JPS5648895A - Measurement of nonelectron pair bond fatty acid - Google Patents
Measurement of nonelectron pair bond fatty acidInfo
- Publication number
- JPS5648895A JPS5648895A JP12295879A JP12295879A JPS5648895A JP S5648895 A JPS5648895 A JP S5648895A JP 12295879 A JP12295879 A JP 12295879A JP 12295879 A JP12295879 A JP 12295879A JP S5648895 A JPS5648895 A JP S5648895A
- Authority
- JP
- Japan
- Prior art keywords
- nonelectron
- fatty acid
- acid
- pair bond
- bond fatty
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000014113 dietary fatty acids Nutrition 0.000 title abstract 5
- 239000000194 fatty acid Substances 0.000 title abstract 5
- 229930195729 fatty acid Natural products 0.000 title abstract 5
- 150000004665 fatty acids Chemical class 0.000 title abstract 5
- 238000005259 measurement Methods 0.000 title 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 abstract 5
- 229950006790 adenosine phosphate Drugs 0.000 abstract 5
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 abstract 3
- 239000005516 coenzyme A Substances 0.000 abstract 3
- 229940093530 coenzyme a Drugs 0.000 abstract 3
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 abstract 3
- GANZODCWZFAEGN-UHFFFAOYSA-N 5-mercapto-2-nitro-benzoic acid Chemical compound OC(=O)C1=CC(S)=CC=C1[N+]([O-])=O GANZODCWZFAEGN-UHFFFAOYSA-N 0.000 abstract 2
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 abstract 2
- 102000005870 Coenzyme A Ligases Human genes 0.000 abstract 2
- 108010011449 Long-chain-fatty-acid-CoA ligase Proteins 0.000 abstract 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-N diphosphoric acid Chemical compound OP(O)(=O)OP(O)(O)=O XPPKVPWEQAFLFU-UHFFFAOYSA-N 0.000 abstract 2
- -1 myokinase Chemical compound 0.000 abstract 2
- 229940005657 pyrophosphoric acid Drugs 0.000 abstract 2
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 abstract 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 abstract 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 1
- 102000002281 Adenylate kinase Human genes 0.000 abstract 1
- 108020000543 Adenylate kinase Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 229960001456 adenosine triphosphate Drugs 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To measure a nonelectron pair bond fatty acid quantitatively, by treating a nonelectron pair bond fatty acid, coenzyme A (Co A), etc. with an enzyme and decomposer, followed by converting the remaining Co A to 2-nitro-5-mercaptobenzoic acid, which is determined optically.
CONSTITUTION: Acyl coenzyme A synthetase (ACS) and a decomposer for adenosine-5'-triphosphate (AMP), e.g., myokinase, etc. are added to and brought into contact with a specimen containing a nonelectron pair bond fatty acid (NEFA), coenzyme A (Co A) and AMP in pH7W9 at room temperature to 40°C. Consequently, the above-mentioned substances which is treated in the specimen are converted enzymically to acyl coenzyme A, adenosine-5'-phosphate (AMP), and pyrophosphoric acid, and simultaneously AMP and pyrophosphoric acid are transformed or decomposed. The remaining Co A in the treatment is reacted with 5,5'-dithiobis- (2-nitrobenzoic acid) to give 2-nitro-5-mercaptobenzoic acid (TNB-), which is determined optically to measure a nonelectron pair bond fatty acid. The reactions are shown by the formulas I and II.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12295879A JPS5648895A (en) | 1979-09-25 | 1979-09-25 | Measurement of nonelectron pair bond fatty acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12295879A JPS5648895A (en) | 1979-09-25 | 1979-09-25 | Measurement of nonelectron pair bond fatty acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5648895A true JPS5648895A (en) | 1981-05-02 |
Family
ID=14848829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12295879A Pending JPS5648895A (en) | 1979-09-25 | 1979-09-25 | Measurement of nonelectron pair bond fatty acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5648895A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5950086U (en) * | 1982-09-25 | 1984-04-03 | 大日本印刷株式会社 | magnetic recording medium |
| JPS62133287U (en) * | 1986-02-12 | 1987-08-22 | ||
| FR2699930A1 (en) * | 1992-12-24 | 1994-07-01 | Kabore Paul | Bioluminescent determn of adenosine phosphates |
-
1979
- 1979-09-25 JP JP12295879A patent/JPS5648895A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5950086U (en) * | 1982-09-25 | 1984-04-03 | 大日本印刷株式会社 | magnetic recording medium |
| JPS62133287U (en) * | 1986-02-12 | 1987-08-22 | ||
| FR2699930A1 (en) * | 1992-12-24 | 1994-07-01 | Kabore Paul | Bioluminescent determn of adenosine phosphates |
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