JPH06239761A - Agent for internal use, food and drink having appetite stimulating action - Google Patents

Abstract

PURPOSE:To obtain an agent for internal use, food and drink having appetite stimulating action, exhibiting excellent appetite stimulating action different from conventional appetite stimulating agent by peroral administration and extremely convenient for use. CONSTITUTION:This agent for internal use is produced by extracting the fruit body of Agaricus blazei, (a kind of mushroom of the genus Agricus), its crushed product or its dried material with hot water and using the obtained composite component as active component. The food and drink contain this composite component.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to the genus Agaricus.
) Mushrooms (Agaricus blazei),
The present invention relates to an orally-administered agent and a food / beverage product having an appetite-stimulating action, which utilizes a composite component contained in so-called Himematsutake.

[0002]

2. Description of the Related Art Conventionally, as appetite enhancers, 1) bittering agents such as sea bream, oysters and oysters, 2) fragrances such as fennel, cinnamon, mint and the like, 3) cholinergic agents such as acetylcholine derivatives, 4) diastase. , Digestive fluid components such as pepsin, amylase and lipase, 5) acids such as dilute hydrochloric acid, citric acid and tartaric acid are known.

[0003]

The above-mentioned conventional appetite enhancers act on the periphery of sensory nerves of the tongue to promote the secretion of saliva or gastric juice, or by supplementing the digestive juice components themselves. It is what promotes. The present invention is different from the action of such conventional appetite enhancer, and a novel oral administration agent and food and drink that enhance appetite without promoting secretion of saliva or gastric juice and without supplementing digestive juice components themselves. Is provided.

[0004]

However, the present inventors have
As a result of diligent research to obtain a novel oral administration agent having an appetite-stimulating action different from conventional appetite enhancers, fruit bodies of Himematsutake mushrooms, crushed products or dried products thereof were extracted with hot water as the oral administration drug. It was found that the obtained composite component is correct and suitable, and most simply, the composite component can be contained in foods and drinks for use.

That is, the present invention relates to an orally administered agent having an appetite-increasing effect, which comprises as an active ingredient a complex component obtained by extracting hot fruit water from a fruiting body of Himematsutake mushroom, a crushed product thereof or a dried product thereof. The present invention relates to a food or drink containing the above composite component.

In the present invention, a composite component is obtained from fruit bodies of Himematsutake mushrooms. The target is a fruit body of Himematsutake mushroom, a crushed product thereof, or a dried product thereof, and the dried product is preferable from the viewpoints of storability, handleability and extraction efficiency.

In the present invention, fruit bodies of Himematsutake mushrooms, crushed products thereof, or dried products thereof are extracted with hot water. The target complex component is contained in the hot water extract. Prior to hot water extraction
It is also effective to extract the fruiting body of Himematsutake mushroom, its crushed product or its dried product with an organic solvent or a water-containing organic solvent to remove odorous components and pigment components specific to these.
Examples of the organic solvent used here include methanol, ethanol, ethyl acetate and ether, and examples of the water-containing organic solvent include water-containing methanol and water-containing ethanol which generally contain water in the range of 30% or less. About 80% ethanol is preferable in terms of the organic solvent.

It is also effective to subject the obtained hot water extract to alcohol precipitation, and further to purify by fractionating the alcohol precipitate by liquid chromatography. As the composite component of the present invention, a hot water extract, a vacuum concentrate thereof, or a lyophilized product thereof can be used, but it is preferable to use a lyophilized product purified as described above.

A complex component obtained by extracting a dried product of fruiting body of Himematsutake mushroom with an amount of hot water for 2 hours, and further concentrating the hot water extract under reduced pressure, fractionation by liquid chromatography, dialysis and freeze-drying. Has the following chemical composition, for example. Crude ash 5.54% (wt%, the same below), crude protein 43.19%, crude lipid 3.73
%, Crude fiber 6.01%, sugar 41.56%, ergosterol 0.14%. Further analysis of these crude ash content, crude protein, crude lipid and sugar results in Table 1, Table 2 and Table 3, respectively.
And the composition of Table 4.

[0010]

[Table 1]

[0011]

[Table 2]

[0012]

[Table 3]

In Table 3, the crude lipid has an acid value of 33.7,
Peroxide value 1.2, Carbonyl value 13.6, Saponification value 20
3.5, iodine value 142.6, crude lipid is neutral fat 4
It is composed of 4.4%, phospholipids 38.7% and glycolipids 16.9%. The triglyceride is 27.9.
%, Sterol 29.2%, free fatty acid 19.6%, diglyceride 10.3%, monoglyceride 7.4%, sterol ester 5.5% and others 0.1%. The phospholipids were phosphatidylethanolamine 45.8%, phosphatidylcholine + lysophosphatidylethanolamine 31.2%, cardiolipin 9.
5%, lysophosphatidylcholine + phosphatidylserine 12.3%, and other 1.2%.

[0014]

[Table 4]

The composite component of the present invention having the composition as described above does not show a fixed decomposition point and melting point and is carbonized by strong heat, but is extremely stable. It is stable at room temperature for at least 3 years, and its activity is not decreased even after sterilization at 120 ° C. for 20 minutes.

The complex component of the present invention shows PH6.5 which is generally known not to exhibit gastric mucosa stimulating action, and has an appetite enhancing action different from conventional appetite enhancers. The complex component does not promote the secretion of saliva or gastric juice, does not supplement the digestive juice component itself, and promotes the movement of the stomach and especially the large intestine without affecting the movement of the small intestine. It is known that the fruiting body of Pleurotus ostreatus contains an anti-cancer active ingredient (Japanese Patent Laid-Open No. 64-6719).
4, JP-A-64-67195, JP-A-2-7863
0), and it is also known that it contains a liver function improving component (JP-A-2-124829), but it is not known that it contains a complex component having an appetite enhancing action as described above. .

The present invention relates to an orally-administered agent having an appetite-stimulating action, which comprises the above-described composite ingredient as an active ingredient,
It also relates to foods and drinks containing the composite ingredient. The simplest method of providing the complex component of the present invention as an oral administration agent having an appetite-stimulating action is a method of providing the complex component as a food or drink.

There are various methods for providing the complex component of the present invention as food and drink, as follows. 1) The hot water extract as described above, the concentrated solution thereof or the freeze-dried product thereof is used as it is as a sprinkle or filled in a tea pack or a capsule and used 2) The hot water extract as described above or the same Method of mixing sugars, acids, salts, flavors and the like in a vacuum concentrate and using it 3) The hot water extract as described above, its vacuum concentrate or its freeze-dried product is baked, fermented product, paste, milk Product,
A method of adding oils and fats, seasonings, foods such as confectionery, or beverages such as coffee, cocoa, tea, fruit juice, vegetable juice, fermented beverages, soft drinks, etc.

[0019]

【Example】

Test Category 1 (separation of composite components and evaluation thereof) -Example 1 Crushing fruit bodies of Himematsutake mushrooms and drying, 1000 ml of purified water was added to 100 g of the dried product, and gently stirred for 2 hours on a water bath, It was extracted with hot water. The same operation was repeated twice, and the hot water extracts were combined twice and then concentrated under reduced pressure to 200 ml. Ethanol was added to the vacuum concentrate until the final ethanol concentration reached 70%, centrifuged and
9.3 g of ethanol precipitate was isolated. DEAE-Toyopearl gel (trade name, with ethanol precipitate as stationary phase)
It was subjected to column chromatography packed with Toyo Soda Kogyo Co., Ltd., and eluted by the phenol-sulfuric acid method until the color of the sugar disappeared, and the eluted fraction was fractionated. The eluted fraction was dialyzed and then freeze-dried to obtain 4.5 g of a composite component. The obtained composite component had the above composition and physicochemical properties.

The appetite-promoting effect of oral administration of the thus obtained composite ingredient was evaluated as follows.

ICR mice were preliminarily bred for 2 weeks with MF (trade name, manufactured by Oriental Yeast Co., Ltd.), which is a standard feeding powder, and healthy ones with good growth were used. Ten mice (male, female) in each group were used and preliminarily bred for 2 weeks as described above, then the temperature was 23 ± 2 ° C. and the relative temperature was 55.
Under the environment of ± 5%, tap water is freely ingested, while the control group continues to receive MF, and the sample group contains 10% of the complex component.
The contained MF was freely taken. The mice were bred for a total of 10 weeks including the preliminary breeding, and the body weight of the mice and the weekly feed intake were measured to calculate the average body weight value (g) and the average weekly feed intake (g) of 10 mice. The results are shown in Tables 5 and 6.

[0022]

[Table 5]

[0023]

[Table 6]

At the same time, fresh urine collected from each group at 5 weeks, 7 weeks and 9 weeks was subjected to PH, protein, glucose,
Ketone bodies, occult blood reaction, urobilinogen and bilirubin were measured, and Na, K, Ca and P were also measured, but no significant difference was observed between the control group and the sample group.

After blood was collected from the sample group after 10 weeks,
All individuals were dissected, the wet weights of the organs of each individual were measured, and the histopathological examination was also performed. No abnormalities due to the administration of the complex components were observed.

Further, an acute toxicity test was carried out by oral administration of the complex components, and LD50 for mice was 3000 mg.
> / Kg, LD50 for rats is 2500 mg /
It was over kg. Also, from the results of the subacute toxicity test on rats and the results of the general pharmacology test on rabbits, the complex component of the present invention did not have a problem regarding toxicity.

Separately, in order to evaluate the effect of the complex components on the digestive tract motility, the action of Hartely type guinea pigs (body weight 500 g, male) on the digestive tract motility was evaluated by the method of Hitoshi Ito et al. Pages 304-315,
1970, Mie Medical J
ournal), Vol. 14, No. 1, 47-68, 1964}. That is, a guinea pig under urethane anesthesia is floated in Tyrode's solution (nutrient solution) in a 37 ° C thermostat, and its stomach, large intestine (descending colon) and small intestine (ileum) are fixed, and these are fixed with special serphins. The suspension was suspended, the movement was drawn on soot paper, and the complex components were orally injected by a gastric tube to observe changes in digestive tract movement.

FIG. 1 is a diagram showing the contraction of the large intestine and small intestine motility among them, the horizontal axis represents the time (minutes) after the oral infusion of the complex components, and the vertical axis represents the large intestine and small intestine motility. The contraction (cm), the upper part of the figure shows the contraction of colonic movement,
The lower part shows contraction of small intestinal motility.

5 minutes after the oral administration of 5 ml of the 10% solution of the complex component, the gastric amplitude and the number of amplitudes increased and the tone increased 5 to 10 minutes after the injection, and the marked increase in the amplitude of the large intestine after 35 minutes. And increased tone, respectively, and moderate increase in gastric motility and marked increase in large intestinal motility were observed, but there was no effect on small intestinal motility.

Test Category 2 (Production of Food and Beverages) Example 2 After obtaining a hot water extract in the same manner as in Example 1, 1 kg of the hot water extract had 100 g of sugar, 15 g of honey and 5 caramel.
g, 0.75 g of ascorbic acid, 0.3 g of citric acid, and 0.2 g of a lemon fragrance were prepared to produce a health drink.

Example 3 After obtaining an ethanol precipitate in the same manner as in Example 1, 10 g of the freeze-dried product of the ethanol precipitate and 0.5 g of ascorbic acid were mixed into 2 kg of apple juice to prepare apple juice. Was manufactured.

Example 4 After obtaining a freeze-dried composite component in the same manner as in Example 1, 5 g of the composite component was minced with a small amount of calcium chloride and sodium triphosphate and exposed to water. After dehydration, it was added to 2 kg of pre-chilled fish meat in the mashing step and frozen to produce a frozen ground taste.

[0033]

EFFECTS OF THE INVENTION As is apparent from the above, the present invention described above has an excellent effect on appetite which is different from the conventional appetite enhancers by oral administration, and has the effect of being extremely simple in its specific use. is there.

[Brief description of drawings]

FIG. 1 is a diagram showing contraction of large intestine and small intestine motility when the composite ingredient of the present invention is orally injected into a guinea pig.

Claims (2)

[Claims] 1. An orally-administered agent having an appetite-increasing effect, which comprises as an active ingredient a complex component obtained by extracting hot fruit water from a fruiting body of Himematsutake mushroom, a crushed product thereof, or a dried product thereof. 2. A food or drink comprising a fruiting body of Himematsutake mushroom, a crushed product thereof, or a composite component obtained by extracting the dried product with hot water.