JPS5645199A - Reagent composition for determination of free fatty acid - Google Patents

Reagent composition for determination of free fatty acid

Info

Publication number
JPS5645199A
JPS5645199A JP12229879A JP12229879A JPS5645199A JP S5645199 A JPS5645199 A JP S5645199A JP 12229879 A JP12229879 A JP 12229879A JP 12229879 A JP12229879 A JP 12229879A JP S5645199 A JPS5645199 A JP S5645199A
Authority
JP
Japan
Prior art keywords
coenzyme
group
free fatty
fatty acid
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP12229879A
Other languages
Japanese (ja)
Inventor
Toshiro Kikuchi
Noboru Mitsuhida
Minoru Ando
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Priority to JP12229879A priority Critical patent/JPS5645199A/en
Priority to DE19803005379 priority patent/DE3005379A1/en
Publication of JPS5645199A publication Critical patent/JPS5645199A/en
Pending legal-status Critical Current

Links

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To recover sufficiently high color developing sensitivity and to determine the amount of a free fatty acid accurately, by the combined use of a group containing acyl-coenzyme A synthetase, coenzyme A, etc., and a group containing a peroxidase, a color developing agent, ete. CONSTITUTION:A reagent for the determination of a free fatty acid is prepared from the group I containing acyl-coenzyme A synthetase, coenzyme A, adenosine triphosphate, and magnesium and the group II composed of a peroxidase, a color developing agent and a thiol-group blocking agent. The compounds of the group I are pref. acyl-coenzyme A synthetase, pyrophosphatase, pyruvic acid oxidase, coenzyme A, pyruvic acid, thiamine pyrophosphate, adenosine triphosphate, and magnesium, and the thiol-group blocking agent is pref. monohaloacetic acid or its acid amide.
JP12229879A 1979-02-13 1979-09-21 Reagent composition for determination of free fatty acid Pending JPS5645199A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP12229879A JPS5645199A (en) 1979-09-21 1979-09-21 Reagent composition for determination of free fatty acid
DE19803005379 DE3005379A1 (en) 1979-02-13 1980-02-13 Quantitative analysis of free fatty acid - by conversion to acyl coenzyme=A which is then oxidised with formation of hydrogen peroxide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12229879A JPS5645199A (en) 1979-09-21 1979-09-21 Reagent composition for determination of free fatty acid

Publications (1)

Publication Number Publication Date
JPS5645199A true JPS5645199A (en) 1981-04-24

Family

ID=14832487

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12229879A Pending JPS5645199A (en) 1979-02-13 1979-09-21 Reagent composition for determination of free fatty acid

Country Status (1)

Country Link
JP (1) JPS5645199A (en)

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