JPS58160861A - アミラ−ゼ活性測定電極 - Google Patents
アミラ−ゼ活性測定電極Info
- Publication number
- JPS58160861A JPS58160861A JP57044297A JP4429782A JPS58160861A JP S58160861 A JPS58160861 A JP S58160861A JP 57044297 A JP57044297 A JP 57044297A JP 4429782 A JP4429782 A JP 4429782A JP S58160861 A JPS58160861 A JP S58160861A
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- amylase
- glucose
- immobilized
- god
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000004382 Amylase Substances 0.000 title claims abstract description 29
- 102000013142 Amylases Human genes 0.000 title claims abstract description 28
- 108010065511 Amylases Proteins 0.000 title claims abstract description 28
- 235000019418 amylase Nutrition 0.000 title claims abstract description 28
- 230000000694 effects Effects 0.000 title claims abstract description 27
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims abstract description 30
- 102000004190 Enzymes Human genes 0.000 claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 18
- 229940088598 enzyme Drugs 0.000 claims abstract description 18
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052697 platinum Inorganic materials 0.000 claims abstract description 15
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims abstract description 9
- 102100022624 Glucoamylase Human genes 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 238000001514 detection method Methods 0.000 claims abstract description 5
- 108010015776 Glucose oxidase Proteins 0.000 claims abstract description 4
- 239000004366 Glucose oxidase Substances 0.000 claims abstract description 4
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims abstract description 4
- 108010028144 alpha-Glucosidases Proteins 0.000 claims abstract description 4
- 229940116332 glucose oxidase Drugs 0.000 claims abstract description 4
- 235000019420 glucose oxidase Nutrition 0.000 claims abstract description 4
- 239000000126 substance Substances 0.000 claims abstract description 4
- 239000007795 chemical reaction product Substances 0.000 claims description 6
- 230000003100 immobilizing effect Effects 0.000 claims description 2
- 108090000854 Oxidoreductases Proteins 0.000 claims 1
- 102000004316 Oxidoreductases Human genes 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 26
- 239000008103 glucose Substances 0.000 abstract description 26
- 102000004139 alpha-Amylases Human genes 0.000 abstract description 22
- 108090000637 alpha-Amylases Proteins 0.000 abstract description 22
- 229940024171 alpha-amylase Drugs 0.000 abstract description 22
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 abstract description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 abstract description 2
- 235000012208 gluconic acid Nutrition 0.000 abstract description 2
- 239000000174 gluconic acid Substances 0.000 abstract description 2
- 230000002452 interceptive effect Effects 0.000 abstract 2
- 239000000376 reactant Substances 0.000 abstract 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- -1 are immobilized Proteins 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 description 10
- 238000005259 measurement Methods 0.000 description 9
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000011148 porous material Substances 0.000 description 4
- 238000006911 enzymatic reaction Methods 0.000 description 3
- 229910052740 iodine Inorganic materials 0.000 description 3
- 239000011630 iodine Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229940079832 sodium starch glycolate Drugs 0.000 description 3
- 229920003109 sodium starch glycolate Polymers 0.000 description 3
- 239000008109 sodium starch glycolate Substances 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 108010093096 Immobilized Enzymes Proteins 0.000 description 2
- FTNIPWXXIGNQQF-UHFFFAOYSA-N Maltopentose Chemical compound OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(OC3C(OC(OC4C(OC(O)C(O)C4O)CO)C(O)C3O)CO)C(O)C2O)CO)C(O)C1O FTNIPWXXIGNQQF-UHFFFAOYSA-N 0.000 description 2
- 208000016222 Pancreatic disease Diseases 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 2
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 208000024691 pancreas disease Diseases 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 229940032147 starch Drugs 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 229940116269 uric acid Drugs 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57044297A JPS58160861A (ja) | 1982-03-18 | 1982-03-18 | アミラ−ゼ活性測定電極 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57044297A JPS58160861A (ja) | 1982-03-18 | 1982-03-18 | アミラ−ゼ活性測定電極 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58160861A true JPS58160861A (ja) | 1983-09-24 |
| JPH0332741B2 JPH0332741B2 (enrdf_load_stackoverflow) | 1991-05-14 |
Family
ID=12687567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57044297A Granted JPS58160861A (ja) | 1982-03-18 | 1982-03-18 | アミラ−ゼ活性測定電極 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58160861A (enrdf_load_stackoverflow) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4547280A (en) * | 1983-11-28 | 1985-10-15 | Hitachi, Ltd. | Maltose sensor |
| CN108548858A (zh) * | 2018-06-22 | 2018-09-18 | 桂林中辉科技发展有限公司 | 一种快速测量淀粉酶的电化学试纸及其制备与检测方法 |
-
1982
- 1982-03-18 JP JP57044297A patent/JPS58160861A/ja active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4547280A (en) * | 1983-11-28 | 1985-10-15 | Hitachi, Ltd. | Maltose sensor |
| CN108548858A (zh) * | 2018-06-22 | 2018-09-18 | 桂林中辉科技发展有限公司 | 一种快速测量淀粉酶的电化学试纸及其制备与检测方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0332741B2 (enrdf_load_stackoverflow) | 1991-05-14 |
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