JPS61207340A - コラーゲン基質の細胞増殖性向上処理方法 - Google Patents
コラーゲン基質の細胞増殖性向上処理方法Info
- Publication number
- JPS61207340A JPS61207340A JP60048350A JP4835085A JPS61207340A JP S61207340 A JPS61207340 A JP S61207340A JP 60048350 A JP60048350 A JP 60048350A JP 4835085 A JP4835085 A JP 4835085A JP S61207340 A JPS61207340 A JP S61207340A
- Authority
- JP
- Japan
- Prior art keywords
- collagen
- treatment
- cells
- collagen matrix
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010035532 Collagen Proteins 0.000 title claims abstract description 61
- 102000008186 Collagen Human genes 0.000 title claims abstract description 61
- 229920001436 collagen Polymers 0.000 title claims abstract description 61
- 238000011282 treatment Methods 0.000 title claims abstract description 22
- 239000000758 substrate Substances 0.000 title abstract description 9
- 230000004663 cell proliferation Effects 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 14
- 210000000056 organ Anatomy 0.000 claims abstract description 13
- 238000012986 modification Methods 0.000 claims abstract description 11
- 230000004048 modification Effects 0.000 claims abstract description 11
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000004113 cell culture Methods 0.000 claims abstract description 7
- 239000005057 Hexamethylene diisocyanate Substances 0.000 claims abstract description 3
- 230000005251 gamma ray Effects 0.000 claims abstract description 3
- RRAMGCGOFNQTLD-UHFFFAOYSA-N hexamethylene diisocyanate Chemical compound O=C=NCCCCCCN=C=O RRAMGCGOFNQTLD-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000011159 matrix material Substances 0.000 claims description 25
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical group O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 5
- 150000001299 aldehydes Chemical class 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 108010045569 atelocollagen Proteins 0.000 abstract description 14
- 102000057297 Pepsin A Human genes 0.000 abstract description 4
- 108090000284 Pepsin A Proteins 0.000 abstract description 4
- 230000001678 irradiating effect Effects 0.000 abstract description 4
- 229940111202 pepsin Drugs 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000005855 radiation Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 21
- 239000000243 solution Substances 0.000 description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000007789 gas Substances 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 239000010409 thin film Substances 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 210000001339 epidermal cell Anatomy 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 210000003491 skin Anatomy 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000004698 Polyethylene Substances 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 230000002070 germicidal effect Effects 0.000 description 3
- 210000003494 hepatocyte Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- -1 polyethylene Polymers 0.000 description 3
- 229920000573 polyethylene Polymers 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241001529936 Murinae Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- NBBJYMSMWIIQGU-UHFFFAOYSA-N Propionic aldehyde Chemical compound CCC=O NBBJYMSMWIIQGU-UHFFFAOYSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 210000004207 dermis Anatomy 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- LEQAOMBKQFMDFZ-UHFFFAOYSA-N glyoxal Chemical compound O=CC=O LEQAOMBKQFMDFZ-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 241000257465 Echinoidea Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- IKHGUXGNUITLKF-XPULMUKRSA-N acetaldehyde Chemical compound [14CH]([14CH3])=O IKHGUXGNUITLKF-XPULMUKRSA-N 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- ZTQSAGDEMFDKMZ-UHFFFAOYSA-N butyric aldehyde Natural products CCCC=O ZTQSAGDEMFDKMZ-UHFFFAOYSA-N 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940015043 glyoxal Drugs 0.000 description 1
- 125000004836 hexamethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[*:1] 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000003863 physical function Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000001626 skin fibroblast Anatomy 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60048350A JPS61207340A (ja) | 1985-03-13 | 1985-03-13 | コラーゲン基質の細胞増殖性向上処理方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60048350A JPS61207340A (ja) | 1985-03-13 | 1985-03-13 | コラーゲン基質の細胞増殖性向上処理方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61207340A true JPS61207340A (ja) | 1986-09-13 |
| JPH0586185B2 JPH0586185B2 (enrdf_load_stackoverflow) | 1993-12-10 |
Family
ID=12800924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60048350A Granted JPS61207340A (ja) | 1985-03-13 | 1985-03-13 | コラーゲン基質の細胞増殖性向上処理方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61207340A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62265978A (ja) * | 1986-05-12 | 1987-11-18 | Yasuo Moriya | 表皮細胞培養器 |
| JP2005000314A (ja) * | 2003-06-10 | 2005-01-06 | Gunze Ltd | コラーゲンスポンジの製造方法、人工皮膚の製造方法、人工皮膚及び細胞組織培養基材 |
| JP2020080722A (ja) * | 2018-11-26 | 2020-06-04 | 大日本印刷株式会社 | 細胞の収縮を抑制できる細胞取扱容器、及び細胞構造体の作製方法 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5276416A (en) * | 1975-12-22 | 1977-06-27 | Nikkiso Co Ltd | Preparation of medical material with antiaggulutinating activity |
| JPS543779A (en) * | 1977-06-10 | 1979-01-12 | Akebono Brake Ind Co Ltd | Device for contrlling brake |
| JPS5559A (en) * | 1978-06-14 | 1980-01-05 | Nippi:Kk | Preparation of immobilized bio-active substance |
| JPS573634A (en) * | 1980-06-06 | 1982-01-09 | Koken Kk | Implant material for live body and method |
| JPS5946594A (ja) * | 1982-09-09 | 1984-03-15 | 株式会社東芝 | 原子炉炉心冷却系の流量検定装置 |
| JPS6176161A (ja) * | 1984-09-22 | 1986-04-18 | 新田ゼラチン株式会社 | コラ−ゲンの滅菌法 |
-
1985
- 1985-03-13 JP JP60048350A patent/JPS61207340A/ja active Granted
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5276416A (en) * | 1975-12-22 | 1977-06-27 | Nikkiso Co Ltd | Preparation of medical material with antiaggulutinating activity |
| JPS543779A (en) * | 1977-06-10 | 1979-01-12 | Akebono Brake Ind Co Ltd | Device for contrlling brake |
| JPS5559A (en) * | 1978-06-14 | 1980-01-05 | Nippi:Kk | Preparation of immobilized bio-active substance |
| JPS573634A (en) * | 1980-06-06 | 1982-01-09 | Koken Kk | Implant material for live body and method |
| JPS5946594A (ja) * | 1982-09-09 | 1984-03-15 | 株式会社東芝 | 原子炉炉心冷却系の流量検定装置 |
| JPS6176161A (ja) * | 1984-09-22 | 1986-04-18 | 新田ゼラチン株式会社 | コラ−ゲンの滅菌法 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62265978A (ja) * | 1986-05-12 | 1987-11-18 | Yasuo Moriya | 表皮細胞培養器 |
| JP2005000314A (ja) * | 2003-06-10 | 2005-01-06 | Gunze Ltd | コラーゲンスポンジの製造方法、人工皮膚の製造方法、人工皮膚及び細胞組織培養基材 |
| JP2020080722A (ja) * | 2018-11-26 | 2020-06-04 | 大日本印刷株式会社 | 細胞の収縮を抑制できる細胞取扱容器、及び細胞構造体の作製方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0586185B2 (enrdf_load_stackoverflow) | 1993-12-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |