JPS54113383A - Method and apparatus for measuring enzyme - Google Patents

Method and apparatus for measuring enzyme

Info

Publication number
JPS54113383A
JPS54113383A JP2047478A JP2047478A JPS54113383A JP S54113383 A JPS54113383 A JP S54113383A JP 2047478 A JP2047478 A JP 2047478A JP 2047478 A JP2047478 A JP 2047478A JP S54113383 A JPS54113383 A JP S54113383A
Authority
JP
Japan
Prior art keywords
value
indicates
equation
measured
absorption
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2047478A
Other languages
Japanese (ja)
Other versions
JPS5654583B2 (en
Inventor
Takao Kawamoto
Yoichi Komurasaki
Kiyokazu Nakano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP2047478A priority Critical patent/JPS54113383A/en
Publication of JPS54113383A publication Critical patent/JPS54113383A/en
Publication of JPS5654583B2 publication Critical patent/JPS5654583B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/272—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

PURPOSE:To measure the quantity of enzyme by arranging at least three optical absorption detectors in consecsutive positions with respect to the proceeding of an enzyme reaction so that the detected value for use in calculation may be selected in accordance with the values obtained by the first and second detectors. CONSTITUTION:In case glutamic acid oxalacetic transminase (GOT) in serum is to be measured by the production rate method using malic dehydrogenase enzyme (MDH) and nicotinic acid amide adenine dinucleotide of reduction type, the concentration of NADH2 is measured at the times t1, t2 and t3 after the start of the enzyme reaction to obtain the measured absorption values d1, d2 and d3. Then, the preset absorption variation level DELTAd0 is compared with the value obtained from equation II so that, if the latter is higher, the value of equation II is used as the value DELTAE of equation I and so that, if the latter is lower, the value of equation III is used as DELTAE of equation I thereby to calculate the value of GOT. Here, V indicates the liquid quantity of the final reaction; TF indicates a temperature coefficient; epsilon indicates the absorption coefficient at the measured wavelength of NADH2; v indicates the quantity of serum; and a indicates the length of the optical path.
JP2047478A 1978-02-23 1978-02-23 Method and apparatus for measuring enzyme Granted JPS54113383A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2047478A JPS54113383A (en) 1978-02-23 1978-02-23 Method and apparatus for measuring enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2047478A JPS54113383A (en) 1978-02-23 1978-02-23 Method and apparatus for measuring enzyme

Publications (2)

Publication Number Publication Date
JPS54113383A true JPS54113383A (en) 1979-09-04
JPS5654583B2 JPS5654583B2 (en) 1981-12-26

Family

ID=12028095

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2047478A Granted JPS54113383A (en) 1978-02-23 1978-02-23 Method and apparatus for measuring enzyme

Country Status (1)

Country Link
JP (1) JPS54113383A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56155835A (en) * 1980-05-02 1981-12-02 Olympus Optical Co Ltd Component analyzing method
JPS58156855A (en) * 1982-03-15 1983-09-17 Konishiroku Photo Ind Co Ltd Analytical method and device
JPS6113160A (en) * 1984-06-28 1986-01-21 Konishiroku Photo Ind Co Ltd Biochemical analyser
JPS6113158A (en) * 1984-06-28 1986-01-21 Konishiroku Photo Ind Co Ltd Biochemical analyser

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7309628B2 (en) 2020-01-15 2023-07-18 株式会社モリタ製作所 CAP, IMAGING DEVICE, DATA GENERATION SYSTEM, AND DATA GENERATION METHOD

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56155835A (en) * 1980-05-02 1981-12-02 Olympus Optical Co Ltd Component analyzing method
JPH0224337B2 (en) * 1980-05-02 1990-05-29 Olympus Optical Co
JPS58156855A (en) * 1982-03-15 1983-09-17 Konishiroku Photo Ind Co Ltd Analytical method and device
JPS6113160A (en) * 1984-06-28 1986-01-21 Konishiroku Photo Ind Co Ltd Biochemical analyser
JPS6113158A (en) * 1984-06-28 1986-01-21 Konishiroku Photo Ind Co Ltd Biochemical analyser
JPH045141B2 (en) * 1984-06-28 1992-01-30
JPH0439624B2 (en) * 1984-06-28 1992-06-30

Also Published As

Publication number Publication date
JPS5654583B2 (en) 1981-12-26

Similar Documents

Publication Publication Date Title
TW200606416A (en) Method of and apparatus for determining the amount of impurity in gas
AU6481898A (en) Method and apparatus for correcting ambient temperature effect in biosensors
DE69221306D1 (en) METHOD FOR PHOTOMETRIC (IN VITRO) DETERMINATION OF THE CONTENT OF ANALYTIC IN A SAMPLE
MX247272B (en) Method for the online determination of hydrogen peroxide.
CA2127207A1 (en) Blood Culture Sensor Station Utilizing Two Distinct Light Sources
JPS54113383A (en) Method and apparatus for measuring enzyme
DE3482805D1 (en) METHOD FOR DETERMINING AMMONIA.
EP0267308A3 (en) Method and apparatus for dynamic power differential calorimetry
JPS5536759A (en) Measuring method of kallikrein in human urine
JPS55119046A (en) Method and apparatus for measuring concentration of components of electrolyzed solution of chromic acid
JPS5353348A (en) Optical fiber length measuring method and apparatus
JPS56103351A (en) Method for measuring impurity concentration of semiconductor
JPS54134482A (en) Analysis of neodymium in yag crystal
JPS57184969A (en) Measuring method for immunity of homo-pth
JPS53106184A (en) Automatic absorption meter
JPS542179A (en) Low concentration radioactive gas measuring apparatus provided with enrichment rate corrector
JPS5354105A (en) Heating temperature controlling method for strip
JPS6491051A (en) Flow type density measuring apparatus
JPS53134491A (en) Tester for oxygen concentration detector
JPS5557135A (en) Light absoption intensity quantization method for dehydrogeneration enzyme using tetrasolium chloride and its compound
JPS5367485A (en) Direct light measuring analytical method used plural
JPS531043A (en) Automatic range setter of automatic exhausting gas analyzer
JPS51121391A (en) A method ion contineously measuring cyan ion and a eletrode for measur ing cyan ion,
JPS5614157A (en) Fluorescent substrate and measuring method using it
JPS56128458A (en) Measuring method for dimethylpolysiloxane in steam curing light weight air bubble concrete