JPH10507081A - シュワン細胞の分離および培養 - Google Patents
シュワン細胞の分離および培養Info
- Publication number
- JPH10507081A JPH10507081A JP8534101A JP53410196A JPH10507081A JP H10507081 A JPH10507081 A JP H10507081A JP 8534101 A JP8534101 A JP 8534101A JP 53410196 A JP53410196 A JP 53410196A JP H10507081 A JPH10507081 A JP H10507081A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- schwann cells
- culture medium
- culture
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.Rse/Ax1受容体活性化剤を含む第一の有糸分裂促進剤および第二 の有糸分裂促進剤を含むシュワン細胞を培養するための血清不含培養培地。 2.Rse/Ax1受容体活性化剤がgas6を含む請求項1の培養培地。 3.第二の有糸分裂促進剤がヘレグリンを含む請求項1の培養培地。 4.さらに培養培地のcAMPレベルを上昇させる薬剤を含む請求項1の培 養培地。 5.さらにフォルスコリンを含む請求項1の培養培地。 6.さらに鉄源を含む請求項1の培養培地。 7.鉄源がトランスフェリンを含む請求項6の培養培地。 8.さらにインスリンまたはインスリン様増殖因子−I(IGF−I)を含 む請求項1の培養培地。 9.さらにビタミンEを含む請求項1の培養培地。 10.さらにプロテアーゼ阻害剤を含む請求項1の培養培地。 11.Rse/Ax1受容体活性化剤を含む第一の有糸分裂促進剤および第二 の有糸分裂促進剤が共にシュワン細胞の生存または増殖を強化するために有効な 量で存在する血清不含の培養培地中でシュワン細胞を培養することを含む細胞培 養におけるシュワン細胞の生存または増殖を増強する方法。 12.シュワン細胞がヒトのシュワン細胞を含む請求項11の方法。 13.Rse/Ax1受容体活性化剤がgas6を含む請求項11の方法。 14.第二の有糸分裂促進剤がヘレグリンを含む請求項11の方法。 15.シュワン細胞の生存と増殖を強化する請求項11の方法。 16.培養をラミニンの存在下に実行する請求項11の方法。 17.シュワン細胞をラミニン被覆培養プレート中で培養することを含む請求 項16の方法。 18.(a)シュワン細胞が脱ミエリン化を起こすように有糸分裂促進剤を含 む血清不含培養培地中で約1日から約5日までの期間シュワン細胞を含む神経組 織をプレインキュベーションすること、および (b)脱ミエリン化を起こしたシュワン細胞を請求項1の培養培地中で培養す ること、 を含むシュワン細胞を分離するための方法。 19.神経組織を約10分間から約100分間プロテアーゼと接触させる段階 が段階(a)に先行する請求項18の方法。 20.段階(a)に先行して神経組織をコラーゲナーゼおよびディスパーゼに 接触させる請求項19の方法。 21.培養培地がヘレグリン、インスリンおよびフォルスコリンを含む請求項 18の方法。 22.段階(b)が脱ミエリン化を起こしたシュワン細胞をラミニン被覆培養 プレートで培養することを含む請求項18の方法。 23.ヒトのシュワン細胞および医薬的に許容される担体を含み、本質的に血 清不含な組成物。 24.ヒトのシュワン細胞を血清不含培養培地中に含む組成物であって、その 培養培地がさらに有糸分裂促進剤を含むもの。 25.請求項12に従って培養されたヒトのシュワン細胞。 26.請求項12に従って培養されたヒトのシュワン細胞の有効量を哺乳類に 投与することを含む哺乳類の処置法。 27.請求項23の組成物の有効量を哺乳類に投与することを含む哺乳類の処 置法。 28.その哺乳類が神経系に損傷を受けているものである請求項27の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/438,863 US5849585A (en) | 1995-05-10 | 1995-05-10 | Isolating and culturing Schwann cells |
US08/438,863 | 1995-05-10 | ||
PCT/US1996/005846 WO1996035776A1 (en) | 1995-05-10 | 1996-04-26 | Isolating and culturing schwann cells |
Publications (2)
Publication Number | Publication Date |
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JPH10507081A true JPH10507081A (ja) | 1998-07-14 |
JP3410103B2 JP3410103B2 (ja) | 2003-05-26 |
Family
ID=23742342
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP53410196A Expired - Lifetime JP3410103B2 (ja) | 1995-05-10 | 1996-04-26 | シュワン細胞の分離および培養 |
Country Status (11)
Country | Link |
---|---|
US (1) | US5849585A (ja) |
EP (1) | EP0826035B1 (ja) |
JP (1) | JP3410103B2 (ja) |
AT (1) | ATE320483T1 (ja) |
AU (1) | AU714177B2 (ja) |
CA (1) | CA2218909C (ja) |
DE (1) | DE69635924T2 (ja) |
IL (1) | IL118150A (ja) |
MX (1) | MX9708527A (ja) |
WO (1) | WO1996035776A1 (ja) |
ZA (1) | ZA963389B (ja) |
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JP2007508816A (ja) * | 2003-10-10 | 2007-04-12 | ゲ・ミン・ルイ | 細胞培養および組織培養プラットフォームのための組成物および方法 |
JP2008503201A (ja) * | 2003-11-21 | 2008-02-07 | アリーナ ファーマシューティカルズ, インコーポレイテッド | 嗅覚gpcrを産生するための方法 |
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US4443546A (en) * | 1980-07-07 | 1984-04-17 | The Beth Israel Hospital Association | Process and composition for propagating mammalian cells |
NZ206699A (en) * | 1982-12-30 | 1989-08-29 | Bio Response Inc | Process for the production of serum independent cell lines |
US4662884A (en) * | 1984-04-25 | 1987-05-05 | University Of Utah Research Foundation | Prostheses and methods for promoting nerve regeneration |
US5030225A (en) * | 1987-03-13 | 1991-07-09 | Brown University Research Foundation | Electrically-charged nerve guidance channels |
WO1990007007A1 (en) * | 1988-12-14 | 1990-06-28 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | Cell culture medium for human liver epithelial cell line |
US5082670A (en) * | 1988-12-15 | 1992-01-21 | The Regents Of The University Of California | Method of grafting genetically modified cells to treat defects, disease or damage or the central nervous system |
AU8649391A (en) * | 1990-08-15 | 1992-03-17 | University Of Miami | Autotransplantation of schwann cells to promote nervous system repair |
JPH0789908B2 (ja) * | 1991-02-28 | 1995-10-04 | 倉敷紡績株式会社 | 動物細胞培養用無血清培地 |
GB9107566D0 (en) * | 1991-04-10 | 1991-05-29 | Ludwig Inst Cancer Res | Glial mitogenic factors,their preparation and use |
US5530109A (en) * | 1991-04-10 | 1996-06-25 | Ludwig Institute For Cancer Research | DNA encoding glial mitogenic factors |
CH684573A5 (de) * | 1991-11-25 | 1994-10-31 | Inst Molecular Biology Inc | Verwendung von Wachstumsfaktoren zur Herstellung eines Arzneimittels für die Wachstumsförderung von Säugetiernerven. |
GB9217316D0 (en) * | 1992-08-14 | 1992-09-30 | Ludwig Inst Cancer Res | Schwann cell mitogenic factor,its preparation and use |
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1995
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1996
- 1996-04-26 MX MX9708527A patent/MX9708527A/es not_active IP Right Cessation
- 1996-04-26 EP EP96913200A patent/EP0826035B1/en not_active Expired - Lifetime
- 1996-04-26 AU AU55789/96A patent/AU714177B2/en not_active Ceased
- 1996-04-26 AT AT96913200T patent/ATE320483T1/de active
- 1996-04-26 JP JP53410196A patent/JP3410103B2/ja not_active Expired - Lifetime
- 1996-04-26 WO PCT/US1996/005846 patent/WO1996035776A1/en active IP Right Grant
- 1996-04-26 CA CA2218909A patent/CA2218909C/en not_active Expired - Fee Related
- 1996-04-26 DE DE69635924T patent/DE69635924T2/de not_active Expired - Lifetime
- 1996-04-29 ZA ZA9603389A patent/ZA963389B/xx unknown
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007508816A (ja) * | 2003-10-10 | 2007-04-12 | ゲ・ミン・ルイ | 細胞培養および組織培養プラットフォームのための組成物および方法 |
JP2008503201A (ja) * | 2003-11-21 | 2008-02-07 | アリーナ ファーマシューティカルズ, インコーポレイテッド | 嗅覚gpcrを産生するための方法 |
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IL118150A0 (en) | 1996-09-12 |
IL118150A (en) | 2000-08-31 |
CA2218909C (en) | 2010-06-29 |
US5849585A (en) | 1998-12-15 |
EP0826035B1 (en) | 2006-03-15 |
JP3410103B2 (ja) | 2003-05-26 |
DE69635924D1 (de) | 2006-05-11 |
DE69635924T2 (de) | 2006-12-14 |
WO1996035776A1 (en) | 1996-11-14 |
ATE320483T1 (de) | 2006-04-15 |
ZA963389B (en) | 1997-10-29 |
EP0826035A1 (en) | 1998-03-04 |
AU5578996A (en) | 1996-11-29 |
MX9708527A (es) | 1998-02-28 |
AU714177B2 (en) | 1999-12-23 |
CA2218909A1 (en) | 1996-11-14 |
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