JPS55108298A - Determining method of xanthine oxidase substrate by use of xanthine oxidase originating from microorganism - Google Patents

Determining method of xanthine oxidase substrate by use of xanthine oxidase originating from microorganism

Info

Publication number
JPS55108298A
JPS55108298A JP1382879A JP1382879A JPS55108298A JP S55108298 A JPS55108298 A JP S55108298A JP 1382879 A JP1382879 A JP 1382879A JP 1382879 A JP1382879 A JP 1382879A JP S55108298 A JPS55108298 A JP S55108298A
Authority
JP
Japan
Prior art keywords
xanthine oxidase
microorganism
substrate
originating
xanthine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1382879A
Other languages
Japanese (ja)
Inventor
Toru Nakanishi
Yozo Machida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Priority to JP1382879A priority Critical patent/JPS55108298A/en
Priority to DE7979900990T priority patent/DE2965849D1/en
Priority to PCT/JP1979/000219 priority patent/WO1980000454A1/en
Priority to EP79900990A priority patent/EP0016845B1/en
Priority to US06/201,067 priority patent/US4341868A/en
Publication of JPS55108298A publication Critical patent/JPS55108298A/en
Pending legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: A xanthine oxidase originating from microorganism is made to act on a xanthine oxidase substrate and the hydrogen peroxide formed is led to a pigment system to measure the absorption by the pigment, whereby the substrate is determined.
CONSTITUTION: A xanthine oxidase originating from microorganism, e.g., obtained by the cultivation of a microorganism belonging to Enterobacter, is made to act on a xanthine oxidase substrate, e.g., xanthine, hypoxanthine, 2-hydroxypurine or guanine, at a suitable buffer solution to form hydrogen peroxide. Peroxidase and a coloring agent are added to the hydrogen peroxide to form a pigment and the visible light absorption is measured to determine the xanthine oxidase substrate.
COPYRIGHT: (C)1980,JPO&Japio
JP1382879A 1978-08-18 1979-02-10 Determining method of xanthine oxidase substrate by use of xanthine oxidase originating from microorganism Pending JPS55108298A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP1382879A JPS55108298A (en) 1979-02-10 1979-02-10 Determining method of xanthine oxidase substrate by use of xanthine oxidase originating from microorganism
DE7979900990T DE2965849D1 (en) 1978-08-18 1979-08-17 Method and test composition for the determination of a substrate for xanthine-oxidase, including a novel xanthine-oxidase and method for the preparation thereof
PCT/JP1979/000219 WO1980000454A1 (en) 1978-08-18 1979-08-17 Method of measuring substrate of xanthine oxidase,and composition for the determination
EP79900990A EP0016845B1 (en) 1978-08-18 1980-03-25 Method and test composition for the determination of a substrate for xanthine-oxidase, including a novel xanthine-oxidase and method for the preparation thereof
US06/201,067 US4341868A (en) 1978-08-18 1980-04-18 Method and test composition for the determination of the substrate for xanthine oxidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1382879A JPS55108298A (en) 1979-02-10 1979-02-10 Determining method of xanthine oxidase substrate by use of xanthine oxidase originating from microorganism

Publications (1)

Publication Number Publication Date
JPS55108298A true JPS55108298A (en) 1980-08-20

Family

ID=11844126

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1382879A Pending JPS55108298A (en) 1978-08-18 1979-02-10 Determining method of xanthine oxidase substrate by use of xanthine oxidase originating from microorganism

Country Status (1)

Country Link
JP (1) JPS55108298A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03262498A (en) * 1990-03-12 1991-11-22 Nichirei Corp Freshness determination of fishes and shellfishes and determination device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50113289A (en) * 1974-02-14 1975-09-05

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS50113289A (en) * 1974-02-14 1975-09-05

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03262498A (en) * 1990-03-12 1991-11-22 Nichirei Corp Freshness determination of fishes and shellfishes and determination device

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