JPS6318463B2 - - Google Patents
Info
- Publication number
- JPS6318463B2 JPS6318463B2 JP56111296A JP11129681A JPS6318463B2 JP S6318463 B2 JPS6318463 B2 JP S6318463B2 JP 56111296 A JP56111296 A JP 56111296A JP 11129681 A JP11129681 A JP 11129681A JP S6318463 B2 JPS6318463 B2 JP S6318463B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- medium
- phospholipid
- ethanolamine
- phospholipids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000002609 medium Substances 0.000 claims description 41
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 claims description 36
- 210000004027 cell Anatomy 0.000 claims description 19
- 150000003904 phospholipids Chemical class 0.000 claims description 18
- 210000004102 animal cell Anatomy 0.000 claims description 13
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 12
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 210000004408 hybridoma Anatomy 0.000 claims description 9
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 7
- 239000008347 soybean phospholipid Substances 0.000 claims description 7
- 102000004877 Insulin Human genes 0.000 claims description 6
- 108090001061 Insulin Proteins 0.000 claims description 6
- 239000006143 cell culture medium Substances 0.000 claims description 6
- 239000008344 egg yolk phospholipid Substances 0.000 claims description 6
- 229940068998 egg yolk phospholipid Drugs 0.000 claims description 6
- 229940125396 insulin Drugs 0.000 claims description 6
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 5
- 239000013543 active substance Substances 0.000 claims description 5
- 230000004927 fusion Effects 0.000 claims description 5
- 210000004754 hybrid cell Anatomy 0.000 claims description 5
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 5
- ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 claims description 3
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 claims description 3
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 3
- JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 claims description 3
- 210000002950 fibroblast Anatomy 0.000 claims description 3
- 229940067606 lecithin Drugs 0.000 claims description 3
- 235000010445 lecithin Nutrition 0.000 claims description 3
- 239000000787 lecithin Substances 0.000 claims description 3
- 210000000265 leukocyte Anatomy 0.000 claims description 3
- 150000008104 phosphatidylethanolamines Chemical class 0.000 claims description 3
- 150000003905 phosphatidylinositols Chemical class 0.000 claims description 3
- 108010051696 Growth Hormone Proteins 0.000 claims description 2
- 102000018997 Growth Hormone Human genes 0.000 claims description 2
- 102000014150 Interferons Human genes 0.000 claims description 2
- 108010050904 Interferons Proteins 0.000 claims description 2
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 2
- 239000000122 growth hormone Substances 0.000 claims description 2
- 229940079322 interferon Drugs 0.000 claims description 2
- 230000004663 cell proliferation Effects 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 210000002966 serum Anatomy 0.000 description 9
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 8
- 102000004338 Transferrin Human genes 0.000 description 8
- 108090000901 Transferrin Proteins 0.000 description 8
- 238000012258 culturing Methods 0.000 description 8
- 229910052711 selenium Inorganic materials 0.000 description 8
- 239000011669 selenium Substances 0.000 description 8
- 239000012581 transferrin Substances 0.000 description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 239000007640 basal medium Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000012980 RPMI-1640 medium Substances 0.000 description 5
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 4
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 101710186630 Insulin-1 Proteins 0.000 description 4
- 102000015439 Phospholipases Human genes 0.000 description 4
- 108010064785 Phospholipases Proteins 0.000 description 4
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- MMKZAICYSVWBPW-UHFFFAOYSA-N 2-[[2-(difluoromethoxy)phenyl]methylamino]-1-[3-(trifluoromethyl)phenyl]ethanol Chemical compound C=1C=CC(C(F)(F)F)=CC=1C(O)CNCC1=CC=CC=C1OC(F)F MMKZAICYSVWBPW-UHFFFAOYSA-N 0.000 description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 235000013345 egg yolk Nutrition 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 229940104230 thymidine Drugs 0.000 description 2
- -1 MCB4 Proteins 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- 102100033458 26S proteasome non-ATPase regulatory subunit 4 Human genes 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101150001079 PSMD4 gene Proteins 0.000 description 1
- 101150006293 Rpn10 gene Proteins 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000012007 large scale cell culture Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56111296A JPS5813389A (ja) | 1981-07-16 | 1981-07-16 | 細胞培養用培地 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56111296A JPS5813389A (ja) | 1981-07-16 | 1981-07-16 | 細胞培養用培地 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5813389A JPS5813389A (ja) | 1983-01-25 |
| JPS6318463B2 true JPS6318463B2 (enrdf_load_stackoverflow) | 1988-04-19 |
Family
ID=14557618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56111296A Granted JPS5813389A (ja) | 1981-07-16 | 1981-07-16 | 細胞培養用培地 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5813389A (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0948567A (ja) * | 1995-08-04 | 1997-02-18 | Bunso Rin | エレベータのカゴに付随する非常はしご装置 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2525022B2 (ja) * | 1986-01-03 | 1996-08-14 | ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド | ▲ＶＩＩＩ▼：ｃ因子型タンパク質の改良生産方法 |
| US5250421A (en) * | 1986-01-03 | 1993-10-05 | Genetics Institute, Inc. | Method for producing factor VIII:C-type proteins |
| US5198349A (en) * | 1986-01-03 | 1993-03-30 | Genetics Institute, Inc. | Method for producing factor VIII:C and analogs |
| CA1331157C (en) * | 1987-04-06 | 1994-08-02 | Randal J. Kaufman | Method for producing factor viii:c-type proteins |
| JPH0793880B2 (ja) * | 1988-02-29 | 1995-10-11 | 森永製菓株式会社 | モノクローナル抗体生産増強法 |
| WO2024185843A1 (ja) * | 2023-03-09 | 2024-09-12 | 味の素株式会社 | 動物細胞の培養方法 |
-
1981
- 1981-07-16 JP JP56111296A patent/JPS5813389A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0948567A (ja) * | 1995-08-04 | 1997-02-18 | Bunso Rin | エレベータのカゴに付随する非常はしご装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5813389A (ja) | 1983-01-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fugman et al. | In vitro establishment and characterization of a human megakaryoblastic cell line | |
| EP0066284B1 (en) | Culture medium and its use | |
| US5945337A (en) | Method for culturing CD34+ cells in a serum-free medium | |
| JPH02503744A (ja) | 第ＶＩＩＩ：ｃ因子型タンパク質の改良生産方法 | |
| US4288546A (en) | Process for the large scale production of pituitary hormones by serial secondary suspension culture | |
| JPS6318463B2 (enrdf_load_stackoverflow) | ||
| JPS585671B2 (ja) | ヒト卵胞刺激ホルモンの製造方法 | |
| JP2006506435A (ja) | ヒト血清アルブミンの結晶化の方法 | |
| EP0248656B1 (en) | Composition for cell cultivation and use thereof | |
| GB2083826A (en) | Process for the production of human insulin | |
| Murakami et al. | Stirred culture of myeloma MPC-11 cells in serum-free medium | |
| Teresi et al. | Further study of boron in the nutrition of the rat | |
| CA1279593C (en) | Cholesterol-rich fraction, process therefor and use thereof in cell culture | |
| JPH0728732B2 (ja) | 動物細胞増殖促進剤及び無血清培地 | |
| JPH0432078B2 (enrdf_load_stackoverflow) | ||
| Ogawa et al. | Isozyme patterns of branched-chain amino acid transaminase in cultured rat hepatocytes | |
| SU1830080A3 (ru) | Бессыворотомная питательная среда для культуры ткани | |
| JPS633787A (ja) | 改良された融合生成物 | |
| JPS633792A (ja) | 改良された融合方法 | |
| Lernhardt | Fatty acid requirement of B lymphocytes activated in vitro | |
| CN111808825B (zh) | 一种用于筛选杂交瘤细胞的选择培养基及应用 | |
| JPS5825439B2 (ja) | ヒト副甲状腺ホルモンの製造方法 | |
| JPS6251983A (ja) | ヒト／ヒト・ハイブリド−マ培養用無血清培地 | |
| US5602025A (en) | Non-tumorigenic cell lines for expression of genes | |
| JPS63141584A (ja) | 細胞培養用培地 |