | 3 | 4 | Mean value | ||
| 1 2 3 | 25 20 22 | 22 20 22 | 24 22 21 | 24 22 21 | 23.75 21 21.5 | 0 0 0 |
Annotate: test is 5.5 * 10 with the concentration and the scope of bacteria suspension
5Cfu/ml.
The fungistatic effect of table 2 pair Candida albicans (ATCC10231)
| The test sequence number | The inhibition zone diameter of each print (mm) | Negative control inhibition zone diameter (mm) | ||||
| 1 | 2 | 3 | 4 | Mean value | ||
| 1 2 3 | 17 15 16 | 17 15 16 | 15 19 14 | 15 19 14 | 16 17 15 | 0 0 0 |
Annotate: test is 5.9 * 10 with the concentration and the scope of bacteria suspension
5Cfu/ml.
3. stability test
Preservation condition: 37 ℃ of incubators (relative temperature＞75%) three months
The fungistatic effect of table 3 pair staphylococcus aureus (ATCC6538)
| The test sequence number | The inhibition zone diameter of each print (mm) | Negative control inhibition zone diameter (mm) | ||||
| 1 | 2 | 3 | 4 | Mean value | ||
| 1 2 3 | 20 20 21 | 20 18 22 | 20 18 22 | 20 18 22 | 20 18.5 21.75 | 0 0 0 |
Annotate: test is 5.5 * 10 with the concentration and the scope of bacteria suspension
5Cfu/ml.
4. toxicology detects
4.1 many skin irritation tests: every day, every animal skin irritant reaction average product score value was 0.3, belonged to nonirritant.
4.2 acute eye irritation test: animal left side eyelid is separated gently, will be tried thing 0.1ml and splash in the conjunctival sac, incite somebody to action catacleisis 4-10s up and down, the right side eyelid splashes into physiological saline and compares.Do not wash in 24 hours behind first group of eye drip, second, third group is observed then respectively at 4s behind the eye drip or 30s flushing.Result: do not wash group: it is that 25.5, the 7 days eye stimulation total mark mean value is 1.0 that the preceding 4 days eyes in contamination back stimulate top mean value, belongs to the moderate excitant; It is that 4.7, the 2 days eye stimulation total mark mean value is 2.7 that the preceding 4 days eyes in 4s flushing group contamination back stimulate top mean value, belongs to slight excitant; It is that 11.0, the 2 days eye stimulation total mark mean value is 5.7 that the preceding 4 days eyes in 30s flushing group contamination back stimulate top mean value, belongs to slight excitant.
Embodiment
Embodiment 1 (preparation 100g mildy wash):
Bactericide 0.0001
Imidazolinium betaine 3%
Trialkyl methyl oxidation amine 5%
Glycerine 3%
Sorbierite 1%
Preservative card pine CG 0.05%
Water surplus
To according to above-mentioned content ratio, be dissolved in the deionized water about 70 ℃ except that the component of bactericide under aseptic condition, stir, be cooled to 40-50 ℃ then, add bactericide, stir, can gets final product.
Embodiment 2 (preparation 100g mildy wash)
Bactericide 0.001
Imidazolinium betaine 3%
Trialkyl methyl oxidation amine 5%
Cetomacrogol 1000 3%
Sorbierite 1%
Preservative card pine CG 0.05%
Water surplus
To according to above-mentioned content ratio, be dissolved in the deionized water about 70 ℃ except that the component of bactericide under aseptic condition, stir, be cooled to 40-50 ℃ then, add bactericide, stir, can gets final product.
Embodiment 3 (preparation 100g mildy wash)
Bactericide 0.005
Imidazolinium betaine 6%
Fatty alcohol-polyoxyethylene ether 3%
Cocoa butter single ethanol amide 4%
Polyethylene glycol 1500 3%
Glycerine 2%
Vit C 1％
Vit E 1％
Water surplus
To according to above-mentioned content ratio, be dissolved in the deionized water about 70 ℃ except that the component of bactericide under aseptic condition, stir, be cooled to 40-50 ℃ then, add bactericide, stir, can gets final product.
Embodiment 4 (preparation 100g mildy wash)
Bactericide 0.008
Cocamidopropyl propyl amide amine oxide 4%
Imidazolinium betaine 5%
Propane diols 2%
Glycerine 2%
Polyvinylpyrrolidone 4%
Vit E 1％
Water surplus
To according to above-mentioned content ratio, be dissolved in the deionized water about 70 ℃ except that the component of bactericide under aseptic condition, stir, be cooled to 40-50 ℃ then, add bactericide, stir, can gets final product.
Embodiment 5 (preparation 100g mildy wash)
Bactericide 0.01
Cocoamidopropyl betaine 5%
Glycerine 3%
Sorbierite 3%
Cation-modified silicone oil 3%
Cationic fiber 1%
Collagen 2%
Water surplus
To according to above-mentioned content ratio, be dissolved in the deionized water about 70 ℃ except that the component of bactericide under aseptic condition, stir, be cooled to 40-50 ℃ then, add bactericide, stir, can gets final product.
Embodiment 6 (preparation 100g mildy wash)
Bactericide 0.05
Cocoamidopropyl betaine 5%
Imidazolinium betaine 2%
Glycerine 3%
Sorbierite 3%
Cation-modified silicone oil 3%
Cationic fiber 1%
Collagen 2%
Water surplus
To according to above-mentioned content ratio, be dissolved in the deionized water about 70 ℃ except that the component of bactericide under aseptic condition, stir, be cooled to 40-50 ℃ then, add bactericide, stir, can gets final product.
Claims (8)
1. mildy wash is characterized in that component and weight percent content comprise:
Bactericide 0.0001-0.1%
Surfactant 5-20%
Water surplus
Bactericide is staphylococcus lysozyme and lysozyme, and the weight ratio of staphylococcus lysozyme and lysozyme is 1: 100-1: 400, and staphylococcus lysozyme and activity of lysozyme unit's ratio are 1: 200;
Described surfactant is one or more in amphoteric surfactant, anion surfactant or the non-ionic surface active agent.
2. mildy wash as claimed in claim 1 is characterized in that component and weight percent content comprise:
Bactericide 0.001-0.01%
Surfactant 10-15%
Water surplus.
3. mildy wash as claimed in claim 1 or 2 is characterized in that described surfactant comprises one or more in imidazolinium betaine, fatty alcohol-polyoxyethylene ether, cocoa butter single ethanol amide, Cocoamidopropyl betaine, cocamidopropyl propyl amide amine oxide, trialkyl methyl oxidation amine, castor oil polyoxyethylene ether or the hydroxy sulfo lycine.
4. mildy wash as claimed in claim 3 is characterized in that this mildy wash also comprises one or more in humectant, nutritional agents, emollient or the excipient.
5. mildy wash as claimed in claim 4 is characterized in that described humectant comprises one or more in propane diols, polyethylene glycols, glycerine or the sorbierite.
6. mildy wash as claimed in claim 4 is characterized in that described nutritional agents comprises one or more in vitamin or the collagen.
7. mildy wash as claimed in claim 4 is characterized in that described emollient comprises one or more in water-soluble modified silicone oil, cation-modified silicone oil, amino-modified silicone oil and the amino composite modified silicone oil.
8. mildy wash as claimed in claim 4 is characterized in that described excipient comprises one or more in polyvinylpyrrolidone, two stearic acid polyethylene glycol or the cationic cellulose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021453314A CN1322815C (en) | 2002-11-21 | 2002-11-21 | Method for preparing the bactericide and the cleansing facial milk] |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021453314A CN1322815C (en) | 2002-11-21 | 2002-11-21 | Method for preparing the bactericide and the cleansing facial milk] |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1415209A CN1415209A (en) | 2003-05-07 |
| CN1322815C true CN1322815C (en) | 2007-06-27 |
Family
ID=4750856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021453314A Expired - Lifetime CN1322815C (en) | 2002-11-21 | 2002-11-21 | Method for preparing the bactericide and the cleansing facial milk] |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1322815C (en) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1309304C (en) * | 2003-06-06 | 2007-04-11 | 上海高科联合生物技术研发有限公司 | Sterilizing gel, preparing method and application thereof |
| CN101507700B (en) * | 2008-03-12 | 2011-02-02 | 北京绿岛缘蜂产品有限公司 | High-efficiency sterilization shower cream |
| CN101601380B (en) * | 2009-07-16 | 2013-04-10 | 杨连孝 | Special-purpose emulsifying thickener for paraquat preparation and application method thereof |
| CN102552072B (en) * | 2012-02-17 | 2013-07-31 | 石家庄诺利达医疗器械有限公司 | Hand wash for children |
| CN103893027A (en) * | 2012-12-25 | 2014-07-02 | 青岛海芬海洋生物科技有限公司 | Shower gel containing marine biological ingredient and preparing method thereof |
| CN103027857B (en) * | 2012-12-25 | 2014-04-09 | 青岛海芬海洋生物科技有限公司 | Shampoo containing marine biological degerming components and preparation method of shampoo |
| CN104706551A (en) * | 2013-12-13 | 2015-06-17 | 江南大学 | Facial cleanser containing ampelopsin grossedentata extract |
| CN103877569B (en) * | 2014-04-14 | 2016-01-06 | 李志臣 | A kind of compound lysostaphin enzyme disinfectant |
| CN106389199A (en) * | 2016-10-06 | 2017-02-15 | 刘艺鹏 | Herbal inflammation-diminishing acne-removing facial cleanser |
| CN106726682A (en) * | 2016-12-09 | 2017-05-31 | 申艾平 | A kind of mildy wash and preparation method thereof |
| CN107320358A (en) * | 2017-08-25 | 2017-11-07 | 安庆徽云博冠化妆品科技有限公司 | A kind of Ai Xiang mildy wash and preparation method thereof |
| CN115444779A (en) * | 2022-09-14 | 2022-12-09 | 昆山博青生物科技有限公司 | Bactericidal composition, biological enzyme functional bath lotion prepared by applying bactericidal composition and preparation method of biological enzyme functional bath lotion |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0359873A1 (en) * | 1988-08-29 | 1990-03-28 | Applied Microbiology, Inc. | Method of treating mastitis and other staphylococcal infections |
| CN1327741A (en) * | 2000-06-08 | 2001-12-26 | 上海高科生物工程有限公司 | Compound lysostaphin enzyme disinfectant |
-
2002
- 2002-11-21 CN CNB021453314A patent/CN1322815C/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0359873A1 (en) * | 1988-08-29 | 1990-03-28 | Applied Microbiology, Inc. | Method of treating mastitis and other staphylococcal infections |
| CN1327741A (en) * | 2000-06-08 | 2001-12-26 | 上海高科生物工程有限公司 | Compound lysostaphin enzyme disinfectant |
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| Publication number | Publication date |
|---|---|
| CN1415209A (en) | 2003-05-07 |
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Granted publication date: 20070627 |