JPH10164987A - Cultivation method of plant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a safe and inexpensive cultivation method for plants capable of preventing the infiltration and propagation of saprophytic bacteria and pathogenic bacteria. SOLUTION: The seeds of radish sprouts are subjected to a lactic acid bacterium treatment in the clean state of the surfaces of the seeds by washing and swelling the seeds (S1, S2), by which the lactic acid bacteria are fixed to the surfaces of the seeds (S3). As a result, the surfaces of the radish sprouts maintain the state of covering the surfaces with the lactic acid bacteria at all times in the growth process of the radish sprouts which root and germinate afterward, by which the infiltration and propagation of the saprophytic bacteria and the pathogenic bacteria are suppressed. The surfaces of the radish sprouts are more surely coated with the lactic acid bacteria by spraying the lactic acid bacteria at need (S6, S8) after seeding (S5)) or at the time of growth in greenhouses.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for cultivating a plant,
More specifically, the present invention relates to a method for cultivating a plant for preventing invasion and propagation of various bacteria and pathogenic bacteria.

[0002]

2. Description of the Related Art In a cultivation process from a seed stage to a shipping stage, various cultivation environments of water, fertilizer, sunshine and the like can be artificially controlled. Is a typical example.

As an example of such hydroponic cultivation, a conventional cultivation method will be described with reference to FIG.

[0004] First, the seeds of the dried radish are washed (step S21), and the washed seeds are swelled by immersion in water or a cultivation solution (step S22).

[0005] Thus, the seeds are rooted (step S23). When the seeds have rooted, the seeds are sown in a dedicated cultivation container or the like (step S24).

Thereafter, the seeds germinate in a germination chamber or the like (step S25), and the germinated seeds are transferred to, for example, a cultivation house and grown there (step S26).

[0007] When the radish is grown to a stage where it can be shipped, it is provided for shipment (step S27).

[0008] The possibility of contamination by various germs and pathogens in the conventional plant cultivation method as described above includes contamination by bringing into a cultivation house by human limbs, clothes, and the like, and equipment used repeatedly in the cultivation process. From water and cultivation liquid, and the like.

In order to prevent such contamination of bacteria and pathogens, when entering the cultivation house, wash hands, change clothes, change shoes, etc.
Disinfect equipment and instruments using, for example, a formalin solution or a diluted solution of neutral calcium hypochlorite, and heat-treat water or cultivation liquid to about 90 degrees Celsius or irradiate with ultraviolet rays, ozone treatment, or ultrasonic waves. Performing processing and the like is conventionally known.

In the case of tomato, cucumber, melon, and the like, dry heat disinfection for maintaining seeds at 70 to 73 degrees Celsius for 3 to 4 days or hot water at 53 to 55 degrees Celsius for 20 to 30 minutes Hot water disinfection to soak is performed.

Furthermore, since the propagation rate of various germs and pathogens varies depending on the environment, means for controlling the propagation by adjusting the pH, temperature, type of ion, concentration of the cultivation liquid, and the like have been conventionally used. For example, in three leaves, the cultivation solution is twice the concentration of the standard solution (EC 4.8) in order to suppress the occurrence of root rot caused by Pythium spp.
Means, such as increasing the value, are used.

[0012]

However, in the conventional plant cultivation method as described above, despite careful attention, various germs and pathogens are present in the air. In addition, it was difficult to block the contamination of these germs and pathogens.

In order to cope with this, it is conceivable to create a completely sterile condition in the cultivation process. However, when this means is used, enormous costs are required for initial capital investment and subsequent maintenance of the equipment. It is difficult to provide economically inexpensive plants to the market.

The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a safe and inexpensive plant cultivation method capable of preventing invasion and propagation of various bacteria and pathogenic bacteria.

[0015]

Means for Solving the Problems In order to achieve the above object, a plant cultivation method according to a first aspect of the present invention provides a method for cultivating a cultivation environment in a cultivation process from a seed stage to a shipping stage. In a controllable plant cultivation method, the plant is washed, and fungi that are harmless to the human body are fixed on the surface of the plant and propagated, and the surface of the plant is coated with the fungus. .

The plant cultivation method according to the second invention is a method for cultivating a plant by hydroponics, wherein the plant is washed and fungi harmless to the human body are fixed on the surface of the plant and multiplied. And coating the surface of the plant with the fungus.

Further, the plant cultivation method according to the third invention is a method for cultivating a plant by hydroponics, wherein the plant is washed and fungi harmless to the human body are mixed into the culture solution. By supplying the fungus to the plant, the fungus is fixed on the surface including the root of the plant and propagated, and the fungus covers the surface including the root of the plant.

The plant cultivation method according to a fourth aspect of the present invention is the plant cultivation method according to the first, second, or third aspect, wherein the fungus is obtained by mixing two or more kinds of fungi harmless to the human body. And wherein the mixed fungi proliferate in symbiosis with each other.

According to a fifth aspect of the present invention, in the method for cultivating a plant according to the first, second or third aspect, the fungus is a lactic acid bacterium.

According to a sixth aspect of the present invention, in the method for cultivating a plant according to the first, second or third aspect, the fungus is fixed on the surface of the plant by washing the plant. After a long time.

The plant cultivation method according to a seventh aspect of the present invention is the plant cultivation method according to the first, second, or third aspect of the present invention, wherein the plant to which the fungus is fixed by washing is set in a seed state, rooting state. Or a germinated seed state, or a rooted and germinated seed state.

[0022]

Embodiments of the present invention will be described below with reference to the drawings. 1 and 2 show an embodiment of the present invention. FIG. 1 is a flowchart showing a method for cultivating radish, and FIG. 2 is a graph showing the growth of lactic acid bacteria and coliforms over time in the method for cultivating radish. It is a chart showing an example of a change in breeding state.

This embodiment is a method for cultivating a plant capable of artificially controlling a cultivation environment such as water, fertilizer, and sunshine in a cultivation process from a seed stage to a shippable stage.
A cultivation method by hydroponic cultivation, more specifically, a cultivation method by hydroponic cultivation is applied, and radish is applied as a plant cultivated by this cultivation method.

The method for cultivating the radish radish in such an embodiment will be described with reference to FIG.

First, the seeds of the radish are washed (step S1). In this washing treatment, unnecessary proteins, amino acids, dust, etc. attached to the seeds are removed, and various germs and pathogens that may be attached to the seeds are removed.

Next, the washed seeds are swollen by immersing them in water or a cultivation solution (step S2).

Then, in the state where the surface of the seed is clean as described above, lactic acid bacteria treatment is performed to fix lactic acid bacteria on the surface of the seed (step S3). This lactic acid bacterium is harmless or beneficial to the human body.

As the lactic acid bacteria treatment, for example, an aqueous solution prepared by putting lactic acid bacteria in water and stirring the solution is
This is performed by soaking the seeds.

After the seeds are washed in step S1, the swelling treatment in step S2 is also performed in a clean state, and then the lactic acid bacteria treatment in step S3 is performed within a short time. desirable. This prevents germs and pathogens from attaching back to the seeds,
This is because the surface of the seed can be covered with only lactic acid bacteria.

The lactic acid bacteria treatment to be carried out for the first time includes the seed state, the rooted seed state, the germinated seed state, or the rooting and germination, which are the initial stages of the cultivation process as described above. It is desirable to carry out the treatment in the condition of a seed that has been used. Although it is possible to perform the first lactic acid bacteria treatment in the middle and late stages of the cultivation process, it is necessary to wash the lactic acid bacteria before the treatment, and after the radish grows, clean the entire surface. Is difficult. In addition, at the seed or at a stage similar to the seed, the surface is unlikely to be damaged even if the washing is performed, but it is easy to be damaged after the radish grows.

After performing the swelling treatment described above,
The seed roots (step S4). Once the seeds have rooted,
For example, the seeds are sown in a dedicated cultivation container or the like having a cultivation section of a shipping unit (step S5), and a cultivation liquid is sprayed.

Then, in order to more surely cover the surface of the rooted seed with the lactic acid bacteria, the lactic acid bacteria in the state of the aqueous solution as described above are sprayed (step S).
6).

Thereafter, the seeds germinate in a germination room or the like (step S7), and the germinated seeds are transferred to, for example, a cultivation house and grown there (step S8). In this growing process, lactic acid bacteria may be sprayed as needed.

In the process of growing radish, water and nutrients are generally given by appropriately spraying a culture solution. Therefore, as a method for spraying the lactic acid bacteria, it is preferable to use means for mixing the lactic acid bacteria into the culture solution.

By using such means, it is not necessary to separately perform the treatment of spraying the culture solution and the treatment of spraying the lactic acid bacteria, so that the operation can be simplified and the culture solution can be applied to the bottom of the cultivation container. Due to the accumulation, it becomes possible to promote the establishment of lactic acid bacteria on the radish, especially on the root.

In this way, the radish that has rooted and germinated grows and its surface area increases in the growth process. However, the radish is constantly grown while keeping its surface covered with lactic acid bacteria.

When the radish has been raised to a stage where it can be almost shipped, the radish is ready for shipment (step S).
9).

In such a method for cultivating soybean radish, an experiment was conducted to examine changes in the growth and reproductive state of lactic acid bacteria and various bacteria and Escherichia coli as an example of pathogenic bacteria over time. An example based on this experiment will be described with reference to FIG.

After washing and swelling the seeds, the test section S-1 performs only the lactic acid bacteria treatment described in step S3, and sprays the lactic acid bacteria in step S6 and the appropriate lactic acid bacteria sprayable in step S8. This is an example that is not performed.

After washing and swelling the seeds, the test section S-2 performs the lactic acid bacteria treatment described in step S3 and the lactic acid bacteria spraying in step S6, but does not perform the appropriate lactic acid bacteria spraying that can be performed in step S8. It is an example.

In the test section S-3, after washing and swelling the seeds, the lactic acid bacteria treatment described in the step S3 is not performed, the seeds are sown in the step S5, and the lactic acid bacteria are sprayed in the step S6. This is an example in which appropriate lactic acid bacteria spraying that can be performed in step S8 is not performed.

The control in the test section is an example in which no lactic acid bacterium treatment is performed for the purpose of a control experiment.

In the figure, the number of bacteria is expressed in units of cfu / g, and the portion indicated by reference numeral ND indicates a portion where no inspection is performed.

As shown, test plots S-1 and S-2
In E. coli, no coliform group was detected on days 0 to 6 of the elapsed days.

In the test plot S-3, the breeding state of the coliform bacteria was observed in accordance with the control in the test plot in which the lactic acid bacteria treatment was not performed.

These experimental results show that the treatment with lactic acid bacteria in step S3 immediately after washing and swelling the seeds is the most important and has an effect of suppressing the invasion and propagation of various bacteria and pathogenic bacteria.

That is, it is important that the surface of the seed is covered with lactic acid bacteria without any gaps before the germs and pathogens adhere to the surface of the seed. It turns out that the effect of suppressing invasion and propagation of various bacteria and pathogenic bacteria is high.

According to such an embodiment, the lactic acid bacteria are positively added after the seeds have been washed and swollen, whereby the invasion and propagation of various germs and pathogenic bacteria in the cultivation process of the plant can be suppressed, and thus, The possibility of food poisoning or the like can be eliminated. Moreover, since the lactic acid bacteria are attached to the plant, they are not only harmless to the human body but also can enjoy healthy effects such as intestinal regulation caused by the lactic acid bacteria.

In the above embodiment, an example in which radish was used as a plant was described. However, the present invention is not limited to this. For example, sprouts, three leaves, green onions, spinach, komatsuna, celery Needless to say, it can be widely applied to various kinds of plants such as tomato, cucumber, and spring chrysanthemum.

In the above-described embodiment, only lactic acid bacteria are added. However, if supplementary fungi such as natto bacteria are added in addition to lactic acid bacteria, the lactic acid bacteria and natto bacteria coexist with each other. Proliferation further increases the effect of synergistically inhibiting the invasion and propagation of various bacteria and pathogenic bacteria.

In the above description, lactic acid bacteria are taken as an example of fungi, but are not limited to lactic acid bacteria as long as they are harmless to the human body. Furthermore, it goes without saying that the auxiliary fungi described above are not limited to Bacillus natto. The above fungi is a concept including fungi and slime molds in eukaryotes and bacteria in prokaryotes.

[0052]

As described above, according to the present invention, invasion and propagation of various bacteria and pathogenic bacteria can be prevented.
It is a safe and inexpensive method for growing plants.

[Brief description of the drawings]

FIG. 1 is a flowchart showing a method of cultivating radish radish according to one embodiment of the present invention.

FIG. 2 is a chart showing an example of changes in the growth and reproductive status of lactic acid bacteria and coliform bacteria over time in the method for cultivating radish sprouts according to the embodiment.

FIG. 3 is a flowchart showing a conventional method for cultivating radish.

[Explanation of symbols]

 S1: washing of seeds S2: swelling S3: lactic acid bacteria treatment S4 ... rooting S5 ... sowing S6 ... spraying lactic acid bacteria S7 ... germination S8 ... growing

Claims (7)

[Claims] In a cultivation process of a plant in which a cultivation environment can be artificially controlled in a cultivation process from a seed stage to a shipping stage, the plant is washed to remove fungi harmless to the human body. A method for cultivating a plant, comprising fixing and growing the surface of the plant, and covering the surface of the plant with the fungus. 2. A method for cultivating a plant by hydroponics, wherein the plant is washed, fungi harmless to the human body are fixed on the surface of the plant and proliferated, and the surface of the plant is coated with the fungus. A method for cultivating a plant, comprising: 3. A method for cultivating a plant by hydroponics, wherein the plant is washed, fungi harmless to the human body are mixed into a culture solution and supplied to the plant, whereby the fungus is subjected to the method. Settled on the surface, including the roots of the plant, and proliferated,
A method for cultivating a plant, comprising coating a surface including a root of the plant with the fungus. 4. The fungus is harmless to the human body.
The method for cultivating a plant according to any one of claims 1 to 3, wherein the mixed fungus is a mixture of fungi of more than one kind, and the mixed fungi proliferate in symbiosis with each other. 5. The method according to claim 1, wherein the fungus is a lactic acid bacterium. 6. The colonization of the fungus on the surface of the plant,
The method for cultivating a plant according to claim 1, wherein the cleaning is performed without a long time after the plant is washed. 7. The plant to which the fungi are fixed after being washed is in any of a seed state, a rooted seed state, a germinated seed state, or a rooted and germinated seed state. The method for cultivating a plant according to claim 1, wherein the plant is cultivated.