KR100513301B1 - A composition for washing foot - Google Patents

Abstract

The present invention relates to a composition for washing feet, and more particularly, to a composition for washing feet containing keratin degrading enzymes and exfoliants. The composition of the present invention may further comprise a ceratiopeptidase or natural substance having an anti-inflammatory action. The keratin degrading enzymes can decompose keratin, the main component of the stratum corneum, induce a synergistic effect on the exfoliation and promote antimicrobial effect and metabolism to help exfoliation, cleanliness, skin protection and health, of the present invention The composition can be usefully used as a foot cleanser by showing excellent antimicrobial effect over pathogens including gram positive bacteria, gram negative bacteria, and molds.

Description

A composition for foot washing {A COMPOSITION FOR WASHING FOOT}

The present invention relates to a composition for washing feet, and more particularly, to a composition for washing feet containing an exfoliating agent and keratin degrading enzyme.

Skin is largely classified into epidermis and dermis. The epidermis is composed of horney layer, clear or lucid layer, granular layer and germinal layer. It is composed of cutaneus n. And blood vessels.

The stratum corneum is composed of about 50% keratin, about 20% fat, about 23% water-soluble substance, about 7% moisture, and other ingredients, and is a part where the cells that reach the outer surface of the skin are hardened and formed. They act as a defense against the release of substances in the body and protect the body from external physical, chemical and biological stimuli. The keratinocytes produced in the basal layer pass through the granule layer and migrate to the stratum corneum. Epidermal cells that reach the stratum corneum lose their nuclei and are converted to dead cells as they are filled with an insoluble protein called keratin. The keratinocytes are connected to each other by a protein called desmozom, and as they rise up the stratum corneum, desmosomes are degraded to weaken the cohesion between keratinocytes and eventually the keratinocytes are separated from the skin.

The time it takes to pass through multiple layers in the production of keratinocytes is called transit time. The travel time from the basal layer to the granular layer is 14 days, and 14 days until it reaches the stratum corneum from the granular layer to reach the stratum corneum. In the production of keratinocytes, desorption takes 28 days.

In recent years, attempts have been made to accelerate the turnover rate of the stratum corneum and to easily detach the aged keratinocytes to improve the whitening effect or skin elasticity.

Korean Patent Publication No. 300197 discloses a cosmetic composition for feet containing glycolic acid, an alpha hydroxy acid having an exfoliating function, and an element having a moisturizing and exfoliating function, and Korean Patent Laid-Open Publication No. 2000-44978 discloses It discloses a preparation for promoting keratin exfoliation containing isoserine (3-amino-2-hydroxypropionic acid) that acts on the hairsome to promote exfoliation, and Korean Patent Publication No. 2000-66979 discloses Bacillus subtilis ( A human detergent composition comprising an enzyme solution obtained from Bacillus subtilis ) is disclosed. And, Korean Patent No. 2000-14115 discloses a rubbing cream composition for exfoliation, the composition is a proteolytic enzyme bromelain (Bromelain) obtained from pineapple stem and papain (protease obtained from papaya tree) papain). According to the above document, they tried to effectively achieve exfoliation by acting on desmosome, an intercellular adhesion material.

However, the above examples did not exhibit a rinse action by sufficient exfoliation, and therefore, a foot cleaner having good performance was required. Accordingly, the present inventors confirmed that the addition of keratin degrading enzyme to the foot washing composition including the exfoliating agent increases the exfoliation effect and the antimicrobial activity also increases, thereby completing the present invention.

Accordingly, the present invention is to provide a new foot cleaning composition with improved effect of the foot cleaning.

Another object of the present invention is to provide a composition for washing feet excellent in antibacterial action.

Another object described above and in the detailed description of the invention can be achieved by the additional supply of keratin degrading enzymes to conventional compositions comprising the exfoliating agent.

The present invention relates to a composition for cleansing feet comprising an exfoliant and keratin degrading enzyme.

As used herein, the term "exfoliant" refers to a substance having an exfoliating function, and includes both a substance serving to exfoliate a keratin or a substance acting on a desmosome to promote detachment of keratinocytes. Examples of the former include alpha hydroxy acid, beta hydroxy acid or urea. Examples of the latter include isoserine (3-amino-2-hydroxypropionic acid), bromelain and papain. Etc. can be mentioned.

As used herein, the term "keratin degrading enzyme" refers to an enzyme that degrades keratin proteins, which are the main components of the stratum corneum, and these can be easily separated from microorganisms. The keratin degrading enzyme decomposes keratin, induces softening of the stratum corneum, and plays a role of promoting keratin removal by an exfoliating agent, as well as Gram-negative bacteria. It exhibits antimicrobial activity against pathogens such as Gram-positive bacteria and fungi. The keratin degrading enzyme for use in the present invention is stable at a temperature of 25 ° C to 40 ° C and a pH of 4.0 to 9.0, an optimum reaction temperature of 30 to 37 ° C, and an optimum pH of 5.0 to 7.0 are suitable. The keratin degrading enzyme can be used in the form of a liquid state, a concentrated liquid concentrate in a liquid state, or a dry preparation. The stratum corneum of the sole lacks sebaceous glands present in the sweat glands, and fatty acids are not secreted and do not have a bactericidal action. Therefore, keratin degrading enzyme excellent in antimicrobial activity can solve this problem. Preferable amount of use is 0.01 to 1.00 weight% with respect to the whole composition, More preferably, it is 0.03 to 0.05 weight%. When used in less than 0.01% by weight, the protein resolution is lowered, and when used in excess of 1.00% by weight can cause problems of excessive exfoliation due to overuse.

The composition of the present invention may further include a natural substance having an antimicrobial effect and a function to help metabolism, and examples thereof include humbac, mugwort, green tea, ogapi, ginseng, garlic, and ginkgo biloba. The natural substance not only increases the antibacterial activity of keratinase, but also promotes metabolism and serves to improve skin elasticity. More preferably, it is to use thick foil or wormwood. In the case of mugwort selected as a natural substance, as well as the effect of suppressing the growth of various bacteria, it has an effect on eczema and itching of the skin such as bacterial dysentery, lacquer poison, grass poison, sweat band. In addition, bakbak activates antibacterial and digestive action, and when the leg is swollen, there is an effect to relieve swelling. They are usually used by diluting the solution eluted in a water bath or boiled water, and the preferred amount is 0.01 to 10.0% by weight based on the total weight of the composition, more preferably considering the investment price, color, and the formation of precipitates, more preferably 0.1-0.5 weight%.

The compositions of the present invention may further comprise ceramide thio peptidase which is produced from the species Serratia marcescens (Serratia sp.). The seratopeptidase described above has an anti-inflammatory effect, which helps to effectively treat inflammation in the foot.

Exogenous toxic substances invade in vivo and substances causing inflammation or pain are secreted. As a representative defense mechanism, anti-inflammatory enzymes remove the inflammatory substances or necrotic tissue.

As such anti-inflammatory enzymes, seraopeptidase, a proteolytic enzyme produced from Serratia sp., Is most commonly used as an anti-inflammatory agent. The seraopeptidase is a metal having a molecular weight of about 50 to 52 kD and containing Zn ²⁺ . As a metalloprotease, it is known as a protease whose optimum pH is weakly alkaline.

In addition to the above components, the composition of the present invention may further include conventional additives used in the composition for washing. Such examples include surfactants for generating cleansing, antibiotic, wetting, penetration, dispersion and foaming; Moisturizers for retaining moisture; Preservatives having antioxidant capacity and antiseptic ability against bacteria or fungi; Antioxidants that prevent oxidation of the skin; Antifungal agents that remove Candida fungi such as athlete's foot; Perfumery for removing foot odor and fragrance; Pigments that give a visual effect; Viscosity increasing agents to give the convenience and use of ointments and gels; And a moisture carrier for giving a moisturizing effect.

According to a preferred embodiment of the present invention, with respect to the total weight of the composition, 10 to 50% by weight of surfactant, 0.01 to 10% by weight of moisturizer, 0.01 to 0.5% by weight of preservative, 0.01 to 0.5% by weight of exfoliant, 0.01 to 0.5% of antioxidant 0.05 wt%, 5 to 16 wt% antifungal agent, 0.01 to 5 wt% perfume, 0.01 to 5 wt% pigment, 0.1 to 10 wt% viscosity increasing agent, 0.1 to 5 wt% water carrier, 0.01 to 1 keratinase Compositions containing 0.01% by weight to 10% by weight of natural substances cause synergism by the mixed composition, resulting in exfoliation, maintaining cleanliness and promoting metabolism, and have excellent exfoliation and antibacterial activity. It helped maintain the elasticity of the.

The foot cleaning composition of the present invention may add salt or sun salt to reinforce the function of athlete's foot prevention.

The foot cleaning composition of the present invention is not particularly limited in its formulation, and may be prepared, for example, in the form of a shampoo, cream, massage cream, lotion, gel, ointment or spray.

As shown in Figure 1 , the foot cleaning mixed composition of the present invention shows a better protein resolution than the conventional foot wash, and by adding keratin degrading enzyme, better protein resolution than the uncontrolled control ( Fig. 2 ) was confirmed.

As shown in the results of FIGS . 3 to 7, the mixed composition for washing feet, which further contains keratin degrading enzymes or natural substances derived from microorganisms, is a Gram-positive bacterium Bacillus subtilis , Staphylococcus aureus. ( Staphylococcus aureus ), or Enterococcus lactis shows a superior antimicrobial effect than the control streptomycin, and slightly lower or equivalent antimicrobial effect to the other control tetracycline.

However, against the Gram-negative bacterium Escherichia coli K12-594 and the pathogenic fungus Candida albicans, the material used as a control showed no antimicrobial effect, whereas the experimental group of the present invention showed excellent antimicrobial effect. Effect.

Therefore, the present invention is not only economical by using the microorganism-derived keratin degrading enzyme, but also shows an excellent antibacterial effect against the overall pathogens including gram positive bacteria, gram negative bacteria and mutant fungi, It can be suitably used for foot cleaning purposes.

Hereinafter, the present invention will be described in more detail with reference to Examples.

This embodiment is intended to illustrate the present invention in more detail, and the scope of the present invention is not limited to these examples. Those skilled in the art will be able to modify or supplement the present invention without departing from the scope and object of the invention.

Example 1 Preparation of Composition 1 for Foot Washing

As shown in Table 1 below, the composition of A was dissolved at 90 ° C., and then the compositions of A and B were mixed to complete dissolution at a temperature of 80 ° C. When the temperature of the reaction solution was lowered to 55 ° C, the composition of C was added, and after cooling to 30 ° C, the composition of D was added to prepare the composition 1 for foot washing.

Raw material name Content (% by weight) Remarks  Ammonium lauryl sulfate 20 A  Ammonium lauryl ethyl sulfate 10  methyl paraben 0.1  Coconut Acid diethanol amide 2.5  Glycerin One B  Propylene glycol 2  Salt 1.5  Thick One  EDTA 0.1  mentol One C  Incense (green tea) One  Pigment (Blue) slightly  Keratinase 0.05 D

Example 2 Preparation of Composition 2 for Foot Washing

Using the composition shown in Table 2 below, was carried out in the same manner as in Example 14, to prepare a composition for foot washing 2 having a weak viscosity, orange color and oily flavor of lilac.

Raw material name Content (weight,%) Remarks  Ammonium lauryl sulfate 15 A  Ammonium lauryl ethyl sulfate 20  Ethyl Sulfate 430 10  Coconut Acid diethanol amide 3  methyl paraben 0.1  Glycerin One B  Propylene glycol One  Salt 3  EDTA 0.1  Sodium sulfate 0.1  Alpha hydroxy acid 0.6  Urea 3  mentol One C  Incense (Lilac) slightly  Color (orange) slightly  Keratinase 0.05 D

Example 3 Preparation of Foot Washing Composition 3

As shown in Table 3 below, the compositions of A were dissolved at 80 ° C. and the compositions of B and C were dissolved at 80 ° C., respectively, and the compositions of A, B, and C were mixed when the temperature reached 65 ° C. When cooled to a temperature of 30 ° C., the composition of D was mixed to prepare a composition 3 for foot washing.

Raw material name Content (weight,%) Remarks  Ammonium lauryl sulfate 20 A  Coconut Acid diethanol amide 10  methyl paraben 5  Glycerin One B  Sodium sulfate 0.1  EDTA 0.1  allantoin 0.1  Salt 2  Citric acid 0.3 C  Thick One  Urea 0.5  Mentol One D  Incense (mint) One  Pigment (Blue) slightly Keratinase 0.05

Example 4 Preparation of Foot Washing Composition 4

In Table 4 below, the composition of A and the composition of B were dissolved at 80 ° C. and the composition of C was dissolved at 70 ° C., and then the compositions of A, B, and C were mixed. When the said mixture liquid cooled to 30 degreeC, the composition of D is mixed and it is carried out. A blue, viscous foot-washing composition 4 of suspension due to menthol was prepared.

Raw material name Content (weight,%) Remarks  Ammonium lauryl sulfate 30 A  Ammonium lauryl ethyl sulfate 5  Ethyl Sulfate 430 0.2  Glycerin One B  Propylene glycol 2  EDTA 0.1  Salt 3  Citric acid 0.3 C  Thick One  Urea 0.5  Mentol 1.5  allantoin 0.1  sodium benzoate 0.1  Keratinase 0.05 D

Example 5 Preparation of Foot Washing Composition 5

The compositions of A were dissolved at 85 ° C. and the compositions of B and C were dissolved at 80 ° C., respectively, and then the compositions of A, B, C, and D were mixed at a temperature of 65 ° C. The composition of E was mixed when cooled to 30 ° C., thereby preparing a foot washing composition 5 which was blue due to menthol and had a strong viscosity.

Raw material name Content (weight,%) Remarks  Ammonium lauryl sulfate 30 A  Ethyl Sulfate 430 15  Coconut Acid diethanol amide 5  methyl paraben 0.1  Propylene glycol 2 B  Glycerin One  Salt 5  EDTA 0.1  allantoin 0.1  Sodium sulfate 0.1  Sodium benzoate 0.1  Citric acid 0.3 C  Thick 2  Urea 0.5  Incense (Lilac) slightly D  mentol One  Keratinase 0.05 E

Example 6 Preparation of Foot Washing Composition 6

The composition of A was dissolved at 90 ° C. and then cooled to 80 ° C. to add the composition of B. The composition of C was dissolved at 90 ° C. and then cooled to 80 ° C. to add the composition of D. The mixture of A and B, the mixture of C and D, and the composition of E and F were mixed when cooled to 65 ° C., and the composition of G was added when the mixture was completely cooled to 30 ° C. Prepared.

Raw material name Content (weight,%) Remarks  Sodium Lauryl Sulfate 30 or more A  Ethyl Sulfate 430 More than 15  Lauric acid diethanol amide 5  methyl paraben 0.2  Urea 1.6 B  Propylene glycol 2 C  Glycerin One  Salt 2  EDTA 0.1  allantoin 0.5 D  Citric acid 0.3 E  Thick 2  Sodium sulfate 0.2  Sodium citrate 0.1  Incense (Lilac) slightly F  mentol One  Keratinase 0.05 G

Example 7 Preparation of Foot Washing Composition 7

The composition of C was mixed when the composition of A and the composition of B were each dissolved at 80 ° C. and cooled to 50 ° C. When the temperature of the liquid mixture of A, B, and C reached 30 ° C., the composition of D was added to give a turquoise, transparent liquid foot washing composition 7.

Raw material name Content (weight,%) Remarks  Sodium Lauryl Sulfate 35 A  Coconut Acid diethanol amide 5  methyl paraben 0.1  Propylene glycol 2 B  Salt 2  EDTA 0.1  allantoin 0.2  Thick 2  Sodium benzoate 0.1  Urea 2  mentol One C  Keratinase 0.05 D

Example 8 Preparation of Foot Washing Composition 8

The composition of A was dissolved at 85 ° C. and the composition of B was dissolved at 80 ° C. and mixed. When the mixture of A and B reached 65 ° C., C was mixed and the composition of D was mixed at a temperature of 55 ° C. The mixed solution was cooled to 30 ° C and the composition of E was added to prepare a composition 8 for foot washing.

Raw material name Content (weight,%) Remarks  Ammonium lauryl sulfate 35 A  Coconut Acid diethanol amide 5  methyl paraben 0.1  Propylene glycol 2 B  Salt 3  EDTA 0.1  allantoin 0.2  Thick 2  Sodium benzoate 0.2  Urea 2  Citric acid 0.3  Pigment (Blue) slightly C  menthol One D  Keratinase 0.05 E

Example 9 Preparation of Foot Washing Composition 9

The composition of A and the composition of B were dissolved at 80 ° C. and the composition of C was mixed when cooled to 45 ° C. When the mixture of A, B, and C was about 30 ° C., D was mixed to prepare a composition 9 for foot washing.

Raw material name Content (weight,%) Remarks  Sodium Lauryl Sulfate 40 A  Coconut Acid diethanol amide 5  methyl paraben 0.1  Propylene glycol 2 B  Salt 3  EDTA 0.1  allantoin 0.2  Thick 2  Sodium benzoate 0.1  Urea 2  menthol One C  Incense (pepamine or mint) 1.5  Keratinase 0.05 D

Example 10 Preparation of Foot Washing Composition 10

The composition of A and the composition of B were dissolved at 80 ° C. and the composition of C was mixed when cooled to 45 ° C. When the mixed solution of A, B, and C was about 30 ° C., D was mixed to prepare a composition 10 for foot washing.

Raw material name Content (weight,%) Remarks  Sodium Lauryl Sulfate 40 A  Coconut Acid diethanol amide 5  methyl paraben 0.1  Propylene glycol 2 B  Salt 3  EDTA 0.1  allantoin 0.2  Thick 2  Sodium benzoate 0.1  Urea 2  menthol One C  Incense (pepamine or mint) 1.5 Keratinase 0.05 D Cerathiopeptidase 0.01

Whether it is suitable as a use for the foot cleaning composition of the present invention prepared in the above embodiment was tested as follows.

Experimental Example 1 Protein Resolution Experiment

In order to determine the resolution of the protein for the foot washing composition of the present invention keratin-containing protein of the stratum corneum as follows.

1.5% of agar was mixed with 2% of skim milk and autoclaved at 121 ° C. for 15 minutes to prepare a protein activity measurement medium. The experimental group to which the control and the foot washing composition of the present invention was added to the prepared medium was prepared and reacted at 37 ° C. for 18 to 24 hours to measure protein resolution.

The control group is a foot cleanser of an external product (B; Body shop, BSK corporation, UK) having exfoliating ability, an emulsifying effect, a moisturizing effect, an antioxidant effect, and an exfoliating ability and a cleansing effect of a foreign product (C; green tea) Scrub, Elizabeth Arden, USA), the experimental group was used in the composition prepared in Example 9.

As shown in FIG. 1, protein resolution was not observed in the foot washes of the respective foreign acids as a control (B, C), whereas the composition of the present invention showed high protein resolution (A), and the keratin, which is the main component of the stratum corneum, was used. It was confirmed that the protein can be effectively used to effectively remove the exfoliation and the cleaning of the containing protein.

Experimental Example 2 Measurement of Synergy by Keratin Degrading Enzyme

In order to determine the synergism by keratinase (Synergism) was tested as follows.

The composition of Example 9 and the composition prepared without adding keratinase in Example 9 were tested for protein resolution in the same manner as in Experimental Example 1, and the results are shown in FIG. 2.

As a result, the control group without addition of keratin degrading enzyme was able to confirm the degradation of some proteins by the exfoliating agent, but the effect was insufficient (B). However, the composition prepared by adding keratin degrading enzyme showed a relatively high proteolytic ability (A).

From the above results, the keratinase was further supplied to the exfoliant to provide a synergistic effect on protein resolution.

Experimental Example 3 Antimicrobial Activity Test against Hospital Microorganisms

The antimicrobial activity test against the pathogenic microorganisms for the foot cleaning composition prepared in the above example was carried out as follows, and the results are shown in FIGS. 4 to 7. 4 to 7, A is the result obtained by treating the foot washing composition (5 μl) prepared in Example 9, B is the result obtained by treating streptomycin (Stretomycin, 50 μg / μl), C Is the result obtained by treatment with Tetracyclin (2 μg / μl).

<1-1> Antimicrobial test for Gram-positive bacteria

Gram-positive Bacillus subtilis (KCTC No. 3068), Staphylococcus aureus ( KCTC No. 1928), And Enterococcus lactis (KCTC No. 2012) were used, and were used by the Korea Collection for Type Culture (KCTC). Culture of the pathogen strain for the experiment was passaged in LB agar medium (1% bactopeptone, 0.5% yeast extract, 1% sodium chloride, 1.2% agar), and then taken a single colony (liquid colony), liquid at 37 ℃ for 24 hours Incubated. A portion of the culture was again incubated for 3 to 6 hours to maintain a mid-logarithmic state (OD 650.0.3).

After 6 ml of sterile LB agar medium was cooled to about 45 ° C., each strain was brought to a concentration of 10 ⁷ CFU / ml medium, mixed with LB medium, poured into a plate, and cooled sufficiently. 2 ㎡ holes were added to the completely hardened medium, and 2 μl of the control group and the experimental group were added thereto, followed by incubation at 37 ° C. for 18 to 24 hours to test the antibacterial ability.

As can be seen in Figures 3 and 4, the composition of the present invention is higher antibiotic than streptomycin used as a control for the Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus It showed an effect, but showed a low antimicrobial activity against tetracycline, another control.

On the other hand, the results for the gram-positive bacteria Enterococcus lactis ( Enterococcus lactis ), as shown in Figure 5, showed little activity in the control streptomycin and tetracycline, but the composition of Example 9 of the experimental group has a high antimicrobial activity Showed.

From the above results, the composition of the present invention is Gram-positive bacteria Bacillus subtilis (KCTC No. 3068), Staphylococcus aures (KCTC No. 1928), And it can be confirmed that it has an even antibiotic ability against Enterocococcus lactis (KCTC No. 2012).

<1-2> Antimicrobial test for Gram-negative bacteria

As Gram-negative bacteria, Escherichia coli K12-594 obtained from the Korean Collection for Type Culture (KCTC) was used in the same manner as in Step 1.

The control group used the same concentration of streptomycin (Streptomycin, Sigma. USA) and tetracycline (Tetracycline, Sigma. USA) used in step 1, the experimental group was the same foot cleaning composition prepared in Example 9 Used as a concentration.

From the results of FIG. 6, the control group showed weak antibiotic ability against the Gram-negative bacterium Escherichia coli K12-594, whereas the experimental group to which the composition of Example 9 was added showed a relatively high antibiotic ability.

<1-3> Antibacterial test for fungi

As a fungal bacterium, a fungal bacterium ( Candida albicans KCTC No. 7965) obtained from the Korean Collection for Type Culture (KCTC) was used.

Culture of the pathogen strain for the experiment was passaged in tomato dextrose agar medium (30% dice potato, 2% glucose, 1.2% agar) and then taken a single colony (single colony), at 30 ℃ Liquid culture for 24 hours. A portion of the culture solution was further liquid cultured for 3 to 6 hours to maintain a mid logarithm state (OD 650.0.3).

After 6 ml of sterile LB agar medium was cooled to about 45 ° C., each strain was brought to a concentration of 10 ⁷ CFU / ml medium, mixed with LB medium, poured into a plate, and cooled sufficiently. 2 ㎡ holes were added to the completely hardened medium, and 2 μl of the control group and the experimental group were added thereto, followed by incubation at 30 ° C. for 18 to 24 hours, to test the antibacterial ability.

The control group and the experimental group were selected the same as those used in step 1 and used at the same concentration.

As a result, while the control group Streptomycin and tetracycline did not observe the antibiotic ability against the fungi ( Candida albicans ), the composition of Example 9 of the experimental group showed a high antibiotic ability (FIG. 7). Therefore, the composition of the present invention can be usefully used as a foot cleanser, as described above, by showing an effect superior to conventionally used antibiotics for Gram-positive bacteria, Gram-negative bacteria, and fungi.

Experimental Example 4 Anti-inflammatory Effect Experiment

The anti-inflammatory effect was measured using the composition of Example 9 and the composition of Example 10.

Brown Schmidt's method [Braun, V. & Schmitz, G., Arch, Microbiol . 1980, 124: 55-61, titers of proteases of the compositions prepared in Examples 9 and 10 were measured. More specifically, 0.24 g of azo-casein (Sigma, USA) is dissolved in 10 ml of 50 mM Phosphate buffer (pH 7.5) to prepare a substrate solution, and 300 μl of substrate solution and 100 μl of cell culture solution. After mixing and reacting at 37 ° C. for 30 minutes, 300 μl of 10% trichloride acetate was added to the reaction solution and reacted at room temperature for 1 hour. The reaction solution was centrifuged at 7,000 rpm to separate the pellet and the supernatant, and 30 µl of 10% sodium hydroxide was added to 300 µl of the supernatant, and the absorbance was measured at 420 nm. The titer of the enzyme was defined as 1 unit when the absorbance value increased by 1.0. One unit of this method quantitatively measured the amount of azo casein, a protease-substrate substrate, eluted into azo and casein through digestion per minute at 37 ° C.

As a result of the experiment, it was observed that the composition of Example 10 had about 1.7 times improved proteolytic activity compared to the composition of Example 9. Therefore, it was confirmed that by further comprising a cerapiopeptase having an anti-inflammatory effect, it is effective in the treatment of inflammation.

As described above, the present invention includes a keratin degrading enzyme and a keratin remover derived from a microorganism, and has obtained a composition for cleaning feet, characterized in that it further comprises a ceratopeptidase or a natural substance. By additionally containing a keratin degrading enzyme or a natural substance selected from the group of natural plants, it causes a synergistic effect in degrading the protein containing keratin, the main component of the stratum corneum, enhances antimicrobial effect and metabolism, Exfoliation, cleanliness, and health, the composition of the present invention is Bacillus subtilis, Staphylococcus Aureus, It can be usefully used as a foot cleaning agent by showing excellent antibiotic effect over gram-positive bacteria such as enterococcus lactis, gram-negative bacteria of Escherichia coli, and fungi.

1 is a result of comparing the protein resolution of the foot cleaning composition of the present invention and the conventional foot cleaning agent,

Figure 2 is a result of comparing the protein resolution of the test group and keratin added with keratinase,

Figure 3 shows the antibiotic effect on the Gram-positive bacteria Bacillus subtilis ( Bacillus subtilis ),

Figure 4 shows the antibiotic effect on the Gram-positive bacteria Staphylococcus aureus ,

Figure 5 shows the antibiotic effect against Gram-positive bacteria Enterococcus lactis ,

Figure 6 shows the antibiotic effect on the Gram-negative bacteria Escherichia coli K12-594,

Figure 7 shows the antibiotic effect on the pathogenic fungus Candida albicans .

Claims (9)

One or more natural substances selected from the group consisting of humbac, mugwort, green tea, ogapi, ginseng, garlic and ginkgo biloba; One or more exfoliants selected from the group consisting of alpha hydroxy acids, beta hydroxy acids, urea, isoserine, bromelain, papain; And Antibacterial and antifungal foot washing composition comprising keratin degrading enzyme. The composition of claim 1, wherein the natural substance is thick or wormwood. The composition of claim 1, wherein the composition for foot cleansing may further include ceratiopeptidase. The method according to claim 1, wherein the foot cleaning composition is selected from the group consisting of surfactants, humectants, preservatives, antioxidants, antifungal agents, flavoring agents, pigments, viscosity increasing agents, water carriers, flavoring agents, and mixtures thereof. A composition for washing feet, further comprising a component. The foot cleansing composition according to claim 1, wherein the exfoliant is alpha hydroxy acid or urea. The composition of claim 1, wherein the keratinase is a liquid, a concentrated liquid concentrate, or a dry preparation. The composition of claim 1, wherein the keratinase is 0.01 to 1.00 wt% with respect to the total weight of the composition. The mixed composition for washing feet according to claim 1, wherein the natural substance is 0.1 to 0.5% by weight based on the total weight of the composition. The composition of claim 1, wherein the foot cleaning composition is in the form of a shampoo, cream, massage cream, lotion, gel, ointment or spray.