JPS62192632A - 顕微鏡測光器 - Google Patents
顕微鏡測光器Info
- Publication number
- JPS62192632A JPS62192632A JP62031660A JP3166087A JPS62192632A JP S62192632 A JPS62192632 A JP S62192632A JP 62031660 A JP62031660 A JP 62031660A JP 3166087 A JP3166087 A JP 3166087A JP S62192632 A JPS62192632 A JP S62192632A
- Authority
- JP
- Japan
- Prior art keywords
- photometer
- microscope
- chopper wheel
- filters
- modulator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005286 illumination Methods 0.000 claims description 19
- 238000001514 detection method Methods 0.000 claims description 12
- 230000003287 optical effect Effects 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 230000004907 flux Effects 0.000 claims description 2
- 238000003384 imaging method Methods 0.000 claims 1
- 230000005284 excitation Effects 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 101001080808 Homo sapiens PH and SEC7 domain-containing protein 2 Proteins 0.000 description 4
- 102100027455 PH and SEC7 domain-containing protein 2 Human genes 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000003157 biological pigment Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- YFHXZQPUBCBNIP-UHFFFAOYSA-N fura-2 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=3OC(=CC=3C=2)C=2OC(=CN=2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1 YFHXZQPUBCBNIP-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 101001080825 Homo sapiens PH and SEC7 domain-containing protein 1 Proteins 0.000 description 1
- 102100027472 PH and SEC7 domain-containing protein 1 Human genes 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0096—Microscopes with photometer devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/42—Photometry, e.g. photographic exposure meter using electric radiation detectors
- G01J2001/4242—Modulated light, e.g. for synchronizing source and detector circuit
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N2021/1738—Optionally different kinds of measurements; Method being valid for different kinds of measurement
- G01N2021/174—Optionally different kinds of measurements; Method being valid for different kinds of measurement either absorption-reflection or emission-fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
Landscapes
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Microscoopes, Condenser (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19863604815 DE3604815A1 (de) | 1986-02-15 | 1986-02-15 | Mikroskopphotometer |
| DE3604815.1 | 1986-02-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62192632A true JPS62192632A (ja) | 1987-08-24 |
Family
ID=6294162
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62031660A Pending JPS62192632A (ja) | 1986-02-15 | 1987-02-16 | 顕微鏡測光器 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS62192632A (enrdf_load_stackoverflow) |
| DE (1) | DE3604815A1 (enrdf_load_stackoverflow) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02120056U (enrdf_load_stackoverflow) * | 1989-03-15 | 1990-09-27 | ||
| JPH0372238A (ja) * | 1989-08-12 | 1991-03-27 | Res Dev Corp Of Japan | 高感度顕微多波長分光装置 |
| JP2003501615A (ja) * | 1998-11-04 | 2003-01-14 | ザ リサーチ ファウンデーション オブ ステイト ユニヴァーシティ オブ ニューヨーク | 連続的な量子タイミングコードを有するサンプル分析 |
| JP2004132760A (ja) * | 2002-10-09 | 2004-04-30 | Shimadzu Corp | 複数の蛍光物質を含む試料の分析方法及び装置 |
| JP2011107160A (ja) * | 2007-06-21 | 2011-06-02 | Gen-Probe Inc | マルチチャネル光学測定機器 |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3915421C2 (de) * | 1989-05-11 | 1995-03-02 | Bayer Ag | Vorrichtung zur Messung der Fluoreszenzanregung biologischer Zellen bei zwei verschiedenen Wellenlängen |
| DE4115401C2 (de) * | 1991-05-10 | 1994-04-14 | Rainer Dr Uhl | Fluoreszenz-Meßvorrichtung zum Bestimmen der Ionenkonzentration eines Untersuchungsobjekts, das mit einem Fluoreszenzfarbstoff eingefärbt ist, dessen Anregungsmaximum sich in Abhängigkeit von der zu bestimmenden Ionenkonzentration ändert |
| DE4228366C2 (de) * | 1992-08-26 | 1995-05-24 | Rainer Dr Uhl | Fluoreszenz-Meßvorrichtung |
| DE4338531A1 (de) * | 1993-11-11 | 1995-05-18 | Leica Lasertechnik | Vorrichtung zur Mehrfarbbeleuchtung von Präparaten |
| US5672515A (en) * | 1995-09-12 | 1997-09-30 | Optical Sensors Incorporated | Simultaneous dual excitation/single emission fluorescent sensing method for PH and pCO2 |
| RU2182328C2 (ru) * | 2000-02-17 | 2002-05-10 | Институт молекулярной биологии им. В.А. Энгельгардта РАН | Флуоресцентный микроскоп |
| DE10231667A1 (de) | 2002-07-12 | 2004-01-22 | Olympus Biosystems Gmbh | Beleuchtungsvorrichtung und optische Objektuntersuchungseinrichtung |
| DE10362401B3 (de) | 2002-08-28 | 2022-03-10 | Carl Zeiss Meditec Ag | Mikroskopiesystem und Mikroskopieverfahren |
| GB0525072D0 (en) | 2005-12-09 | 2006-01-18 | Enigma Diagnostics Ltd | Fluorescence-based detection methods and apparatus |
| CN203719767U (zh) * | 2012-06-11 | 2014-07-16 | 保生国际生医股份有限公司 | 生化检测系统及其光源模块 |
| CN111398230A (zh) * | 2019-03-27 | 2020-07-10 | 上海交通大学 | 一种时间门控荧光成像系统 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49106382A (enrdf_load_stackoverflow) * | 1973-02-09 | 1974-10-08 | ||
| JPS5340541A (en) * | 1976-09-25 | 1978-04-13 | Olympus Optical Co Ltd | Quantitative detecting microscope |
| JPS53149088A (en) * | 1977-06-01 | 1978-12-26 | Hitachi Ltd | Optical absorption measuring system |
| JPS56501174A (enrdf_load_stackoverflow) * | 1979-08-23 | 1981-08-20 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5419263B2 (enrdf_load_stackoverflow) * | 1973-10-17 | 1979-07-13 | ||
| DE2614181C3 (de) * | 1976-04-02 | 1981-08-20 | Leybold-Heraeus Gmbh, 5000 Koeln | Verfahren zur Messung des optischen Absorptionsvermögens von Proben und Vorrichtung zur Durchführung des Verfahrens |
| US4100416A (en) * | 1977-03-02 | 1978-07-11 | Block Engineering, Inc. | Serum fluorescence suppression |
-
1986
- 1986-02-15 DE DE19863604815 patent/DE3604815A1/de active Granted
-
1987
- 1987-02-16 JP JP62031660A patent/JPS62192632A/ja active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49106382A (enrdf_load_stackoverflow) * | 1973-02-09 | 1974-10-08 | ||
| JPS5340541A (en) * | 1976-09-25 | 1978-04-13 | Olympus Optical Co Ltd | Quantitative detecting microscope |
| JPS53149088A (en) * | 1977-06-01 | 1978-12-26 | Hitachi Ltd | Optical absorption measuring system |
| JPS56501174A (enrdf_load_stackoverflow) * | 1979-08-23 | 1981-08-20 |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02120056U (enrdf_load_stackoverflow) * | 1989-03-15 | 1990-09-27 | ||
| JPH0372238A (ja) * | 1989-08-12 | 1991-03-27 | Res Dev Corp Of Japan | 高感度顕微多波長分光装置 |
| JP2003501615A (ja) * | 1998-11-04 | 2003-01-14 | ザ リサーチ ファウンデーション オブ ステイト ユニヴァーシティ オブ ニューヨーク | 連続的な量子タイミングコードを有するサンプル分析 |
| JP2004132760A (ja) * | 2002-10-09 | 2004-04-30 | Shimadzu Corp | 複数の蛍光物質を含む試料の分析方法及び装置 |
| JP2011107160A (ja) * | 2007-06-21 | 2011-06-02 | Gen-Probe Inc | マルチチャネル光学測定機器 |
| US9458451B2 (en) | 2007-06-21 | 2016-10-04 | Gen-Probe Incorporated | Multi-channel optical measurement instrument |
| US10086342B2 (en) | 2007-06-21 | 2018-10-02 | Gen-Probe Incorporated | Multi-channel optical measurement instrument |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3604815A1 (de) | 1987-08-20 |
| DE3604815C2 (enrdf_load_stackoverflow) | 1988-12-08 |
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