JPH10506013A - ヒトの凝固第viii因子及びフォンビルブラント因子を発現するトランスジェニック動物 - Google Patents
ヒトの凝固第viii因子及びフォンビルブラント因子を発現するトランスジェニック動物Info
- Publication number
- JPH10506013A JPH10506013A JP8510993A JP51099396A JPH10506013A JP H10506013 A JPH10506013 A JP H10506013A JP 8510993 A JP8510993 A JP 8510993A JP 51099396 A JP51099396 A JP 51099396A JP H10506013 A JPH10506013 A JP H10506013A
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- protein
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/027—New breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0278—Humanized animals, e.g. knockin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/15—Humanized animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.組換えヒト第VIII因子蛋白質又はそれらの断片もしくは修飾体を発現し、該 発現した蛋白質を体液中に分泌する非ヒトトランスジェニック哺乳動物。 2.外因性の二本鎖DNA配列が安定的に組み込まれているゲノムを有する雌哺乳 動物を含む、請求項1に記載の動物において、該DNA配列が第VIII因子蛋白質又は それらの断片もしくは修飾体とシグナルペプチドとをコードするDNA配列に機能 的に結合した乳蛋白質プロモーターを含み、該プロモーターが乳腺細胞で特異的 に作用するものであり、該シグナルペプチドが該雌哺乳動物の乳中に新たに発現 した該第VIII因子を誘導し分泌させるのに有効なものである、動物。 3.ヒト第VIII因子が約2332個のアミノ酸残基からなるヘテロ二量体の糖蛋白質 を含み、該ヘテロ二量体の糖蛋白質が約80kDaのC−末端アミノ酸配列と約210kDa のN−末端アミノ酸配列を含む、請求項2に記載の動物。 4.ヒトフォンビルブラント因子蛋白質又はそれらの断片もしくは修飾体を発現 し、該蛋白質を体液中に分泌する非ヒトトランスジェニック哺乳動物。 5.外因性の二本鎖DNA配列が安定的に組み込まれているゲノムを有する雌哺乳 動物を含む、請求項4に記載の動物において、該DNA配列が組換えヒトフォンビル ブラント因子とシグナルペプチドとをコードするDNA配列に機能的に結合した乳 蛋白質プロモーターを含み、該プロモーターが乳腺細胞で特異的に作用するもの であり、該シグナルペプチドが該雌哺乳動物の乳中に新たに発現したフォンビル ブラント因子を誘導し分泌させるのに有効なものである、動物。 6.組換えヒト第VIII因子とフォンビルブラント因子とを同時に発現し、両蛋白 質を乳中に同時に分泌する非ヒトトランスジェニック雌哺乳動物。 7.乳蛋白質プロモーターが、乳清酸性蛋白質プロモーター、カゼインプロモー ター、ラクトアルブミンプロモーター、及びベーターラクトグロブリンプロモー ターからなる群より選択されるものである、請求項1〜6のいずれか一項に記載の 動物。 8.雌哺乳動物が、げっ歯動物、ウサギ、イヌ、ブタ、ヒツジ、ヤギ、ウマ、及 びウシからなる群より選択されるものである、請求項1〜6のいずれか一項に記載 の動物。 9.組換えヒト第VIII因子を製造する方法であって、 (a)外因性の二本鎖DNA配列がゲノムへ組み込まれていることを特徴とする非 ヒトトランスジェニック雌哺乳動物を提供する段階(該外因性のDNA配列は第VII I因子蛋白質とシグナルペプチドとをコードするDNA配列に機能的に結合した乳蛋 白質プロモーターを含み、該プロモーターは乳腺細胞で特異的に活性なものであ り、該シグナルペプチドは該哺乳動物の乳中に新たに発現した該第VIII因子を誘 導し分泌させるのに有効なものである)、 (b)該雌哺乳動物に乳汁を分泌させる段階、 (c)該雌哺乳動物から乳を採集する段階、及び (d)該乳から該蛋白質を単離する段階、 を含む、方法。 10.動物が第2の外因性の二本鎖DNA配列が安定的に組み込まれたグノムをさらに 有する請求項9に記載の方法において、該第2の外因性DNA配列がヒトフォンビル ブラント因子とシグナルペプチドとをコードするDNA配列に機能的に結合した乳 蛋白質プロモーターを含み、該プロモーターが乳腺細胞で特異的に作用するもの であり、該シグナルペプチドが該哺乳動物の乳中に新たに発現したフォンビルブ ラント因子を誘導するのに有効なものである、方法。 11.乳腺組織において組換えヒト第VIII因子蛋白質を産生し、乳中に該ポリペプ チドを分泌する能力のある非ヒトトランスジェニック雌哺乳動物を製造する方法 であって、 (a)該第VIII因子蛋白質とシグナルペプチドとをコードするDNA配列に機能的 に結合した乳蛋白質プロモーターを含む二本鎖DNA配列を提供する段階(該プロ モーターは乳腺細胞で特異的に作用するものであり、該シグナルペプチドは該哺 乳動物の乳中に新たに合成された第VIII因子を誘導するのに有効なものである) 、 (b)該二本鎖DNAを精製する段階、 (c)該精製された二本鎖DNAを雌哺乳動物の胚に注入する段階(該注入された DNA配列は、ゲノムに安定的に組み込まれた該注入されたDNAを有するトランスジ ェニック哺乳動物を製造するのに有効なものである)、及び (d)該動物で乳汁の分泌が誘導される期間まで該胚を分化させる段階、 を含む、方法。 12.外因性のDNA配列がプラスミドp225.11(ATCC受託番号 )に含まれる、請求 項9に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/308,518 US5880327A (en) | 1994-09-21 | 1994-09-21 | Transgenic mammals expressing human coagulation factor VIII |
US08/308,518 | 1994-09-21 | ||
PCT/US1995/011781 WO1996009377A1 (en) | 1994-09-21 | 1995-09-21 | Transgenic animals expressing human coagulation factor viii and von willebrand factor |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009188810A Division JP2010017184A (ja) | 1994-09-21 | 2009-08-18 | ヒトの凝固第viii因子及びフォンビルブラント因子を発現するトランスジェニック動物 |
Publications (1)
Publication Number | Publication Date |
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JPH10506013A true JPH10506013A (ja) | 1998-06-16 |
Family
ID=23194291
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8510993A Withdrawn JPH10506013A (ja) | 1994-09-21 | 1995-09-21 | ヒトの凝固第viii因子及びフォンビルブラント因子を発現するトランスジェニック動物 |
JP2009188810A Withdrawn JP2010017184A (ja) | 1994-09-21 | 2009-08-18 | ヒトの凝固第viii因子及びフォンビルブラント因子を発現するトランスジェニック動物 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009188810A Withdrawn JP2010017184A (ja) | 1994-09-21 | 2009-08-18 | ヒトの凝固第viii因子及びフォンビルブラント因子を発現するトランスジェニック動物 |
Country Status (8)
Country | Link |
---|---|
US (2) | US5880327A (ja) |
EP (2) | EP0807170B1 (ja) |
JP (2) | JPH10506013A (ja) |
AT (1) | ATE321785T1 (ja) |
CA (1) | CA2200610C (ja) |
DE (1) | DE69534907T2 (ja) |
ES (1) | ES2262143T3 (ja) |
WO (1) | WO1996009377A1 (ja) |
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FR2948665B1 (fr) | 2009-07-31 | 2011-09-23 | Lfb Biotechnologies | Procede pour la purification de proteines de la coagulation a domaine gla actives |
FR2948664B1 (fr) | 2009-07-31 | 2013-11-01 | Lfb Biotechnologies | Procede pour la purification de proteines de la coagulation a domaine gla |
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UA109884C2 (uk) | 2009-10-16 | 2015-10-26 | Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування | |
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US4757006A (en) * | 1983-10-28 | 1988-07-12 | Genetics Institute, Inc. | Human factor VIII:C gene and recombinant methods for production |
AU602487B2 (en) * | 1985-05-16 | 1990-10-18 | Meloy Laboratories, Inc. | Molecular cloning of cDNA for human factor VIIIR (von Willebrand factor) |
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GB8826446D0 (en) * | 1988-11-11 | 1988-12-14 | Agricultural & Food Res | Peptide production |
DE69027683T2 (de) * | 1989-12-01 | 1997-02-13 | Pharming Bv | Herstellung rekombinanter polypeptide durch rinder und transgene methoden |
US5288846A (en) * | 1990-10-19 | 1994-02-22 | The General Hospital Corporation | Cell specific gene regulators |
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US5880327A (en) * | 1994-09-21 | 1999-03-09 | American National Red Cross | Transgenic mammals expressing human coagulation factor VIII |
DE4435485C1 (de) * | 1994-10-04 | 1996-03-21 | Immuno Ag | Verfahren zur Gewinnung von hochreinem von Willebrand-Faktor |
-
1994
- 1994-09-21 US US08/308,518 patent/US5880327A/en not_active Expired - Lifetime
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1995
- 1995-09-21 CA CA2200610A patent/CA2200610C/en not_active Expired - Fee Related
- 1995-09-21 JP JP8510993A patent/JPH10506013A/ja not_active Withdrawn
- 1995-09-21 WO PCT/US1995/011781 patent/WO1996009377A1/en active IP Right Grant
- 1995-09-21 ES ES95933128T patent/ES2262143T3/es not_active Expired - Lifetime
- 1995-09-21 AT AT95933128T patent/ATE321785T1/de active
- 1995-09-21 DE DE69534907T patent/DE69534907T2/de not_active Expired - Lifetime
- 1995-09-21 EP EP95933128A patent/EP0807170B1/en not_active Expired - Lifetime
- 1995-09-21 EP EP06006431A patent/EP1739170A3/en not_active Withdrawn
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1999
- 1999-03-04 US US09/262,017 patent/US6255554B1/en not_active Expired - Fee Related
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- 2009-08-18 JP JP2009188810A patent/JP2010017184A/ja not_active Withdrawn
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EP0807170A1 (en) | 1997-11-19 |
EP0807170B1 (en) | 2006-03-29 |
US5880327A (en) | 1999-03-09 |
CA2200610C (en) | 2010-11-23 |
JP2010017184A (ja) | 2010-01-28 |
DE69534907T2 (de) | 2007-03-15 |
EP1739170A2 (en) | 2007-01-03 |
ATE321785T1 (de) | 2006-04-15 |
DE69534907D1 (de) | 2006-05-18 |
CA2200610A1 (en) | 1996-03-28 |
US6255554B1 (en) | 2001-07-03 |
EP1739170A3 (en) | 2007-03-07 |
ES2262143T3 (es) | 2006-11-16 |
WO1996009377A1 (en) | 1996-03-28 |
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