JPS56144098A - Measurement of enzyme or substrate by luminescence of organism - Google Patents

Measurement of enzyme or substrate by luminescence of organism

Info

Publication number
JPS56144098A
JPS56144098A JP4889980A JP4889980A JPS56144098A JP S56144098 A JPS56144098 A JP S56144098A JP 4889980 A JP4889980 A JP 4889980A JP 4889980 A JP4889980 A JP 4889980A JP S56144098 A JPS56144098 A JP S56144098A
Authority
JP
Japan
Prior art keywords
enzyme
luminescence
reaction
glycerol
organism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP4889980A
Other languages
Japanese (ja)
Inventor
Takashi Yamada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Optical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Optical Co Ltd filed Critical Olympus Optical Co Ltd
Priority to JP4889980A priority Critical patent/JPS56144098A/en
Publication of JPS56144098A publication Critical patent/JPS56144098A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: To measure an enzyme or substrate qualitatively or quantitatively, by adding an enzyme, substrate, or coenzyme to a test sample to cause an enzyme reaction, introducing finally the reaction to a reaction process wherein ATP (adenosine triphosphate) is formed or consumed, adding luciferin and luciferase to the reaction, followed by measuring an amount of luminescence caused by an organism.
CONSTITUTION: In the case where an amount of triglyceride is measured, when it is reacted with an enzyme hydrolyzing glycerol ester, it is decomposed into glycerol and a fatty acid. ATP and an enzyme rearranging phosphoric acid group are added to the prepared glycerol, to give glycerol-3-phosphate and adenosine diphosphate. An amount of excess ATP is subjected to luminescence by an organism with luciferin and luciferase, and the luminescence is measured.
COPYRIGHT: (C)1981,JPO&Japio
JP4889980A 1980-04-14 1980-04-14 Measurement of enzyme or substrate by luminescence of organism Pending JPS56144098A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4889980A JPS56144098A (en) 1980-04-14 1980-04-14 Measurement of enzyme or substrate by luminescence of organism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4889980A JPS56144098A (en) 1980-04-14 1980-04-14 Measurement of enzyme or substrate by luminescence of organism

Publications (1)

Publication Number Publication Date
JPS56144098A true JPS56144098A (en) 1981-11-10

Family

ID=12816105

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4889980A Pending JPS56144098A (en) 1980-04-14 1980-04-14 Measurement of enzyme or substrate by luminescence of organism

Country Status (1)

Country Link
JP (1) JPS56144098A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63233799A (en) * 1987-03-23 1988-09-29 Eisai Co Ltd Method for measuring pyruvate kinase and reagent
US4902190A (en) * 1987-09-14 1990-02-20 Cascade Corporation Fork positioning attachment for lift trucks
US6672823B2 (en) 2001-09-11 2004-01-06 Cascade Corporation Fork positioner for facilitating replacement of forks on lift trucks
US7909563B2 (en) 2004-11-30 2011-03-22 Cascade Corporation Fork positioner
US8403618B2 (en) 2004-11-30 2013-03-26 Cascade Corporation Lift truck load handler

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63233799A (en) * 1987-03-23 1988-09-29 Eisai Co Ltd Method for measuring pyruvate kinase and reagent
US4902190A (en) * 1987-09-14 1990-02-20 Cascade Corporation Fork positioning attachment for lift trucks
US6672823B2 (en) 2001-09-11 2004-01-06 Cascade Corporation Fork positioner for facilitating replacement of forks on lift trucks
US7008167B2 (en) 2001-09-11 2006-03-07 Cascade Corporation Fork positioner for facilitating replacement of forks on lift trucks
US7909563B2 (en) 2004-11-30 2011-03-22 Cascade Corporation Fork positioner
US8403618B2 (en) 2004-11-30 2013-03-26 Cascade Corporation Lift truck load handler

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