JPH057995B2 - - Google Patents
Info
- Publication number
- JPH057995B2 JPH057995B2 JP59134536A JP13453684A JPH057995B2 JP H057995 B2 JPH057995 B2 JP H057995B2 JP 59134536 A JP59134536 A JP 59134536A JP 13453684 A JP13453684 A JP 13453684A JP H057995 B2 JPH057995 B2 JP H057995B2
- Authority
- JP
- Japan
- Prior art keywords
- dna
- growth hormone
- added
- tris
- cdna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108020004414 DNA Proteins 0.000 claims description 67
- 108010051696 Growth Hormone Proteins 0.000 claims description 34
- 102000018997 Growth Hormone Human genes 0.000 claims description 33
- 239000013612 plasmid Substances 0.000 claims description 25
- 241000251468 Actinopterygii Species 0.000 claims description 22
- 241000588724 Escherichia coli Species 0.000 claims description 15
- 108020004511 Recombinant DNA Proteins 0.000 claims description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 30
- 239000000243 solution Substances 0.000 description 27
- 239000000122 growth hormone Substances 0.000 description 25
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 22
- 235000019688 fish Nutrition 0.000 description 21
- 238000000034 method Methods 0.000 description 21
- 239000002299 complementary DNA Substances 0.000 description 20
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 17
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 16
- 239000000872 buffer Substances 0.000 description 16
- 239000011780 sodium chloride Substances 0.000 description 15
- 241000277329 Oncorhynchus keta Species 0.000 description 14
- -1 In particular Proteins 0.000 description 13
- 206010062767 Hypophysitis Diseases 0.000 description 10
- 108020004999 messenger RNA Proteins 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- 210000003635 pituitary gland Anatomy 0.000 description 10
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 8
- 125000003275 alpha amino acid group Chemical group 0.000 description 8
- 229910001629 magnesium chloride Inorganic materials 0.000 description 8
- 244000005700 microbiome Species 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 108091036060 Linker DNA Proteins 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 7
- 238000012869 ethanol precipitation Methods 0.000 description 7
- 241000972773 Aulopiformes Species 0.000 description 6
- 108091034057 RNA (poly(A)) Proteins 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 239000007795 chemical reaction product Substances 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 235000019515 salmon Nutrition 0.000 description 6
- 239000003298 DNA probe Substances 0.000 description 5
- 229920002678 cellulose Polymers 0.000 description 5
- 239000001913 cellulose Substances 0.000 description 5
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 5
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 5
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 108020003215 DNA Probes Proteins 0.000 description 4
- 102100034343 Integrase Human genes 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000010804 cDNA synthesis Methods 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 3
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 3
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000002205 phenol-chloroform extraction Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108010000521 Human Growth Hormone Proteins 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 150000002540 isothiocyanates Chemical class 0.000 description 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000881711 Acipenser sturio Species 0.000 description 1
- 241001385733 Aesculus indica Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101900063352 Escherichia coli DNA ligase Proteins 0.000 description 1
- 101900242680 Escherichia coli Ribonuclease H Proteins 0.000 description 1
- 241001524679 Escherichia virus M13 Species 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- 101710203526 Integrase Proteins 0.000 description 1
- KVSNNYRCFPXKGH-UHFFFAOYSA-M N#C[S-].Cl.[Cs+] Chemical compound N#C[S-].Cl.[Cs+] KVSNNYRCFPXKGH-UHFFFAOYSA-M 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 description 1
- 101000868151 Rattus norvegicus Somatotropin Proteins 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 108010077895 Sarcosine Proteins 0.000 description 1
- 101000868144 Sus scrofa Somatotropin Proteins 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 241000276707 Tilapia Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 108010006025 bovine growth hormone Proteins 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 108091092330 cytoplasmic RNA Proteins 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229950006238 nadide Drugs 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 229940043230 sarcosine Drugs 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 241001223854 teleost fish Species 0.000 description 1
- 125000002480 thymidyl group Chemical group 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 150000005691 triesters Chemical class 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Endocrinology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Feed For Specific Animals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59134536A JPS6115699A (ja) | 1984-06-29 | 1984-06-29 | 魚類の成長ホルモン遺伝子 |
| CA000485108A CA1272144A (fr) | 1984-06-29 | 1985-06-25 | Hormone de croissance polypeptidique pour les poissons |
| NO852568A NO174717C (no) | 1984-06-29 | 1985-06-26 | Fremgangsmåte for fremstilling av et fiskeveksthormon-polypeptid fra Oncorhynchus keta samt ekspresjonsvektorer til bruk ved fremgangsmåten |
| AU44195/85A AU575961B2 (en) | 1984-06-29 | 1985-06-26 | Salmon fish growth hormone by genetic engeneering |
| EP85107987A EP0166444B1 (fr) | 1984-06-29 | 1985-06-27 | Polypeptide de l'hormone de croissance de poisson |
| SU853913602A RU1825376C (ru) | 1984-06-29 | 1985-06-28 | Способ получени ДНК, кодирующей гормон роста лосос , способ конст-ни промежуточной рекомбинантной плазмидной ДНК, содержащей ДНК-фрагмент, кодирующий гормон роста лосос , дл получени плазмид pS GH1, pS GH3, pS GH9, pS GH10, pS GH14 и pS GH17, способ получени реком-ной плазмидной ДНК pS GH1B2, кодирующей гормон роста лосос , способ получени реком-ной плазмидной ДНК pSGH II С2, кодирующей гормон роста лосос , способ получени реком-ного гормона роста лосос |
| US06/750,587 US4689402A (en) | 1984-06-29 | 1985-07-01 | Fish growth hormone polypeptide |
| US07/017,630 US4849359A (en) | 1984-06-29 | 1987-04-14 | Fish growth hormone polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59134536A JPS6115699A (ja) | 1984-06-29 | 1984-06-29 | 魚類の成長ホルモン遺伝子 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6115699A JPS6115699A (ja) | 1986-01-23 |
| JPH057995B2 true JPH057995B2 (fr) | 1993-01-29 |
Family
ID=15130608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59134536A Granted JPS6115699A (ja) | 1984-06-29 | 1984-06-29 | 魚類の成長ホルモン遺伝子 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6115699A (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6193197A (ja) * | 1984-10-12 | 1986-05-12 | Kyowa Hakko Kogyo Co Ltd | 魚類の成長ホルモンポリペプチド |
| JPS6193196A (ja) * | 1984-10-12 | 1986-05-12 | Kyowa Hakko Kogyo Co Ltd | 魚類の成長ホルモン遺伝子 |
| JPH0568572A (ja) * | 1991-09-11 | 1993-03-23 | Agency Of Ind Science & Technol | サケ成長ホルモンを生産するラン藻シネココツカス |
-
1984
- 1984-06-29 JP JP59134536A patent/JPS6115699A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6115699A (ja) | 1986-01-23 |
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