JPS5668391A - Glucose-6-phosphate dehydrogenase and its preparation - Google Patents
Glucose-6-phosphate dehydrogenase and its preparationInfo
- Publication number
- JPS5668391A JPS5668391A JP14485879A JP14485879A JPS5668391A JP S5668391 A JPS5668391 A JP S5668391A JP 14485879 A JP14485879 A JP 14485879A JP 14485879 A JP14485879 A JP 14485879A JP S5668391 A JPS5668391 A JP S5668391A
- Authority
- JP
- Japan
- Prior art keywords
- enzyme
- glucose
- activity
- bacillus
- coenzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 title abstract 2
- 102100031126 6-phosphogluconolactonase Human genes 0.000 title 1
- 108010029731 6-phosphogluconolactonase Proteins 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 abstract 5
- 108090000790 Enzymes Proteins 0.000 abstract 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 4
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 abstract 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 2
- 210000005056 cell body Anatomy 0.000 abstract 2
- 239000005515 coenzyme Substances 0.000 abstract 2
- 229930024421 Adenine Natural products 0.000 abstract 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 abstract 1
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 241001037822 Bacillus bacterium Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 abstract 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 abstract 1
- VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 102000002794 Glucosephosphate Dehydrogenase Human genes 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 239000000287 crude extract Substances 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229960003966 nicotinamide Drugs 0.000 abstract 1
- 235000005152 nicotinamide Nutrition 0.000 abstract 1
- 239000011570 nicotinamide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Emergency Medicine (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: A bacterium belonging to Bacillus is cultured and the cell bodies are extracted to produce a heat-resistant enzyme that can keep higher than 80% activity of that of untreated enzyme even after the treatment in a buffef at about 50°C for about 15min, thue producing an inexpensive enzyme that acts on a coenzyme and kees high stability for a long period of time.
CONSTITUTION: A Bacillus bacterium such as Bacillus stearothermophilus is cultured, the culture mixture is centrifuged, the resultant cell bodies are suspended in a phosphoric acid buffer, the cells are crushed by means of a French press or the like and the crushed cell walls are removed to give a crude extract. The extract is purified to give the objective glucose-phosphate dehydrogenase. The resultant enzyme can keep about 80W100% activity of that of untreated one even after the treatment of heating in a phosphoric acid buffer solution at about 50°C for 15min. It is used as an enzyme with stably high activity for a long period of time for determining glucose, glucose-6-phosphate, hexonase or the like by using inexpensive nicotinamide adenin dinucleotide as a coenzyme in medical and food fields.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14485879A JPS5668391A (en) | 1979-11-07 | 1979-11-07 | Glucose-6-phosphate dehydrogenase and its preparation |
| CA000363960A CA1156570A (en) | 1979-11-07 | 1980-11-04 | Glucose-6-phosphate dehydrogenase and process for preparation thereof |
| IT50089/80A IT1146060B (en) | 1979-11-07 | 1980-11-05 | GLUCOSE-6-PHOSPHATE-DEHYDROGENASE AND PROCEDURE FOR PREPARING IT |
| DE19803041744 DE3041744A1 (en) | 1979-11-07 | 1980-11-05 | GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND METHOD FOR THE PRODUCTION THEREOF |
| DK471880A DK471880A (en) | 1979-11-07 | 1980-11-06 | GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND PROCEDURES FOR PREPARING THEREOF |
| SE8007818A SE8007818L (en) | 1979-11-07 | 1980-11-06 | GLUCOS-6-PHOSPHATEDE HYDROGEN AND PROCEDURES FOR PREPARING THEREOF |
| GB8035669A GB2066261B (en) | 1979-11-07 | 1980-11-06 | Glucose-6-phosphate dehydrogenase and process for preparation thereof |
| FR8023926A FR2472608B1 (en) | 1979-11-07 | 1980-11-07 | NOVEL GLUCOSE-6-PHOSPHATE-DEHYDROGENASE, PREPARATION METHOD THEREOF AND COENZYMATIC COMPOSITIONS CONTAINING THE SAME |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14485879A JPS5668391A (en) | 1979-11-07 | 1979-11-07 | Glucose-6-phosphate dehydrogenase and its preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5668391A true JPS5668391A (en) | 1981-06-09 |
| JPS6343079B2 JPS6343079B2 (en) | 1988-08-26 |
Family
ID=15372034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14485879A Granted JPS5668391A (en) | 1979-11-07 | 1979-11-07 | Glucose-6-phosphate dehydrogenase and its preparation |
Country Status (8)
| Country | Link |
|---|---|
| JP (1) | JPS5668391A (en) |
| CA (1) | CA1156570A (en) |
| DE (1) | DE3041744A1 (en) |
| DK (1) | DK471880A (en) |
| FR (1) | FR2472608B1 (en) |
| GB (1) | GB2066261B (en) |
| IT (1) | IT1146060B (en) |
| SE (1) | SE8007818L (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5971687A (en) * | 1982-10-12 | 1984-04-23 | Toyobo Co Ltd | Stable composition containing glucose-6-phosphate dehydrogenase |
| JPS6152282A (en) * | 1984-08-22 | 1986-03-14 | Toyobo Co Ltd | Thermostable glucose-6-phosphate dehydrogenase |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2742281B2 (en) * | 1988-12-29 | 1998-04-22 | 旭化成工業株式会社 | Glucose-6-phosphate dehydrogenase gene |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5326389A (en) * | 1976-08-24 | 1978-03-11 | Secr Defence Brit | Production of glycerokinase enzyme |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1096797A (en) * | 1976-11-19 | 1981-03-03 | Kenichiro Takayama | Process for the preparation of choline oxidase by fermentation |
-
1979
- 1979-11-07 JP JP14485879A patent/JPS5668391A/en active Granted
-
1980
- 1980-11-04 CA CA000363960A patent/CA1156570A/en not_active Expired
- 1980-11-05 DE DE19803041744 patent/DE3041744A1/en active Granted
- 1980-11-05 IT IT50089/80A patent/IT1146060B/en active
- 1980-11-06 DK DK471880A patent/DK471880A/en unknown
- 1980-11-06 GB GB8035669A patent/GB2066261B/en not_active Expired
- 1980-11-06 SE SE8007818A patent/SE8007818L/en unknown
- 1980-11-07 FR FR8023926A patent/FR2472608B1/en not_active Expired
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5326389A (en) * | 1976-08-24 | 1978-03-11 | Secr Defence Brit | Production of glycerokinase enzyme |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5971687A (en) * | 1982-10-12 | 1984-04-23 | Toyobo Co Ltd | Stable composition containing glucose-6-phosphate dehydrogenase |
| JPS6347439B2 (en) * | 1982-10-12 | 1988-09-21 | Toyo Boseki | |
| JPS6152282A (en) * | 1984-08-22 | 1986-03-14 | Toyobo Co Ltd | Thermostable glucose-6-phosphate dehydrogenase |
| JPH03995B2 (en) * | 1984-08-22 | 1991-01-09 | Toyo Boseki |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6343079B2 (en) | 1988-08-26 |
| DK471880A (en) | 1981-05-08 |
| DE3041744A1 (en) | 1981-05-21 |
| CA1156570A (en) | 1983-11-08 |
| FR2472608A1 (en) | 1981-07-03 |
| IT8050089A0 (en) | 1980-11-05 |
| FR2472608B1 (en) | 1985-12-06 |
| IT1146060B (en) | 1986-11-12 |
| DE3041744C2 (en) | 1990-07-12 |
| GB2066261B (en) | 1983-09-21 |
| SE8007818L (en) | 1981-05-08 |
| GB2066261A (en) | 1981-07-08 |
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