JP2507425B2 - Cosmetics - Google Patents

Description

[Detailed Description of the Invention] [Field of industrial application] The present invention relates to a cosmetic material exhibiting deodorant and antibacterial effects.

[Conventional technology]

There are cosmetics for the skin or in the mouth that contain an antibacterial substance for the purpose of deodorizing and antibacterial effects. For example, lotions and creams for acne, deodorant agents, mouthwashes, cleaning agents and the like are used therein, and various antibacterial substances are blended depending on the application and the form of the product. In addition, these antibacterial substances are often blended with cosmetics other than the above in order to preserve the cosmetics themselves.

Examples of the antibacterial substance include oxidol (hydrogen peroxide solution), reverse soap (cationic activator), chlorthymol, salicylic acid, zalol (phenyl salicylate),
Commonly used are phenolic acid (phenol), nitrofurazone, hinokitiol, hexachlorophene, boric acid, sodium borate and the like.

[Problems to be solved by the invention]

However, the above-mentioned antibacterial substances generally have a large or small degree of irritation, toxicity, and toxicity to the human body as well as deodorant and antibacterial properties.
It also has side effects such as allergenicity, which limits the types and amounts of antibacterial substances that can be mixed depending on the use, purpose, etc. when manufacturing cosmetics. At the same time, caution is required in actual use.

In view of the above circumstances, the present invention is a cosmetic containing a safe antibacterial substance excellent in biocompatibility because it has high deodorant properties, antibacterial properties, and does not have irritation, toxicity, allergenicity, etc. The purpose is to provide a fee.

[Means for solving problems]

In view of the above problems, the present inventors have paid attention to antibacterial substances existing in nature and investigated the relationship between the deodorant properties and antibacterial properties and biocompatibility of various compounds. As a result, it was found that chitosan obtained from chitin has excellent deodorant property, antibacterial property and biocompatibility. However, such a chitosan is a solid insoluble in water in a normal state, and it is difficult to apply it to the cosmetics in various forms such as a solution or an emulsion in the normal state. Therefore, as a result of further examination,
Completed this invention.

That is, this invention is characterized by a low-molecular weight chitosan water-soluble acid salt obtained by reacting an average molecular weight of 100,000 or less chitosan with 0.1% by weight or more of cosmetics. .

[Work]

 Hereinafter, the cosmetic material of the present invention will be described in detail.

The water-soluble acid salt of chitosan (hereinafter referred to as “chitosan salt”) used in the present invention is obtained by deacetylating chitin obtained from the shells of crustaceans such as shrimp and crab, mushrooms, bacterial cell walls, etc. It is obtained by reacting the obtained chitosan with an organic acid or an inorganic acid.

Chitosan generally has an average molecular weight of 200,000 or more and low solubility in acid, so that it tends to be unable to sufficiently react with acid as it is. Therefore, when reacting with acid, the molecular weight of chitosan was previously determined.
After reducing the molecular weight to 100,000 or less, it is reacted with an acid.

Examples of the method for reducing the molecular weight of chitosan include a chemical treatment method using an oxidizing agent, an enzyme treatment method using an enzyme such as papain, cellulase, and acid protease.

As the acid that reacts with chitosan as described above, either an inorganic acid or an organic acid may be used. Examples of inorganic acids include hydrochloric acid, and examples of organic acids include formic acid, lactic acid, acetic acid, citric acid, glycolic acid, malonic acid, ascorbic acid, adipic acid, oxalic acid, propionic acid, tartaric acid, succinic acid, L-glutamic acid, L-aspartic acid etc. are mentioned.

When sulfuric acid is used as the acid, the obtained chitosan salt is not water-soluble but water-insoluble, and a sufficient antibacterial action cannot be obtained. Therefore, the chitosan salt blended in the cosmetic of the present invention needs to be a water-soluble acid salt that is a reaction product with each of the above acids.

In order to react these chitosan and acid, it suffices to add an acid to the powder of chitosan, but these alone may cause a rapid reaction or a side reaction other than the predetermined reaction. Therefore, these reactions are usually performed in the presence of water. Further, this water is also necessary for converting the chitosan salt produced by the reaction into an aqueous solution having a predetermined concentration.

Examples of such a reaction method involving water include a method in which chitosan powder is dispersed in water and an acid is added while stirring the powder, but other methods may be used. Further, in reacting chitosan with an acid as described above, the degrading enzyme for lowering the molecular weight of chitosan may also be added to this reaction system, and the lowering of the molecular weight of chitosan and the dissolution may be performed at the same time. .

The amount of the acid to be added is not particularly limited in the present invention, but it is preferable to add an amount of at least the amount necessary to neutralize the free amino groups of chitosan, and the pH of the resulting aqueous chitosan salt solution is within the range of 3 to 6. It is more preferable to mix them in the following amounts.

The type of cosmetic amount is not particularly limited as long as the chitosan salt as described above is blended, and the present invention includes the above-mentioned lotion and cream for acne, deodorant agent, gargle,
It can be applied to all kinds of cosmetics including skin-cleaning agents, shaving agents, oral applications, etc.

Examples of these cosmetics include the followings, but needless to say, those other than those shown here may be used.

Animal and vegetable oil, animal and vegetable wax, mineral wax, hydrocarbon,
An oil-based cleansing cream or cleansing composition containing an oil-based component such as fatty acids, or a milk-like cleansing cream in which an emulsifier such as a nonionic surfactant, a cationic surfactant or an amphoteric surfactant is further added to the oil-based component. lotion,
Mucus lotion, emulsion (emulsifying lotion), two-layer lotion, creams, foundations, deodorant cream, jelly shampoo, cream shampoo, shaving cream, etc.

Aqueous solution or aqueous gel containing water-soluble saccharides, water-soluble polymer compounds, or alcohol-based lotions, deodorant lotions, liquid shampoos, shaving lotions, cleaning lotions based on alcohols, Gargle etc.

Powder, solid or paste soap, powder foundation, baby powder, antiperspirant powder, powder shampoo, toothpaste, etc.

The chitosan salt to be blended in the cosmetics as described above,
Depending on the form of the cosmetic, it may be blended as it is in the state of an aqueous solution, or may be blended in the form of powder after removing water.

Of the above-mentioned forms, the presence of water is not preferable in the oily or powdery form, so powdered chitosan salt is blended. Depending on the production method, it is possible to mix the chitosan salt as an aqueous solution in the form of oil or powder.

In other forms, the chitosan salt may be blended as an aqueous solution, but a powdered chitosan salt may be used. In that case, there is an advantage that the concentration of chitosan salt can be increased more than that of the aqueous solution. As a method for pulverizing the chitosan salt as described above, a usual drying method such as spray drying or freeze drying can be applied.

The amount of chitosan salt added is not particularly limited in the present invention, but the amount of chitosan salt added to the cosmetic is 0.1% by weight.
That is all. This is because if the amount of chitosan salt added is less than 0.1% by weight, sufficient antibacterial and deodorant effects may not be obtained.

The following tests were conducted to investigate the deodorant and antibacterial effects of these chitosan salts.

A trypticase soy broth medium (culture solution) containing 0.025 to 0.25% by weight of lactate of chitosan with an estimated molecular weight of 15,000 estimated from the intrinsic viscosity when 1% by weight of chitosan was dissolved in a 1% by weight acetic acid aqueous solution. Created. Next, in advance, trypticase soy broth medium 37
The following 5 strains, which are the source of odor and putrefaction, which had been cultivated at ℃ for 2 days, each contained 100 bacteria per ml.
The tryptocase soy broth medium containing the lactate of chitosan was inoculated to about 1000.

<< Strains Used >> Staphylococcus aureus Bacillus subtilis Bacillus megaterium Lactobacillus plantalum Lactobacillus sporogenes The above-mentioned bacteria and media are as follows.

・ Staphylococcus aureus: A typical Pseudomonas aeruginosa, also called Staphylococcus.

-Bacillus subtilis: Bacillus subtilis, aerobic or facultative anaerobic bacterium, which is an airborne bacterium.

・ Bacillus megaterium: A kind of heterotrophic bacteria, saprophytes,
Parasite. There are pathogenic bacteria or symbiotic bacteria.

・ Lactobacillus plantalum: Lactobacillus converts sugar into lactic acid. It often lives in the digestive tract of the animal, the oral cavity, etc.

・ Lactobacillus sporogenes: A bacterium that contributes to rancidity and emits a foul odor that causes spoilage.

Trypticase soy broth medium: formed by dissolving the following ingredients in purified water 1.

Polypeptone (casein peptone) 17 g Polypeptone (soybean peptone) 3 g Sodium chloride 5 g Dipotassium phosphate 2.5 g Glucose 2.5 g ・ Soybean casein digest agar medium (described later):
It is formed by dissolving the following components in purified water 1, and is used for bacteria inspection of medicines and cosmetics.

Polypeptone (casein peptone) 15 g Polypeptone (soybean peptone) 5 g Sodium chloride 5 g Agar 15 g The trypticase soy broth medium inoculated with the above strain was cultured at 30 ° C., and the growth conditions after 3 days and 7 days were determined. The determination was made by smearing the above medium solution on the soybean casein digest agar medium, and determining the degree of agglutination of the strain used and the agglutination of the medium protein by chitosan. Further, a trypticase isobroth medium containing no chitosan was similarly cultured, and the result was used as a control.
The results are shown in Table 1. The judgment symbols in Table 1 are as follows.

From the results shown in Table 1, it was found that the one to which the chitosan salt was added can relatively suppress the growth of these strains, as compared with the one to which the chitosan salt was not added. It was also found that the one to which chitosan salt was added did not generate an odor and had a high deodorizing property.

〔Example〕

Next, examples of the present invention will be described together with comparative examples.

(Examples 1 to 6 and Comparative Example 1) Of the components shown below, four components from glyceryl monostearate to cetanol were mixed and dissolved at 80 ° C,
This was added to purified water which was also heated to 80 ° C., emulsified with a homogenizer, and cooled to room temperature to obtain an emulsion. A lactate aqueous solution of chitosan (pH 5.0, 5% by weight aqueous solution) was added so that the pure content of chitosan relative to the total amount of this emulsion was the value shown in Table 2 to prepare a sample. The chitosan lactate used had an estimated molecular weight of 15,000 from the intrinsic viscosity.

<Ingredients> Glycerin monostearate 1.5% by weight Liquid paraffin 1.0% by weight Polyoxyethylene cetostearyl alcohol 1.0% by weight Cetanol 1.0% by weight Purified water 95.5 % by weight Total 100.0% by weight The obtained sample was heated to 80 ° C. After sterilization, the following 3 strains, which are the source of odor and spoilage, were cultivated in trypticase soy broth medium at 37 ℃ for 2 days in advance. Was inoculated so that

《Strains used》 Staphylococcus aureus Bacillus subtilis Escherichia coli In addition, of the above-mentioned bacteria, the former two are as described above. And is a representative bacterium of the group of intestinal bacteria. The presence of this bacterium serves as an index of the degree of contamination and indicates the risk of contamination by pathogenic bacteria. The presence of this type of bacteria degrades skin secretions and causes odor, so it was tested here as a representative of Gram-negative contaminants.

These samples were cultured at 30 ° C., and the growth status after 7 days and 30 days was determined. The determination was made by plating the above medium solution on a soybean casein digest agar medium, determining the degree of agglutination of the strain used, and the agglutination of the medium protein by chitosan. Further, the alteration of the emulsion was judged by the change in its appearance, and the effect was judged by combining both. The results are shown in Table 2. The judgment symbols in Table 2 are as follows.

From the results of Table 2, Examples 1 to 1 which are cosmetics of the present invention
It was found that each of 6 has a higher antibacterial effect than Comparative Example 1, and has both a sterilizing action on the skin and an antiseptic action itself.

(Examples 7 to 10) A deodorant lotion composed of the following components was prepared to prepare a sample solution.

Using a sample solution adjusted at <ingredients> Propylene glycol 3.0 wt% chitosan salt (10 wt% aqueous solution) 0.25 to 2.5 wt% ethanol 65.0 wt% purified water Balance Total 100.0% by weight or more of Examples 7 to 10, The following tests were conducted.

(Test) A control solution prepared by exfoliating about 20 cm square gauze gauze into one of the sample solutions of Examples 7 to 10 and the other prepared by removing chitosan salt stains from the above component mixture. Was sprayed to contain about 1 cc of each. Gauze sprayed with the sample solution was placed under the right axilla of 10 subjects, and 10 gauze sprayed with the control solution was applied.
Under the left axilla of each of the subjects, with paper tape,
The person evaluated the difference in body odor transferred to gauze after about 12 hours. The results are shown in Table 3.

Further, after the test, when the axilla under contact with gauze was observed, no allergic symptoms due to irritation were observed at any chitosan concentration.

From the results of Table 3, Examples 7 to 7 which are cosmetics of the present invention are shown.
According to 10, including those that recognize a slight difference in effect,
It was found that a clear deodorizing effect could be obtained.

〔The invention's effect〕

The cosmetic of the present invention is as described above, wherein chitosan is added with 0.1% by weight or more of a low molecular weight chitosan water-soluble acid salt obtained by reacting chitosan having an average molecular weight of 100,000 or less with an acid. Therefore, it is highly safe and has excellent biocompatibility because it has high deodorant and antibacterial properties and does not have irritation, toxicity and allergenicity.

 ─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Shizuo Enokida 4-3-70 Nishihara-cho, Tanashi City (56) References JP-A-49-19040 (JP, A) JP-A-59-139310 (JP, A) JP-A-60-203602 (JP, A) JP-A-62-221615 (JP, A) JP-A-58-164601 (JP, A) JP-A-59-152312 (JP, A) JP-A-61-254517 (JP, A) JP-A-63-14714 (JP, A) JP-A-63-10715 (JP, A)

Claims (1)

(57) [Claims] 1. A cosmetic composition comprising 0.1% by weight or more of a low molecular weight chitosan water-soluble acid salt obtained by reacting chitosan having an average molecular weight of 100,000 or less with an acid.