JPS5910190B2 - 新規な乳酸オキシダ−ゼの製造法 - Google Patents
新規な乳酸オキシダ−ゼの製造法Info
- Publication number
- JPS5910190B2 JPS5910190B2 JP53073619A JP7361978A JPS5910190B2 JP S5910190 B2 JPS5910190 B2 JP S5910190B2 JP 53073619 A JP53073619 A JP 53073619A JP 7361978 A JP7361978 A JP 7361978A JP S5910190 B2 JPS5910190 B2 JP S5910190B2
- Authority
- JP
- Japan
- Prior art keywords
- lactic acid
- lactate oxidase
- around
- acid
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108010073450 Lactate 2-monooxygenase Proteins 0.000 title claims description 67
- 238000004519 manufacturing process Methods 0.000 title claims description 11
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N Lactic Acid Natural products CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 94
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 61
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 claims description 57
- 102000004190 Enzymes Human genes 0.000 claims description 51
- 108090000790 Enzymes Proteins 0.000 claims description 51
- 239000004310 lactic acid Substances 0.000 claims description 46
- 235000014655 lactic acid Nutrition 0.000 claims description 44
- 238000006243 chemical reaction Methods 0.000 claims description 35
- 241000894006 Bacteria Species 0.000 claims description 27
- 239000000758 substrate Substances 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 23
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 claims description 20
- 238000005259 measurement Methods 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- 239000001301 oxygen Substances 0.000 claims description 17
- 229910052760 oxygen Inorganic materials 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 16
- 238000004458 analytical method Methods 0.000 claims description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 15
- 238000001962 electrophoresis Methods 0.000 claims description 13
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 12
- 229940076788 pyruvate Drugs 0.000 claims description 12
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 claims description 11
- 229940107700 pyruvic acid Drugs 0.000 claims description 10
- 102000003992 Peroxidases Human genes 0.000 claims description 9
- 241000194017 Streptococcus Species 0.000 claims description 9
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 9
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- 230000009471 action Effects 0.000 claims description 7
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 claims description 6
- 230000002255 enzymatic effect Effects 0.000 claims description 6
- 238000006911 enzymatic reaction Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000003086 colorant Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 241000193798 Aerococcus Species 0.000 claims description 4
- 241000194022 Streptococcus sp. Species 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 229940088598 enzyme Drugs 0.000 description 48
- 229960000448 lactic acid Drugs 0.000 description 45
- 239000000243 solution Substances 0.000 description 29
- 230000000694 effects Effects 0.000 description 20
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 15
- 210000002966 serum Anatomy 0.000 description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000000872 buffer Substances 0.000 description 12
- 238000011088 calibration curve Methods 0.000 description 11
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- 239000008363 phosphate buffer Substances 0.000 description 8
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 7
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- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
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- 108010014251 Muramidase Proteins 0.000 description 5
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 5
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 5
- 238000000502 dialysis Methods 0.000 description 5
- 229960000274 lysozyme Drugs 0.000 description 5
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- 235000010335 lysozyme Nutrition 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- -1 peptone Chemical class 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000000654 additive Substances 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 150000001768 cations Chemical class 0.000 description 4
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- 238000012360 testing method Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 241000194031 Enterococcus faecium Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000604136 Pediococcus sp. Species 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 230000010534 mechanism of action Effects 0.000 description 3
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- 229920002401 polyacrylamide Polymers 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 2
- 241000193792 Aerococcus viridans Species 0.000 description 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- 241001478240 Coccus Species 0.000 description 2
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 108010042687 Pyruvate Oxidase Proteins 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
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- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 2
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- 229940041514 candida albicans extract Drugs 0.000 description 2
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- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 description 2
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- 238000010828 elution Methods 0.000 description 2
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- FVTCRASFADXXNN-SCRDCRAPSA-N flavin mononucleotide Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-SCRDCRAPSA-N 0.000 description 2
- 239000011768 flavin mononucleotide Substances 0.000 description 2
- 229940013640 flavin mononucleotide Drugs 0.000 description 2
- FVTCRASFADXXNN-UHFFFAOYSA-N flavin mononucleotide Natural products OP(=O)(O)OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O FVTCRASFADXXNN-UHFFFAOYSA-N 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
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- 229910052742 iron Inorganic materials 0.000 description 2
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- 235000015097 nutrients Nutrition 0.000 description 2
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- 235000019192 riboflavin Nutrition 0.000 description 2
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- 235000019231 riboflavin-5'-phosphate Nutrition 0.000 description 2
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- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
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- 150000004688 heptahydrates Chemical class 0.000 description 1
- 150000007857 hydrazones Chemical class 0.000 description 1
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 229960002163 hydrogen peroxide Drugs 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 150000003893 lactate salts Chemical class 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229940073475 lysozyme hydrochloride Drugs 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- JQAACYUZYRBHGG-UHFFFAOYSA-L magnesium pidolate Chemical compound [Mg+2].[O-]C(=O)C1CCC(=O)N1.[O-]C(=O)C1CCC(=O)N1 JQAACYUZYRBHGG-UHFFFAOYSA-L 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 229940055036 mycobacterium phlei Drugs 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 229960005222 phenazone Drugs 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000013094 purity test Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 229960001153 serine Drugs 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- HFQQZARZPUDIFP-UHFFFAOYSA-M sodium;2-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O HFQQZARZPUDIFP-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000012134 supernatant fraction Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- HZSAJDVWZRBGIF-UHFFFAOYSA-M thiamine(1+) monophosphate(2-) Chemical compound CC1=C(CCOP([O-])([O-])=O)SC=[N+]1CC1=CN=C(C)N=C1N HZSAJDVWZRBGIF-UHFFFAOYSA-M 0.000 description 1
- 150000003609 titanium compounds Chemical class 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 125000002256 xylenyl group Chemical class C1(C(C=CC=C1)C)(C)* 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP53073619A JPS5910190B2 (ja) | 1978-06-17 | 1978-06-17 | 新規な乳酸オキシダ−ゼの製造法 |
| GB8009090A GB2042722B (en) | 1978-06-17 | 1979-06-14 | Determination of lactic acid |
| GB7920724A GB2025426B (en) | 1978-06-17 | 1979-06-14 | Lactateoxidase |
| CA329,879A CA1130739A (en) | 1978-06-17 | 1979-06-15 | Lactate oxidase, process for manufacture thereof and analytical method and kit for the use of the same |
| FR7915374A FR2428648B1 (en, 2012) | 1978-06-17 | 1979-06-15 | |
| US06/049,560 US4237222A (en) | 1978-06-17 | 1979-06-18 | Lactate oxidase process for the manufacture thereof and analytical method and kit for the use of the same |
| DE2924470A DE2924470C2 (de) | 1978-06-17 | 1979-06-18 | Lactatoxidase und ihre Herstellung |
| DE19792954503 DE2954503A1 (en, 2012) | 1978-06-17 | 1979-06-18 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP53073619A JPS5910190B2 (ja) | 1978-06-17 | 1978-06-17 | 新規な乳酸オキシダ−ゼの製造法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1792585A Division JPS6128386A (ja) | 1985-02-01 | 1985-02-01 | 新規な乳酸オキシダ−ゼおよびこれを用いた分析法、分析用キツト |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5576A JPS5576A (en) | 1980-01-05 |
| JPS5910190B2 true JPS5910190B2 (ja) | 1984-03-07 |
Family
ID=13523519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP53073619A Expired JPS5910190B2 (ja) | 1978-06-17 | 1978-06-17 | 新規な乳酸オキシダ−ゼの製造法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4237222A (en, 2012) |
| JP (1) | JPS5910190B2 (en, 2012) |
| CA (1) | CA1130739A (en, 2012) |
| DE (2) | DE2924470C2 (en, 2012) |
| FR (1) | FR2428648B1 (en, 2012) |
| GB (2) | GB2042722B (en, 2012) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USRE32016E (en) * | 1976-12-10 | 1985-10-29 | Eastman Kodak Company | Analysis of lactic acid or lactate using lactate oxidase |
| JPS5512918U (en, 2012) * | 1978-07-10 | 1980-01-26 | ||
| US4467811A (en) * | 1979-08-02 | 1984-08-28 | Children's Hospital Medical Center | Method of polarographic analysis of lactic acid and lactate |
| US4322496A (en) * | 1980-04-17 | 1982-03-30 | Eastman Kodak Company | Inhibition of lactate oxidase |
| JPS59140900A (ja) * | 1983-01-28 | 1984-08-13 | Toyo Jozo Co Ltd | 新規な酵素的高感度測定法 |
| JPS642597A (en) * | 1987-06-24 | 1989-01-06 | Sanwa Kagaku Kenkyusho Co Ltd | Test specimen for measuring lactic acid |
| DE3821077A1 (de) * | 1988-02-12 | 1989-08-24 | David Diagnostics Inc | Verfahren zur bestimmung des sauerstoffgehaltes in biologischen fluessigsubstraten und vorrichtung zur durchfuehrung des verfahrens |
| US5510244A (en) * | 1992-01-30 | 1996-04-23 | Kanzaki Paper Manufacturing Co., Ltd. | Apparatus and method for assaying optical isomers |
| IT1279043B1 (it) * | 1995-05-24 | 1997-12-04 | Co Ri Al Scpa | Metodo per la determinazione di acido lattico in materiali organici di interesse alimentare e biosensore per l'esecuzione di tale metodo |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK678474A (en, 2012) * | 1974-02-15 | 1975-10-13 | Hoffmann La Roche | |
| US4166763A (en) | 1976-12-10 | 1979-09-04 | Eastman Kodak Company | Analysis of lactic acid or lactate using lactate oxidase |
-
1978
- 1978-06-17 JP JP53073619A patent/JPS5910190B2/ja not_active Expired
-
1979
- 1979-06-14 GB GB8009090A patent/GB2042722B/en not_active Expired
- 1979-06-14 GB GB7920724A patent/GB2025426B/en not_active Expired
- 1979-06-15 FR FR7915374A patent/FR2428648B1/fr not_active Expired
- 1979-06-15 CA CA329,879A patent/CA1130739A/en not_active Expired
- 1979-06-18 US US06/049,560 patent/US4237222A/en not_active Expired - Lifetime
- 1979-06-18 DE DE2924470A patent/DE2924470C2/de not_active Expired
- 1979-06-18 DE DE19792954503 patent/DE2954503A1/de active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| FR2428648A1 (en, 2012) | 1980-01-11 |
| US4237222A (en) | 1980-12-02 |
| GB2025426B (en) | 1982-09-02 |
| GB2025426A (en) | 1980-01-23 |
| DE2924470A1 (de) | 1980-01-03 |
| GB2042722B (en) | 1983-07-20 |
| CA1130739A (en) | 1982-08-31 |
| FR2428648B1 (en, 2012) | 1985-03-29 |
| DE2924470C2 (de) | 1989-07-20 |
| DE2954503A1 (en, 2012) | 1986-03-20 |
| GB2042722A (en) | 1980-09-24 |
| JPS5576A (en) | 1980-01-05 |
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