JPS5621590A - Novel glycerol dehydrogenase, and its preparation - Google Patents
Novel glycerol dehydrogenase, and its preparationInfo
- Publication number
- JPS5621590A JPS5621590A JP9645979A JP9645979A JPS5621590A JP S5621590 A JPS5621590 A JP S5621590A JP 9645979 A JP9645979 A JP 9645979A JP 9645979 A JP9645979 A JP 9645979A JP S5621590 A JPS5621590 A JP S5621590A
- Authority
- JP
- Japan
- Prior art keywords
- gdh
- glycerol
- purified
- medium containing
- bacterial cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To obtain a novel and highly active glycerol dehydrogenase (GDH) by culturing a GDH-producing strain belonging to Bacillus genus in a medium containing glycerol, and extracting the GDH accumulated in the bacterial cells.
CONSTITUTION: G-1 Bacteria belonging to Bacillus genus are cultured in a medium containing 2% of glycerol to promote the DGH-producing activity and a Mn salt as an essential salt component, at 30°C for about 24hrs under agitation. After separation and grinding of the cultured bacterial cells, the objective enzyme is extracted, and purified by conventional process such as salting-out, etc. to obtain crude GDH, which can be purified by the adsorption and desorption with an ion exchange material. The purified GDH obtained by this process in high efficiency and useful for the clinical examination has the following characteristics: molecular weight, 85,000±5,000; optimum pH range for operation, 9.5W10; stable pH range, 5.0W9.0; optimum temperature for operation, about 45°C; stable temperature, ≤50°C; Km value for glycerol, 1.33×10-2M, Km value for NAD, 1.28×10-4M.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54096459A JPS6012027B2 (en) | 1979-07-27 | 1979-07-27 | Novel glycerol dehydrogenase and its production method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP54096459A JPS6012027B2 (en) | 1979-07-27 | 1979-07-27 | Novel glycerol dehydrogenase and its production method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5621590A true JPS5621590A (en) | 1981-02-28 |
JPS6012027B2 JPS6012027B2 (en) | 1985-03-29 |
Family
ID=14165604
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54096459A Expired JPS6012027B2 (en) | 1979-07-27 | 1979-07-27 | Novel glycerol dehydrogenase and its production method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6012027B2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59156280A (en) * | 1983-02-28 | 1984-09-05 | Rikagaku Kenkyusho | Glycerol dehydrogenase and its preparation |
JPH04345820A (en) * | 1991-05-22 | 1992-12-01 | Sanyo Kokusaku Pulp Co Ltd | Manufacture of polystyrene resin foamed sheet |
-
1979
- 1979-07-27 JP JP54096459A patent/JPS6012027B2/en not_active Expired
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59156280A (en) * | 1983-02-28 | 1984-09-05 | Rikagaku Kenkyusho | Glycerol dehydrogenase and its preparation |
JPH0372272B2 (en) * | 1983-02-28 | 1991-11-18 | Rikagaku Kenkyusho | |
JPH04345820A (en) * | 1991-05-22 | 1992-12-01 | Sanyo Kokusaku Pulp Co Ltd | Manufacture of polystyrene resin foamed sheet |
Also Published As
Publication number | Publication date |
---|---|
JPS6012027B2 (en) | 1985-03-29 |
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