JPS5856694A - 抗核抗体測定用試薬 - Google Patents
抗核抗体測定用試薬Info
- Publication number
- JPS5856694A JPS5856694A JP15066881A JP15066881A JPS5856694A JP S5856694 A JPS5856694 A JP S5856694A JP 15066881 A JP15066881 A JP 15066881A JP 15066881 A JP15066881 A JP 15066881A JP S5856694 A JPS5856694 A JP S5856694A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antigen
- reagent
- insolubilized
- antinuclear
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 36
- 230000003460 anti-nuclear Effects 0.000 title claims abstract description 27
- 238000003556 assay Methods 0.000 title abstract 2
- 239000000427 antigen Substances 0.000 claims abstract description 43
- 102000036639 antigens Human genes 0.000 claims abstract description 43
- 108091007433 antigens Proteins 0.000 claims abstract description 43
- 238000002372 labelling Methods 0.000 claims abstract description 20
- 238000006243 chemical reaction Methods 0.000 claims abstract description 18
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 17
- 239000004033 plastic Substances 0.000 claims abstract description 9
- 229920003023 plastic Polymers 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims abstract description 4
- 238000003018 immunoassay Methods 0.000 claims abstract description 3
- 238000005259 measurement Methods 0.000 claims description 21
- SRNWOUGRCWSEMX-KEOHHSTQSA-N ADP-beta-D-ribose Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)OC[C@H]1O[C@@H](O)[C@H](O)[C@@H]1O SRNWOUGRCWSEMX-KEOHHSTQSA-N 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000004793 Polystyrene Substances 0.000 abstract description 2
- 229920002223 polystyrene Polymers 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract 2
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 27
- 108020004414 DNA Proteins 0.000 description 20
- 239000000243 solution Substances 0.000 description 10
- 102000053602 DNA Human genes 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 206010029719 Nonspecific reaction Diseases 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 6
- 239000007790 solid phase Substances 0.000 description 6
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 5
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000003127 radioimmunoassay Methods 0.000 description 5
- 230000027455 binding Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 210000004408 hybridoma Anatomy 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- PWJFNRJRHXWEPT-UHFFFAOYSA-N ADP ribose Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OCC(O)C(O)C(O)C=O)C(O)C1O PWJFNRJRHXWEPT-UHFFFAOYSA-N 0.000 description 3
- 108060003951 Immunoglobulin Proteins 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 244000309466 calf Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 102000018358 immunoglobulin Human genes 0.000 description 3
- 238000005497 microtitration Methods 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 2
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 238000000692 Student's t-test Methods 0.000 description 2
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000012353 t test Methods 0.000 description 2
- XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004721 Polyphenylene oxide Substances 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 101150104365 Tomt gene Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002583 anti-histone Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920001692 polycarbonate urethane Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000570 polyether Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000012857 radioactive material Substances 0.000 description 1
- 239000002964 rayon Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- -1 sodium nitride Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 201000000596 systemic lupus erythematosus Diseases 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15066881A JPS5856694A (ja) | 1981-09-25 | 1981-09-25 | 抗核抗体測定用試薬 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15066881A JPS5856694A (ja) | 1981-09-25 | 1981-09-25 | 抗核抗体測定用試薬 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5856694A true JPS5856694A (ja) | 1983-04-04 |
| JPH0440662B2 JPH0440662B2 (enrdf_load_stackoverflow) | 1992-07-03 |
Family
ID=15501862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15066881A Granted JPS5856694A (ja) | 1981-09-25 | 1981-09-25 | 抗核抗体測定用試薬 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5856694A (enrdf_load_stackoverflow) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6488155A (en) * | 1987-06-22 | 1989-04-03 | Ruijiana State Univ Found The | Immunological inspection for detecting antibody against antigen |
| JPH0192660A (ja) * | 1987-01-30 | 1989-04-11 | Nippon Dpc Corp | 生物学的液体中の抗ｄｎａ抗体価の測定法および測定用キット |
| JPH03167473A (ja) * | 1989-11-08 | 1991-07-19 | Nippon Dpc Corp | 抗ｄｎａ抗体結合活性測定法および測定用キット |
| JPH03218462A (ja) * | 1990-10-30 | 1991-09-26 | Nippon Dpc Corp | 標準曲線における抗原結合率と抗二本鎖ｄｎａ抗体価の比例関係を改善するためのｓｌｅ疾患の検知法及び検知用キット |
| WO2005040798A1 (ja) * | 2003-10-29 | 2005-05-06 | Eisai Co., Ltd. | アルツハイマー病の診断方法 |
| CN104007266A (zh) * | 2013-02-27 | 2014-08-27 | 中国医学科学院基础医学研究所 | 含有6-n-甲基赖氨酸残基的抗原在制备辅助诊断系统性红斑狼疮试剂中的应用 |
-
1981
- 1981-09-25 JP JP15066881A patent/JPS5856694A/ja active Granted
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0192660A (ja) * | 1987-01-30 | 1989-04-11 | Nippon Dpc Corp | 生物学的液体中の抗ｄｎａ抗体価の測定法および測定用キット |
| JPS6488155A (en) * | 1987-06-22 | 1989-04-03 | Ruijiana State Univ Found The | Immunological inspection for detecting antibody against antigen |
| JPH03167473A (ja) * | 1989-11-08 | 1991-07-19 | Nippon Dpc Corp | 抗ｄｎａ抗体結合活性測定法および測定用キット |
| JPH03218462A (ja) * | 1990-10-30 | 1991-09-26 | Nippon Dpc Corp | 標準曲線における抗原結合率と抗二本鎖ｄｎａ抗体価の比例関係を改善するためのｓｌｅ疾患の検知法及び検知用キット |
| WO2005040798A1 (ja) * | 2003-10-29 | 2005-05-06 | Eisai Co., Ltd. | アルツハイマー病の診断方法 |
| CN104007266A (zh) * | 2013-02-27 | 2014-08-27 | 中国医学科学院基础医学研究所 | 含有6-n-甲基赖氨酸残基的抗原在制备辅助诊断系统性红斑狼疮试剂中的应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0440662B2 (enrdf_load_stackoverflow) | 1992-07-03 |
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