JPH11507348A - ペプチドの修飾および精製システムおよび方法 - Google Patents
ペプチドの修飾および精製システムおよび方法Info
- Publication number
- JPH11507348A JPH11507348A JP9501828A JP50182897A JPH11507348A JP H11507348 A JPH11507348 A JP H11507348A JP 9501828 A JP9501828 A JP 9501828A JP 50182897 A JP50182897 A JP 50182897A JP H11507348 A JPH11507348 A JP H11507348A
- Authority
- JP
- Japan
- Prior art keywords
- peptide
- ultrafiltration
- reactor
- liquid
- washing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00281—Individual reactor vessels
- B01J2219/00283—Reactor vessels with top opening
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00353—Pumps
- B01J2219/00355—Pumps peristaltic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00479—Means for mixing reactants or products in the reaction vessels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.限外濾過装置、 この限外濾過装置に流体接続されている反応器、および この限外濾過装置と反応器との間に流体相互接続されているポンプ を備えた、ペプチドの連続液相複合化およびその精製用のシステム。 2.前記限外濾過装置と相互接続されている逆洗浄液貯槽をさらに備えている 、請求の範囲第1項記載の閉鎖システム。 3.前記反応器、限外濾過装置および逆洗浄液貯槽の間に少なくとも1つの三 方向バルブが選択的に流体相互接続されて配置されている、請求の範囲第1項記 載の閉鎖システム。 4.前記限外濾過装置がラセン透析濾過カートリッジを備えている、請求の範 囲第1項記載の閉鎖システム。 5.前記反応器が前記限外濾過装置からの精製濃縮生成物の逆洗浄液を受ける 容器ともなる、請求の範囲第1項記載のシステム。 6.下記工程からなる閉鎖液体システム内で第1のペプチドを第2のペプチド に複合化する方法。 (a) 第1のペプチドを液体反応混合物中で第2のペプチドに複合化して、限外 濾過手段で複合化ペプチドを保持し、限外濾過手段を通過する未複合化ペプチド は廃棄することにより両者を分離できるように、複合化および未複合化ペプチド の混合物を形成し、 (b) 複合化分子と未複合化分子とを含む液体反応混合物を限外濾過手段に送り 込み、 (c) 限外濾過手段内で単離された複合化ペプチドを適当な脱塩用溶液または他 の緩衝液を用いて、限外濾過に付すことにより洗浄し、 (d) 限外濾過手段を緩衝液で逆洗浄して、洗浄された複合化ペプチドを限外濾 過手段から取り出し、 (e) 複合化ペプチドが残りの未複合化ペプチドを実質的に含まなくなるまで工 程(c)および(d)を繰り返して、複合化ペプチドを精製し、そして (f) 限外濾過手段から複合化ペプチドを回収する。 7.液体混合物中のペプチド成分を精製する方法であって、この液体混合物を 限外濾過装置内に備えられた半透膜上に送って、ペプチドを選択的に膜上に保持 し;膜上に保持されたペプチドを洗浄し;膜からペプチドを洗浄液を用いて逆洗 浄し;保持されたペプチドを含有する洗浄液を回収することからなる方法。 8.ペプチドを半透膜上に送る前に、ペプチドを活性化、複合化、または修飾 する、請求の範囲第7項記載のペプチドの精製方法。 9.洗浄および逆洗浄の工程を少なくとも1回反復する請求の範囲第7項記載 のペプチドの精製方法。 10.限外濾過装置が透析濾過手段を含んでいる、請求の範囲第7項記載のペプ チドまたはタンパク質の精製方法。 11.半透膜が5,000; 15,000; 30,000 および100,000 ダルトンよりなる群から 選ばれた分子量カットオフを持つ、請求の範囲第7項記載のペプチドまたはタン パク質の精製方法。 12.ペプチドをタンパク質免疫原に複合化し、限外濾過手段により精製するペ プチド−タンパク質免疫原複合体の製造方法において、ペプチドとタンパク質免 疫原との複合化を閉鎖システム内の液体混合物中において行い、複合化および未 複合化のペプチドおよびタンパク質免疫原の混合物を生成させ、この混合物を未 複合化ペプチドおよびタンパク質免疫原からペプチド−タンパク質免疫原複合体 を分離するように限外濾過、洗浄および逆洗浄を繰り返し受けさせることからな る改良方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/484,740 | 1995-06-07 | ||
US08/484,740 US6359114B1 (en) | 1995-06-07 | 1995-06-07 | System for method for the modification and purification of proteins |
PCT/US1996/009525 WO1996040733A1 (en) | 1995-06-07 | 1996-06-07 | A system and method for the modification and purification of peptides |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH11507348A true JPH11507348A (ja) | 1999-06-29 |
JP3752695B2 JP3752695B2 (ja) | 2006-03-08 |
Family
ID=23925411
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50182897A Expired - Lifetime JP3752695B2 (ja) | 1995-06-07 | 1996-06-07 | ペプチドの修飾および精製システムおよび方法 |
Country Status (10)
Country | Link |
---|---|
US (2) | US6359114B1 (ja) |
EP (1) | EP0832097B1 (ja) |
JP (1) | JP3752695B2 (ja) |
AT (1) | ATE207931T1 (ja) |
AU (1) | AU698179B2 (ja) |
DE (1) | DE69616546T2 (ja) |
DK (1) | DK0832097T3 (ja) |
ES (1) | ES2163025T3 (ja) |
PT (1) | PT832097E (ja) |
WO (1) | WO1996040733A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002542927A (ja) * | 1999-04-28 | 2002-12-17 | アベシア・リミテッド | 反応器 |
Families Citing this family (30)
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US6359114B1 (en) | 1995-06-07 | 2002-03-19 | Aphton Corp. | System for method for the modification and purification of proteins |
JP2000504692A (ja) * | 1996-02-08 | 2000-04-18 | アフトン・コーポレーション | 胃腸癌治療用の免疫学的方法 |
US6123848A (en) * | 1997-02-14 | 2000-09-26 | Warner-Jenkinson Company, Inc. | Ultrafiltration method for purifying water-insoluble aluminum hydrates |
GB2348155B (en) * | 1997-02-14 | 2000-11-01 | Warner Jenkinson Co Inc | Apparatus for purifying water-insoluble compounds |
DK0968291T3 (da) | 1997-02-21 | 2004-06-07 | Genentech Inc | Antistoffragment-polymerkonjugater |
US20040001842A1 (en) * | 1997-05-12 | 2004-01-01 | Dov Michaeli | Immunogenic compositions to the CCK-B/gastrin receptor and methods for the treatment of tumors |
ES2227833T3 (es) * | 1997-05-12 | 2005-04-01 | Aphton Corporation | Composiciones inmunogenicas para el receptor cck-b/gastrina y metodos para el tratamiento de tumores. |
NL1006071C1 (nl) * | 1997-05-16 | 1998-11-17 | Chemferm Vof | Werkwijze voor het verwijderen van hoogmoleculaire verbindingen uit een processtroom. |
JP2002515458A (ja) * | 1998-05-15 | 2002-05-28 | アフトン・コーポレーシヨン | 高ガストリン血症の予防および処置 |
US20030068326A1 (en) * | 1998-05-15 | 2003-04-10 | Aphton Corporation | Method for the treatment of gastroesophageal reflux disease |
US6602414B2 (en) * | 2000-03-30 | 2003-08-05 | Formulations Pro | Molecule separation device and method combining multiple filtration media |
CA2441228A1 (en) * | 2001-03-23 | 2002-10-03 | Aphton Corporation | Combination treatment of pancreatic cancer |
US20090191232A1 (en) * | 2001-05-04 | 2009-07-30 | Gevas Philip C | Combination therapy for the treatment of tumors |
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JP2005535663A (ja) * | 2002-07-03 | 2005-11-24 | アフトン コーポレーション | リポソームワクチン |
US20050169979A1 (en) * | 2002-07-03 | 2005-08-04 | Dov Michaeli | Liposomal vaccine |
US20040247661A1 (en) * | 2002-07-03 | 2004-12-09 | Dov Michaeli | Liposomal vaccine |
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US20060020119A1 (en) * | 2004-03-29 | 2006-01-26 | Stephen Grimes | Monoclonal antibodies to gastrin hormone |
JP2008513536A (ja) * | 2004-09-22 | 2008-05-01 | レセプター バイオロジックス インコーポレイテッド | プロガストリンに対するモノクローナル抗体 |
JP2013523210A (ja) * | 2010-03-26 | 2013-06-17 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 滅菌方法 |
EP2682168A1 (en) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
RU2768003C2 (ru) | 2013-03-08 | 2022-03-22 | Джензим Корпорейшн | Интегрированное непрерывное производство терапевтических белковых лекарственных веществ |
TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
TWI671312B (zh) | 2014-01-17 | 2019-09-11 | 美商健臻公司 | 無菌層析法及製法 |
EP3015542A1 (de) * | 2015-05-07 | 2016-05-04 | Bayer Technology Services GmbH | Modulare anlage und verfahren zur kontinuierlichen, keimreduzierten produktion und/oder aufbereitung eines produktes |
CA3066756A1 (en) | 2017-06-15 | 2018-12-20 | Cancer Advances Inc. | Compositions and methods for inducing humoral and cellular immunities against tumors and cancer |
BR112021003420A2 (pt) | 2018-08-31 | 2021-05-18 | Genzyme Corporation | resina cromatográfica estéril e uso da mesma em processos de manufatura |
EP3939691B1 (en) * | 2020-07-13 | 2023-11-22 | Sartorius Stedim Biotech GmbH | Device assembly for producing bioconjugates |
WO2023034490A2 (en) * | 2021-09-02 | 2023-03-09 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | A hierarchically organized system for contemporaneous fractionation of multiple fractions |
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-
1995
- 1995-06-07 US US08/484,740 patent/US6359114B1/en not_active Expired - Lifetime
-
1996
- 1996-06-07 ES ES96918342T patent/ES2163025T3/es not_active Expired - Lifetime
- 1996-06-07 AT AT96918342T patent/ATE207931T1/de not_active IP Right Cessation
- 1996-06-07 WO PCT/US1996/009525 patent/WO1996040733A1/en active IP Right Grant
- 1996-06-07 JP JP50182897A patent/JP3752695B2/ja not_active Expired - Lifetime
- 1996-06-07 PT PT96918342T patent/PT832097E/pt unknown
- 1996-06-07 EP EP96918342A patent/EP0832097B1/en not_active Expired - Lifetime
- 1996-06-07 DE DE69616546T patent/DE69616546T2/de not_active Expired - Lifetime
- 1996-06-07 AU AU61029/96A patent/AU698179B2/en not_active Expired
- 1996-06-07 DK DK96918342T patent/DK0832097T3/da active
-
2002
- 2002-03-15 US US10/098,690 patent/US20020095028A1/en not_active Abandoned
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002542927A (ja) * | 1999-04-28 | 2002-12-17 | アベシア・リミテッド | 反応器 |
Also Published As
Publication number | Publication date |
---|---|
DE69616546T2 (de) | 2002-05-02 |
WO1996040733A1 (en) | 1996-12-19 |
PT832097E (pt) | 2002-04-29 |
ATE207931T1 (de) | 2001-11-15 |
JP3752695B2 (ja) | 2006-03-08 |
AU6102996A (en) | 1996-12-30 |
EP0832097B1 (en) | 2001-10-31 |
US20020095028A1 (en) | 2002-07-18 |
US6359114B1 (en) | 2002-03-19 |
AU698179B2 (en) | 1998-10-29 |
ES2163025T3 (es) | 2002-01-16 |
DK0832097T3 (da) | 2001-11-19 |
DE69616546D1 (de) | 2001-12-06 |
EP0832097A1 (en) | 1998-04-01 |
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